Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis

Autores
Joray, Mariana Belén; Trucco, Lucas Daniel; González, María Laura; Diaz Napal, Georgina Natalia; Palacios, Sara Maria; Bocco, Jose Luis; Carpinella, Maria Cecilia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1), isoliquiritigenin (2), pinocembrin (3), 7-hydroxyflavanone (4), and 7,4′-dihydroxy-3′-methoxyflavanone (5). Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC) values ranging from 31 to 62 and 62 to 250 g/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1-5 was determined by MTT assay on acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) cell lines including their multidrug resistant (MDR) phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6-9.9 M) and a lower effect against CML cells (IC50 = 27.5-30.0 M). Flow cytometry was used to analyze cell cycle distribution and cell death by PI-labeled cells and by Annexin V/PI staining, respectively. Upon treatment, 1 induced cell cycle arrest in the G2/M phase accompanied by a strong induction of apoptosis. These results describe for the first time the antibacterial metabolites of F. oolepis extract, with 1 being the most effective. This chalcone also emerges as a selective cytotoxic agent against sensitive and resistant leukemic cells, highlighting its potential as a lead compound.
Fil: Joray, Mariana Belén. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trucco, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: González, María Laura. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Diaz Napal, Georgina Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacios, Sara Maria. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
PLANTAS NATIVAS
CITOTOXICOS NATURALES
ANTIBACTERIANOS NATURALES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/70488
Acceder
id CONICETDig_debd36dd2bbd38e2c5a9deb647f37861
oai_identifier_str oai:ri.conicet.gov.ar:11336/70488
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepisJoray, Mariana BelénTrucco, Lucas DanielGonzález, María LauraDiaz Napal, Georgina NataliaPalacios, Sara MariaBocco, Jose LuisCarpinella, Maria CeciliaPLANTAS NATIVASCITOTOXICOS NATURALESANTIBACTERIANOS NATURALEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1), isoliquiritigenin (2), pinocembrin (3), 7-hydroxyflavanone (4), and 7,4′-dihydroxy-3′-methoxyflavanone (5). Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC) values ranging from 31 to 62 and 62 to 250 g/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1-5 was determined by MTT assay on acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) cell lines including their multidrug resistant (MDR) phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6-9.9 M) and a lower effect against CML cells (IC50 = 27.5-30.0 M). Flow cytometry was used to analyze cell cycle distribution and cell death by PI-labeled cells and by Annexin V/PI staining, respectively. Upon treatment, 1 induced cell cycle arrest in the G2/M phase accompanied by a strong induction of apoptosis. These results describe for the first time the antibacterial metabolites of F. oolepis extract, with 1 being the most effective. This chalcone also emerges as a selective cytotoxic agent against sensitive and resistant leukemic cells, highlighting its potential as a lead compound.Fil: Joray, Mariana Belén. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trucco, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: González, María Laura. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Diaz Napal, Georgina Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palacios, Sara Maria. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaHindawi Publishing Corporation2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/70488Joray, Mariana Belén; Trucco, Lucas Daniel; González, María Laura; Diaz Napal, Georgina Natalia; Palacios, Sara Maria; et al.; Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis; Hindawi Publishing Corporation; Evidence-Based Complementary and Alternative Medicine; 2015; 12-2015; 1-11; 9124841741-427X1741-4288CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ecam/2015/912484/info:eu-repo/semantics/altIdentifier/doi/10.1155/2015/912484info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:52:26Zoai:ri.conicet.gov.ar:11336/70488instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:52:26.526CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
title Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
spellingShingle Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
Joray, Mariana Belén
PLANTAS NATIVAS
CITOTOXICOS NATURALES
ANTIBACTERIANOS NATURALES
title_short Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
title_full Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
title_fullStr Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
title_full_unstemmed Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
title_sort Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis
dc.creator.none.fl_str_mv Joray, Mariana Belén
Trucco, Lucas Daniel
González, María Laura
Diaz Napal, Georgina Natalia
Palacios, Sara Maria
Bocco, Jose Luis
Carpinella, Maria Cecilia
author Joray, Mariana Belén
author_facet Joray, Mariana Belén
Trucco, Lucas Daniel
González, María Laura
Diaz Napal, Georgina Natalia
Palacios, Sara Maria
Bocco, Jose Luis
Carpinella, Maria Cecilia
author_role author
author2 Trucco, Lucas Daniel
González, María Laura
Diaz Napal, Georgina Natalia
Palacios, Sara Maria
Bocco, Jose Luis
Carpinella, Maria Cecilia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PLANTAS NATIVAS
CITOTOXICOS NATURALES
ANTIBACTERIANOS NATURALES
topic PLANTAS NATIVAS
CITOTOXICOS NATURALES
ANTIBACTERIANOS NATURALES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1), isoliquiritigenin (2), pinocembrin (3), 7-hydroxyflavanone (4), and 7,4′-dihydroxy-3′-methoxyflavanone (5). Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC) values ranging from 31 to 62 and 62 to 250 g/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1-5 was determined by MTT assay on acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) cell lines including their multidrug resistant (MDR) phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6-9.9 M) and a lower effect against CML cells (IC50 = 27.5-30.0 M). Flow cytometry was used to analyze cell cycle distribution and cell death by PI-labeled cells and by Annexin V/PI staining, respectively. Upon treatment, 1 induced cell cycle arrest in the G2/M phase accompanied by a strong induction of apoptosis. These results describe for the first time the antibacterial metabolites of F. oolepis extract, with 1 being the most effective. This chalcone also emerges as a selective cytotoxic agent against sensitive and resistant leukemic cells, highlighting its potential as a lead compound.
Fil: Joray, Mariana Belén. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trucco, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: González, María Laura. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Diaz Napal, Georgina Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palacios, Sara Maria. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1), isoliquiritigenin (2), pinocembrin (3), 7-hydroxyflavanone (4), and 7,4′-dihydroxy-3′-methoxyflavanone (5). Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC) values ranging from 31 to 62 and 62 to 250 g/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1-5 was determined by MTT assay on acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) cell lines including their multidrug resistant (MDR) phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6-9.9 M) and a lower effect against CML cells (IC50 = 27.5-30.0 M). Flow cytometry was used to analyze cell cycle distribution and cell death by PI-labeled cells and by Annexin V/PI staining, respectively. Upon treatment, 1 induced cell cycle arrest in the G2/M phase accompanied by a strong induction of apoptosis. These results describe for the first time the antibacterial metabolites of F. oolepis extract, with 1 being the most effective. This chalcone also emerges as a selective cytotoxic agent against sensitive and resistant leukemic cells, highlighting its potential as a lead compound.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/70488
Joray, Mariana Belén; Trucco, Lucas Daniel; González, María Laura; Diaz Napal, Georgina Natalia; Palacios, Sara Maria; et al.; Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis; Hindawi Publishing Corporation; Evidence-Based Complementary and Alternative Medicine; 2015; 12-2015; 1-11; 912484
1741-427X
1741-4288
CONICET Digital
CONICET
url http://hdl.handle.net/11336/70488
identifier_str_mv Joray, Mariana Belén; Trucco, Lucas Daniel; González, María Laura; Diaz Napal, Georgina Natalia; Palacios, Sara Maria; et al.; Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis; Hindawi Publishing Corporation; Evidence-Based Complementary and Alternative Medicine; 2015; 12-2015; 1-11; 912484
1741-427X
1741-4288
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ecam/2015/912484/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2015/912484
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847977262322810880
score 13.087074

Publicaciones similares