Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase

Autores
Paz Zanini, Veronica Irene; Tulli, Fiorella Giovanna; Martino, Debora Marcela; Lopez, Beatriz Alicia; Borsarelli, Claudio Darío
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report the electroanalytical properties of an amperometric bioelectrode containing L-lactate oxidase (LOx) immobilized on glassy carbon electrode with a hydrogel film composed of laponite and different amounts of a novel bioinspired polycation obtained by copolimerization of 4-vinylbenzyl thymine (VBT) and 4-vinylbenzyl triethylammonium chloride (VBA) in a molar ratio 1:4, respectively. The electrochemical behavior of the redox couple probe [Fe(CN)6]3-/4-of these VBT-VBA bioelectrodes was compared with that observed for a bioelectrode containing the classical polycation polydiallyldimethylammonium chloride (PDDA). The best response was obtained for a biolelectrode containing a VBT-VBA/laponite mass ratio double than the cationic exchange capacity of the clay, demonstrating that under this condition the polycation induces an optimal microenvironment in the interlamellar space of the clay, both for the position and the functionality of LOx. The VBT-VBA bioelectrode displayed a very high sensitivity (7.2 ± 0.2) × 102 A.mM-1.cm-2, a short time response (<5 s), a wide linear response range (e.g. 0.01-1.0 mM of L-lactate) and an excellent stability over a storage period of 60 days, when sensing L-lactate. The analytical response of the bioelectrode was tested in real food samples, e.g. milk, white wine, and beer, as well as during milk fermentation at 37 °C. No effect of molecular interferences in the food matrices was detected, and the quantification of L-lactate was in complete agreement with standard assays reported values. Current results indicate that polycations containing the multifunctional green monomer VBT have high potential for their use in hydrogel film formation producing more responsive and stable electrochemical biosensors.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Tulli, Fiorella Giovanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Martino, Debora Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Borsarelli, Claudio Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Materia
L-Lactate
Bioelectrodes
Thymine
Dairy Products
Wine
Beer
Green Chemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7164
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7164
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidasePaz Zanini, Veronica IreneTulli, Fiorella GiovannaMartino, Debora MarcelaLopez, Beatriz AliciaBorsarelli, Claudio DaríoL-LactateBioelectrodesThymineDairy ProductsWineBeerGreen Chemistryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report the electroanalytical properties of an amperometric bioelectrode containing L-lactate oxidase (LOx) immobilized on glassy carbon electrode with a hydrogel film composed of laponite and different amounts of a novel bioinspired polycation obtained by copolimerization of 4-vinylbenzyl thymine (VBT) and 4-vinylbenzyl triethylammonium chloride (VBA) in a molar ratio 1:4, respectively. The electrochemical behavior of the redox couple probe [Fe(CN)6]3-/4-of these VBT-VBA bioelectrodes was compared with that observed for a bioelectrode containing the classical polycation polydiallyldimethylammonium chloride (PDDA). The best response was obtained for a biolelectrode containing a VBT-VBA/laponite mass ratio double than the cationic exchange capacity of the clay, demonstrating that under this condition the polycation induces an optimal microenvironment in the interlamellar space of the clay, both for the position and the functionality of LOx. The VBT-VBA bioelectrode displayed a very high sensitivity (7.2 ± 0.2) × 102 A.mM-1.cm-2, a short time response (<5 s), a wide linear response range (e.g. 0.01-1.0 mM of L-lactate) and an excellent stability over a storage period of 60 days, when sensing L-lactate. The analytical response of the bioelectrode was tested in real food samples, e.g. milk, white wine, and beer, as well as during milk fermentation at 37 °C. No effect of molecular interferences in the food matrices was detected, and the quantification of L-lactate was in complete agreement with standard assays reported values. Current results indicate that polycations containing the multifunctional green monomer VBT have high potential for their use in hydrogel film formation producing more responsive and stable electrochemical biosensors.Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Tulli, Fiorella Giovanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Martino, Debora Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); ArgentinaFil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Borsarelli, Claudio Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7164Paz Zanini, Veronica Irene; Tulli, Fiorella Giovanna; Martino, Debora Marcela; Lopez, Beatriz Alicia; Borsarelli, Claudio Darío; Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase; Elsevier; Sensors And Actuators B: Chemical; 181; 1-2013; 251-2580925-4005enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400513000786info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2013.01.061info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:23Zoai:ri.conicet.gov.ar:11336/7164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:23.548CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
title Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
spellingShingle Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
Paz Zanini, Veronica Irene
L-Lactate
Bioelectrodes
Thymine
Dairy Products
Wine
Beer
Green Chemistry
title_short Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
title_full Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
