Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting

Autores
Sauka, Diego Herman; Basile, Juan Ignacio; Benintende, Graciela Beatriz
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacillus thuringiensis is classified into serovars on the basis of H-flagellar antigens. Several alternative typing methods have been described. Among them, a B. cereus group-specific repetitive extragenic palindromic (Rep)-PCR fingerprinting technique was shown to be discriminative and able to identify B. thuringiensis serovars. The aim of this study was to investigate the genomic diversity and relationship among B. thuringiensis strains collected from different Argentinean ecosystems. Thirty-seven B. thuringiensis reference strains and 131 Argentinean isolates were analyzed using a B. cereus group-specific Rep-PCR. Fourteen different patterns were identified among the Argentinean isolates. Eight could not be associated to any pattern obtained from a reference strain. The pattern identical to the serovar kurstaki HD-1 strain was the most frequently identified in 68 native isolates. The profiles allowed tracing a single dendrogram with two groups and eight main lineages. Some strains showed distinctive patterns despite belonging to the same serovar. An intraspecific diversity resulted from this analysis that was highlighted by this technique since strains from a given serovar showed distinct profiles. This study may help to establish a system of B. thuringiensis classification with a higher discrimination level than established by the H antigen serotyping. Copyright © 2012 S. Karger AG, Basel.
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Basile, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Materia
Argentinean Isolates
Bacillus Thuringiensis
Fingerprinting
Intra-Serovar Diversity
Rep-Pcr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53316
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53316
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprintingSauka, Diego HermanBasile, Juan IgnacioBenintende, Graciela BeatrizArgentinean IsolatesBacillus ThuringiensisFingerprintingIntra-Serovar DiversityRep-Pcrhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacillus thuringiensis is classified into serovars on the basis of H-flagellar antigens. Several alternative typing methods have been described. Among them, a B. cereus group-specific repetitive extragenic palindromic (Rep)-PCR fingerprinting technique was shown to be discriminative and able to identify B. thuringiensis serovars. The aim of this study was to investigate the genomic diversity and relationship among B. thuringiensis strains collected from different Argentinean ecosystems. Thirty-seven B. thuringiensis reference strains and 131 Argentinean isolates were analyzed using a B. cereus group-specific Rep-PCR. Fourteen different patterns were identified among the Argentinean isolates. Eight could not be associated to any pattern obtained from a reference strain. The pattern identical to the serovar kurstaki HD-1 strain was the most frequently identified in 68 native isolates. The profiles allowed tracing a single dendrogram with two groups and eight main lineages. Some strains showed distinctive patterns despite belonging to the same serovar. An intraspecific diversity resulted from this analysis that was highlighted by this technique since strains from a given serovar showed distinct profiles. This study may help to establish a system of B. thuringiensis classification with a higher discrimination level than established by the H antigen serotyping. Copyright © 2012 S. Karger AG, Basel.Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Basile, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaKarger2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53316Sauka, Diego Herman; Basile, Juan Ignacio; Benintende, Graciela Beatriz; Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting; Karger; Journal of Molecular Microbiology and Biotechnology; 21; 3-4; 1-2012; 184-1901464-1801CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000335532info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/335532info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:18:46Zoai:ri.conicet.gov.ar:11336/53316instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:18:46.783CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
title Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
spellingShingle Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
Sauka, Diego Herman
Argentinean Isolates
Bacillus Thuringiensis
Fingerprinting
Intra-Serovar Diversity
Rep-Pcr
title_short Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
title_full Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
title_fullStr Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
title_full_unstemmed Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
title_sort Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting
dc.creator.none.fl_str_mv Sauka, Diego Herman
Basile, Juan Ignacio
Benintende, Graciela Beatriz
author Sauka, Diego Herman
author_facet Sauka, Diego Herman
Basile, Juan Ignacio
Benintende, Graciela Beatriz
author_role author
author2 Basile, Juan Ignacio
Benintende, Graciela Beatriz
author2_role author
author
dc.subject.none.fl_str_mv Argentinean Isolates
Bacillus Thuringiensis
Fingerprinting
Intra-Serovar Diversity
Rep-Pcr
topic Argentinean Isolates
Bacillus Thuringiensis
Fingerprinting
Intra-Serovar Diversity
Rep-Pcr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacillus thuringiensis is classified into serovars on the basis of H-flagellar antigens. Several alternative typing methods have been described. Among them, a B. cereus group-specific repetitive extragenic palindromic (Rep)-PCR fingerprinting technique was shown to be discriminative and able to identify B. thuringiensis serovars. The aim of this study was to investigate the genomic diversity and relationship among B. thuringiensis strains collected from different Argentinean ecosystems. Thirty-seven B. thuringiensis reference strains and 131 Argentinean isolates were analyzed using a B. cereus group-specific Rep-PCR. Fourteen different patterns were identified among the Argentinean isolates. Eight could not be associated to any pattern obtained from a reference strain. The pattern identical to the serovar kurstaki HD-1 strain was the most frequently identified in 68 native isolates. The profiles allowed tracing a single dendrogram with two groups and eight main lineages. Some strains showed distinctive patterns despite belonging to the same serovar. An intraspecific diversity resulted from this analysis that was highlighted by this technique since strains from a given serovar showed distinct profiles. This study may help to establish a system of B. thuringiensis classification with a higher discrimination level than established by the H antigen serotyping. Copyright © 2012 S. Karger AG, Basel.
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Basile, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
description Bacillus thuringiensis is classified into serovars on the basis of H-flagellar antigens. Several alternative typing methods have been described. Among them, a B. cereus group-specific repetitive extragenic palindromic (Rep)-PCR fingerprinting technique was shown to be discriminative and able to identify B. thuringiensis serovars. The aim of this study was to investigate the genomic diversity and relationship among B. thuringiensis strains collected from different Argentinean ecosystems. Thirty-seven B. thuringiensis reference strains and 131 Argentinean isolates were analyzed using a B. cereus group-specific Rep-PCR. Fourteen different patterns were identified among the Argentinean isolates. Eight could not be associated to any pattern obtained from a reference strain. The pattern identical to the serovar kurstaki HD-1 strain was the most frequently identified in 68 native isolates. The profiles allowed tracing a single dendrogram with two groups and eight main lineages. Some strains showed distinctive patterns despite belonging to the same serovar. An intraspecific diversity resulted from this analysis that was highlighted by this technique since strains from a given serovar showed distinct profiles. This study may help to establish a system of B. thuringiensis classification with a higher discrimination level than established by the H antigen serotyping. Copyright © 2012 S. Karger AG, Basel.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53316
Sauka, Diego Herman; Basile, Juan Ignacio; Benintende, Graciela Beatriz; Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting; Karger; Journal of Molecular Microbiology and Biotechnology; 21; 3-4; 1-2012; 184-190
1464-1801
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53316
identifier_str_mv Sauka, Diego Herman; Basile, Juan Ignacio; Benintende, Graciela Beatriz; Evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR genomic fingerprinting; Karger; Journal of Molecular Microbiology and Biotechnology; 21; 3-4; 1-2012; 184-190
1464-1801
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000335532
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/335532
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.928904

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