Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
- Autores
- Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; Peterson Maduro, Josi; Logister, Ive; Valentin, Guillaume; Morelli, Luis Guillermo; Oates, Andrew C.; Schulte Merker, Stefan
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.
Fil: Lleras-Forero, Laura. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Narayanan, Rachna. The Francis Crick Institute; Reino Unido
Fil: Huitema, Leonie. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: VanBergen, Maaike. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Apschner, Alexander. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Peterson-Maduro, Josi. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Logister, Ive. Institute For Cardiovascular Organogenesis And Regenera; Alemania
Fil: Valentin, Guillaume. The Francis Crick Institute; Reino Unido
Fil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Oates, Andrew C.. École Polytechnique Fédéral Laussane; Suiza
Fil: Schulte-Merker, Stefan. Institute For Cardiovascular Organogenesis And Regenera; Alemania - Materia
-
NOTOCHORD
SOMITOGENESIS CLOCK
SOMITE
ENTPD5 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/93783
Ver los metadatos del registro completo
id |
CONICETDig_e4c3ed041f6891bac33a6ef10a635b0c |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/93783 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clockLleras Forero, LauraNarayanan, RachnaHuitema, LeonieVan Bergen, MaaikeApschner, AlexanderPeterson Maduro, JosiLogister, IveValentin, GuillaumeMorelli, Luis GuillermoOates, Andrew C.Schulte Merker, StefanNOTOCHORDSOMITOGENESIS CLOCKSOMITEENTPD5https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.Fil: Lleras-Forero, Laura. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Narayanan, Rachna. The Francis Crick Institute; Reino UnidoFil: Huitema, Leonie. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: VanBergen, Maaike. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Apschner, Alexander. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Peterson-Maduro, Josi. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Logister, Ive. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaFil: Valentin, Guillaume. The Francis Crick Institute; Reino UnidoFil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Oates, Andrew C.. École Polytechnique Fédéral Laussane; SuizaFil: Schulte-Merker, Stefan. Institute For Cardiovascular Organogenesis And Regenera; AlemaniaeLife Sciences Publications2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93783Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; et al.; Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock; eLife Sciences Publications; eLife; 7; 4-2018; 1-28; e338432050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/33843info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.33843info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:05Zoai:ri.conicet.gov.ar:11336/93783instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:05.759CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
spellingShingle |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock Lleras Forero, Laura NOTOCHORD SOMITOGENESIS CLOCK SOMITE ENTPD5 |
title_short |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_full |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_fullStr |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_full_unstemmed |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
title_sort |
Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock |
dc.creator.none.fl_str_mv |
Lleras Forero, Laura Narayanan, Rachna Huitema, Leonie Van Bergen, Maaike Apschner, Alexander Peterson Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis Guillermo Oates, Andrew C. Schulte Merker, Stefan |
author |
Lleras Forero, Laura |
author_facet |
Lleras Forero, Laura Narayanan, Rachna Huitema, Leonie Van Bergen, Maaike Apschner, Alexander Peterson Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis Guillermo Oates, Andrew C. Schulte Merker, Stefan |
author_role |
author |
author2 |
Narayanan, Rachna Huitema, Leonie Van Bergen, Maaike Apschner, Alexander Peterson Maduro, Josi Logister, Ive Valentin, Guillaume Morelli, Luis Guillermo Oates, Andrew C. Schulte Merker, Stefan |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
NOTOCHORD SOMITOGENESIS CLOCK SOMITE ENTPD5 |
topic |
NOTOCHORD SOMITOGENESIS CLOCK SOMITE ENTPD5 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. Fil: Lleras-Forero, Laura. Institute For Cardiovascular Organogenesis And Regenera; Alemania Fil: Narayanan, Rachna. The Francis Crick Institute; Reino Unido Fil: Huitema, Leonie. Institute For Cardiovascular Organogenesis And Regenera; Alemania Fil: VanBergen, Maaike. Institute For Cardiovascular Organogenesis And Regenera; Alemania Fil: Apschner, Alexander. Institute For Cardiovascular Organogenesis And Regenera; Alemania Fil: Peterson-Maduro, Josi. Institute For Cardiovascular Organogenesis And Regenera; Alemania Fil: Logister, Ive. Institute For Cardiovascular Organogenesis And Regenera; Alemania Fil: Valentin, Guillaume. The Francis Crick Institute; Reino Unido Fil: Morelli, Luis Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina Fil: Oates, Andrew C.. École Polytechnique Fédéral Laussane; Suiza Fil: Schulte-Merker, Stefan. Institute For Cardiovascular Organogenesis And Regenera; Alemania |
description |
Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/93783 Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; et al.; Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock; eLife Sciences Publications; eLife; 7; 4-2018; 1-28; e33843 2050-084X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/93783 |
identifier_str_mv |
Lleras Forero, Laura; Narayanan, Rachna; Huitema, Leonie; Van Bergen, Maaike; Apschner, Alexander; et al.; Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock; eLife Sciences Publications; eLife; 7; 4-2018; 1-28; e33843 2050-084X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/33843 info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.33843 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
eLife Sciences Publications |
publisher.none.fl_str_mv |
eLife Sciences Publications |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614121027272704 |
score |
13.070432 |