Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa
- Autores
- Lembo, Carina; Renna, Nicolas Federico; Diez, Emiliano Raúl; Vázquez Prieto, Marcela; Miatello, Roberto Miguel
- Año de publicación
- 2010
- Idioma
- español castellano
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Objective: To examine alterations in participation of endothelial progenitor cells (EPC) because of insulin resistance (IR) associated with an experimental model of metabolic syndrome (MS) generated by chronic administration of fructose to spontaneously hypertensive rats (SHR) Material and methods: WKY and SHR rats, male, were distributed into 4 groups (n = 8 c/u): WKY: control; FFR: WKY receiving fructose in drinking water to 10% (v/v) for 6 weeks , SHR; FFHR: SHR receiving fructose in drinking water to 10% (v/v) for 6 weeks. At the end of the protocol the following variables were determined: systolic blood pressure, biochemical variables, HOMA index, levels of EPC quantified by flow cytometry in peripheral blood and bone marrow, immunofluorescence in cell culture to identify markers CD34 and VEGFR-2, EPC colony count and NAD(P)H-oxidase activity in aortic tissue. Results: We confirmed the experimental model based on metabolic variables analyzed. A decrease in the levels of CPE, in peripheral blood and bone marrow, which becomes more important groups of animals treated with fructose was observed .In these groups there are also fewer colonies of developed EPC in cell culture and exhibit an increased levels of oxidative stress, estimated by the activity of NAD(P)H-oxidase. Conclusion: the SM caused by chronic administration of fructose in FFHR has proven to generate a decrease in the levels of CPE, as well as its functional capacity. The intracellular mechanisms that produce this phenomenon could be triggered by the degree of IR presented in this experimental model.
Objective: To examine alterations in participation of endothelial progenitor cells (EPC) because of insulin resistance (IR) associated with an experimental model of metabolic syndrome (MS) generated by chronic administration of fructose to spontaneously hypertensive rats (SHR) Material and methods: WKY and SHR rats, male, were distributed into 4 groups (n = 8 c/u): WKY: control; FFR: WKY receiving fructose in drinking water to 10% (v/v) for 6 weeks , SHR; FFHR: SHR receiving fructose in drinking water to 10% (v/v) for 6 weeks. At the end of the protocol the following variables were determined: systolic blood pressure, biochemical variables, HOMA index, levels of EPC quantified by flow cytometry in peripheral blood and bone marrow, immunofluorescence in cell culture to identify markers CD34 and VEGFR-2, EPC colony count and NAD(P)H-oxidase activity in aortic tissue. Results: We confirmed the experimental model based on metabolic variables analyzed. A decrease in the levels of CPE, in peripheral blood and bone marrow, which becomes more important groups of animals treated with fructose was observed .In these groups there are also fewer colonies of developed EPC in cell culture and exhibit an increased levels of oxidative stress, estimated by the activity of NAD(P)H-oxidase. Conclusion: the SM caused by chronic administration of fructose in FFHR has proven to generate a decrease in the levels of CPE, as well as its functional capacity. The intracellular mechanisms that produce this phenomenon could be triggered by the degree of IR presented in this experimental model.
Fil: Lembo, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Renna, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Vázquez Prieto, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Miatello, Roberto Miguel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina - Materia
-
Endothelial progenitor cells
Systolic blood pressure
Metabolic syndrome
Fructose-fed hypertensive rats - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/80580
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Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosaLembo, CarinaRenna, Nicolas FedericoDiez, Emiliano RaúlVázquez Prieto, MarcelaMiatello, Roberto MiguelEndothelial progenitor cellsSystolic blood pressureMetabolic syndromeFructose-fed hypertensive ratshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Objective: To examine alterations in participation of endothelial progenitor cells (EPC) because of insulin resistance (IR) associated with an experimental model of metabolic syndrome (MS) generated by chronic administration of fructose to spontaneously hypertensive rats (SHR) Material and methods: WKY and SHR rats, male, were distributed into 4 groups (n = 8 c/u): WKY: control; FFR: WKY receiving fructose in drinking water to 10% (v/v) for 6 weeks , SHR; FFHR: SHR receiving fructose in drinking water to 10% (v/v) for 6 weeks. At the end of the protocol the following variables were determined: systolic blood pressure, biochemical variables, HOMA index, levels of EPC quantified by flow cytometry in peripheral blood and bone marrow, immunofluorescence in cell culture to identify markers CD34 and VEGFR-2, EPC colony count and NAD(P)H-oxidase activity in aortic tissue. Results: We confirmed the experimental model based on metabolic variables analyzed. A decrease in the levels of CPE, in peripheral blood and bone marrow, which becomes more