Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats

Autores
García, María del Carmen; Godoy, Yanina Cynthia; Celuch, Stella Maris
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Blood pressure responses to intrathecal (i.t.) injection of neurochemicals were examined in the fructose-fed rat, an experimental model of metabolic syndrome.Sprague-Dawley rats receiving either tap water or water containing 10% fructose during 8 weeks were anesthetized with sodium pentobarbital. The endocannabinoid anandamide (100 nmol; i.t.) decreased mean blood pressure in control rats (21.276.3 mmHg), but had no effect in fructose-fed animals. Similarly, calcitonin generelated peptide (CGRP; 0.125 nmol; i.t.) decreased mean blood pressure in control, but not in treated rats. The high fructose diet did not cause significant changes in the pressor effects of i.t. administered noradrenaline (100 nmol) and N-methyl-D-aspartate (30 nmol). The nitric oxide donor sodium nitroprusside (500 nmol, i.t.) induced a brief hypotension followed by a sustained increase in mean blood pressure in control rats; however, this drug only produced pressor effects in fructose-fed animals. The GABAA-receptor agonist muscimol (8.8 nmol, i.t.) and the GABAB-receptor agonist baclofen (100 nmol, i.t.) decreased mean blood pressure 30–35 mmHg, both in control and in fructose-fed rats. Fructose potentiated the pressor effect of i.v. injected noradrenaline, but did not modify the hypotensive responses to i.v. administered sodium nitroprusside and acetylcholine.These results could suggest that, in pentobarbital-anesthetized rats, fructose feeding could alter spinal mechanisms of regulation of preganglionic sympathetic nerve activity. It is proposed that the spinal cord could be involved in the sympathetic dysfunction associated with the metabolic syndrome.
Fil: García, María del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: Godoy, Yanina Cynthia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Celuch, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina
Materia
Metabolic Syndrome
Fructose
Spinal Cord
Blood Pressure
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12443
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed ratsGarcía, María del CarmenGodoy, Yanina CynthiaCeluch, Stella MarisMetabolic SyndromeFructoseSpinal CordBlood Pressurehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Blood pressure responses to intrathecal (i.t.) injection of neurochemicals were examined in the fructose-fed rat, an experimental model of metabolic syndrome.Sprague-Dawley rats receiving either tap water or water containing 10% fructose during 8 weeks were anesthetized with sodium pentobarbital. The endocannabinoid anandamide (100 nmol; i.t.) decreased mean blood pressure in control rats (21.276.3 mmHg), but had no effect in fructose-fed animals. Similarly, calcitonin generelated peptide (CGRP; 0.125 nmol; i.t.) decreased mean blood pressure in control, but not in treated rats. The high fructose diet did not cause significant changes in the pressor effects of i.t. administered noradrenaline (100 nmol) and N-methyl-D-aspartate (30 nmol). The nitric oxide donor sodium nitroprusside (500 nmol, i.t.) induced a brief hypotension followed by a sustained increase in mean blood pressure in control rats; however, this drug only produced pressor effects in fructose-fed animals. The GABAA-receptor agonist muscimol (8.8 nmol, i.t.) and the GABAB-receptor agonist baclofen (100 nmol, i.t.) decreased mean blood pressure 30–35 mmHg, both in control and in fructose-fed rats. Fructose potentiated the pressor effect of i.v. injected noradrenaline, but did not modify the hypotensive responses to i.v. administered sodium nitroprusside and acetylcholine.These results could suggest that, in pentobarbital-anesthetized rats, fructose feeding could alter spinal mechanisms of regulation of preganglionic sympathetic nerve activity. It is proposed that the spinal cord could be involved in the sympathetic dysfunction associated with the metabolic syndrome.Fil: García, María del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; ArgentinaFil: Godoy, Yanina Cynthia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Celuch, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); ArgentinaElsevier Science2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12443García, María del Carmen; Godoy, Yanina Cynthia; Celuch, Stella Maris; Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats; Elsevier Science; European Journal Of Pharmacology; 706; 1-3; 4-2013; 17-240014-2999enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2013.02.037info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014299913001465info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:34Zoai:ri.conicet.gov.ar:11336/12443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:34.438CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
title Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
spellingShingle Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
García, María del Carmen
Metabolic Syndrome
Fructose
Spinal Cord
Blood Pressure
