Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity

Autores
Joray, Mariana Belén; Palacios, Sara Maria; Carpinella, Maria Cecilia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As part of our ongoing research on the antibacterial activity of Achyrocline satureioides, this study seeks to better understand the interactions between the metabolites isolated from this plant. For this purpose, the combined effect of 23-methyl-6-O-desmethylauricepyrone (1), quercetin (2) and 3-O-methylquercetin (3), obtained through bioguided fractionation from A. satureioides ethanol extract, was evaluated against Staphylococcus aureus and Escherichia coli. In first place, the antibacterial effect of the combination of flavonols 2 and 3 was assessed, as these showed individual effectiveness lower than or equal to that of the fraction from which they were obtained. When the flavonols were applied together at concentrations below their minimum inhibitory concentration (MIC) values, a synergistic effect (FICI < 0.30) against S. aureus was observed. In addition, compounds 2 and 3 in combination reduced 1000 times the MIC of compound 1, showing a clear synergistic interaction (FICI < 0.15) in treatments against the Gram (+) bacterium. The most active combination against E. coli showed an additive interaction (FICI < 0.62) between the three assayed compounds 1–3. These results indicated the existence of concerted action between these metabolites, evidence of the importance of the synergistic interactions between the components of plant-derived extracts for the control of pathogenic bacteria.
Fil: Joray, Mariana Belén. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacios, Sara Maria. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Achyrocline Satureioides
Sinergismo
Antibacterianos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22689
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activityJoray, Mariana BelénPalacios, Sara MariaCarpinella, Maria CeciliaAchyrocline SatureioidesSinergismoAntibacterianoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1As part of our ongoing research on the antibacterial activity of Achyrocline satureioides, this study seeks to better understand the interactions between the metabolites isolated from this plant. For this purpose, the combined effect of 23-methyl-6-O-desmethylauricepyrone (1), quercetin (2) and 3-O-methylquercetin (3), obtained through bioguided fractionation from A. satureioides ethanol extract, was evaluated against Staphylococcus aureus and Escherichia coli. In first place, the antibacterial effect of the combination of flavonols 2 and 3 was assessed, as these showed individual effectiveness lower than or equal to that of the fraction from which they were obtained. When the flavonols were applied together at concentrations below their minimum inhibitory concentration (MIC) values, a synergistic effect (FICI < 0.30) against S. aureus was observed. In addition, compounds 2 and 3 in combination reduced 1000 times the MIC of compound 1, showing a clear synergistic interaction (FICI < 0.15) in treatments against the Gram (+) bacterium. The most active combination against E. coli showed an additive interaction (FICI < 0.62) between the three assayed compounds 1–3. These results indicated the existence of concerted action between these metabolites, evidence of the importance of the synergistic interactions between the components of plant-derived extracts for the control of pathogenic bacteria.Fil: Joray, Mariana Belén. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palacios, Sara Maria. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Gmbh2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22689Joray, Mariana Belén; Palacios, Sara Maria; Carpinella, Maria Cecilia; Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity; Elsevier Gmbh; Phytomedicine; 20; 3-4; 12-2012; 258-2610944-7113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.10.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711312003819info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:44Zoai:ri.conicet.gov.ar:11336/22689instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:44.821CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
title Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
spellingShingle Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
Joray, Mariana Belén
Achyrocline Satureioides
Sinergismo
Antibacterianos
title_short Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
title_full Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
title_fullStr Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
title_full_unstemmed Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
title_sort Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity
dc.creator.none.fl_str_mv Joray, Mariana Belén
Palacios, Sara Maria
Carpinella, Maria Cecilia
author Joray, Mariana Belén
author_facet Joray, Mariana Belén
Palacios, Sara Maria
Carpinella, Maria Cecilia
author_role author
author2 Palacios, Sara Maria
Carpinella, Maria Cecilia
author2_role author
author
dc.subject.none.fl_str_mv Achyrocline Satureioides
Sinergismo
Antibacterianos
topic Achyrocline Satureioides
Sinergismo
Antibacterianos
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv As part of our ongoing research on the antibacterial activity of Achyrocline satureioides, this study seeks to better understand the interactions between the metabolites isolated from this plant. For this purpose, the combined effect of 23-methyl-6-O-desmethylauricepyrone (1), quercetin (2) and 3-O-methylquercetin (3), obtained through bioguided fractionation from A. satureioides ethanol extract, was evaluated against Staphylococcus aureus and Escherichia coli. In first place, the antibacterial effect of the combination of flavonols 2 and 3 was assessed, as these showed individual effectiveness lower than or equal to that of the fraction from which they were obtained. When the flavonols were applied together at concentrations below their minimum inhibitory concentration (MIC) values, a synergistic effect (FICI < 0.30) against S. aureus was observed. In addition, compounds 2 and 3 in combination reduced 1000 times the MIC of compound 1, showing a clear synergistic interaction (FICI < 0.15) in treatments against the Gram (+) bacterium. The most active combination against E. coli showed an additive interaction (FICI < 0.62) between the three assayed compounds 1–3. These results indicated the existence of concerted action between these metabolites, evidence of the importance of the synergistic interactions between the components of plant-derived extracts for the control of pathogenic bacteria.
Fil: Joray, Mariana Belén. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacios, Sara Maria. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description As part of our ongoing research on the antibacterial activity of Achyrocline satureioides, this study seeks to better understand the interactions between the metabolites isolated from this plant. For this purpose, the combined effect of 23-methyl-6-O-desmethylauricepyrone (1), quercetin (2) and 3-O-methylquercetin (3), obtained through bioguided fractionation from A. satureioides ethanol extract, was evaluated against Staphylococcus aureus and Escherichia coli. In first place, the antibacterial effect of the combination of flavonols 2 and 3 was assessed, as these showed individual effectiveness lower than or equal to that of the fraction from which they were obtained. When the flavonols were applied together at concentrations below their minimum inhibitory concentration (MIC) values, a synergistic effect (FICI < 0.30) against S. aureus was observed. In addition, compounds 2 and 3 in combination reduced 1000 times the MIC of compound 1, showing a clear synergistic interaction (FICI < 0.15) in treatments against the Gram (+) bacterium. The most active combination against E. coli showed an additive interaction (FICI < 0.62) between the three assayed compounds 1–3. These results indicated the existence of concerted action between these metabolites, evidence of the importance of the synergistic interactions between the components of plant-derived extracts for the control of pathogenic bacteria.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22689
Joray, Mariana Belén; Palacios, Sara Maria; Carpinella, Maria Cecilia; Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity; Elsevier Gmbh; Phytomedicine; 20; 3-4; 12-2012; 258-261
0944-7113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22689
identifier_str_mv Joray, Mariana Belén; Palacios, Sara Maria; Carpinella, Maria Cecilia; Understanding the interactions between metabolites isolated from Achyrocline satureioides in relation to its antibacterial activity; Elsevier Gmbh; Phytomedicine; 20; 3-4; 12-2012; 258-261
0944-7113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.10.015
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711312003819
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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