Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells

Autores
Iida, Hikaru; Tohno, Masanori; Islam, Md. Aminul; Sato, Nana; Kobayashi, Hisakazu; Albarracín, Leonardo Miguel; Humayun Kober, AKM; Ikeda-Ohtsubo, Wakako; Suda, Yoshihito; Aso, Hisashi; Nochi, Tomonori; Miyazaki, Ayako; Uenishi, Hirohide; Iwabuchi, Noriyuki; Xiao, Jin-zhong; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer?s patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.
Fil: Iida, Hikaru. Tohoku University; Japón
Fil: Tohno, Masanori. National Agriculture and Food Research Organization. Central Region Agricultural Research Centre; Japón
Fil: Islam, Md. Aminul. Tohoku University; Japón. Agricultural University. Faculty of Veterinary Science. Department of Medicine; Bangladesh
Fil: Sato, Nana. Tohoku University; Japón
Fil: Kobayashi, Hisakazu. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Humayun Kober, AKM. Tohoku University; Japón
Fil: Ikeda-Ohtsubo, Wakako. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environment; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Miyazaki, Ayako. National Institute of Animal Health. Viral Diseases and Epidemiology Research Division; Japón
Fil: Uenishi, Hirohide. National Agriculture And Food Research Organization; Japón
Fil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co., Ltd.; Japón
Fil: Xiao, Jin-zhong. Morinaga Milk Industry Co., Ltd.; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
PARAIMMUNOBIOTICS
PEPTIDOGLYCAN RECOGNITION PROTEIN
BIFIDOBACTERIA
INNATE IMMUNITY
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE ANTIGEN-PRESENTING CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97175
Acceder
id CONICETDig_c70a985212250398428a6a514a9c2db1
oai_identifier_str oai:ri.conicet.gov.ar:11336/97175
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ellsIida, HikaruTohno, MasanoriIslam, Md. AminulSato, NanaKobayashi, HisakazuAlbarracín, Leonardo MiguelHumayun Kober, AKMIkeda-Ohtsubo, WakakoSuda, YoshihitoAso, HisashiNochi, TomonoriMiyazaki, AyakoUenishi, HirohideIwabuchi, NoriyukiXiao, Jin-zhongVillena, Julio CesarKitazawa, HarukiPARAIMMUNOBIOTICSPEPTIDOGLYCAN RECOGNITION PROTEINBIFIDOBACTERIAINNATE IMMUNITYPORCINE INTESTINAL EPITHELIOCYTESPORCINE ANTIGEN-PRESENTING CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer?s patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.Fil: Iida, Hikaru. Tohoku University; JapónFil: Tohno, Masanori. National Agriculture and Food Research Organization. Central Region Agricultural Research Centre; JapónFil: Islam, Md. Aminul. Tohoku University; Japón. Agricultural University. Faculty of Veterinary Science. Department of Medicine; BangladeshFil: Sato, Nana. Tohoku University; JapónFil: Kobayashi, Hisakazu. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Humayun Kober, AKM. Tohoku University; JapónFil: Ikeda-Ohtsubo, Wakako. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environment; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Nochi, Tomonori. Tohoku University; JapónFil: Miyazaki, Ayako. National Institute of Animal Health. Viral Diseases and Epidemiology Research Division; JapónFil: Uenishi, Hirohide. National Agriculture And Food Research Organization; JapónFil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co., Ltd.; JapónFil: Xiao, Jin-zhong. Morinaga Milk Industry Co., Ltd.; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónMDPI2019-08-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97175Iida, Hikaru; Tohno, Masanori; Islam, Md. Aminul; Sato, Nana; Kobayashi, Hisakazu; et al.; Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells; MDPI; Cells; 8; 8; 14-8-2019; 1-22;891-8912073-4409CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4409/8/8/891info:eu-repo/semantics/altIdentifier/doi/10.3390/cells8080891info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:08Zoai:ri.conicet.gov.ar:11336/97175instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:08.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
title Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
spellingShingle Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
Iida, Hikaru
PARAIMMUNOBIOTICS
PEPTIDOGLYCAN RECOGNITION PROTEIN
BIFIDOBACTERIA
INNATE IMMUNITY
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE ANTIGEN-PRESENTING CELLS
title_short Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
title_full Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
title_fullStr Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
title_full_unstemmed Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
title_sort Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells
dc.creator.none.fl_str_mv Iida, Hikaru
Tohno, Masanori
Islam, Md. Aminul
Sato, Nana
Kobayashi, Hisakazu
Albarracín, Leonardo Miguel
Humayun Kober, AKM
Ikeda-Ohtsubo, Wakako
Suda, Yoshihito
Aso, Hisashi
Nochi, Tomonori
Miyazaki, Ayako
Uenishi, Hirohide
Iwabuchi, Noriyuki
Xiao, Jin-zhong
Villena, Julio Cesar
Kitazawa, Haruki
author Iida, Hikaru
author_facet Iida, Hikaru
Tohno, Masanori
Islam, Md. Aminul
Sato, Nana
Kobayashi, Hisakazu
Albarracín, Leonardo Miguel
Humayun Kober, AKM
Ikeda-Ohtsubo, Wakako
Suda, Yoshihito
Aso, Hisashi
Nochi, Tomonori
Miyazaki, Ayako
Uenishi, Hirohide
Iwabuchi, Noriyuki
Xiao, Jin-zhong
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Tohno, Masanori
Islam, Md. Aminul
Sato, Nana
Kobayashi, Hisakazu
Albarracín, Leonardo Miguel
Humayun Kober, AKM
Ikeda-Ohtsubo, Wakako
Suda, Yoshihito
Aso, Hisashi
Nochi, Tomonori
Miyazaki, Ayako
Uenishi, Hirohide
Iwabuchi, Noriyuki
Xiao, Jin-zhong
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PARAIMMUNOBIOTICS
PEPTIDOGLYCAN RECOGNITION PROTEIN
BIFIDOBACTERIA
INNATE IMMUNITY
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE ANTIGEN-PRESENTING CELLS
topic PARAIMMUNOBIOTICS
PEPTIDOGLYCAN RECOGNITION PROTEIN
BIFIDOBACTERIA
INNATE IMMUNITY
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE ANTIGEN-PRESENTING CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer?s patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.