title_short Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
title_full Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
title_fullStr Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
title_full_unstemmed Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
title_sort Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats
dc.creator.none.fl_str_mv García, María del Carmen
Godoy, Yanina Cynthia
Celuch, Stella Maris
author García, María del Carmen
author_facet García, María del Carmen
Godoy, Yanina Cynthia
Celuch, Stella Maris
author_role author
author2 Godoy, Yanina Cynthia
Celuch, Stella Maris
author2_role author
author
dc.subject.none.fl_str_mv Metabolic Syndrome
Fructose
Spinal Cord
Blood Pressure
topic Metabolic Syndrome
Fructose
Spinal Cord
Blood Pressure
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Blood pressure responses to intrathecal (i.t.) injection of neurochemicals were examined in the fructose-fed rat, an experimental model of metabolic syndrome.Sprague-Dawley rats receiving either tap water or water containing 10% fructose during 8 weeks were anesthetized with sodium pentobarbital. The endocannabinoid anandamide (100 nmol; i.t.) decreased mean blood pressure in control rats (21.276.3 mmHg), but had no effect in fructose-fed animals. Similarly, calcitonin generelated peptide (CGRP; 0.125 nmol; i.t.) decreased mean blood pressure in control, but not in treated rats. The high fructose diet did not cause significant changes in the pressor effects of i.t. administered noradrenaline (100 nmol) and N-methyl-D-aspartate (30 nmol). The nitric oxide donor sodium nitroprusside (500 nmol, i.t.) induced a brief hypotension followed by a sustained increase in mean blood pressure in control rats; however, this drug only produced pressor effects in fructose-fed animals. The GABAA-receptor agonist muscimol (8.8 nmol, i.t.) and the GABAB-receptor agonist baclofen (100 nmol, i.t.) decreased mean blood pressure 30–35 mmHg, both in control and in fructose-fed rats. Fructose potentiated the pressor effect of i.v. injected noradrenaline, but did not modify the hypotensive responses to i.v. administered sodium nitroprusside and acetylcholine.These results could suggest that, in pentobarbital-anesthetized rats, fructose feeding could alter spinal mechanisms of regulation of preganglionic sympathetic nerve activity. It is proposed that the spinal cord could be involved in the sympathetic dysfunction associated with the metabolic syndrome.
Fil: García, María del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina
Fil: Godoy, Yanina Cynthia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Celuch, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina
description Blood pressure responses to intrathecal (i.t.) injection of neurochemicals were examined in the fructose-fed rat, an experimental model of metabolic syndrome.Sprague-Dawley rats receiving either tap water or water containing 10% fructose during 8 weeks were anesthetized with sodium pentobarbital. The endocannabinoid anandamide (100 nmol; i.t.) decreased mean blood pressure in control rats (21.276.3 mmHg), but had no effect in fructose-fed animals. Similarly, calcitonin generelated peptide (CGRP; 0.125 nmol; i.t.) decreased mean blood pressure in control, but not in treated rats. The high fructose diet did not cause significant changes in the pressor effects of i.t. administered noradrenaline (100 nmol) and N-methyl-D-aspartate (30 nmol). The nitric oxide donor sodium nitroprusside (500 nmol, i.t.) induced a brief hypotension followed by a sustained increase in mean blood pressure in control rats; however, this drug only produced pressor effects in fructose-fed animals. The GABAA-receptor agonist muscimol (8.8 nmol, i.t.) and the GABAB-receptor agonist baclofen (100 nmol, i.t.) decreased mean blood pressure 30–35 mmHg, both in control and in fructose-fed rats. Fructose potentiated the pressor effect of i.v. injected noradrenaline, but did not modify the hypotensive responses to i.v. administered sodium nitroprusside and acetylcholine.These results could suggest that, in pentobarbital-anesthetized rats, fructose feeding could alter spinal mechanisms of regulation of preganglionic sympathetic nerve activity. It is proposed that the spinal cord could be involved in the sympathetic dysfunction associated with the metabolic syndrome.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12443
García, María del Carmen; Godoy, Yanina Cynthia; Celuch, Stella Maris; Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats; Elsevier Science; European Journal Of Pharmacology; 706; 1-3; 4-2013; 17-24
0014-2999
url http://hdl.handle.net/11336/12443
identifier_str_mv García, María del Carmen; Godoy, Yanina Cynthia; Celuch, Stella Maris; Impaired hypotensive responses induced by intrathecally injected drugs in fructose-fed rats; Elsevier Science; European Journal Of Pharmacology; 706; 1-3; 4-2013; 17-24
0014-2999
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2013.02.037
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014299913001465
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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