Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment

Autores
Lores Arnaiz, Silvia; Lores Arnaiz, Maria del Rosario; Czerniczyniec, Analia; Cuello, M; Bustamante, Joanita
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Male rats (21 days) were assigned to enriched environment (EE) or to standard environment (SE) for 1 year. Oxygen consumption and the sensitivity to calcium induced mitochondrial permeability transition (MPT), through mitochondrial membrane potential (ΔΨm) and swelling, were determined in isolated hippocampal and cerebral cortex mitochondria. Mitochondrial H2O2 production rate, and NOS activity and expression associated with mitochondrial membranes were also assayed. Results showed that state 3 respiratory rate was increased by 80% in cerebral cortex mitochondria from EE rats and no changes were observed in hippocampal mitochondria after EE exposure. Calcium induced-swelling was 40% and 53% lower in hippocampal and cerebral cortex mitochondria from EE rats, as compared with SE rats. Calcium loading induced membrane depolarization in cerebral cortex mitochondria from EE rats but did not affect mitochondrial ΔΨm in hippocampal mitochondria from EE animals, probably due to decreased H2O2 formation. NO production associated to mitochondrial membranes was increased by 195% in cerebral cortex mitochondria but decreased by 47% in hippocampal mitochondria from EE rats, as compared with SE rats. Western blot analysis from nNOS protein expression associated to mitochondrial samples revealed a similar pattern. Our results suggest that in hippocampus and cerebral cortex, EE exposure protects mitochondria against calcium-induced MPT maintaining a convenient membrane potential, which assures a continuous energy supply.
Fil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Lores Arnaiz, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Psicología; Argentina
Fil: Czerniczyniec, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Cuello, M. Universidad de Buenos Aires. Facultad de Psicología; Argentina
Fil: Bustamante, Joanita. Universidad de Buenos Aires. Facultad de Psicología; Argentina
Materia
CEREBRAL CORTEX
ENRICHED ENVIRONMENT
HIPPOCAMPUS
MITOCHONDRIA
NITRIC OXIDE SYNTHASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151512
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/151512
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environmentLores Arnaiz, SilviaLores Arnaiz, Maria del RosarioCzerniczyniec, AnaliaCuello, MBustamante, JoanitaCEREBRAL CORTEXENRICHED ENVIRONMENTHIPPOCAMPUSMITOCHONDRIANITRIC OXIDE SYNTHASEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Male rats (21 days) were assigned to enriched environment (EE) or to standard environment (SE) for 1 year. Oxygen consumption and the sensitivity to calcium induced mitochondrial permeability transition (MPT), through mitochondrial membrane potential (ΔΨm) and swelling, were determined in isolated hippocampal and cerebral cortex mitochondria. Mitochondrial H2O2 production rate, and NOS activity and expression associated with mitochondrial membranes were also assayed. Results showed that state 3 respiratory rate was increased by 80% in cerebral cortex mitochondria from EE rats and no changes were observed in hippocampal mitochondria after EE exposure. Calcium induced-swelling was 40% and 53% lower in hippocampal and cerebral cortex mitochondria from EE rats, as compared with SE rats. Calcium loading induced membrane depolarization in cerebral cortex mitochondria from EE rats but did not affect mitochondrial ΔΨm in hippocampal mitochondria from EE animals, probably due to decreased H2O2 formation. NO production associated to mitochondrial membranes was increased by 195% in cerebral cortex mitochondria but decreased by 47% in hippocampal mitochondria from EE rats, as compared with SE rats. Western blot analysis from nNOS protein expression associated to mitochondrial samples revealed a similar pattern. Our results suggest that in hippocampus and cerebral cortex, EE exposure protects mitochondria against calcium-induced MPT maintaining a convenient membrane potential, which assures a continuous energy supply.Fil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Lores Arnaiz, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Psicología; ArgentinaFil: Czerniczyniec, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Cuello, M. Universidad de Buenos Aires. Facultad de Psicología; ArgentinaFil: Bustamante, Joanita. Universidad de Buenos Aires. Facultad de Psicología; ArgentinaElsevier Science2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151512Lores Arnaiz, Silvia; Lores Arnaiz, Maria del Rosario; Czerniczyniec, Analia; Cuello, M; Bustamante, Joanita; Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment; Elsevier Science; Brain Research; 1319; 3-2010; 44-530006-8993CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006899310000570info:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainres.2010.01.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:09Zoai:ri.conicet.gov.ar:11336/151512instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:09.324CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
title Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
spellingShingle Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
Lores Arnaiz, Silvia
CEREBRAL CORTEX
ENRICHED ENVIRONMENT
HIPPOCAMPUS
MITOCHONDRIA
