Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children

Autores
Rodríguez, Fátima; Salinas, Claudia; Fernández, Silvina; Haim, Maria Sol; Mollerach, Marta Eugenia; Basualdo, Wilma; Castro, Héctor; Quiñónez, Beatriz; Arguello, Rocío; Rodríguez, Mónica; Grau, Lorena; Espínola, Carmen; Velázquez, Gladys; Samudio, Gloria; Gómez, Gloria; Campuzano, Ana; Ortellado, Juana; Almada, Patricia; Guillén, Rosa
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan children patients in two periods of time. Methodology: An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. Results: The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. Conclusions: This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay.
Fil: Rodríguez, Fátima. Universidad Nacional de Asunción; Paraguay
Fil: Salinas, Claudia. Universidad Nacional de Asunción; Paraguay
Fil: Fernández, Silvina. Universidad de Buenos Aires; Argentina
Fil: Haim, Maria Sol. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basualdo, Wilma. Hospital Central Instituto de Previsiín Social; Paraguay. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Castro, Héctor. Hospital Central Instituto de Previsiín Social; Paraguay. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Quiñónez, Beatriz. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Arguello, Rocío. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Rodríguez, Mónica. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Grau, Lorena. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Espínola, Carmen. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Velázquez, Gladys. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Samudio, Gloria. Hospital Nacional, Panama; Panamá
Fil: Gómez, Gloria. Hospital Nacional, Panama; Panamá
Fil: Campuzano, Ana. Hospital de Clínicas; Paraguay
Fil: Ortellado, Juana. Hospital de Clínicas; Paraguay
Fil: Almada, Patricia. Hospital de Clínicas; Paraguay
Fil: Guillén, Rosa. Universidad Nacional de Asunción; Paraguay
Materia
CHILDREN
COMMUNITY-ASSOCIATED
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
MRSA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170518
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/170518
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan childrenRodríguez, FátimaSalinas, ClaudiaFernández, SilvinaHaim, Maria SolMollerach, Marta EugeniaBasualdo, WilmaCastro, HéctorQuiñónez, BeatrizArguello, RocíoRodríguez, MónicaGrau, LorenaEspínola, CarmenVelázquez, GladysSamudio, GloriaGómez, GloriaCampuzano, AnaOrtellado, JuanaAlmada, PatriciaGuillén, RosaCHILDRENCOMMUNITY-ASSOCIATEDMETHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUSMRSAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Introduction: Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan children patients in two periods of time. Methodology: An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. Results: The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. Conclusions: This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay.Fil: Rodríguez, Fátima. Universidad Nacional de Asunción; ParaguayFil: Salinas, Claudia. Universidad Nacional de Asunción; ParaguayFil: Fernández, Silvina. Universidad de Buenos Aires; ArgentinaFil: Haim, Maria Sol. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mollerach, Marta Eugenia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Basualdo, Wilma. Hospital Central Instituto de Previsiín Social; Paraguay. Ministerio de Salud Pública y Bienestar Social; ParaguayFil: Castro, Héctor. Hospital Central Instituto de Previsiín Social; Paraguay. Ministerio de Salud Pública y Bienestar Social; ParaguayFil: Quiñónez, Beatriz. Ministerio de Salud Pública y Bienestar Social; ParaguayFil: Arguello, Rocío. Ministerio de Salud Pública y Bienestar Social; ParaguayFil: Rodríguez, Mónica. Hospital Central Instituto de Previsiín Social; ParaguayFil: Grau, Lorena. Hospital Central Instituto de Previsiín Social; ParaguayFil: Espínola, Carmen. Hospital Central Instituto de Previsiín Social; ParaguayFil: Velázquez, Gladys. Hospital Central Instituto de Previsiín Social; ParaguayFil: Samudio, Gloria. Hospital Nacional, Panama; PanamáFil: Gómez, Gloria. Hospital Nacional, Panama; PanamáFil: Campuzano, Ana. Hospital de Clínicas; ParaguayFil: Ortellado, Juana. Hospital de Clínicas; ParaguayFil: Almada, Patricia. Hospital de Clínicas; ParaguayFil: Guillén, Rosa. Universidad Nacional de Asunción; ParaguayJournal of Infection in Developing Countries2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170518Rodríguez, Fátima; Salinas, Claudia; Fernández, Silvina; Haim, Maria Sol; Mollerach, Marta Eugenia; et al.; Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 14; 3; 3-2020; 290-2971972-2680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3855/jidc.12108info:eu-repo/semantics/altIdentifier/url/https://www.jidc.org/index.php/journal/article/view/12108info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:38Zoai:ri.conicet.gov.ar:11336/170518instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:39.023CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
title Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
spellingShingle Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
Rodríguez, Fátima
CHILDREN
COMMUNITY-ASSOCIATED
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
MRSA
title_short Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
title_full Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
