Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis

Autores
Sanchez, Sara Serafina del V.; Sanchez, Romel Sebastian
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: In most vertebrates, the segmentation of the paraxial mesoderm involves the formation of metameric unitscalled somites through a mesenchymal-epithelial transition. However, this process is different in Xenopus laevis because itdoes not form an epithelial somite. Xenopus somitogenesis is characterized by a complex cells rearrangement that requiresthe coordinated regulation of cell shape, adhesion, and motility. The molecular mechanisms that control these cell behaviorsunderlying somite formation are little known. Although the Paraxis has been implicated in the epithelialization of somite inchick and mouse, its role in Xenopus somite morphogenesis has not been determined. Results: Using a morpholino andhormone-inducible construction approaches, we showed that both gain and loss of function of paraxis affect somite elongation,rotation and alignment, causing a severe disorganization of somitic tissue. We further found that depletion or overexpressionof paraxis in the somite led to the downregulation or upregulation, respectively, of cell adhesion expression markers.Finally, we demonstrated that paraxis is necessary for the proper expression of myotomal and sclerotomal differentiationmarkers. Conclusions: Our results demonstrate that paraxis regulates the cell rearrangements that take place during the somitogenesisof Xenopus by regulating cell adhesion. Furthermore, paraxis is also required for somite differentiation.
Fil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sanchez, Romel Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Xenopus
Somitogenesis
Paraxis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48190
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48190
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Paraxis is required for somite morphogenesis and differentiation in Xenopus laevisSanchez, Sara Serafina del V.Sanchez, Romel SebastianXenopusSomitogenesisParaxishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: In most vertebrates, the segmentation of the paraxial mesoderm involves the formation of metameric unitscalled somites through a mesenchymal-epithelial transition. However, this process is different in Xenopus laevis because itdoes not form an epithelial somite. Xenopus somitogenesis is characterized by a complex cells rearrangement that requiresthe coordinated regulation of cell shape, adhesion, and motility. The molecular mechanisms that control these cell behaviorsunderlying somite formation are little known. Although the Paraxis has been implicated in the epithelialization of somite inchick and mouse, its role in Xenopus somite morphogenesis has not been determined. Results: Using a morpholino andhormone-inducible construction approaches, we showed that both gain and loss of function of paraxis affect somite elongation,rotation and alignment, causing a severe disorganization of somitic tissue. We further found that depletion or overexpressionof paraxis in the somite led to the downregulation or upregulation, respectively, of cell adhesion expression markers.Finally, we demonstrated that paraxis is necessary for the proper expression of myotomal and sclerotomal differentiationmarkers. Conclusions: Our results demonstrate that paraxis regulates the cell rearrangements that take place during the somitogenesisof Xenopus by regulating cell adhesion. Furthermore, paraxis is also required for somite differentiation.Fil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Sanchez, Romel Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaWiley-liss, Div John Wiley & Sons Inc2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48190Sanchez, Sara Serafina del V.; Sanchez, Romel Sebastian; Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis; Wiley-liss, Div John Wiley & Sons Inc; Developmental Dynamics; 244; 8; 8-2015; 973-9871058-8388CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.24294info:eu-repo/semantics/altIdentifier/doi/10.1002/DVDY.24294info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:41:39Zoai:ri.conicet.gov.ar:11336/48190instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:41:39.997CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
title Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
spellingShingle Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
Sanchez, Sara Serafina del V.
Xenopus
Somitogenesis
Paraxis
title_short Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
title_full Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
title_fullStr Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
title_full_unstemmed Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
title_sort Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis
dc.creator.none.fl_str_mv Sanchez, Sara Serafina del V.
Sanchez, Romel Sebastian
author Sanchez, Sara Serafina del V.
author_facet Sanchez, Sara Serafina del V.
Sanchez, Romel Sebastian
author_role author
author2 Sanchez, Romel Sebastian
author2_role author
dc.subject.none.fl_str_mv Xenopus
Somitogenesis
Paraxis
topic Xenopus
Somitogenesis
Paraxis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: In most vertebrates, the segmentation of the paraxial mesoderm involves the formation of metameric unitscalled somites through a mesenchymal-epithelial transition. However, this process is different in Xenopus laevis because itdoes not form an epithelial somite. Xenopus somitogenesis is characterized by a complex cells rearrangement that requiresthe coordinated regulation of cell shape, adhesion, and motility. The molecular mechanisms that control these cell behaviorsunderlying somite formation are little known. Although the Paraxis has been implicated in the epithelialization of somite inchick and mouse, its role in Xenopus somite morphogenesis has not been determined. Results: Using a morpholino andhormone-inducible construction approaches, we showed that both gain and loss of function of paraxis affect somite elongation,rotation and alignment, causing a severe disorganization of somitic tissue. We further found that depletion or overexpressionof paraxis in the somite led to the downregulation or upregulation, respectively, of cell adhesion expression markers.Finally, we demonstrated that paraxis is necessary for the proper expression of myotomal and sclerotomal differentiationmarkers. Conclusions: Our results demonstrate that paraxis regulates the cell rearrangements that take place during the somitogenesisof Xenopus by regulating cell adhesion. Furthermore, paraxis is also required for somite differentiation.
Fil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sanchez, Romel Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Background: In most vertebrates, the segmentation of the paraxial mesoderm involves the formation of metameric unitscalled somites through a mesenchymal-epithelial transition. However, this process is different in Xenopus laevis because itdoes not form an epithelial somite. Xenopus somitogenesis is characterized by a complex cells rearrangement that requiresthe coordinated regulation of cell shape, adhesion, and motility. The molecular mechanisms that control these cell behaviorsunderlying somite formation are little known. Although the Paraxis has been implicated in the epithelialization of somite inchick and mouse, its role in Xenopus somite morphogenesis has not been determined. Results: Using a morpholino andhormone-inducible construction approaches, we showed that both gain and loss of function of paraxis affect somite elongation,rotation and alignment, causing a severe disorganization of somitic tissue. We further found that depletion or overexpressionof paraxis in the somite led to the downregulation or upregulation, respectively, of cell adhesion expression markers.Finally, we demonstrated that paraxis is necessary for the proper expression of myotomal and sclerotomal differentiationmarkers. Conclusions: Our results demonstrate that paraxis regulates the cell rearrangements that take place during the somitogenesisof Xenopus by regulating cell adhesion. Furthermore, paraxis is also required for somite differentiation.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48190
Sanchez, Sara Serafina del V.; Sanchez, Romel Sebastian; Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis; Wiley-liss, Div John Wiley & Sons Inc; Developmental Dynamics; 244; 8; 8-2015; 973-987
1058-8388
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48190
identifier_str_mv Sanchez, Sara Serafina del V.; Sanchez, Romel Sebastian; Paraxis is required for somite morphogenesis and differentiation in Xenopus laevis; Wiley-liss, Div John Wiley & Sons Inc; Developmental Dynamics; 244; 8; 8-2015; 973-987
1058-8388
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/dvdy.24294
info:eu-repo/semantics/altIdentifier/doi/10.1002/DVDY.24294
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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