The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
- Autores
- Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating.
Fil: Curtin, Paul C. P.. City University Of New York; Estados Unidos
Fil: Medan, Violeta. City University Of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neumeister, Heike. City University Of New York; Estados Unidos
Fil: Bronson, Daniel R.. City University Of New York; Estados Unidos
Fil: Preuss, Thomas. City University Of New York; Estados Unidos - Materia
-
Mauthner
Startle
Serotonin Receptor 5a
Prepulse Inhibition - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/20745
Ver los metadatos del registro completo
id |
CONICETDig_e11d89a3ced427c2845f5d8dbd9318e6 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/20745 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gatingCurtin, Paul C. P.Medan, VioletaNeumeister, HeikeBronson, Daniel R.Preuss, ThomasMauthnerStartleSerotonin Receptor 5aPrepulse Inhibitionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating.Fil: Curtin, Paul C. P.. City University Of New York; Estados UnidosFil: Medan, Violeta. City University Of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Neumeister, Heike. City University Of New York; Estados UnidosFil: Bronson, Daniel R.. City University Of New York; Estados UnidosFil: Preuss, Thomas. City University Of New York; Estados UnidosSociety for Neuroscience2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20745Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas; The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating; Society for Neuroscience; Journal of Neuroscience; 33; 24; 6-2013; 10011-100200270-6474CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4733-12.2013info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/33/24/10011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:59Zoai:ri.conicet.gov.ar:11336/20745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:59.753CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating |
title |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating |
spellingShingle |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating Curtin, Paul C. P. Mauthner Startle Serotonin Receptor 5a Prepulse Inhibition |
title_short |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating |
title_full |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating |
title_fullStr |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating |
title_full_unstemmed |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating |
title_sort |
The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating |
dc.creator.none.fl_str_mv |
Curtin, Paul C. P. Medan, Violeta Neumeister, Heike Bronson, Daniel R. Preuss, Thomas |
author |
Curtin, Paul C. P. |
author_facet |
Curtin, Paul C. P. Medan, Violeta Neumeister, Heike Bronson, Daniel R. Preuss, Thomas |
author_role |
author |
author2 |
Medan, Violeta Neumeister, Heike Bronson, Daniel R. Preuss, Thomas |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Mauthner Startle Serotonin Receptor 5a Prepulse Inhibition |
topic |
Mauthner Startle Serotonin Receptor 5a Prepulse Inhibition |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating. Fil: Curtin, Paul C. P.. City University Of New York; Estados Unidos Fil: Medan, Violeta. City University Of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Neumeister, Heike. City University Of New York; Estados Unidos Fil: Bronson, Daniel R.. City University Of New York; Estados Unidos Fil: Preuss, Thomas. City University Of New York; Estados Unidos |
description |
Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/20745 Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas; The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating; Society for Neuroscience; Journal of Neuroscience; 33; 24; 6-2013; 10011-10020 0270-6474 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/20745 |
identifier_str_mv |
Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas; The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating; Society for Neuroscience; Journal of Neuroscience; 33; 24; 6-2013; 10011-10020 0270-6474 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4733-12.2013 info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/33/24/10011 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for Neuroscience |
publisher.none.fl_str_mv |
Society for Neuroscience |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614100401782784 |
score |
13.070432 |