The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating

Autores
Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating.
Fil: Curtin, Paul C. P.. City University Of New York; Estados Unidos
Fil: Medan, Violeta. City University Of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neumeister, Heike. City University Of New York; Estados Unidos
Fil: Bronson, Daniel R.. City University Of New York; Estados Unidos
Fil: Preuss, Thomas. City University Of New York; Estados Unidos
Materia
Mauthner
Startle
Serotonin Receptor 5a
Prepulse Inhibition
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20745

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network_name_str CONICET Digital (CONICET)
spelling The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gatingCurtin, Paul C. P.Medan, VioletaNeumeister, HeikeBronson, Daniel R.Preuss, ThomasMauthnerStartleSerotonin Receptor 5aPrepulse Inhibitionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating.Fil: Curtin, Paul C. P.. City University Of New York; Estados UnidosFil: Medan, Violeta. City University Of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Neumeister, Heike. City University Of New York; Estados UnidosFil: Bronson, Daniel R.. City University Of New York; Estados UnidosFil: Preuss, Thomas. City University Of New York; Estados UnidosSociety for Neuroscience2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20745Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas; The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating; Society for Neuroscience; Journal of Neuroscience; 33; 24; 6-2013; 10011-100200270-6474CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4733-12.2013info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/33/24/10011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:59Zoai:ri.conicet.gov.ar:11336/20745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:59.753CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
title The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
spellingShingle The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
Curtin, Paul C. P.
Mauthner
Startle
Serotonin Receptor 5a
Prepulse Inhibition
title_short The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
title_full The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
title_fullStr The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
title_full_unstemmed The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
title_sort The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating
dc.creator.none.fl_str_mv Curtin, Paul C. P.
Medan, Violeta
Neumeister, Heike
Bronson, Daniel R.
Preuss, Thomas
author Curtin, Paul C. P.
author_facet Curtin, Paul C. P.
Medan, Violeta
Neumeister, Heike
Bronson, Daniel R.
Preuss, Thomas
author_role author
author2 Medan, Violeta
Neumeister, Heike
Bronson, Daniel R.
Preuss, Thomas
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mauthner
Startle
Serotonin Receptor 5a
Prepulse Inhibition
topic Mauthner
Startle
Serotonin Receptor 5a
Prepulse Inhibition
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating.
Fil: Curtin, Paul C. P.. City University Of New York; Estados Unidos
Fil: Medan, Violeta. City University Of New York; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neumeister, Heike. City University Of New York; Estados Unidos
Fil: Bronson, Daniel R.. City University Of New York; Estados Unidos
Fil: Preuss, Thomas. City University Of New York; Estados Unidos
description Here we applied behavioral testing, pharmacology, and in vivo electrophysiology to determine the function of the serotonin 5-HT5A receptor in goldfish startle plasticity and sensorimotor gating. In an initial series of behavioral experiments, we characterized the effects of a selective 5-HT5A antagonist, SB-699551 (3-cyclopentyl-N-[2-(dimethylamino)ethyl]-N-[(4 -{[(2-phenylethyl)amino]methyl}-4- biphenylyl)methyl]propanamide dihydrochloride), on prepulse inhibition of the acoustic startle response. Those experiments showed a dose-dependent decline in startle rates in prepulse conditions. Subsequent behavioral experiments showed that SB-699551 also reduced baseline startle rates (i.e., without prepulse). To determine the cellular mechanisms underlying these behaviors, we tested the effects of two distinct selective 5-HT5A antagonists, SB-699551 and A-843277 (N-(2,6-dimethoxybenzyl)-N [4-(4-fluorophenyl)thiazol- 2-yl]guanidine), on the intrinsic membrane properties and synaptic sound response of the Mauthner cell (M-cell), the decision-making neuron of the startle circuit. Auditory-evoked postsynaptic potentials recorded in the M-cell were similarly attenuated after treatment with either 5-HT5A antagonist (SB-699551, 26.413.98% reduction; A-843277, 17.526.24% reduction). This attenuation was produced by a tonic (intrinsic) reduction in M-cell input resistance, likely mediated by a Cl conductance, that added to the extrinsic inhibition produced by an auditory prepulse. Interestingly,the effector mechanisms underlying neural prepulse inhibition itself were unaffected by antagonist treatment. In summary, these results provide an in vivo electrophysiological characterization of the 5-HT5A receptor and its behavioral relevance and provide a new perspective on the interaction of intrinsic and extrinsic modulatory mechanisms in startle plasticity and sensorimotor gating.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20745
Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas; The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating; Society for Neuroscience; Journal of Neuroscience; 33; 24; 6-2013; 10011-10020
0270-6474
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20745
identifier_str_mv Curtin, Paul C. P.; Medan, Violeta; Neumeister, Heike; Bronson, Daniel R.; Preuss, Thomas; The 5-HT5A receptor regulates excitability in the auditory startle circuit: functional implications for sensorimotor gating; Society for Neuroscience; Journal of Neuroscience; 33; 24; 6-2013; 10011-10020
0270-6474
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4733-12.2013
info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/33/24/10011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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