Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock

Autores
Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; Peterson Maduro, Josi; Logister, Ive; Valentin, Guillaume; Morelli, Luis Guillermo; Oates, Andrew C.; Schulte Merker, Stefan
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
Fil: Lleras-Forero, Laura. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Narayanan, Rachna. The Francis Crick Institute; Reino Unido
Fil: Huitema, Leonie. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: VanBergen, Maaike. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Apschner, Alexander. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Peterson-Maduro, Josi. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Logister, Ive. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Valentin, Guillaume. The Francis Crick Institute; Reino Unido
Fil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Oates, Andrew C.. École Polytechnique Fédéral Laussane; Suiza
Fil: Schulte-Merker, Stefan. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Materia
NOTOCHORD
SOMITOGENESIS CLOCK
SOMITE
ENTPD5
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93783
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/93783
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clockLleras Forero, LauraNarayanan, RachnaHuitema, LeonieVan Bergen, MaaikeApschner, AlexanderPeterson Maduro, JosiLogister, IveValentin, GuillaumeMorelli, Luis GuillermoOates, Andrew C.Schulte Merker, StefanNOTOCHORDSOMITOGENESIS CLOCKSOMITEENTPD5https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.Fil: Lleras-Forero, Laura. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Narayanan, Rachna. The Francis Crick Institute; Reino UnidoFil: Huitema, Leonie. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: VanBergen, Maaike. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Apschner, Alexander. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Peterson-Maduro, Josi. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Logister, Ive. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Valentin, Guillaume. The Francis Crick Institute; Reino UnidoFil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Oates, Andrew C.. École Polytechnique Fédéral Laussane; SuizaFil: Schulte-Merker, Stefan. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaeLife Sciences Publications2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93783Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; et al.; Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock; eLife Sciences Publications; eLife; 7; 4-2018; 1-28; e338432050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/33843info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.33843info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:47:14Zoai:ri.conicet.gov.ar:11336/93783instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:47:14.542CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
spellingShingle Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
Lleras Forero, Laura
NOTOCHORD
SOMITOGENESIS CLOCK
SOMITE
ENTPD5
title_short Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_full Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_fullStr Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_full_unstemmed Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
title_sort Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
dc.creator.none.fl_str_mv Lleras Forero, Laura
Narayanan, Rachna
Huitema, Leonie
Van Bergen, Maaike
Apschner, Alexander
Peterson Maduro, Josi
Logister, Ive
Valentin, Guillaume
Morelli, Luis Guillermo
Oates, Andrew C.
Schulte Merker, Stefan
author Lleras Forero, Laura
author_facet Lleras Forero, Laura
Narayanan, Rachna
Huitema, Leonie
Van Bergen, Maaike
Apschner, Alexander
Peterson Maduro, Josi
Logister, Ive
Valentin, Guillaume
Morelli, Luis Guillermo
Oates, Andrew C.
Schulte Merker, Stefan
author_role author
author2 Narayanan, Rachna
Huitema, Leonie
Van Bergen, Maaike
Apschner, Alexander
Peterson Maduro, Josi
Logister, Ive
Valentin, Guillaume
Morelli, Luis Guillermo
Oates, Andrew C.
Schulte Merker, Stefan
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NOTOCHORD
SOMITOGENESIS CLOCK
SOMITE
ENTPD5
topic NOTOCHORD
SOMITOGENESIS CLOCK
SOMITE
ENTPD5
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
Fil: Lleras-Forero, Laura. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Narayanan, Rachna. The Francis Crick Institute; Reino Unido
Fil: Huitema, Leonie. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: VanBergen, Maaike. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Apschner, Alexander. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Peterson-Maduro, Josi. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Logister, Ive. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Valentin, Guillaume. The Francis Crick Institute; Reino Unido
Fil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Oates, Andrew C.. École Polytechnique Fédéral Laussane; Suiza
Fil: Schulte-Merker, Stefan. Institute For Cardiovascular Organogenesis And Regenera; Alemania
description Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93783
Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; et al.; Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock; eLife Sciences Publications; eLife; 7; 4-2018; 1-28; e33843
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93783
identifier_str_mv Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; et al.; Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock; eLife Sciences Publications; eLife; 7; 4-2018; 1-28; e33843
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/33843
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.33843
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782177536114688
score 12.982451

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