title_fullStr Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
title_full_unstemmed Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
title_sort Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase
dc.creator.none.fl_str_mv Paz Zanini, Veronica Irene
Tulli, Fiorella Giovanna
Martino, Debora Marcela
Lopez, Beatriz Alicia
Borsarelli, Claudio Darío
author Paz Zanini, Veronica Irene
author_facet Paz Zanini, Veronica Irene
Tulli, Fiorella Giovanna
Martino, Debora Marcela
Lopez, Beatriz Alicia
Borsarelli, Claudio Darío
author_role author
author2 Tulli, Fiorella Giovanna
Martino, Debora Marcela
Lopez, Beatriz Alicia
Borsarelli, Claudio Darío
author2_role author
author
author
author
dc.subject.none.fl_str_mv L-Lactate
Bioelectrodes
Thymine
Dairy Products
Wine
Beer
Green Chemistry
topic L-Lactate
Bioelectrodes
Thymine
Dairy Products
Wine
Beer
Green Chemistry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report the electroanalytical properties of an amperometric bioelectrode containing L-lactate oxidase (LOx) immobilized on glassy carbon electrode with a hydrogel film composed of laponite and different amounts of a novel bioinspired polycation obtained by copolimerization of 4-vinylbenzyl thymine (VBT) and 4-vinylbenzyl triethylammonium chloride (VBA) in a molar ratio 1:4, respectively. The electrochemical behavior of the redox couple probe [Fe(CN)6]3-/4-of these VBT-VBA bioelectrodes was compared with that observed for a bioelectrode containing the classical polycation polydiallyldimethylammonium chloride (PDDA). The best response was obtained for a biolelectrode containing a VBT-VBA/laponite mass ratio double than the cationic exchange capacity of the clay, demonstrating that under this condition the polycation induces an optimal microenvironment in the interlamellar space of the clay, both for the position and the functionality of LOx. The VBT-VBA bioelectrode displayed a very high sensitivity (7.2 ± 0.2) × 102 A.mM-1.cm-2, a short time response (<5 s), a wide linear response range (e.g. 0.01-1.0 mM of L-lactate) and an excellent stability over a storage period of 60 days, when sensing L-lactate. The analytical response of the bioelectrode was tested in real food samples, e.g. milk, white wine, and beer, as well as during milk fermentation at 37 °C. No effect of molecular interferences in the food matrices was detected, and the quantification of L-lactate was in complete agreement with standard assays reported values. Current results indicate that polycations containing the multifunctional green monomer VBT have high potential for their use in hydrogel film formation producing more responsive and stable electrochemical biosensors.
Fil: Paz Zanini, Veronica Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Tulli, Fiorella Giovanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Martino, Debora Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Lopez, Beatriz Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Borsarelli, Claudio Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
description We report the electroanalytical properties of an amperometric bioelectrode containing L-lactate oxidase (LOx) immobilized on glassy carbon electrode with a hydrogel film composed of laponite and different amounts of a novel bioinspired polycation obtained by copolimerization of 4-vinylbenzyl thymine (VBT) and 4-vinylbenzyl triethylammonium chloride (VBA) in a molar ratio 1:4, respectively. The electrochemical behavior of the redox couple probe [Fe(CN)6]3-/4-of these VBT-VBA bioelectrodes was compared with that observed for a bioelectrode containing the classical polycation polydiallyldimethylammonium chloride (PDDA). The best response was obtained for a biolelectrode containing a VBT-VBA/laponite mass ratio double than the cationic exchange capacity of the clay, demonstrating that under this condition the polycation induces an optimal microenvironment in the interlamellar space of the clay, both for the position and the functionality of LOx. The VBT-VBA bioelectrode displayed a very high sensitivity (7.2 ± 0.2) × 102 A.mM-1.cm-2, a short time response (<5 s), a wide linear response range (e.g. 0.01-1.0 mM of L-lactate) and an excellent stability over a storage period of 60 days, when sensing L-lactate. The analytical response of the bioelectrode was tested in real food samples, e.g. milk, white wine, and beer, as well as during milk fermentation at 37 °C. No effect of molecular interferences in the food matrices was detected, and the quantification of L-lactate was in complete agreement with standard assays reported values. Current results indicate that polycations containing the multifunctional green monomer VBT have high potential for their use in hydrogel film formation producing more responsive and stable electrochemical biosensors.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7164
Paz Zanini, Veronica Irene; Tulli, Fiorella Giovanna; Martino, Debora Marcela; Lopez, Beatriz Alicia; Borsarelli, Claudio Darío; Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase; Elsevier; Sensors And Actuators B: Chemical; 181; 1-2013; 251-258
0925-4005
url http://hdl.handle.net/11336/7164
identifier_str_mv Paz Zanini, Veronica Irene; Tulli, Fiorella Giovanna; Martino, Debora Marcela; Lopez, Beatriz Alicia; Borsarelli, Claudio Darío; Improvement of the amperometric response to l-lactate by using a cationic bioinspired thymine polycation in a bioelectrode with immobilized lactate oxidase; Elsevier; Sensors And Actuators B: Chemical; 181; 1-2013; 251-258
0925-4005
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400513000786
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2013.01.061
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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