Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model

Autores
Grillo, Claudia Alejandra; Dulout, Fernando Noel; Güerci, Alba Mabel
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: The present study was designed to evaluate the effects of sequential exposures to low doses of gamma-radiation that induce a radioadaptive response to a later high-dose of radiation in CHO-K1 cells. Materials and methods: Cells were cultured in four dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 0.1 Gy gamma-rays. After the last radiation period (chronic radiation) the culture was irradiated with a higher dose (1 Gy). Each cell culture was immediately divided into two fractions: one of them was used to carry out the comet assay and the other for the structural chromosome aberration test. In the first fraction, genotoxic damage was evaluated by degree of damage in 300 cells per experimental point. The second assay was performed with 400 cells per treatment. The statistical analysis was carried out using the 2-test. Results: Results from these assays demonstrated a genotoxic effect for both the adaptive and acute treatments (p0.001). The comet assay showed a significant increase in damage for the combined treatment when compared with 1 Gy treatment (p0.001). The frequency of chromosomal aberrations (CA) was lower for the combined treatment than for that using the highest radiation dose. Conclusions: These results suggest the possible induction of a radioadaptive response after the sequential exposure to very low doses of radiation. The finding of decreased cytogenetic damage after one cell cycle and not immediately after radiation could indicate the eventual potentiation of repair mechanisms.
Fil: Grillo, Claudia Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dulout, Fernando Noel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Güerci, Alba Mabel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Comet Assay
Radioadaptive Response
Structural Chromosome Aberration Test
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82168
Acceder
id CONICETDig_d914c508aff45a97306ea5e104aaff9d
oai_identifier_str oai:ri.conicet.gov.ar:11336/82168
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional modelGrillo, Claudia AlejandraDulout, Fernando NoelGüerci, Alba MabelComet AssayRadioadaptive ResponseStructural Chromosome Aberration Testhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Purpose: The present study was designed to evaluate the effects of sequential exposures to low doses of gamma-radiation that induce a radioadaptive response to a later high-dose of radiation in CHO-K1 cells. Materials and methods: Cells were cultured in four dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 0.1 Gy gamma-rays. After the last radiation period (chronic radiation) the culture was irradiated with a higher dose (1 Gy). Each cell culture was immediately divided into two fractions: one of them was used to carry out the comet assay and the other for the structural chromosome aberration test. In the first fraction, genotoxic damage was evaluated by degree of damage in 300 cells per experimental point. The second assay was performed with 400 cells per treatment. The statistical analysis was carried out using the 2-test. Results: Results from these assays demonstrated a genotoxic effect for both the adaptive and acute treatments (p0.001). The comet assay showed a significant increase in damage for the combined treatment when compared with 1 Gy treatment (p0.001). The frequency of chromosomal aberrations (CA) was lower for the combined treatment than for that using the highest radiation dose. Conclusions: These results suggest the possible induction of a radioadaptive response after the sequential exposure to very low doses of radiation. The finding of decreased cytogenetic damage after one cell cycle and not immediately after radiation could indicate the eventual potentiation of repair mechanisms.Fil: Grillo, Claudia Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dulout, Fernando Noel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Güerci, Alba Mabel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82168Grillo, Claudia Alejandra; Dulout, Fernando Noel; Güerci, Alba Mabel; Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model; Taylor & Francis; International Journal Of Radiation Biology; 85; 2; 2-2009; 159-1660955-30021362-3095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/09553000902740143info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09553000902740143info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:53Zoai:ri.conicet.gov.ar:11336/82168instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:54.087CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
title Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
spellingShingle Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
Grillo, Claudia Alejandra
Comet Assay
Radioadaptive Response
Structural Chromosome Aberration Test
title_short Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
title_full Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
title_fullStr Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
title_full_unstemmed Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
title_sort Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
dc.creator.none.fl_str_mv Grillo, Claudia Alejandra
Dulout, Fernando Noel
Güerci, Alba Mabel
author Grillo, Claudia Alejandra
author_facet Grillo, Claudia Alejandra
Dulout, Fernando Noel
Güerci, Alba Mabel
author_role author
author2 Dulout, Fernando Noel
Güerci, Alba Mabel
author2_role author
author
dc.subject.none.fl_str_mv Comet Assay
Radioadaptive Response
Structural Chromosome Aberration Test
topic Comet Assay
Radioadaptive Response
Structural Chromosome Aberration Test
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Purpose: The present study was designed to evaluate the effects of sequential exposures to low doses of gamma-radiation that induce a radioadaptive response to a later high-dose of radiation in CHO-K1 cells. Materials and methods: Cells were cultured in four dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 0.1 Gy gamma-rays. After the last radiation period (chronic radiation) the culture was irradiated with a higher dose (1 Gy). Each cell culture was immediately divided into two fractions: one of them was used to carry out the comet assay and the other for the structural chromosome aberration test. In the first fraction, genotoxic damage was evaluated by degree of damage in 300 cells per experimental point. The second assay was performed with 400 cells per treatment. The statistical analysis was carried out using the 2-test. Results: Results from these assays demonstrated a genotoxic effect for both the adaptive and acute treatments (p0.001). The comet assay showed a significant increase in damage for the combined treatment when compared with 1 Gy treatment (p0.001). The frequency of chromosomal aberrations (CA) was lower for the combined treatment than for that using the highest radiation dose. Conclusions: These results suggest the possible induction of a radioadaptive response after the sequential exposure to very low doses of radiation. The finding of decreased cytogenetic damage after one cell cycle and not immediately after radiation could indicate the eventual potentiation of repair mechanisms.
Fil: Grillo, Claudia Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dulout, Fernando Noel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Güerci, Alba Mabel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Purpose: The present study was designed to evaluate the effects of sequential exposures to low doses of gamma-radiation that induce a radioadaptive response to a later high-dose of radiation in CHO-K1 cells. Materials and methods: Cells were cultured in four dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 0.1 Gy gamma-rays. After the last radiation period (chronic radiation) the culture was irradiated with a higher dose (1 Gy). Each cell culture was immediately divided into two fractions: one of them was used to carry out the comet assay and the other for the structural chromosome aberration test. In the first fraction, genotoxic damage was evaluated by degree of damage in 300 cells per experimental point. The second assay was performed with 400 cells per treatment. The statistical analysis was carried out using the 2-test. Results: Results from these assays demonstrated a genotoxic effect for both the adaptive and acute treatments (p0.001). The comet assay showed a significant increase in damage for the combined treatment when compared with 1 Gy treatment (p0.001). The frequency of chromosomal aberrations (CA) was lower for the combined treatment than for that using the highest radiation dose. Conclusions: These results suggest the possible induction of a radioadaptive response after the sequential exposure to very low doses of radiation. The finding of decreased cytogenetic damage after one cell cycle and not immediately after radiation could indicate the eventual potentiation of repair mechanisms.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82168
Grillo, Claudia Alejandra; Dulout, Fernando Noel; Güerci, Alba Mabel; Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model; Taylor & Francis; International Journal Of Radiation Biology; 85; 2; 2-2009; 159-166
0955-3002
1362-3095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82168
identifier_str_mv Grillo, Claudia Alejandra; Dulout, Fernando Noel; Güerci, Alba Mabel; Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model; Taylor & Francis; International Journal Of Radiation Biology; 85; 2; 2-2009; 159-166
0955-3002
1362-3095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/09553000902740143
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09553000902740143
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613682891325440
score 13.070432

Publicaciones similares