Fil: Iida, Hikaru. Tohoku University; Japón
Fil: Tohno, Masanori. National Agriculture and Food Research Organization. Central Region Agricultural Research Centre; Japón
Fil: Islam, Md. Aminul. Tohoku University; Japón. Agricultural University. Faculty of Veterinary Science. Department of Medicine; Bangladesh
Fil: Sato, Nana. Tohoku University; Japón
Fil: Kobayashi, Hisakazu. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Humayun Kober, AKM. Tohoku University; Japón
Fil: Ikeda-Ohtsubo, Wakako. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environment; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Miyazaki, Ayako. National Institute of Animal Health. Viral Diseases and Epidemiology Research Division; Japón
Fil: Uenishi, Hirohide. National Agriculture And Food Research Organization; Japón
Fil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co., Ltd.; Japón
Fil: Xiao, Jin-zhong. Morinaga Milk Industry Co., Ltd.; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Peptidoglycan recognition proteins (PGLYRPs) are a family of pattern recognition receptors (PRRs) that are able to induce innate immune responses through their binding to peptidoglycan (PGN), lipopolysaccharide, or lipoteichoic acid, or by interacting with other PRR-ligands. Recently, progress has been made in understanding the immunobiology of PGLYRPs in human and mice, however, their functions in livestock animals have been less explored. In this study, we characterized the expression patterns of PGLYRPs in porcine intestinal epithelial (PIE) cells and antigen-presenting cells (APCs) and their modulation by the interactions of host cells with PRR-ligands and non-viable immunomodulatory probiotics referred to as paraimmunobiotics. We demonstrated that PGLYRP-1, -2, -3, and -4 are expressed in PIE cells and APCs from Peyer?s patches, being PGLYPR-3 and -4 levels higher than PGLYRP-1 and -2. We also showed that PGLYRPs expression in APCs and PIE cells can be modulated by different PRR agonists. By using knockdown PIE cells for TLR2, TLR4, NOD1, and NOD2, or the four PGLYRPs, we demonstrated that PGLYRPs expressions would be required for activation and functioning of TLR2, TLR4, NOD1, and NOD2 in porcine epitheliocytes, but PGLYRPs activation would be independent of those PRR expressions. Importantly, we reported for the first time that PGLYRPs expression can be differentially modulated by paraimmunobiotic bifidobacteria in a strain-dependent manner. These results provide evidence for the use of paraimmunobiotic bifidobacteria as an alternative for the improvement of resistance to intestinal infections or as therapeutic tools for the reduction of the severity of inflammatory damage in diseases in which a role of PGLYRPs-microbe interaction has been demonstrated.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97175
Iida, Hikaru; Tohno, Masanori; Islam, Md. Aminul; Sato, Nana; Kobayashi, Hisakazu; et al.; Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells; MDPI; Cells; 8; 8; 14-8-2019; 1-22;891-891
2073-4409
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97175
identifier_str_mv Iida, Hikaru; Tohno, Masanori; Islam, Md. Aminul; Sato, Nana; Kobayashi, Hisakazu; et al.; Paraimmunobiotic bifidobacteria modulate the expression patterns of peptidoglycan recognition proteins in porcine intestinal epitheliocytes and antigen presenting ells; MDPI; Cells; 8; 8; 14-8-2019; 1-22;891-891
2073-4409
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4409/8/8/891
info:eu-repo/semantics/altIdentifier/doi/10.3390/cells8080891
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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