important groups of animals treated with fructose was observed .In these groups there are also fewer colonies of developed EPC in cell culture and exhibit an increased levels of oxidative stress, estimated by the activity of NAD(P)H-oxidase. Conclusion: the SM caused by chronic administration of fructose in FFHR has proven to generate a decrease in the levels of CPE, as well as its functional capacity. The intracellular mechanisms that produce this phenomenon could be triggered by the degree of IR presented in this experimental model.Objective: To examine alterations in participation of endothelial progenitor cells (EPC) because of insulin resistance (IR) associated with an experimental model of metabolic syndrome (MS) generated by chronic administration of fructose to spontaneously hypertensive rats (SHR) Material and methods: WKY and SHR rats, male, were distributed into 4 groups (n = 8 c/u): WKY: control; FFR: WKY receiving fructose in drinking water to 10% (v/v) for 6 weeks , SHR; FFHR: SHR receiving fructose in drinking water to 10% (v/v) for 6 weeks. At the end of the protocol the following variables were determined: systolic blood pressure, biochemical variables, HOMA index, levels of EPC quantified by flow cytometry in peripheral blood and bone marrow, immunofluorescence in cell culture to identify markers CD34 and VEGFR-2, EPC colony count and NAD(P)H-oxidase activity in aortic tissue. Results: We confirmed the experimental model based on metabolic variables analyzed. A decrease in the levels of CPE, in peripheral blood and bone marrow, which becomes more important groups of animals treated with fructose was observed .In these groups there are also fewer colonies of developed EPC in cell culture and exhibit an increased levels of oxidative stress, estimated by the activity of NAD(P)H-oxidase. Conclusion: the SM caused by chronic administration of fructose in FFHR has proven to generate a decrease in the levels of CPE, as well as its functional capacity. The intracellular mechanisms that produce this phenomenon could be triggered by the degree of IR presented in this experimental model.Fil: Lembo, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Renna, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Vázquez Prieto, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Miatello, Roberto Miguel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaUniversidad Nacional de Cuyo. Facultad de Ciencias Médicas2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80580Lembo, Carina; Renna, Nicolas Federico; Diez, Emiliano Raúl; Vázquez Prieto, Marcela; Miatello, Roberto Miguel; Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Revista Médica Universitaria; 6; 4; 12-2010; 1-181669-8991CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/http://bdigital.uncu.edu.ar/app/navegador/?idobjeto=3898info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:32Zoai:ri.conicet.gov.ar:11336/80580instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:32.883CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa |
title |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa |
spellingShingle |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa Lembo, Carina Endothelial progenitor cells Systolic blood pressure Metabolic syndrome Fructose-fed hypertensive rats |
title_short |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa |
title_full |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa |
title_fullStr |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa |
title_full_unstemmed |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa |
title_sort |
Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa |
dc.creator.none.fl_str_mv |
Lembo, Carina Renna, Nicolas Federico Diez, Emiliano Raúl Vázquez Prieto, Marcela Miatello, Roberto Miguel |
author |
Lembo, Carina |
author_facet |
Lembo, Carina Renna, Nicolas Federico Diez, Emiliano Raúl Vázquez Prieto, Marcela Miatello, Roberto Miguel |
author_role |
author |
author2 |
Renna, Nicolas Federico Diez, Emiliano Raúl Vázquez Prieto, Marcela Miatello, Roberto Miguel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Endothelial progenitor cells Systolic blood pressure Metabolic syndrome Fructose-fed hypertensive rats |
topic |
Endothelial progenitor cells Systolic blood pressure Metabolic syndrome Fructose-fed hypertensive rats |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Objective: To examine alterations in participation of endothelial progenitor cells (EPC) because of insulin resistance (IR) associated with an experimental model of metabolic syndrome (MS) generated by chronic administration of fructose to spontaneously hypertensive rats (SHR) Material and methods: WKY and SHR rats, male, were distributed into 4 groups (n = 8 c/u): WKY: control; FFR: WKY receiving fructose in drinking water to 10% (v/v) for 6 weeks , SHR; FFHR: SHR receiving fructose in drinking water to 10% (v/v) for 6 weeks. At the end of the protocol the following variables were determined: systolic blood pressure, biochemical variables, HOMA index, levels of EPC quantified by flow cytometry in peripheral blood and bone marrow, immunofluorescence in cell culture to identify markers CD34 and VEGFR-2, EPC colony count and NAD(P)H-oxidase activity in aortic tissue. Results: We confirmed the experimental model