Glutamate neurotransmission is affected in prenatally stressed offspring

Autores
Adrover, Ezequiela; Pallares, Maria Eugenia; Baier, Carlos Javier; Monteleone, Melisa Carolina; Giuliani, Fernando Alfredo; Waagepetersen, Helle S.; Brocco, Marcela Adriana; Cabrera, Ricardo José; Sonnewald, Ursula; Schousboe, Arne; Antonelli, Marta Cristina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies from our laboratory have shown that male adult offspring of stressed mothers exhibited higher levels of ionotropic and metabotropic glutamate receptors than control rats. These offspring also showed long-lasting astroglial hypertrophy and a reduced dendritic arborization with synaptic loss. Since metabolism of glutamate is dependent on interactions between neurons and surrounding astroglia, our results suggest that glutamate neurotransmitter pathways might be impaired in the brain of prenatally stressed rats. To study the effect of prenatal stress on the metabolism and neurotransmitter function of glutamate, pregnant rats were subjected to restrain stress during the last week of gestation. Brains of the adult offspring were used to assess glutamate metabolism, uptake and release as well as expression of glutamate receptors and transporters. While glutamate metabolism was not affected it was found that prenatal stress (PS) changed the expression of the transporters, thus, producing a higher level of vesicular vGluT-1 in the frontal cortex (FCx) and elevated levels of GLT1 protein and messenger RNA in the hippocampus (HPC) of adult male PS offspring. We also observed increased uptake capacity for glutamate in the FCx of PS male offspring while no such changes were observed in the HPC. The results show that changes mediated by PS on the adult glutamatergic system are brain region specific. Overall, PS produces long-term changes in the glutamatergic system modulating the expression of glutamate transporters and altering synaptic transmission of the adult brain.
Fil: Adrover, Ezequiela. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina
Fil: Pallares, Maria Eugenia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina
Fil: Baier, Carlos Javier. Universidad de Buenos Aires; Argentina
Fil: Monteleone, Melisa Carolina. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Waagepetersen, Helle S.. Universidad de Copenhagen; Dinamarca
Fil: Brocco, Marcela Adriana. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
Fil: Cabrera, Ricardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sonnewald, Ursula. Norwegian University of Science and Technology; Noruega
Fil: Schousboe, Arne. Universidad de Copenhagen; Dinamarca
Fil: Antonelli, Marta Cristina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina
Materia
Glutamate Release
Glutamate Transporters
Nmda Receptor
Ampa Receptor
Hippocampus
Frontal Cortex
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4375
Acceder
id CONICETDig_26eb18e79b4a9bab6b1041eb19db14a2
oai_identifier_str oai:ri.conicet.gov.ar:11336/4375
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glutamate neurotransmission is affected in prenatally stressed offspringAdrover, EzequielaPallares, Maria EugeniaBaier, Carlos JavierMonteleone, Melisa CarolinaGiuliani, Fernando AlfredoWaagepetersen, Helle S.Brocco, Marcela AdrianaCabrera, Ricardo JoséSonnewald, UrsulaSchousboe, ArneAntonelli, Marta CristinaGlutamate ReleaseGlutamate TransportersNmda ReceptorAmpa ReceptorHippocampusFrontal Cortexhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Previous studies from our laboratory have shown that male adult offspring of stressed mothers exhibited higher levels of ionotropic and metabotropic glutamate receptors than control rats. These offspring also showed long-lasting astroglial hypertrophy and a reduced dendritic arborization with synaptic loss. Since metabolism of glutamate is dependent on interactions between neurons and surrounding astroglia, our results suggest that glutamate neurotransmitter pathways might be impaired in the brain of prenatally stressed rats. To study the effect of prenatal stress on the metabolism