Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol

Autores
Yunes, Roberto Miguel Federico; Estrella, Cecilia R.; García Menéndez, Sebastián Marcelo Manuel; Lara, Hernán E.; Cabrera Kreiker, Ricardo Jorge
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ethanol consumption during pregnancy may induce profound changes in fetal CNS development. We postulate that some of the effects of ethanol on striatal glutamatergic transmission and neurotrophin expression could be modulated by allopregnanolone, a neurosteroid modulator of GABA A receptor activity. We describe the acute pharmacological effect of allopregnanolone (65 μg/kg, s.c.) administered to juvenile male rats (day 21 of age) on the corticostriatal glutamatergic pathway, in both control and prenatally ethanol-exposed rats (two ip injections of 2.9 g/kg in 24% v/v saline solution on gestational day 8). Prenatal ethanol administration decreased the K+-induced release of glutamate regarding the control group. Interestingly, this effect was reverted by allopregnanolone. Regarding BDNF, allopregnanolone decreases the content of this neurotrophic factor in the striatum of control groups. However, both ethanol alone and ethanol plus allopregnanolone treated animals did not show any change regarding control values. We suggest that prenatal ethanol exposure may produce an alteration of GABA A receptors which blocks the GABA agonist-like effect of allopregnanolone on rapid glutamate release, thus disturbing normal neural transmission. Furthermore, the reciprocal interactions found between GABAergic neurosteroids and BDNF could underlie mechanisms operating during the neuronal plasticity of fetal development.
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas; Argentina
Fil: Estrella, Cecilia R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: García Menéndez, Sebastián Marcelo Manuel. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Lara, Hernán E.. Universidad de Chile; Chile
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
ALLOPREGNANOLONA
GLUTAMATE
BDNF
RAT
PRENATALLY
ETHANOL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128686
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128686
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanolYunes, Roberto Miguel FedericoEstrella, Cecilia R.García Menéndez, Sebastián Marcelo ManuelLara, Hernán E.Cabrera Kreiker, Ricardo JorgeALLOPREGNANOLONAGLUTAMATEBDNFRATPRENATALLYETHANOLhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Ethanol consumption during pregnancy may induce profound changes in fetal CNS development. We postulate that some of the effects of ethanol on striatal glutamatergic transmission and neurotrophin expression could be modulated by allopregnanolone, a neurosteroid modulator of GABA A receptor activity. We describe the acute pharmacological effect of allopregnanolone (65 μg/kg, s.c.) administered to juvenile male rats (day 21 of age) on the corticostriatal glutamatergic pathway, in both control and prenatally ethanol-exposed rats (two ip injections of 2.9 g/kg in 24% v/v saline solution on gestational day 8). Prenatal ethanol administration decreased the K+-induced release of glutamate regarding the control group. Interestingly, this effect was reverted by allopregnanolone. Regarding BDNF, allopregnanolone decreases the content of this neurotrophic factor in the striatum of control groups. However, both ethanol alone and ethanol plus allopregnanolone treated animals did not show any change regarding control values. We suggest that prenatal ethanol exposure may produce an alteration of GABA A receptors which blocks the GABA agonist-like effect of allopregnanolone on rapid glutamate release, thus disturbing normal neural transmission. Furthermore, the reciprocal interactions found between GABAergic neurosteroids and BDNF could underlie mechanisms operating during the neuronal plasticity of fetal development.Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas; ArgentinaFil: Estrella, Cecilia R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: García Menéndez, Sebastián Marcelo Manuel. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Lara, Hernán E.. Universidad de Chile; ChileFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaHindawi Publishing Corporation2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128686Yunes, Roberto Miguel Federico; Estrella, Cecilia R.; García Menéndez, Sebastián Marcelo Manuel; Lara, Hernán E.; Cabrera Kreiker, Ricardo Jorge; Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol; Hindawi Publishing Corporation; BioMed Research International; 2015; 734367; 2-2015; 1-62314-61332314-6141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2015/734367info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2015/734367/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:08Zoai:ri.conicet.gov.ar:11336/128686instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:08.663CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
title Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
spellingShingle Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
Yunes, Roberto Miguel Federico
ALLOPREGNANOLONA
GLUTAMATE
BDNF
RAT
PRENATALLY
ETHANOL
title_short Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
title_full Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
title_fullStr Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
title_full_unstemmed Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
title_sort Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol
dc.creator.none.fl_str_mv Yunes, Roberto Miguel Federico
Estrella, Cecilia R.
