Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria

Autores
Lores Arnaiz, Silvia; Bustamante, Jaunita
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brain aging has been associated with mitochondrial dysfunction and changes in nitric oxide levels. The aim of this study was to evaluate the susceptibility of synaptic and non-synaptic mitochondria to aging-dependent dysfunction. State 3 respiratory rate and respiratory control were 43% and 33% decreased, respectively in brain cortex synaptosomes from 14-month-old animals, as compared with synaptosomes from 3-month-old mice. Respiratory rates were not significantly affected by aging in non-synaptic mitochondrial fractions. Mitochondrial dysfunction was associated with increases of 84% and 38% in H 2O 2 production rates in brain cortex synaptosomes and non-synaptic mitochondria, respectively, from 14-month-old mice, as compared with young animals. Synaptic mitochondria seem to be more susceptible to calcium insult in 14-month-old mice, as compared with non-synaptic mitochondria, as measured by response of both types of fractions to calcium-induced depolarization. With aging, nitric oxide (NO) production was 44% and 27% decreased both in synaptosomal and non-synaptic mitochondrial fractions, respectively. The results of this study suggest that with aging, mitochondrial function at the nerve terminals would be more susceptible to suffer alterations by the constant calcium changes occurring as a consequence of synaptic activity. Non-synaptic mitochondria would be more resistant to age-related dysfunction and oxidative damage. © 2011 IBRO.
Fil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Bustamante, Jaunita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Aging
Cerebral Cortex
Mitochondrial Respiration
Nitric Oxide Synthase
Non-Synaptic Mitochondria
Synaptosomes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67547
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67547
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondriaLores Arnaiz, SilviaBustamante, JaunitaAgingCerebral CortexMitochondrial RespirationNitric Oxide SynthaseNon-Synaptic MitochondriaSynaptosomeshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Brain aging has been associated with mitochondrial dysfunction and changes in nitric oxide levels. The aim of this study was to evaluate the susceptibility of synaptic and non-synaptic mitochondria to aging-dependent dysfunction. State 3 respiratory rate and respiratory control were 43% and 33% decreased, respectively in brain cortex synaptosomes from 14-month-old animals, as compared with synaptosomes from 3-month-old mice. Respiratory rates were not significantly affected by aging in non-synaptic mitochondrial fractions. Mitochondrial dysfunction was associated with increases of 84% and 38% in H 2O 2 production rates in brain cortex synaptosomes and non-synaptic mitochondria, respectively, from 14-month-old mice, as compared with young animals. Synaptic mitochondria seem to be more susceptible to calcium insult in 14-month-old mice, as compared with non-synaptic mitochondria, as measured by response of both types of fractions to calcium-induced depolarization. With aging, nitric oxide (NO) production was 44% and 27% decreased both in synaptosomal and non-synaptic mitochondrial fractions, respectively. The results of this study suggest that with aging, mitochondrial function at the nerve terminals would be more susceptible to suffer alterations by the constant calcium changes occurring as a consequence of synaptic activity. Non-synaptic mitochondria would be more resistant to age-related dysfunction and oxidative damage. © 2011 IBRO.Fil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Bustamante, Jaunita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaPergamon-Elsevier Science Ltd2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67547Lores Arnaiz, Silvia; Bustamante, Jaunita; Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria; Pergamon-Elsevier Science Ltd; Neuroscience; 188; 8-2011; 117-1240306-4522CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306452211005203info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2011.04.060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:30Zoai:ri.conicet.gov.ar:11336/67547instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:30.365CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
title Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
spellingShingle Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
Lores Arnaiz, Silvia
Aging
Cerebral Cortex
Mitochondrial Respiration
Nitric Oxide Synthase
Non-Synaptic Mitochondria
Synaptosomes
title_short Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
title_full Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
title_fullStr Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
title_full_unstemmed Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
title_sort Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria
dc.creator.none.fl_str_mv Lores Arnaiz, Silvia
Bustamante, Jaunita
author Lores Arnaiz, Silvia
author_facet Lores Arnaiz, Silvia
Bustamante, Jaunita
author_role author
author2 Bustamante, Jaunita
author2_role author
dc.subject.none.fl_str_mv Aging
Cerebral Cortex
Mitochondrial Respiration
Nitric Oxide Synthase
Non-Synaptic Mitochondria
Synaptosomes
topic Aging
Cerebral Cortex
Mitochondrial Respiration
Nitric Oxide Synthase
Non-Synaptic Mitochondria
Synaptosomes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Brain aging has been associated with mitochondrial dysfunction and changes in nitric oxide levels. The aim of this study was to evaluate the susceptibility of synaptic and non-synaptic mitochondria to aging-dependent dysfunction. State 3 respiratory rate and respiratory control were 43% and 33% decreased, respectively in brain cortex synaptosomes from 14-month-old animals, as compared with synaptosomes from 3-month-old mice. Respiratory rates were not significantly affected by aging in non-synaptic mitochondrial fractions. Mitochondrial dysfunction was associated with increases of 84% and 38% in H 2O 2 production rates in brain cortex synaptosomes and non-synaptic mitochondria, respectively, from 14-month-old mice, as compared with young animals. Synaptic mitochondria seem to be more susceptible to calcium insult in 14-month-old mice, as compared with non-synaptic mitochondria, as measured by response of both types of fractions to calcium-induced depolarization. With aging, nitric oxide (NO) production was 44% and 27% decreased both in synaptosomal and non-synaptic mitochondrial fractions, respectively. The results of this study suggest that with aging, mitochondrial function at the nerve terminals would be more susceptible to suffer alterations by the constant calcium changes occurring as a consequence of synaptic activity. Non-synaptic mitochondria would be more resistant to age-related dysfunction and oxidative damage. © 2011 IBRO.
Fil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Bustamante, Jaunita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Brain aging has been associated with mitochondrial dysfunction and changes in nitric oxide levels. The aim of this study was to evaluate the susceptibility of synaptic and non-synaptic mitochondria to aging-dependent dysfunction. State 3 respiratory rate and respiratory control were 43% and 33% decreased, respectively in brain cortex synaptosomes from 14-month-old animals, as compared with synaptosomes from 3-month-old mice. Respiratory rates were not significantly affected by aging in non-synaptic mitochondrial fractions. Mitochondrial dysfunction was associated with increases of 84% and 38% in H 2O 2 production rates in brain cortex synaptosomes and non-synaptic mitochondria, respectively, from 14-month-old mice, as compared with young animals. Synaptic mitochondria seem to be more susceptible to calcium insult in 14-month-old mice, as compared with non-synaptic mitochondria, as measured by response of both types of fractions to calcium-induced depolarization. With aging, nitric oxide (NO) production was 44% and 27% decreased both in synaptosomal and non-synaptic mitochondrial fractions, respectively. The results of this study suggest that with aging, mitochondrial function at the nerve terminals would be more susceptible to suffer alterations by the constant calcium changes occurring as a consequence of synaptic activity. Non-synaptic mitochondria would be more resistant to age-related dysfunction and oxidative damage. © 2011 IBRO.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67547
Lores Arnaiz, Silvia; Bustamante, Jaunita; Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria; Pergamon-Elsevier Science Ltd; Neuroscience; 188; 8-2011; 117-124
0306-4522
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67547
identifier_str_mv Lores Arnaiz, Silvia; Bustamante, Jaunita; Age-related alterations in mitochondrial physiological parameters and nitric oxide production in synaptic and non-synaptic brain cortex mitochondria; Pergamon-Elsevier Science Ltd; Neuroscience; 188; 8-2011; 117-124
0306-4522
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306452211005203
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2011.04.060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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