An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples

Autores
Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Solis, Velia Matilde
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A biosensor based on the immobilization of lactate oxidase (LOx) on a glassy carbon electrode modified with laponite/chitosan hydrogels for the quantification of l-lactate in alcoholic beverages and dairy products is presented. Ferrocene-methanol (FcMe) is used as artificial mediator. The purpose of this work is to determine the best hydrogel composition from the analytical point of view. The characterization of the hydrogels was carried out by CV, amperometry and EIS. According to permeabilities and charge transfer resistances for ferrocyanide (used as molecular probe) as well as the enzymatic behavior of the enzyme for l-lactate, the best laponite/chitosan mass ratio found was 25/50. The distinct features of the bioelectrode are its long stability, its ability to reject or minimize most interferents including ascorbic acid, and its excellent analytical response, which allowed the reduction of the enzyme content below 0.5 U, for a sensitivity of (0.326 ± 0.003) A cm -2 M-1, with a time response lower than 5 s and a detection limit of (3.8 ± 0.2) × 10-6 M. Our l-lactate biosensor was validated by comparison with a standard spectroscopic method.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Solis, Velia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
BEER
DAIRY PRODUCTS
FERROCENE-METHANOL
LACTATE BIOSENSOR
LAPONITE/CHITOSAN HYDROGEL
WINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/188615
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/188615
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samplesPaz Zanini, Veronica IreneLopez, Beatriz AliciaSolis, Velia MatildeBEERDAIRY PRODUCTSFERROCENE-METHANOLLACTATE BIOSENSORLAPONITE/CHITOSAN HYDROGELWINEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A biosensor based on the immobilization of lactate oxidase (LOx) on a glassy carbon electrode modified with laponite/chitosan hydrogels for the quantification of l-lactate in alcoholic beverages and dairy products is presented. Ferrocene-methanol (FcMe) is used as artificial mediator. The purpose of this work is to determine the best hydrogel composition from the analytical point of view. The characterization of the hydrogels was carried out by CV, amperometry and EIS. According to permeabilities and charge transfer resistances for ferrocyanide (used as molecular probe) as well as the enzymatic behavior of the enzyme for l-lactate, the best laponite/chitosan mass ratio found was 25/50. The distinct features of the bioelectrode are its long stability, its ability to reject or minimize most interferents including ascorbic acid, and its excellent analytical response, which allowed the reduction of the enzyme content below 0.5 U, for a sensitivity of (0.326 ± 0.003) A cm -2 M-1, with a time response lower than 5 s and a detection limit of (3.8 ± 0.2) × 10-6 M. Our l-lactate biosensor was validated by comparison with a standard spectroscopic method.Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Solis, Velia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science SA2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/188615Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Solis, Velia Matilde; An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples; Elsevier Science SA; Sensors and Actuators B: Chemical; 155; 1; 7-2011; 75-800925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400510008956info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.11.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:29:19Zoai:ri.conicet.gov.ar:11336/188615instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:29:20.303CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
title An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
spellingShingle An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
Paz Zanini, Veronica Irene
BEER
DAIRY PRODUCTS
FERROCENE-METHANOL
LACTATE BIOSENSOR
LAPONITE/CHITOSAN HYDROGEL
WINE
title_short An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
title_full An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
title_fullStr An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
title_full_unstemmed An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
title_sort An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples
dc.creator.none.fl_str_mv Paz Zanini, Veronica Irene
Lopez, Beatriz Alicia
Solis, Velia Matilde
author Paz Zanini, Veronica Irene
author_facet Paz Zanini, Veronica Irene
Lopez, Beatriz Alicia
Solis, Velia Matilde
author_role author
author2 Lopez, Beatriz Alicia
Solis, Velia Matilde
author2_role author
author
dc.subject.none.fl_str_mv BEER
DAIRY PRODUCTS
FERROCENE-METHANOL
LACTATE BIOSENSOR
LAPONITE/CHITOSAN HYDROGEL
WINE
topic BEER
DAIRY PRODUCTS
FERROCENE-METHANOL
LACTATE BIOSENSOR
LAPONITE/CHITOSAN HYDROGEL
WINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A biosensor based on the immobilization of lactate oxidase (LOx) on a glassy carbon electrode modified with laponite/chitosan hydrogels for the quantification of l-lactate in alcoholic beverages and dairy products is presented. Ferrocene-methanol (FcMe) is used as artificial mediator. The purpose of this work is to determine the best hydrogel composition from the analytical point of view. The characterization of the hydrogels was carried out by CV, amperometry and EIS. According to permeabilities and charge transfer resistances for ferrocyanide (used as molecular probe) as well as the enzymatic behavior of the enzyme for l-lactate, the best laponite/chitosan mass ratio found was 25/50. The distinct features of the bioelectrode are its long stability, its ability to reject or minimize most interferents including ascorbic acid, and its excellent analytical response, which allowed the reduction of the enzyme content below 0.5 U, for a sensitivity of (0.326 ± 0.003) A cm -2 M-1, with a time response lower than 5 s and a detection limit of (3.8 ± 0.2) × 10-6 M. Our l-lactate biosensor was validated by comparison with a standard spectroscopic method.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Solis, Velia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description A biosensor based on the immobilization of lactate oxidase (LOx) on a glassy carbon electrode modified with laponite/chitosan hydrogels for the quantification of l-lactate in alcoholic beverages and dairy products is presented. Ferrocene-methanol (FcMe) is used as artificial mediator. The purpose of this work is to determine the best hydrogel composition from the analytical point of view. The characterization of the hydrogels was carried out by CV, amperometry and EIS. According to permeabilities and charge transfer resistances for ferrocyanide (used as molecular probe) as well as the enzymatic behavior of the enzyme for l-lactate, the best laponite/chitosan mass ratio found was 25/50. The distinct features of the bioelectrode are its long stability, its ability to reject or minimize most interferents including ascorbic acid, and its excellent analytical response, which allowed the reduction of the enzyme content below 0.5 U, for a sensitivity of (0.326 ± 0.003) A cm -2 M-1, with a time response lower than 5 s and a detection limit of (3.8 ± 0.2) × 10-6 M. Our l-lactate biosensor was validated by comparison with a standard spectroscopic method.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/188615
Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Solis, Velia Matilde; An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples; Elsevier Science SA; Sensors and Actuators B: Chemical; 155; 1; 7-2011; 75-80
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/188615
identifier_str_mv Paz Zanini, Veronica Irene; Lopez, Beatriz Alicia; Solis, Velia Matilde; An amperometric biosensor based on lactate oxidase immobilized in laponite-chitosan hydrogel on a glassy carbon electrode: Application to the analysis of l-lactate in food samples; Elsevier Science SA; Sensors and Actuators B: Chemical; 155; 1; 7-2011; 75-80
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400510008956
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.11.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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