Mathematical modeling and experimental results of a sandwich-type amperometric biosensor

Autores
Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A comprehensive numerical treatment of the diffusion and reaction within a sandwich-type amperometric biosensor is presented. The model considers that the enzyme reacts according to a ping-pong mechanism and that it is entrapped into a so-called enzymatic matrix placed between two diffusion membranes. It is found that the concentrations of reagents and products within the sensor are difficult of being compared to those of the bulk. In this regard, the use of approximate analytical solutions would involve errors in the analysis of kinetic parameters corresponding to this kind of biosensors. Provided the mediator species are in high concentration or diffuse much faster than the substrate, the response time of a biosensor of this kind would be determined by the diffusion of the substrate though the external membrane. In this sense, those systems with immobilized mediators, in which diffusion of electrons or holes is assumed for the charge transport process, could be also described by this model. Thus, the thickness and the permeability to the analyte of the external membrane are critical parameters for improving the response time of a sandwich-type biosensor. The simulated curves are compared with experimental profiles corresponding to a lactate amperometric biosensor obtaining consistent results. In a future publication a non-linear fitting algorithm will be combined to the model for the extraction of kinetic and/or geometric parameters.
Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Biosensors
Polymers
Lactate
Simulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20600
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mathematical modeling and experimental results of a sandwich-type amperometric biosensorRomero, Marcelo RicardoBaruzzi, Ana MariaGaray, Fernando SebastianBiosensorsPolymersLactateSimulationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A comprehensive numerical treatment of the diffusion and reaction within a sandwich-type amperometric biosensor is presented. The model considers that the enzyme reacts according to a ping-pong mechanism and that it is entrapped into a so-called enzymatic matrix placed between two diffusion membranes. It is found that the concentrations of reagents and products within the sensor are difficult of being compared to those of the bulk. In this regard, the use of approximate analytical solutions would involve errors in the analysis of kinetic parameters corresponding to this kind of biosensors. Provided the mediator species are in high concentration or diffuse much faster than the substrate, the response time of a biosensor of this kind would be determined by the diffusion of the substrate though the external membrane. In this sense, those systems with immobilized mediators, in which diffusion of electrons or holes is assumed for the charge transport process, could be also described by this model. Thus, the thickness and the permeability to the analyte of the external membrane are critical parameters for improving the response time of a sandwich-type biosensor. The simulated curves are compared with experimental profiles corresponding to a lactate amperometric biosensor obtaining consistent results. In a future publication a non-linear fitting algorithm will be combined to the model for the extraction of kinetic and/or geometric parameters.Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20600Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian; Mathematical modeling and experimental results of a sandwich-type amperometric biosensor; Elsevier Science; Sensors and Actuators B: Chemical; 162; 1; 12-2011; 284-2910925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.12.079info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400511011695info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:06:41Zoai:ri.conicet.gov.ar:11336/20600instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:06:42.006CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
title Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
spellingShingle Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
Romero, Marcelo Ricardo
Biosensors
Polymers
Lactate
Simulation
title_short Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
title_full Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
title_fullStr Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
title_full_unstemmed Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
title_sort Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
dc.creator.none.fl_str_mv Romero, Marcelo Ricardo
Baruzzi, Ana Maria
Garay, Fernando Sebastian
author Romero, Marcelo Ricardo
author_facet Romero, Marcelo Ricardo
Baruzzi, Ana Maria
Garay, Fernando Sebastian
author_role author
author2 Baruzzi, Ana Maria
Garay, Fernando Sebastian
author2_role author
author
dc.subject.none.fl_str_mv Biosensors
Polymers
Lactate
Simulation
topic Biosensors
Polymers
Lactate
Simulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A comprehensive numerical treatment of the diffusion and reaction within a sandwich-type amperometric biosensor is presented. The model considers that the enzyme reacts according to a ping-pong mechanism and that it is entrapped into a so-called enzymatic matrix placed between two diffusion membranes. It is found that the concentrations of reagents and products within the sensor are difficult of being compared to those of the bulk. In this regard, the use of approximate analytical solutions would involve errors in the analysis of kinetic parameters corresponding to this kind of biosensors. Provided the mediator species are in high concentration or diffuse much faster than the substrate, the response time of a biosensor of this kind would be determined by the diffusion of the substrate though the external membrane. In this sense, those systems with immobilized mediators, in which diffusion of electrons or holes is assumed for the charge transport process, could be also described by this model. Thus, the thickness and the permeability to the analyte of the external membrane are critical parameters for improving the response time of a sandwich-type biosensor. The simulated curves are compared with experimental profiles corresponding to a lactate amperometric biosensor obtaining consistent results. In a future publication a non-linear fitting algorithm will be combined to the model for the extraction of kinetic and/or geometric parameters.
Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description A comprehensive numerical treatment of the diffusion and reaction within a sandwich-type amperometric biosensor is presented. The model considers that the enzyme reacts according to a ping-pong mechanism and that it is entrapped into a so-called enzymatic matrix placed between two diffusion membranes. It is found that the concentrations of reagents and products within the sensor are difficult of being compared to those of the bulk. In this regard, the use of approximate analytical solutions would involve errors in the analysis of kinetic parameters corresponding to this kind of biosensors. Provided the mediator species are in high concentration or diffuse much faster than the substrate, the response time of a biosensor of this kind would be determined by the diffusion of the substrate though the external membrane. In this sense, those systems with immobilized mediators, in which diffusion of electrons or holes is assumed for the charge transport process, could be also described by this model. Thus, the thickness and the permeability to the analyte of the external membrane are critical parameters for improving the response time of a sandwich-type biosensor. The simulated curves are compared with experimental profiles corresponding to a lactate amperometric biosensor obtaining consistent results. In a future publication a non-linear fitting algorithm will be combined to the model for the extraction of kinetic and/or geometric parameters.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20600
Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian; Mathematical modeling and experimental results of a sandwich-type amperometric biosensor; Elsevier Science; Sensors and Actuators B: Chemical; 162; 1; 12-2011; 284-291
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20600
identifier_str_mv Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian; Mathematical modeling and experimental results of a sandwich-type amperometric biosensor; Elsevier Science; Sensors and Actuators B: Chemical; 162; 1; 12-2011; 284-291
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2011.12.079
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400511011695
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.928904

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