Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina

Autores
Moreno Mochi, María Paula; Vargas, Juan Martin; Vivaldo, S.; Bottiglieri, M.; López, C.; Mochi, S.; Cobos, M.; Castillo, M.; del Campo, R.; Jure, Maria Angela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: In Latin America, methicillin-resistantStaphylococcus aureus (MRSA) is a leading cause of nosocomial infections. Limited studies have addressed the molecular epidemiology of MRSA clones in Argentina, characterised by continuous human migratory movements. The aim of this study was to describe the MRSA epidemiology, including distinct patient populations from different regions of the country. Methods: MRSA strains were collected in epidemiological studies conducted from 2009 to 2015 in three cities (Formosa, Córdoba and Tucumán) and involving four population groups: community adult patients; hospitalised adults; hospitalised children; and healthy children (nasal colonisation). Antimicrobial susceptibility testing, SCCmec and Panton–Valentine leukocidin (PVL) typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed. Results: A total of 120 MRSA isolates were recovered with an important population diversity in the groups studied; in community adult patients, MRSA isolates corresponded to ST5, ST267 and ST1619; from hospitalised adults they were ST97, ST5, ST72, ST125, ST200, ST647, ST747, ST935 and ST2941; from hospitalised children they were ST5, ST30, ST34, ST1163 and ST1619; and from colonised children they were ST5, ST125, ST34, ST100, ST1619, ST207 and ST1163. Results of SCCmec typing showed SCCmec I, SCCmec IIIA, SCCmec IV and SCCmec ND associated or not with PVL genes. Conclusions: MRSA genetic lineages have differing distribution in the three regions. The most prevalent was ST5 in colonisation, community and invasive settings. Here we describe ST34-SCCmec IV clone for the first time in the hospitalised paediatric population. These findings contribute to the understanding of epidemiological changes in recent years.
Fil: Moreno Mochi, María Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vargas, Juan Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Vivaldo, S.. Hospital de la Madre y El Niño; Argentina
Fil: Bottiglieri, M.. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: López, C.. Centro de Microbiología Médica; Argentina
Fil: Mochi, S.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: Cobos, M.. Hospital Ramon y Cajal; España. Instituto Ramón y Cajal de Investigaciones Sanitarias; España
Fil: Castillo, M.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: del Campo, R.. Instituto Ramón y Cajal de Investigaciones Sanitarias; España. Hospital Ramon y Cajal; España
Fil: Jure, Maria Angela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Materia
METHICILLIN-RESISTANT
MOLECULAR EPIDEMIOLOGY
MRSA
POPULATION GROUPS
STAPHYLOCOCCUS AUREUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218444
Acceder
id CONICETDig_ecceb45f5a2635815724158ddb7baab2
oai_identifier_str oai:ri.conicet.gov.ar:11336/218444
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in ArgentinaMoreno Mochi, María PaulaVargas, Juan MartinVivaldo, S.Bottiglieri, M.López, C.Mochi, S.Cobos, M.Castillo, M.del Campo, R.Jure, Maria AngelaMETHICILLIN-RESISTANTMOLECULAR EPIDEMIOLOGYMRSAPOPULATION GROUPSSTAPHYLOCOCCUS AUREUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objectives: In Latin America, methicillin-resistantStaphylococcus aureus (MRSA) is a leading cause of nosocomial infections. Limited studies have addressed the molecular epidemiology of MRSA clones in Argentina, characterised by continuous human migratory movements. The aim of this study was to describe the MRSA epidemiology, including distinct patient populations from different regions of the country. Methods: MRSA strains were collected in