Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response

Autores
Albarracín, Leonardo Miguel; Garcia Castillo, Valeria; Iida, Hikaru; Sato, Nana; Kanmani, Paulraj; Llabaca, Alejandra; Aso, Hisashi; García, Apolinaria; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lactobacillus rhamnosus CRL1505 and L. plantarum CRL1506 are immunomodulatory probiotic strains (immunobiotics) with the ability to improve the intestinal antiviral response and the protection against viral infections as demonstrated in animal models and clinical trials. In order to advance in the understanding of the mechanisms involved in the antiviral capacities of both immunobiotic strains, we have previously performed comparative transcriptomic studies in porcine intestinal epithelial (PIE cells). These cells were stimulated with L. rhamnosus CRL1505 or L. plantarum CRL1506 and then challenged with the TLR3 agonist poly(I:C). The immunotranscriptomic response of PIE cells was evaluated 12 h after TLR3 activation by poly(I:C) stimulation. Our results showed that both immunobiotic strains significantly improved the expression of IFN-α and IFN-β as well as the antiviral factors RNASE4, RNASEL, OAS1, OASL, MX1, and MX2 when compared to controls. In addition, both lactobacilli strains increased the expression of cytokines (IL-1β, IL-6), chemokines (CCL4, CCL20, CCL28, AMCF-II, CXCL10), and enzymes involved in prostaglandin biosynthesis (PTGES, PTGER4). For the majority of these genes, their expression in CRL1505-treated PIE cells was significantly higher than in cells treated with the CRL1506 strain. Furthermore, only L. rhamnosus CRL1505 differentially regulated the expression of CXCL2, CXCL5, and CXCL11. Then, our transcriptomic analysis successfully allowed us to identify a group of genes that can be used as prospective biomarkers for the screening of new antiviral immunobiotics in PIE cells (Albarracin et al, 2017, Front Immunol 8:57). The aim of this work was to evaluate whether the immunotranscriptomic changes induced by L. rhamnosus CRL1505 and L. plantarum CRL1506 in PIE cells are unique and not sheared by non-immunomodulatory strains of the same species.
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad de Concepción; Chile. Tohoku University; Japón
Fil: Iida, Hikaru. Tohoku University; Japón
Fil: Sato, Nana. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Llabaca, Alejandra. Universidad de Concepción; Chile
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: García, Apolinaria. Universidad de Concepción; Chile
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
XII Congress of the Latin American Association of Immunology; XXIII Congress of the Mexican Society of Immunology
Cancún
México
Latin American Association of Immunology
Mexican Society of Immunology
Materia
TLR3
BIOMARKERS
ANTIVIRAL RESPONSE
IMMUNOBIOTICS
PIE CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/246017
Acceder
id CONICETDig_8a5f4d34f51669e2c99610c5caadff08
oai_identifier_str oai:ri.conicet.gov.ar:11336/246017
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune responseAlbarracín, Leonardo MiguelGarcia Castillo, ValeriaIida, HikaruSato, NanaKanmani, PaulrajLlabaca, AlejandraAso, HisashiGarcía, ApolinariaKitazawa, HarukiVillena, Julio CesarTLR3BIOMARKERSANTIVIRAL RESPONSEIMMUNOBIOTICSPIE CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Lactobacillus rhamnosus CRL1505 and L. plantarum CRL1506 are immunomodulatory probiotic strains (immunobiotics) with the ability to improve the intestinal antiviral response and the protection against viral infections as demonstrated in animal models and clinical trials. In order to advance in the understanding of the mechanisms involved in the antiviral capacities of both immunobiotic strains, we have previously performed comparative transcriptomic studies in porcine intestinal epithelial (PIE cells). These cells were stimulated with L. rhamnosus CRL1505 or L. plantarum CRL1506 and then challenged with the TLR3 agonist poly(I:C). The immunotranscriptomic response of PIE cells was evaluated 12 h after TLR3 activation by poly(I:C) stimulation. Our results showed that both immunobiotic strains significantly improved the expression of IFN-α and IFN-β as well as the antiviral factors RNASE4, RNASEL, OAS1, OASL, MX1, and MX2 when compared to controls. In addition, both lactobacilli strains increased the expression of cytokines (IL-1β, IL-6), chemokines (CCL4, CCL20, CCL28, AMCF-II, CXCL10), and enzymes involved in prostaglandin biosynthesis (PTGES, PTGER4). For the majority of these genes, their expression in CRL1505-treated PIE cells was significantly higher than in cells treated with the CRL1506 strain. Furthermore, only L. rhamnosus CRL1505 differentially regulated the expression of CXCL2, CXCL5, and CXCL11. Then, our transcriptomic analysis successfully allowed us to identify a group of genes that can be used as prospective biomarkers for the screening of new antiviral immunobiotics in PIE cells (Albarracin et al, 2017, Front Immunol 8:57). The aim of this work was to evaluate whether the immunotranscriptomic changes induced by L. rhamnosus CRL1505 and L. plantarum CRL1506 in PIE cells are unique and not sheared by non-immunomodulatory strains of the same species.Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad de Concepción; Chile. Tohoku University; JapónFil: Iida, Hikaru. Tohoku University; JapónFil: Sato, Nana. Tohoku University; JapónFil: Kanmani, Paulraj. Tohoku University; JapónFil: Llabaca, Alejandra. Universidad de Concepción; ChileFil: Aso, Hisashi. Tohoku University; JapónFil: García, Apolinaria. Universidad de Concepción; ChileFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónXII Congress of the Latin American Association of Immunology; XXIII Congress of the Mexican Society of ImmunologyCancúnMéxicoLatin American Association of ImmunologyMexican Society of ImmunologyFrontiers Media2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/246017Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response; XII Congress of the Latin American Association of Immunology; XXIII Congress of the Mexican Society of Immunology; Cancún; México; 2018; 147-149978-2-88945-511-9CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/books/Immuno_Mexico_2018_XII_Congress_of_the_Latin_American_Association_of_Immunology_and_XXIII_Congress_/1637Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:51Zoai:ri.conicet.gov.ar:11336/246017instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:52.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
title Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
spellingShingle Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
Albarracín, Leonardo Miguel
TLR3
BIOMARKERS
ANTIVIRAL RESPONSE
IMMUNOBIOTICS
PIE CELLS
title_short Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
title_full Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
title_fullStr Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
title_full_unstemmed Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
title_sort Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response
dc.creator.none.fl_str_mv Albarracín, Leonardo Miguel
Garcia Castillo, Valeria
Iida, Hikaru
Sato, Nana
Kanmani, Paulraj
Llabaca, Alejandra
Aso, Hisashi
García, Apolinaria
Kitazawa, Haruki
Villena, Julio Cesar
author Albarracín, Leonardo Miguel
author_facet Albarracín, Leonardo Miguel
Garcia Castillo, Valeria
Iida, Hikaru
Sato, Nana
Kanmani, Paulraj
Llabaca, Alejandra
Aso, Hisashi
García, Apolinaria
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Garcia Castillo, Valeria
Iida, Hikaru
Sato, Nana
Kanmani, Paulraj
Llabaca, Alejandra
Aso, Hisashi
García, Apolinaria
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TLR3
BIOMARKERS
ANTIVIRAL RESPONSE
IMMUNOBIOTICS
PIE CELLS
topic TLR3
BIOMARKERS
ANTIVIRAL RESPONSE
IMMUNOBIOTICS
PIE CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lactobacillus rhamnosus CRL1505 and L. plantarum CRL1506 are immunomodulatory probiotic strains (immunobiotics) with the ability to improve the intestinal antiviral response and the protection against viral infections as demonstrated in animal models and clinical trials. In order to advance in the understanding of the mechanisms involved in the antiviral capacities of both immunobiotic strains, we have previously performed comparative transcriptomic studies in porcine intestinal epithelial (PIE cells). These cells were stimulated with L. rhamnosus CRL1505 or L. plantarum CRL1506 and then challenged with the TLR3 agonist poly(I:C). The immunotranscriptomic response of PIE cells was evaluated 12 h after TLR3 activation by poly(I:C) stimulation. Our results showed that both immunobiotic strains significantly improved the expression of IFN-α and IFN-β as well as the antiviral factors RNASE4, RNASEL, OAS1, OASL, MX1, and MX2 when compared to controls. In addition, both lactobacilli strains increased the expression of cytokines (IL-1β, IL-6), chemokines (CCL4, CCL20, CCL28, AMCF-II, CXCL10), and enzymes involved in prostaglandin biosynthesis (PTGES, PTGER4). For the majority of these genes, their expression in CRL1505-treated PIE cells was significantly higher than in cells treated with the CRL1506 strain. Furthermore, only L. rhamnosus CRL1505 differentially regulated the expression of CXCL2, CXCL5, and CXCL11. Then, our transcriptomic analysis successfully allowed us to identify a group of genes that can be used as prospective biomarkers for the screening of new antiviral immunobiotics in PIE cells (Albarracin et al, 2017, Front Immunol 8:57). The aim of this work was to evaluate whether the immunotranscriptomic changes induced by L. rhamnosus CRL1505 and L. plantarum CRL1506 in PIE cells are unique and not sheared by non-immunomodulatory strains of the same species.