García Menéndez, Sebastián Marcelo Manuel
Lara, Hernán E.
Cabrera Kreiker, Ricardo Jorge
author Yunes, Roberto Miguel Federico
author_facet Yunes, Roberto Miguel Federico
Estrella, Cecilia R.
García Menéndez, Sebastián Marcelo Manuel
Lara, Hernán E.
Cabrera Kreiker, Ricardo Jorge
author_role author
author2 Estrella, Cecilia R.
García Menéndez, Sebastián Marcelo Manuel
Lara, Hernán E.
Cabrera Kreiker, Ricardo Jorge
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALLOPREGNANOLONA
GLUTAMATE
BDNF
RAT
PRENATALLY
ETHANOL
topic ALLOPREGNANOLONA
GLUTAMATE
BDNF
RAT
PRENATALLY
ETHANOL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ethanol consumption during pregnancy may induce profound changes in fetal CNS development. We postulate that some of the effects of ethanol on striatal glutamatergic transmission and neurotrophin expression could be modulated by allopregnanolone, a neurosteroid modulator of GABA A receptor activity. We describe the acute pharmacological effect of allopregnanolone (65 μg/kg, s.c.) administered to juvenile male rats (day 21 of age) on the corticostriatal glutamatergic pathway, in both control and prenatally ethanol-exposed rats (two ip injections of 2.9 g/kg in 24% v/v saline solution on gestational day 8). Prenatal ethanol administration decreased the K+-induced release of glutamate regarding the control group. Interestingly, this effect was reverted by allopregnanolone. Regarding BDNF, allopregnanolone decreases the content of this neurotrophic factor in the striatum of control groups. However, both ethanol alone and ethanol plus allopregnanolone treated animals did not show any change regarding control values. We suggest that prenatal ethanol exposure may produce an alteration of GABA A receptors which blocks the GABA agonist-like effect of allopregnanolone on rapid glutamate release, thus disturbing normal neural transmission. Furthermore, the reciprocal interactions found between GABAergic neurosteroids and BDNF could underlie mechanisms operating during the neuronal plasticity of fetal development.
Fil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas; Argentina
Fil: Estrella, Cecilia R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: García Menéndez, Sebastián Marcelo Manuel. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Lara, Hernán E.. Universidad de Chile; Chile
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Ethanol consumption during pregnancy may induce profound changes in fetal CNS development. We postulate that some of the effects of ethanol on striatal glutamatergic transmission and neurotrophin expression could be modulated by allopregnanolone, a neurosteroid modulator of GABA A receptor activity. We describe the acute pharmacological effect of allopregnanolone (65 μg/kg, s.c.) administered to juvenile male rats (day 21 of age) on the corticostriatal glutamatergic pathway, in both control and prenatally ethanol-exposed rats (two ip injections of 2.9 g/kg in 24% v/v saline solution on gestational day 8). Prenatal ethanol administration decreased the K+-induced release of glutamate regarding the control group. Interestingly, this effect was reverted by allopregnanolone. Regarding BDNF, allopregnanolone decreases the content of this neurotrophic factor in the striatum of control groups. However, both ethanol alone and ethanol plus allopregnanolone treated animals did not show any change regarding control values. We suggest that prenatal ethanol exposure may produce an alteration of GABA A receptors which blocks the GABA agonist-like effect of allopregnanolone on rapid glutamate release, thus disturbing normal neural transmission. Furthermore, the reciprocal interactions found between GABAergic neurosteroids and BDNF could underlie mechanisms operating during the neuronal plasticity of fetal development.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128686
Yunes, Roberto Miguel Federico; Estrella, Cecilia R.; García Menéndez, Sebastián Marcelo Manuel; Lara, Hernán E.; Cabrera Kreiker, Ricardo Jorge; Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol; Hindawi Publishing Corporation; BioMed Research International; 2015; 734367; 2-2015; 1-6
2314-6133
2314-6141
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128686
identifier_str_mv Yunes, Roberto Miguel Federico; Estrella, Cecilia R.; García Menéndez, Sebastián Marcelo Manuel; Lara, Hernán E.; Cabrera Kreiker, Ricardo Jorge; Postnatal administration of allopregnanolone modifies glutamate release but Not BDNF content in striatum samples of rats prenatally exposed to ethanol; Hindawi Publishing Corporation; BioMed Research International; 2015; 734367; 2-2015; 1-6
2314-6133
2314-6141
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2015/734367
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2015/734367/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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