An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine

Autores
Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Labbé, Pierre; Solis, Velia Matilde
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A biosensor based on the immobilization of Lactate oxidase in laponite - organosilasesquioxane films on glassy carbon electrode for the quantification of l-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.33 ± 0.01) A cm-2 M-1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Labbé, Pierre. Centre National de la Recherche Scientifique; Francia
Fil: Solis, Velia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
BIOSENSORS
DAIRY PRODUCTS
ENZYMES
FERROCENE-METHANOL
L-LACTATE
LACTATE OXIDASE BIOSENSOR
LAPONITE GEL
WINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/188286
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/188286
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red winePaz Zanini, Veronica IreneLopez, Beatriz AliciaLabbé, PierreSolis, Velia MatildeBIOSENSORSDAIRY PRODUCTSENZYMESFERROCENE-METHANOLL-LACTATELACTATE OXIDASE BIOSENSORLAPONITE GELWINEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A biosensor based on the immobilization of Lactate oxidase in laponite - organosilasesquioxane films on glassy carbon electrode for the quantification of l-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.33 ± 0.01) A cm-2 M-1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods.Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Labbé, Pierre. Centre National de la Recherche Scientifique; FranciaFil: Solis, Velia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaWiley VCH Verlag2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/188286Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Labbé, Pierre; Solis, Velia Matilde; An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine; Wiley VCH Verlag; Electroanalysis; 22; 9; 5-2010; 946-9541040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/elan.200900416info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200900416info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:43:51Zoai:ri.conicet.gov.ar:11336/188286instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:43:51.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
title An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
spellingShingle An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
Paz Zanini, Veronica Irene
BIOSENSORS
DAIRY PRODUCTS
ENZYMES
FERROCENE-METHANOL
L-LACTATE
LACTATE OXIDASE BIOSENSOR
LAPONITE GEL
WINE
title_short An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
title_full An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
title_fullStr An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
title_full_unstemmed An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
title_sort An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine
dc.creator.none.fl_str_mv Paz Zanini, Veronica Irene
Lopez, Beatriz Alicia
Labbé, Pierre
Solis, Velia Matilde
author Paz Zanini, Veronica Irene
author_facet Paz Zanini, Veronica Irene
Lopez, Beatriz Alicia
Labbé, Pierre
Solis, Velia Matilde
author_role author
author2 Lopez, Beatriz Alicia
Labbé, Pierre
Solis, Velia Matilde
author2_role author
author
author
dc.subject.none.fl_str_mv BIOSENSORS
DAIRY PRODUCTS
ENZYMES
FERROCENE-METHANOL
L-LACTATE
LACTATE OXIDASE BIOSENSOR
LAPONITE GEL
WINE
topic BIOSENSORS
DAIRY PRODUCTS
ENZYMES
FERROCENE-METHANOL
L-LACTATE
LACTATE OXIDASE BIOSENSOR
LAPONITE GEL
WINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A biosensor based on the immobilization of Lactate oxidase in laponite - organosilasesquioxane films on glassy carbon electrode for the quantification of l-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.33 ± 0.01) A cm-2 M-1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Labbé, Pierre. Centre National de la Recherche Scientifique; Francia
Fil: Solis, Velia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description A biosensor based on the immobilization of Lactate oxidase in laponite - organosilasesquioxane films on glassy carbon electrode for the quantification of l-lactate in wine and dairy products is presented. The bioelectrode showed a very high sensitivity (0.33 ± 0.01) A cm-2 M-1 and a short time response (10 s) for less than 1 U of enzyme. No significant interferences, including ascorbic acid, were detected. For red wine, matrix effects assigned to polyphenols and anthocyanins were observed, which ware easily overcome by sample dilution. Our l-lactate determinations were in good agreement with those of two standard methods.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/188286
Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Labbé, Pierre; Solis, Velia Matilde; An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine; Wiley VCH Verlag; Electroanalysis; 22; 9; 5-2010; 946-954
1040-0397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/188286
identifier_str_mv Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Labbé, Pierre; Solis, Velia Matilde; An L-lactate amperometric enzyme electrode based on L-lactate oxidase immobilized in a laponite gel on a glassy carbon electrode. Application to dairy products and red wine; Wiley VCH Verlag; Electroanalysis; 22; 9; 5-2010; 946-954
1040-0397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/elan.200900416
info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200900416
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082946072576000
score 13.22299

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