and neurotransmitter function of glutamate, pregnant rats were subjected to restrain stress during the last week of gestation. Brains of the adult offspring were used to assess glutamate metabolism, uptake and release as well as expression of glutamate receptors and transporters. While glutamate metabolism was not affected it was found that prenatal stress (PS) changed the expression of the transporters, thus, producing a higher level of vesicular vGluT-1 in the frontal cortex (FCx) and elevated levels of GLT1 protein and messenger RNA in the hippocampus (HPC) of adult male PS offspring. We also observed increased uptake capacity for glutamate in the FCx of PS male offspring while no such changes were observed in the HPC. The results show that changes mediated by PS on the adult glutamatergic system are brain region specific. Overall, PS produces long-term changes in the glutamatergic system modulating the expression of glutamate transporters and altering synaptic transmission of the adult brain.Fil: Adrover, Ezequiela. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; ArgentinaFil: Pallares, Maria Eugenia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; ArgentinaFil: Baier, Carlos Javier. Universidad de Buenos Aires; ArgentinaFil: Monteleone, Melisa Carolina. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); ArgentinaFil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Waagepetersen, Helle S.. Universidad de Copenhagen; DinamarcaFil: Brocco, Marcela Adriana. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); ArgentinaFil: Cabrera, Ricardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Sonnewald, Ursula. Norwegian University of Science and Technology; NoruegaFil: Schousboe, Arne. Universidad de Copenhagen; DinamarcaFil: Antonelli, Marta Cristina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; ArgentinaPergamon-elsevier Science Ltd2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4375Adrover, Ezequiela; Pallares, Maria Eugenia; Baier, Carlos Javier; Monteleone, Melisa Carolina; Giuliani, Fernando Alfredo; et al.; Glutamate neurotransmission is affected in prenatally stressed offspring; Pergamon-elsevier Science Ltd; Neurochemistry International; 88; 5-2015; 73-870197-0186enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2015.05.005info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0197018615000832info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:13Zoai:ri.conicet.gov.ar:11336/4375instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:14.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glutamate neurotransmission is affected in prenatally stressed offspring
title Glutamate neurotransmission is affected in prenatally stressed offspring
spellingShingle Glutamate neurotransmission is affected in prenatally stressed offspring
Adrover, Ezequiela
Glutamate Release
Glutamate Transporters
Nmda Receptor
Ampa Receptor
Hippocampus
Frontal Cortex
title_short Glutamate neurotransmission is affected in prenatally stressed offspring
title_full Glutamate neurotransmission is affected in prenatally stressed offspring
title_fullStr Glutamate neurotransmission is affected in prenatally stressed offspring
title_full_unstemmed Glutamate neurotransmission is affected in prenatally stressed offspring
title_sort Glutamate neurotransmission is affected in prenatally stressed offspring
dc.creator.none.fl_str_mv Adrover, Ezequiela
Pallares, Maria Eugenia
Baier, Carlos Javier
Monteleone, Melisa Carolina
Giuliani, Fernando Alfredo
Waagepetersen, Helle S.
Brocco, Marcela Adriana
Cabrera, Ricardo José
Sonnewald, Ursula
Schousboe, Arne
Antonelli, Marta Cristina
author Adrover, Ezequiela
author_facet Adrover, Ezequiela
Pallares, Maria Eugenia
Baier, Carlos Javier
Monteleone, Melisa Carolina
Giuliani, Fernando Alfredo
Waagepetersen, Helle S.
Brocco, Marcela Adriana
Cabrera, Ricardo José
Sonnewald, Ursula
Schousboe, Arne
Antonelli, Marta Cristina
author_role author
author2 Pallares, Maria Eugenia
Baier, Carlos Javier
Monteleone, Melisa Carolina
Giuliani, Fernando Alfredo
Waagepetersen, Helle S.