NITRIC OXIDE SYNTHASE
title_short Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
title_full Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
title_fullStr Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
title_full_unstemmed Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
title_sort Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment
dc.creator.none.fl_str_mv Lores Arnaiz, Silvia
Lores Arnaiz, Maria del Rosario
Czerniczyniec, Analia
Cuello, M
Bustamante, Joanita
author Lores Arnaiz, Silvia
author_facet Lores Arnaiz, Silvia
Lores Arnaiz, Maria del Rosario
Czerniczyniec, Analia
Cuello, M
Bustamante, Joanita
author_role author
author2 Lores Arnaiz, Maria del Rosario
Czerniczyniec, Analia
Cuello, M
Bustamante, Joanita
author2_role author
author
author
author
dc.subject.none.fl_str_mv CEREBRAL CORTEX
ENRICHED ENVIRONMENT
HIPPOCAMPUS
MITOCHONDRIA
NITRIC OXIDE SYNTHASE
topic CEREBRAL CORTEX
ENRICHED ENVIRONMENT
HIPPOCAMPUS
MITOCHONDRIA
NITRIC OXIDE SYNTHASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Male rats (21 days) were assigned to enriched environment (EE) or to standard environment (SE) for 1 year. Oxygen consumption and the sensitivity to calcium induced mitochondrial permeability transition (MPT), through mitochondrial membrane potential (ΔΨm) and swelling, were determined in isolated hippocampal and cerebral cortex mitochondria. Mitochondrial H2O2 production rate, and NOS activity and expression associated with mitochondrial membranes were also assayed. Results showed that state 3 respiratory rate was increased by 80% in cerebral cortex mitochondria from EE rats and no changes were observed in hippocampal mitochondria after EE exposure. Calcium induced-swelling was 40% and 53% lower in hippocampal and cerebral cortex mitochondria from EE rats, as compared with SE rats. Calcium loading induced membrane depolarization in cerebral cortex mitochondria from EE rats but did not affect mitochondrial ΔΨm in hippocampal mitochondria from EE animals, probably due to decreased H2O2 formation. NO production associated to mitochondrial membranes was increased by 195% in cerebral cortex mitochondria but decreased by 47% in hippocampal mitochondria from EE rats, as compared with SE rats. Western blot analysis from nNOS protein expression associated to mitochondrial samples revealed a similar pattern. Our results suggest that in hippocampus and cerebral cortex, EE exposure protects mitochondria against calcium-induced MPT maintaining a convenient membrane potential, which assures a continuous energy supply.
Fil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Lores Arnaiz, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Psicología; Argentina
Fil: Czerniczyniec, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Cuello, M. Universidad de Buenos Aires. Facultad de Psicología; Argentina
Fil: Bustamante, Joanita. Universidad de Buenos Aires. Facultad de Psicología; Argentina
description Male rats (21 days) were assigned to enriched environment (EE) or to standard environment (SE) for 1 year. Oxygen consumption and the sensitivity to calcium induced mitochondrial permeability transition (MPT), through mitochondrial membrane potential (ΔΨm) and swelling, were determined in isolated hippocampal and cerebral cortex mitochondria. Mitochondrial H2O2 production rate, and NOS activity and expression associated with mitochondrial membranes were also assayed. Results showed that state 3 respiratory rate was increased by 80% in cerebral cortex mitochondria from EE rats and no changes were observed in hippocampal mitochondria after EE exposure. Calcium induced-swelling was 40% and 53% lower in hippocampal and cerebral cortex mitochondria from EE rats, as compared with SE rats. Calcium loading induced membrane depolarization in cerebral cortex mitochondria from EE rats but did not affect mitochondrial ΔΨm in hippocampal mitochondria from EE animals, probably due to decreased H2O2 formation. NO production associated to mitochondrial membranes was increased by 195% in cerebral cortex mitochondria but decreased by 47% in hippocampal mitochondria from EE rats, as compared with SE rats. Western blot analysis from nNOS protein expression associated to mitochondrial samples revealed a similar pattern. Our results suggest that in hippocampus and cerebral cortex, EE exposure protects mitochondria against calcium-induced MPT maintaining a convenient membrane potential, which assures a continuous energy supply.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151512
Lores Arnaiz, Silvia; Lores Arnaiz, Maria del Rosario; Czerniczyniec, Analia; Cuello, M; Bustamante, Joanita; Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment; Elsevier Science; Brain Research; 1319; 3-2010; 44-53
0006-8993
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151512
identifier_str_mv Lores Arnaiz, Silvia; Lores Arnaiz, Maria del Rosario; Czerniczyniec, Analia; Cuello, M; Bustamante, Joanita; Mitochondrial function and nitric oxide production in hippocampus and cerebral cortex of rats exposed to enriched environment; Elsevier Science; Brain Research; 1319; 3-2010; 44-53
0006-8993
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006899310000570
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainres.2010.01.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269840548560896
score 13.13397

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