title_fullStr Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
title_full_unstemmed Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
title_sort Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children
dc.creator.none.fl_str_mv Rodríguez, Fátima
Salinas, Claudia
Fernández, Silvina
Haim, Maria Sol
Mollerach, Marta Eugenia
Basualdo, Wilma
Castro, Héctor
Quiñónez, Beatriz
Arguello, Rocío
Rodríguez, Mónica
Grau, Lorena
Espínola, Carmen
Velázquez, Gladys
Samudio, Gloria
Gómez, Gloria
Campuzano, Ana
Ortellado, Juana
Almada, Patricia
Guillén, Rosa
author Rodríguez, Fátima
author_facet Rodríguez, Fátima
Salinas, Claudia
Fernández, Silvina
Haim, Maria Sol
Mollerach, Marta Eugenia
Basualdo, Wilma
Castro, Héctor
Quiñónez, Beatriz
Arguello, Rocío
Rodríguez, Mónica
Grau, Lorena
Espínola, Carmen
Velázquez, Gladys
Samudio, Gloria
Gómez, Gloria
Campuzano, Ana
Ortellado, Juana
Almada, Patricia
Guillén, Rosa
author_role author
author2 Salinas, Claudia
Fernández, Silvina
Haim, Maria Sol
Mollerach, Marta Eugenia
Basualdo, Wilma
Castro, Héctor
Quiñónez, Beatriz
Arguello, Rocío
Rodríguez, Mónica
Grau, Lorena
Espínola, Carmen
Velázquez, Gladys
Samudio, Gloria
Gómez, Gloria
Campuzano, Ana
Ortellado, Juana
Almada, Patricia
Guillén, Rosa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHILDREN
COMMUNITY-ASSOCIATED
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
MRSA
topic CHILDREN
COMMUNITY-ASSOCIATED
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
MRSA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan children patients in two periods of time. Methodology: An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. Results: The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. Conclusions: This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay.
Fil: Rodríguez, Fátima. Universidad Nacional de Asunción; Paraguay
Fil: Salinas, Claudia. Universidad Nacional de Asunción; Paraguay
Fil: Fernández, Silvina. Universidad de Buenos Aires; Argentina
Fil: Haim, Maria Sol. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basualdo, Wilma. Hospital Central Instituto de Previsiín Social; Paraguay. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Castro, Héctor. Hospital Central Instituto de Previsiín Social; Paraguay. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Quiñónez, Beatriz. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Arguello, Rocío. Ministerio de Salud Pública y Bienestar Social; Paraguay
Fil: Rodríguez, Mónica. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Grau, Lorena. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Espínola, Carmen. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Velázquez, Gladys. Hospital Central Instituto de Previsiín Social; Paraguay
Fil: Samudio, Gloria. Hospital Nacional, Panama; Panamá
Fil: Gómez, Gloria. Hospital Nacional, Panama; Panamá
Fil: Campuzano, Ana. Hospital de Clínicas; Paraguay
Fil: Ortellado, Juana. Hospital de Clínicas; Paraguay
Fil: Almada, Patricia. Hospital de Clínicas; Paraguay
Fil: Guillén, Rosa. Universidad Nacional de Asunción; Paraguay
description Introduction: Staphylococcus aureus is considered one of the most important human pathogens, and its levels of resistance to methicillin have increased even in strains isolated from people without nosocomial risk factors. Molecular analysis is essential for understanding the patterns of dissemination. The objective of this study was to identify community-acquired methicillin-resistant S. aureus (CA-MRSA) clones that infected Paraguayan children patients in two periods of time. Methodology: An observational, descriptive study was designed to determine the genetic variability of 115 isolates of CA-MRSA recovered from children who attended four reference centers in Paraguay between 2009-2010 and 2012-2013. Results: The combined use of Pulsed Field Gel Electrophoresis (PFGE), Multi-Locus Sequencing Typing, Multi-Locus Variable Analysis (MLVA) and Spa typing techniques allowed the identification of two dominant clones: ST30-IV-t019 (77%) and ST5-IV-t311 (10%), and the establishment of the former as the leading cause of CA-MRSA infections in children during the study period. Conclusions: This is the first study that provides epidemiological information as well as microbiological and molecular characteristics of CA-MRSA isolates recovered from children from Asunción and the Central Department of Paraguay.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170518
Rodríguez, Fátima; Salinas, Claudia; Fernández, Silvina; Haim, Maria Sol; Mollerach, Marta Eugenia; et al.; Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 14; 3; 3-2020; 290-297
1972-2680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170518
identifier_str_mv Rodríguez, Fátima; Salinas, Claudia; Fernández, Silvina; Haim, Maria Sol; Mollerach, Marta Eugenia; et al.; Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones from Paraguayan children; Journal of Infection in Developing Countries; Journal of Infection in Developing Countries; 14; 3; 3-2020; 290-297
1972-2680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3855/jidc.12108
info:eu-repo/semantics/altIdentifier/url/https://www.jidc.org/index.php/journal/article/view/12108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Infection in Developing Countries
publisher.none.fl_str_mv Journal of Infection in Developing Countries
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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