Brocco, Marcela Adriana
Cabrera, Ricardo José
Sonnewald, Ursula
Schousboe, Arne
Antonelli, Marta Cristina
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Glutamate Release
Glutamate Transporters
Nmda Receptor
Ampa Receptor
Hippocampus
Frontal Cortex
topic Glutamate Release
Glutamate Transporters
Nmda Receptor
Ampa Receptor
Hippocampus
Frontal Cortex
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Previous studies from our laboratory have shown that male adult offspring of stressed mothers exhibited higher levels of ionotropic and metabotropic glutamate receptors than control rats. These offspring also showed long-lasting astroglial hypertrophy and a reduced dendritic arborization with synaptic loss. Since metabolism of glutamate is dependent on interactions between neurons and surrounding astroglia, our results suggest that glutamate neurotransmitter pathways might be impaired in the brain of prenatally stressed rats. To study the effect of prenatal stress on the metabolism and neurotransmitter function of glutamate, pregnant rats were subjected to restrain stress during the last week of gestation. Brains of the adult offspring were used to assess glutamate metabolism, uptake and release as well as expression of glutamate receptors and transporters. While glutamate metabolism was not affected it was found that prenatal stress (PS) changed the expression of the transporters, thus, producing a higher level of vesicular vGluT-1 in the frontal cortex (FCx) and elevated levels of GLT1 protein and messenger RNA in the hippocampus (HPC) of adult male PS offspring. We also observed increased uptake capacity for glutamate in the FCx of PS male offspring while no such changes were observed in the HPC. The results show that changes mediated by PS on the adult glutamatergic system are brain region specific. Overall, PS produces long-term changes in the glutamatergic system modulating the expression of glutamate transporters and altering synaptic transmission of the adult brain.
Fil: Adrover, Ezequiela. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina
Fil: Pallares, Maria Eugenia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina
Fil: Baier, Carlos Javier. Universidad de Buenos Aires; Argentina
Fil: Monteleone, Melisa Carolina. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
Fil: Giuliani, Fernando Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Waagepetersen, Helle S.. Universidad de Copenhagen; Dinamarca
Fil: Brocco, Marcela Adriana. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
Fil: Cabrera, Ricardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sonnewald, Ursula. Norwegian University of Science and Technology; Noruega
Fil: Schousboe, Arne. Universidad de Copenhagen; Dinamarca
Fil: Antonelli, Marta Cristina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "Profesor Eduardo de Robertis"; Argentina
description Previous studies from our laboratory have shown that male adult offspring of stressed mothers exhibited higher levels of ionotropic and metabotropic glutamate receptors than control rats. These offspring also showed long-lasting astroglial hypertrophy and a reduced dendritic arborization with synaptic loss. Since metabolism of glutamate is dependent on interactions between neurons and surrounding astroglia, our results suggest that glutamate neurotransmitter pathways might be impaired in the brain of prenatally stressed rats. To study the effect of prenatal stress on the metabolism and neurotransmitter function of glutamate, pregnant rats were subjected to restrain stress during the last week of gestation. Brains of the adult offspring were used to assess glutamate metabolism, uptake and release as well as expression of glutamate receptors and transporters. While glutamate metabolism was not affected it was found that prenatal stress (PS) changed the expression of the transporters, thus, producing a higher level of vesicular vGluT-1 in the frontal cortex (FCx) and elevated levels of GLT1 protein and messenger RNA in the hippocampus (HPC) of adult male PS offspring. We also observed increased uptake capacity for glutamate in the FCx of PS male offspring while no such changes were observed in the HPC. The results show that changes mediated by PS on the adult glutamatergic system are brain region specific. Overall, PS produces long-term changes in the glutamatergic system modulating the expression of glutamate transporters and altering synaptic transmission of the adult brain.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4375
Adrover, Ezequiela; Pallares, Maria Eugenia; Baier, Carlos Javier; Monteleone, Melisa Carolina; Giuliani, Fernando Alfredo; et al.; Glutamate neurotransmission is affected in prenatally stressed offspring; Pergamon-elsevier Science Ltd; Neurochemistry International; 88; 5-2015; 73-87
0197-0186
url http://hdl.handle.net/11336/4375
identifier_str_mv Adrover, Ezequiela; Pallares, Maria Eugenia; Baier, Carlos Javier; Monteleone, Melisa Carolina; Giuliani, Fernando Alfredo; et al.; Glutamate neurotransmission is affected in prenatally stressed offspring; Pergamon-elsevier Science Ltd; Neurochemistry International; 88; 5-2015; 73-87
0197-0186
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2015.05.005
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0197018615000832
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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