based on metabolic variables analyzed. A decrease in the levels of CPE, in peripheral blood and bone marrow, which becomes more important groups of animals treated with fructose was observed .In these groups there are also fewer colonies of developed EPC in cell culture and exhibit an increased levels of oxidative stress, estimated by the activity of NAD(P)H-oxidase. Conclusion: the SM caused by chronic administration of fructose in FFHR has proven to generate a decrease in the levels of CPE, as well as its functional capacity. The intracellular mechanisms that produce this phenomenon could be triggered by the degree of IR presented in this experimental model. Objective: To examine alterations in participation of endothelial progenitor cells (EPC) because of insulin resistance (IR) associated with an experimental model of metabolic syndrome (MS) generated by chronic administration of fructose to spontaneously hypertensive rats (SHR) Material and methods: WKY and SHR rats, male, were distributed into 4 groups (n = 8 c/u): WKY: control; FFR: WKY receiving fructose in drinking water to 10% (v/v) for 6 weeks , SHR; FFHR: SHR receiving fructose in drinking water to 10% (v/v) for 6 weeks. At the end of the protocol the following variables were determined: systolic blood pressure, biochemical variables, HOMA index, levels of EPC quantified by flow cytometry in peripheral blood and bone marrow, immunofluorescence in cell culture to identify markers CD34 and VEGFR-2, EPC colony count and NAD(P)H-oxidase activity in aortic tissue. Results: We confirmed the experimental model based on metabolic variables analyzed. A decrease in the levels of CPE, in peripheral blood and bone marrow, which becomes more important groups of animals treated with fructose was observed .In these groups there are also fewer colonies of developed EPC in cell culture and exhibit an increased levels of oxidative stress, estimated by the activity of NAD(P)H-oxidase. Conclusion: the SM caused by chronic administration of fructose in FFHR has proven to generate a decrease in the levels of CPE, as well as its functional capacity. The intracellular mechanisms that produce this phenomenon could be triggered by the degree of IR presented in this experimental model. Fil: Lembo, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Renna, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Vázquez Prieto, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Miatello, Roberto Miguel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina |
description |
Objective: To examine alterations in participation of endothelial progenitor cells (EPC) because of insulin resistance (IR) associated with an experimental model of metabolic syndrome (MS) generated by chronic administration of fructose to spontaneously hypertensive rats (SHR) Material and methods: WKY and SHR rats, male, were distributed into 4 groups (n = 8 c/u): WKY: control; FFR: WKY receiving fructose in drinking water to 10% (v/v) for 6 weeks , SHR; FFHR: SHR receiving fructose in drinking water to 10% (v/v) for 6 weeks. At the end of the protocol the following variables were determined: systolic blood pressure, biochemical variables, HOMA index, levels of EPC quantified by flow cytometry in peripheral blood and bone marrow, immunofluorescence in cell culture to identify markers CD34 and VEGFR-2, EPC colony count and NAD(P)H-oxidase activity in aortic tissue. Results: We confirmed the experimental model based on metabolic variables analyzed. A decrease in the levels of CPE, in peripheral blood and bone marrow, which becomes more important groups of animals treated with fructose was observed .In these groups there are also fewer colonies of developed EPC in cell culture and exhibit an increased levels of oxidative stress, estimated by the activity of NAD(P)H-oxidase. Conclusion: the SM caused by chronic administration of fructose in FFHR has proven to generate a decrease in the levels of CPE, as well as its functional capacity. The intracellular mechanisms that produce this phenomenon could be triggered by the degree of IR presented in this experimental model. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/80580 Lembo, Carina; Renna, Nicolas Federico; Diez, Emiliano Raúl; Vázquez Prieto, Marcela; Miatello, Roberto Miguel; Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Revista Médica Universitaria; 6; 4; 12-2010; 1-18 1669-8991 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/80580 |
identifier_str_mv |
Lembo, Carina; Renna, Nicolas Federico; Diez, Emiliano Raúl; Vázquez Prieto, Marcela; Miatello, Roberto Miguel; Caracterización y cuantificación de células progenitoras endoteliales de ratas espontáneamente hipertensas alimentadas con fructosa; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Revista Médica Universitaria; 6; 4; 12-2010; 1-18 1669-8991 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
spa |
language |
spa |
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info:eu-repo/semantics/altIdentifier/url/http://bdigital.uncu.edu.ar/app/navegador/?idobjeto=3898 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidad Nacional de Cuyo. Facultad de Ciencias Médicas |
publisher.none.fl_str_mv |
Universidad Nacional de Cuyo. Facultad de Ciencias Médicas |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |