Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals

Autores
Zulian, Sandra Edith; Ilincheta, Monica Graciela; Giusto, Norma Maria
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of the present study was to analyze diacylglycerol kinase (DAGK) activity in synaptic terminals from cerebral cortex (CC) and hippocampus (Hp) from adult (3-4 month-old) and aged (26-28 month-old) rats. The effect of insulin through DAGK activity on synaptosomes from adult and aged rats was also analyzed under conditions favoring saturated or unsaturated phosphatidic acid (PA) formation, using exogenous di-palmitoil glycerol (DPG) or 1-stearoyl-2-arachidonoylglycerol (SAG) as substrates. Results showed that the enzymatic activity preferentially uses 1-stearoyl-2-arachidonoylglycerol (SAG) as substrate, thus indicating the presence of -type DAGK. A significant decrease in DAGK activity transforming SAG into PA was also observed in both tissues from aged rats. Western Blot detection of DAGK showed that enzyme content undergoes no changes with aging. [3H]inositol incorporation into phosphoinosites was also analyzed to evaluate the role of DAGK in their synthesis. Data obtained from 3H-inositol incorporation into phosphoinositides revealed that in synaptosomes from aged rats phosphatidylinositol (PI) synthesis is lower than in adult animals. Interestingly, in the presence of SAG, PI synthesis was restored to adult values. DAGK activity over SAG was more highly stimulated by insulin in CC and Hp synaptosomes of aged rats with respect to adult rats. On the other hand, insulin exerted a stimulatory effect on PI and phosphatidylinositol 4 phosphate (PI(4)P) synthesis in synaptosomal CC from aged rats. Taken together, our findings indicate that in aged rats insulin triggers a stimulatory mechanism that reverts the diminished synaptosomal ability to synthesize arachidonoyl phosphatidic acid (20:4 PA). The recovery of this PA species indicates that insulin positively regulates phosphoinositide synthesis.
Fil: Zulian, Sandra Edith. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Ilincheta, Monica Graciela. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Materia
Aging
Dagk
Synaptic Terminals
Dag
Insulin
Cerebral Cortex
Hippocampus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7623
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7623
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminalsZulian, Sandra EdithIlincheta, Monica GracielaGiusto, Norma MariaAgingDagkSynaptic TerminalsDagInsulinCerebral CortexHippocampushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The purpose of the present study was to analyze diacylglycerol kinase (DAGK) activity in synaptic terminals from cerebral cortex (CC) and hippocampus (Hp) from adult (3-4 month-old) and aged (26-28 month-old) rats. The effect of insulin through DAGK activity on synaptosomes from adult and aged rats was also analyzed under conditions favoring saturated or unsaturated phosphatidic acid (PA) formation, using exogenous di-palmitoil glycerol (DPG) or 1-stearoyl-2-arachidonoylglycerol (SAG) as substrates. Results showed that the enzymatic activity preferentially uses 1-stearoyl-2-arachidonoylglycerol (SAG) as substrate, thus indicating the presence of -type DAGK. A significant decrease in DAGK activity transforming SAG into PA was also observed in both tissues from aged rats. Western Blot detection of DAGK showed that enzyme content undergoes no changes with aging. [3H]inositol incorporation into phosphoinosites was also analyzed to evaluate the role of DAGK in their synthesis. Data obtained from 3H-inositol incorporation into phosphoinositides revealed that in synaptosomes from aged rats phosphatidylinositol (PI) synthesis is lower than in adult animals. Interestingly, in the presence of SAG, PI synthesis was restored to adult values. DAGK activity over SAG was more highly stimulated by insulin in CC and Hp synaptosomes of aged rats with respect to adult rats. On the other hand, insulin exerted a stimulatory effect on PI and phosphatidylinositol 4 phosphate (PI(4)P) synthesis in synaptosomal CC from aged rats. Taken together, our findings indicate that in aged rats insulin triggers a stimulatory mechanism that reverts the diminished synaptosomal ability to synthesize arachidonoyl phosphatidic acid (20:4 PA). The recovery of this PA species indicates that insulin positively regulates phosphoinositide synthesis.Fil: Zulian, Sandra Edith. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Ilincheta, Monica Graciela. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaElsevier2010-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7623Zulian, Sandra Edith; Ilincheta, Monica Graciela; Giusto, Norma Maria; Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals; Elsevier; Neurochemistry International; 58; 3; 15-12-2010; 330-3360197-0186enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2010.12.005info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S019701861000375Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:52Zoai:ri.conicet.gov.ar:11336/7623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:52.426CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
title Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
spellingShingle Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
Zulian, Sandra Edith
Aging
Dagk
Synaptic Terminals
Dag
Insulin
Cerebral Cortex
Hippocampus
title_short Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
title_full Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
title_fullStr Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
title_full_unstemmed Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
title_sort Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals
dc.creator.none.fl_str_mv Zulian, Sandra Edith
Ilincheta, Monica Graciela
Giusto, Norma Maria
author Zulian, Sandra Edith
author_facet Zulian, Sandra Edith
Ilincheta, Monica Graciela
Giusto, Norma Maria
author_role author
author2 Ilincheta, Monica Graciela
Giusto, Norma Maria
author2_role author
author
dc.subject.none.fl_str_mv Aging
Dagk
Synaptic Terminals
Dag
Insulin
Cerebral Cortex
Hippocampus
topic Aging
Dagk
Synaptic Terminals
Dag
Insulin
Cerebral Cortex
Hippocampus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The purpose of the present study was to analyze diacylglycerol kinase (DAGK) activity in synaptic terminals from cerebral cortex (CC) and hippocampus (Hp) from adult (3-4 month-old) and aged (26-28 month-old) rats. The effect of insulin through DAGK activity on synaptosomes from adult and aged rats was also analyzed under conditions favoring saturated or unsaturated phosphatidic acid (PA) formation, using exogenous di-palmitoil glycerol (DPG) or 1-stearoyl-2-arachidonoylglycerol (SAG) as substrates. Results showed that the enzymatic activity preferentially uses 1-stearoyl-2-arachidonoylglycerol (SAG) as substrate, thus indicating the presence of -type DAGK. A significant decrease in DAGK activity transforming SAG into PA was also observed in both tissues from aged rats. Western Blot detection of DAGK showed that enzyme content undergoes no changes with aging. [3H]inositol incorporation into phosphoinosites was also analyzed to evaluate the role of DAGK in their synthesis. Data obtained from 3H-inositol incorporation into phosphoinositides revealed that in synaptosomes from aged rats phosphatidylinositol (PI) synthesis is lower than in adult animals. Interestingly, in the presence of SAG, PI synthesis was restored to adult values. DAGK activity over SAG was more highly stimulated by insulin in CC and Hp synaptosomes of aged rats with respect to adult rats. On the other hand, insulin exerted a stimulatory effect on PI and phosphatidylinositol 4 phosphate (PI(4)P) synthesis in synaptosomal CC from aged rats. Taken together, our findings indicate that in aged rats insulin triggers a stimulatory mechanism that reverts the diminished synaptosomal ability to synthesize arachidonoyl phosphatidic acid (20:4 PA). The recovery of this PA species indicates that insulin positively regulates phosphoinositide synthesis.
Fil: Zulian, Sandra Edith. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Ilincheta, Monica Graciela. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Cientificas y T__etilde__cnicas. Centro Cient__itilde__fico Tecnol__otilde__gico Bahia Blanca. Instituto de Investigaciones Bioqu__itilde__micas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
description The purpose of the present study was to analyze diacylglycerol kinase (DAGK) activity in synaptic terminals from cerebral cortex (CC) and hippocampus (Hp) from adult (3-4 month-old) and aged (26-28 month-old) rats. The effect of insulin through DAGK activity on synaptosomes from adult and aged rats was also analyzed under conditions favoring saturated or unsaturated phosphatidic acid (PA) formation, using exogenous di-palmitoil glycerol (DPG) or 1-stearoyl-2-arachidonoylglycerol (SAG) as substrates. Results showed that the enzymatic activity preferentially uses 1-stearoyl-2-arachidonoylglycerol (SAG) as substrate, thus indicating the presence of -type DAGK. A significant decrease in DAGK activity transforming SAG into PA was also observed in both tissues from aged rats. Western Blot detection of DAGK showed that enzyme content undergoes no changes with aging. [3H]inositol incorporation into phosphoinosites was also analyzed to evaluate the role of DAGK in their synthesis. Data obtained from 3H-inositol incorporation into phosphoinositides revealed that in synaptosomes from aged rats phosphatidylinositol (PI) synthesis is lower than in adult animals. Interestingly, in the presence of SAG, PI synthesis was restored to adult values. DAGK activity over SAG was more highly stimulated by insulin in CC and Hp synaptosomes of aged rats with respect to adult rats. On the other hand, insulin exerted a stimulatory effect on PI and phosphatidylinositol 4 phosphate (PI(4)P) synthesis in synaptosomal CC from aged rats. Taken together, our findings indicate that in aged rats insulin triggers a stimulatory mechanism that reverts the diminished synaptosomal ability to synthesize arachidonoyl phosphatidic acid (20:4 PA). The recovery of this PA species indicates that insulin positively regulates phosphoinositide synthesis.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7623
Zulian, Sandra Edith; Ilincheta, Monica Graciela; Giusto, Norma Maria; Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals; Elsevier; Neurochemistry International; 58; 3; 15-12-2010; 330-336
0197-0186
url http://hdl.handle.net/11336/7623
identifier_str_mv Zulian, Sandra Edith; Ilincheta, Monica Graciela; Giusto, Norma Maria; Insulin modifies aging-related inhibition of 1-stearoyl, 2-achidonoylglycerol phosphorylation in rat synaptic terminals; Elsevier; Neurochemistry International; 58; 3; 15-12-2010; 330-336
0197-0186
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2010.12.005
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S019701861000375X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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