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad de Concepción; Chile. Tohoku University; Japón
Fil: Iida, Hikaru. Tohoku University; Japón
Fil: Sato, Nana. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Llabaca, Alejandra. Universidad de Concepción; Chile
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: García, Apolinaria. Universidad de Concepción; Chile
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
XII Congress of the Latin American Association of Immunology; XXIII Congress of the Mexican Society of Immunology
Cancún
México
Latin American Association of Immunology
Mexican Society of Immunology
description Lactobacillus rhamnosus CRL1505 and L. plantarum CRL1506 are immunomodulatory probiotic strains (immunobiotics) with the ability to improve the intestinal antiviral response and the protection against viral infections as demonstrated in animal models and clinical trials. In order to advance in the understanding of the mechanisms involved in the antiviral capacities of both immunobiotic strains, we have previously performed comparative transcriptomic studies in porcine intestinal epithelial (PIE cells). These cells were stimulated with L. rhamnosus CRL1505 or L. plantarum CRL1506 and then challenged with the TLR3 agonist poly(I:C). The immunotranscriptomic response of PIE cells was evaluated 12 h after TLR3 activation by poly(I:C) stimulation. Our results showed that both immunobiotic strains significantly improved the expression of IFN-α and IFN-β as well as the antiviral factors RNASE4, RNASEL, OAS1, OASL, MX1, and MX2 when compared to controls. In addition, both lactobacilli strains increased the expression of cytokines (IL-1β, IL-6), chemokines (CCL4, CCL20, CCL28, AMCF-II, CXCL10), and enzymes involved in prostaglandin biosynthesis (PTGES, PTGER4). For the majority of these genes, their expression in CRL1505-treated PIE cells was significantly higher than in cells treated with the CRL1506 strain. Furthermore, only L. rhamnosus CRL1505 differentially regulated the expression of CXCL2, CXCL5, and CXCL11. Then, our transcriptomic analysis successfully allowed us to identify a group of genes that can be used as prospective biomarkers for the screening of new antiviral immunobiotics in PIE cells (Albarracin et al, 2017, Front Immunol 8:57). The aim of this work was to evaluate whether the immunotranscriptomic changes induced by L. rhamnosus CRL1505 and L. plantarum CRL1506 in PIE cells are unique and not sheared by non-immunomodulatory strains of the same species.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/246017
Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response; XII Congress of the Latin American Association of Immunology; XXIII Congress of the Mexican Society of Immunology; Cancún; México; 2018; 147-149
978-2-88945-511-9
CONICET Digital
CONICET
url http://hdl.handle.net/11336/246017
identifier_str_mv Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response; XII Congress of the Latin American Association of Immunology; XXIII Congress of the Mexican Society of Immunology; Cancún; México; 2018; 147-149
978-2-88945-511-9
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/books/Immuno_Mexico_2018_XII_Congress_of_the_Latin_American_Association_of_Immunology_and_XXIII_Congress_/1637
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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