epidemiological studies conducted from 2009 to 2015 in three cities (Formosa, Córdoba and Tucumán) and involving four population groups: community adult patients; hospitalised adults; hospitalised children; and healthy children (nasal colonisation). Antimicrobial susceptibility testing, SCCmec and Panton–Valentine leukocidin (PVL) typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed. Results: A total of 120 MRSA isolates were recovered with an important population diversity in the groups studied; in community adult patients, MRSA isolates corresponded to ST5, ST267 and ST1619; from hospitalised adults they were ST97, ST5, ST72, ST125, ST200, ST647, ST747, ST935 and ST2941; from hospitalised children they were ST5, ST30, ST34, ST1163 and ST1619; and from colonised children they were ST5, ST125, ST34, ST100, ST1619, ST207 and ST1163. Results of SCCmec typing showed SCCmec I, SCCmec IIIA, SCCmec IV and SCCmec ND associated or not with PVL genes. Conclusions: MRSA genetic lineages have differing distribution in the three regions. The most prevalent was ST5 in colonisation, community and invasive settings. Here we describe ST34-SCCmec IV clone for the first time in the hospitalised paediatric population. These findings contribute to the understanding of epidemiological changes in recent years.Fil: Moreno Mochi, María Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vargas, Juan Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Vivaldo, S.. Hospital de la Madre y El Niño; ArgentinaFil: Bottiglieri, M.. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: López, C.. Centro de Microbiología Médica; ArgentinaFil: Mochi, S.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; ArgentinaFil: Cobos, M.. Hospital Ramon y Cajal; España. Instituto Ramón y Cajal de Investigaciones Sanitarias; EspañaFil: Castillo, M.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: del Campo, R.. Instituto Ramón y Cajal de Investigaciones Sanitarias; España. Hospital Ramon y Cajal; EspañaFil: Jure, Maria Angela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaElsevier Ltd2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218444Moreno Mochi, María Paula; Vargas, Juan Martin; Vivaldo, S.; Bottiglieri, M.; López, C.; et al.; Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina; Elsevier Ltd; Journal of Global Antimicrobial Resistance; 23; 12-2020; 82-862213-71652213-7173CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jgar.2020.07.016info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2213716520301910info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:42Zoai:ri.conicet.gov.ar:11336/218444instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:42.838CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
title Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
spellingShingle Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
Moreno Mochi, María Paula
METHICILLIN-RESISTANT
MOLECULAR EPIDEMIOLOGY
MRSA
POPULATION GROUPS
STAPHYLOCOCCUS AUREUS
title_short Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
title_full Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
title_fullStr Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
title_full_unstemmed Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
title_sort Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
dc.creator.none.fl_str_mv Moreno Mochi, María Paula
Vargas, Juan Martin
Vivaldo, S.
Bottiglieri, M.
López, C.
Mochi, S.
Cobos, M.
Castillo, M.
del Campo, R.
Jure, Maria Angela
author Moreno Mochi, María Paula
author_facet Moreno Mochi, María Paula
Vargas, Juan Martin
Vivaldo, S.
Bottiglieri, M.
López, C.
Mochi, S.
Cobos, M.
Castillo, M.
del Campo, R.
Jure, Maria Angela
author_role author
author2 Vargas, Juan Martin
Vivaldo, S.
Bottiglieri, M.
López, C.
Mochi, S.
Cobos, M.
Castillo, M.
del Campo, R.
