Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus

Autores
Palazzolo, Martín Alejandro; Mascotti, María Laura; Lewkowicz, Elizabeth Sandra; Kurina Sanz, Marcela Beatriz
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aromatic carboxylic acids are readily obtained from lignin in biomass processing facilities. However, efficient technologies for lignin valorization are missing. In this work, a microbial screening was conducted to find versatile biocatalysts capable of transforming several benzoic acids structurally related to lignin, employing vanillic acid as model substrate. The wild-type Aspergillus flavus growing cells exhibited exquisite selectivity towards the oxidative decarboxylation product, 2-methoxybenzene-1,4-diol. Interestingly, when assaying a set of structurally-related substrates, the biocatalyst displayed the oxidative removal of the carboxyl moiety or its reduction to the primary alcohol whether electron withdrawing or donating groups were present in the aromatic ring, respectively. Additionally, A. flavus proved to be highly tolerant to vanillic acid increasing concentrations (up to 8 g/L), demonstrating its potential application in chemical synthesis. A. flavus growing cells were found to be efficient biotechnological tools to perform self-sufficient, structure-dependent redox reactions. All the biotransformations described herein are reported for the first time.
Fil: Palazzolo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones En Tecnología Química; Argentina
Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Lewkowicz, Elizabeth Sandra. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia; Argentina
Fil: Kurina Sanz, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones En Tecnología Química; Argentina
Materia
Benzoic
Redox Biotransformation
Aspergillus Flavus
Acids Carboxyl Reduction
Oxidative Decarboxylation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5843
Acceder
id CONICETDig_da7379f269c2017e7be638b4cdccb39b
oai_identifier_str oai:ri.conicet.gov.ar:11336/5843
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavusPalazzolo, Martín AlejandroMascotti, María LauraLewkowicz, Elizabeth SandraKurina Sanz, Marcela BeatrizBenzoicRedox BiotransformationAspergillus FlavusAcids Carboxyl ReductionOxidative Decarboxylationhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Aromatic carboxylic acids are readily obtained from lignin in biomass processing facilities. However, efficient technologies for lignin valorization are missing. In this work, a microbial screening was conducted to find versatile biocatalysts capable of transforming several benzoic acids structurally related to lignin, employing vanillic acid as model substrate. The wild-type Aspergillus flavus growing cells exhibited exquisite selectivity towards the oxidative decarboxylation product, 2-methoxybenzene-1,4-diol. Interestingly, when assaying a set of structurally-related substrates, the biocatalyst displayed the oxidative removal of the carboxyl moiety or its reduction to the primary alcohol whether electron withdrawing or donating groups were present in the aromatic ring, respectively. Additionally, A. flavus proved to be highly tolerant to vanillic acid increasing concentrations (up to 8 g/L), demonstrating its potential application in chemical synthesis. A. flavus growing cells were found to be efficient biotechnological tools to perform self-sufficient, structure-dependent redox reactions. All the biotransformations described herein are reported for the first time.Fil: Palazzolo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones En Tecnología Química; ArgentinaFil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Lewkowicz, Elizabeth Sandra. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia; ArgentinaFil: Kurina Sanz, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones En Tecnología Química; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5843Palazzolo, Martín Alejandro; Mascotti, María Laura; Lewkowicz, Elizabeth Sandra; Kurina Sanz, Marcela Beatriz; Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus; Springer; Journal of Industrial Microbiology & Biotechnology; 42; 12; 10-2015; 1581-15891367-5435enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10295-015-1696-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s10295-015-1696-4info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:19Zoai:ri.conicet.gov.ar:11336/5843instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:19.948CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
title Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
spellingShingle Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
Palazzolo, Martín Alejandro
Benzoic
Redox Biotransformation
Aspergillus Flavus
Acids Carboxyl Reduction
Oxidative Decarboxylation
title_short Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
title_full Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
title_fullStr Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
title_full_unstemmed Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
title_sort Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
dc.creator.none.fl_str_mv Palazzolo, Martín Alejandro
Mascotti, María Laura
Lewkowicz, Elizabeth Sandra
Kurina Sanz, Marcela Beatriz
author Palazzolo, Martín Alejandro
author_facet Palazzolo, Martín Alejandro
Mascotti, María Laura
Lewkowicz, Elizabeth Sandra
Kurina Sanz, Marcela Beatriz
author_role author
author2 Mascotti, María Laura
Lewkowicz, Elizabeth Sandra
Kurina Sanz, Marcela Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv Benzoic
Redox Biotransformation
Aspergillus Flavus
Acids Carboxyl Reduction
Oxidative Decarboxylation
topic Benzoic
Redox Biotransformation
Aspergillus Flavus
Acids Carboxyl Reduction
Oxidative Decarboxylation
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Aromatic carboxylic acids are readily obtained from lignin in biomass processing facilities. However, efficient technologies for lignin valorization are missing. In this work, a microbial screening was conducted to find versatile biocatalysts capable of transforming several benzoic acids structurally related to lignin, employing vanillic acid as model substrate. The wild-type Aspergillus flavus growing cells exhibited exquisite selectivity towards the oxidative decarboxylation product, 2-methoxybenzene-1,4-diol. Interestingly, when assaying a set of structurally-related substrates, the biocatalyst displayed the oxidative removal of the carboxyl moiety or its reduction to the primary alcohol whether electron withdrawing or donating groups were present in the aromatic ring, respectively. Additionally, A. flavus proved to be highly tolerant to vanillic acid increasing concentrations (up to 8 g/L), demonstrating its potential application in chemical synthesis. A. flavus growing cells were found to be efficient biotechnological tools to perform self-sufficient, structure-dependent redox reactions. All the biotransformations described herein are reported for the first time.
Fil: Palazzolo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones En Tecnología Química; Argentina
Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Lewkowicz, Elizabeth Sandra. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia; Argentina
Fil: Kurina Sanz, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones En Tecnología Química; Argentina
description Aromatic carboxylic acids are readily obtained from lignin in biomass processing facilities. However, efficient technologies for lignin valorization are missing. In this work, a microbial screening was conducted to find versatile biocatalysts capable of transforming several benzoic acids structurally related to lignin, employing vanillic acid as model substrate. The wild-type Aspergillus flavus growing cells exhibited exquisite selectivity towards the oxidative decarboxylation product, 2-methoxybenzene-1,4-diol. Interestingly, when assaying a set of structurally-related substrates, the biocatalyst displayed the oxidative removal of the carboxyl moiety or its reduction to the primary alcohol whether electron withdrawing or donating groups were present in the aromatic ring, respectively. Additionally, A. flavus proved to be highly tolerant to vanillic acid increasing concentrations (up to 8 g/L), demonstrating its potential application in chemical synthesis. A. flavus growing cells were found to be efficient biotechnological tools to perform self-sufficient, structure-dependent redox reactions. All the biotransformations described herein are reported for the first time.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5843
Palazzolo, Martín Alejandro; Mascotti, María Laura; Lewkowicz, Elizabeth Sandra; Kurina Sanz, Marcela Beatriz; Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus; Springer; Journal of Industrial Microbiology & Biotechnology; 42; 12; 10-2015; 1581-1589
1367-5435
url http://hdl.handle.net/11336/5843
identifier_str_mv Palazzolo, Martín Alejandro; Mascotti, María Laura; Lewkowicz, Elizabeth Sandra; Kurina Sanz, Marcela Beatriz; Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus; Springer; Journal of Industrial Microbiology & Biotechnology; 42; 12; 10-2015; 1581-1589
1367-5435
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10295-015-1696-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10295-015-1696-4
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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