Jure, Maria Angela
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv METHICILLIN-RESISTANT
MOLECULAR EPIDEMIOLOGY
MRSA
POPULATION GROUPS
STAPHYLOCOCCUS AUREUS
topic METHICILLIN-RESISTANT
MOLECULAR EPIDEMIOLOGY
MRSA
POPULATION GROUPS
STAPHYLOCOCCUS AUREUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objectives: In Latin America, methicillin-resistantStaphylococcus aureus (MRSA) is a leading cause of nosocomial infections. Limited studies have addressed the molecular epidemiology of MRSA clones in Argentina, characterised by continuous human migratory movements. The aim of this study was to describe the MRSA epidemiology, including distinct patient populations from different regions of the country. Methods: MRSA strains were collected in epidemiological studies conducted from 2009 to 2015 in three cities (Formosa, Córdoba and Tucumán) and involving four population groups: community adult patients; hospitalised adults; hospitalised children; and healthy children (nasal colonisation). Antimicrobial susceptibility testing, SCCmec and Panton–Valentine leukocidin (PVL) typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed. Results: A total of 120 MRSA isolates were recovered with an important population diversity in the groups studied; in community adult patients, MRSA isolates corresponded to ST5, ST267 and ST1619; from hospitalised adults they were ST97, ST5, ST72, ST125, ST200, ST647, ST747, ST935 and ST2941; from hospitalised children they were ST5, ST30, ST34, ST1163 and ST1619; and from colonised children they were ST5, ST125, ST34, ST100, ST1619, ST207 and ST1163. Results of SCCmec typing showed SCCmec I, SCCmec IIIA, SCCmec IV and SCCmec ND associated or not with PVL genes. Conclusions: MRSA genetic lineages have differing distribution in the three regions. The most prevalent was ST5 in colonisation, community and invasive settings. Here we describe ST34-SCCmec IV clone for the first time in the hospitalised paediatric population. These findings contribute to the understanding of epidemiological changes in recent years.
Fil: Moreno Mochi, María Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vargas, Juan Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Vivaldo, S.. Hospital de la Madre y El Niño; Argentina
Fil: Bottiglieri, M.. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: López, C.. Centro de Microbiología Médica; Argentina
Fil: Mochi, S.. Gobierno de la Provincia de Tucumán. Hospital Ángel Padilla; Argentina
Fil: Cobos, M.. Hospital Ramon y Cajal; España. Instituto Ramón y Cajal de Investigaciones Sanitarias; España
Fil: Castillo, M.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: del Campo, R.. Instituto Ramón y Cajal de Investigaciones Sanitarias; España. Hospital Ramon y Cajal; España
Fil: Jure, Maria Angela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
description Objectives: In Latin America, methicillin-resistantStaphylococcus aureus (MRSA) is a leading cause of nosocomial infections. Limited studies have addressed the molecular epidemiology of MRSA clones in Argentina, characterised by continuous human migratory movements. The aim of this study was to describe the MRSA epidemiology, including distinct patient populations from different regions of the country. Methods: MRSA strains were collected in epidemiological studies conducted from 2009 to 2015 in three cities (Formosa, Córdoba and Tucumán) and involving four population groups: community adult patients; hospitalised adults; hospitalised children; and healthy children (nasal colonisation). Antimicrobial susceptibility testing, SCCmec and Panton–Valentine leukocidin (PVL) typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed. Results: A total of 120 MRSA isolates were recovered with an important population diversity in the groups studied; in community adult patients, MRSA isolates corresponded to ST5, ST267 and ST1619; from hospitalised adults they were ST97, ST5, ST72, ST125, ST200, ST647, ST747, ST935 and ST2941; from hospitalised children they were ST5, ST30, ST34, ST1163 and ST1619; and from colonised children they were ST5, ST125, ST34, ST100, ST1619, ST207 and ST1163. Results of SCCmec typing showed SCCmec I, SCCmec IIIA, SCCmec IV and SCCmec ND associated or not with PVL genes. Conclusions: MRSA genetic lineages have differing distribution in the three regions. The most prevalent was ST5 in colonisation, community and invasive settings. Here we describe ST34-SCCmec IV clone for the first time in the hospitalised paediatric population. These findings contribute to the understanding of epidemiological changes in recent years.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218444
Moreno Mochi, María Paula; Vargas, Juan Martin; Vivaldo, S.; Bottiglieri, M.; López, C.; et al.; Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina; Elsevier Ltd; Journal of Global Antimicrobial Resistance; 23; 12-2020; 82-86
2213-7165
2213-7173
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218444
identifier_str_mv Moreno Mochi, María Paula; Vargas, Juan Martin; Vivaldo, S.; Bottiglieri, M.; López, C.; et al.; Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina; Elsevier Ltd; Journal of Global Antimicrobial Resistance; 23; 12-2020; 82-86
2213-7165
2213-7173
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jgar.2020.07.016
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2213716520301910
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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