Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity

Autores
Padula, Gisel; Ponzinibbio, Maria Virginia; Picco, Sebastian Julio; Seoane, Analia Isabel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Amitraz is a formamidine widely used in Veterinary Medicine for the treatment of ectoparasites. It is a highly liposoluble compound that is quickly absorbed through the skin and mucous membranes, thus making exposure potentially dangerous for humans and animals. The aim of this study was to compare the genotoxic potential of the active constituent of the insecticide amitraz and a commercial product containing amitraz in vitro in For Peer Review Only hamster cells. The induction of primary DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay) and the apoptotic ability was examined by the Annexin V/PI staining assay. The commercial formulation significantly increased the index of DNA damage at concentrations of 2.50-3.75 µg/ml compared to the control. The active constituent only induced significant DNA damage with the highest concentration (3.75 µg/ml). Although both tested products increased the frequency of cell death, neither of them induced significant differences. Genotoxic potential is a primary risk factor for long-term effects such as carcinogenic and reproductive toxicology. Results presented here highlight the need for further investigation of the potential health risk of this veterinary medicine.
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Picco, Sebastian Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
AMITRAZ
COMET ASSAY
APOPTOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268850
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268850
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicityPadula, GiselPonzinibbio, Maria VirginiaPicco, Sebastian JulioSeoane, Analia IsabelAMITRAZCOMET ASSAYAPOPTOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Amitraz is a formamidine widely used in Veterinary Medicine for the treatment of ectoparasites. It is a highly liposoluble compound that is quickly absorbed through the skin and mucous membranes, thus making exposure potentially dangerous for humans and animals. The aim of this study was to compare the genotoxic potential of the active constituent of the insecticide amitraz and a commercial product containing amitraz in vitro in For Peer Review Only hamster cells. The induction of primary DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay) and the apoptotic ability was examined by the Annexin V/PI staining assay. The commercial formulation significantly increased the index of DNA damage at concentrations of 2.50-3.75 µg/ml compared to the control. The active constituent only induced significant DNA damage with the highest concentration (3.75 µg/ml). Although both tested products increased the frequency of cell death, neither of them induced significant differences. Genotoxic potential is a primary risk factor for long-term effects such as carcinogenic and reproductive toxicology. Results presented here highlight the need for further investigation of the potential health risk of this veterinary medicine.Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Picco, Sebastian Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaTaylor & Francis2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268850Padula, Gisel; Ponzinibbio, Maria Virginia; Picco, Sebastian Julio; Seoane, Analia Isabel; Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity; Taylor & Francis; Toxicology Mechanisms and Methods; 22; 9; 11-2012; 657-6611537-6524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/15376516.2012.666683info:eu-repo/semantics/altIdentifier/doi/10.3109/15376516.2012.666683info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:39Zoai:ri.conicet.gov.ar:11336/268850instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:39.441CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
title Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
spellingShingle Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
Padula, Gisel
AMITRAZ
COMET ASSAY
APOPTOSIS
title_short Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
title_full Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
title_fullStr Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
title_full_unstemmed Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
title_sort Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity
dc.creator.none.fl_str_mv Padula, Gisel
Ponzinibbio, Maria Virginia
Picco, Sebastian Julio
Seoane, Analia Isabel
author Padula, Gisel
author_facet Padula, Gisel
Ponzinibbio, Maria Virginia
Picco, Sebastian Julio
Seoane, Analia Isabel
author_role author
author2 Ponzinibbio, Maria Virginia
Picco, Sebastian Julio
Seoane, Analia Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv AMITRAZ
COMET ASSAY
APOPTOSIS
topic AMITRAZ
COMET ASSAY
APOPTOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Amitraz is a formamidine widely used in Veterinary Medicine for the treatment of ectoparasites. It is a highly liposoluble compound that is quickly absorbed through the skin and mucous membranes, thus making exposure potentially dangerous for humans and animals. The aim of this study was to compare the genotoxic potential of the active constituent of the insecticide amitraz and a commercial product containing amitraz in vitro in For Peer Review Only hamster cells. The induction of primary DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay) and the apoptotic ability was examined by the Annexin V/PI staining assay. The commercial formulation significantly increased the index of DNA damage at concentrations of 2.50-3.75 µg/ml compared to the control. The active constituent only induced significant DNA damage with the highest concentration (3.75 µg/ml). Although both tested products increased the frequency of cell death, neither of them induced significant differences. Genotoxic potential is a primary risk factor for long-term effects such as carcinogenic and reproductive toxicology. Results presented here highlight the need for further investigation of the potential health risk of this veterinary medicine.
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Picco, Sebastian Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Amitraz is a formamidine widely used in Veterinary Medicine for the treatment of ectoparasites. It is a highly liposoluble compound that is quickly absorbed through the skin and mucous membranes, thus making exposure potentially dangerous for humans and animals. The aim of this study was to compare the genotoxic potential of the active constituent of the insecticide amitraz and a commercial product containing amitraz in vitro in For Peer Review Only hamster cells. The induction of primary DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay) and the apoptotic ability was examined by the Annexin V/PI staining assay. The commercial formulation significantly increased the index of DNA damage at concentrations of 2.50-3.75 µg/ml compared to the control. The active constituent only induced significant DNA damage with the highest concentration (3.75 µg/ml). Although both tested products increased the frequency of cell death, neither of them induced significant differences. Genotoxic potential is a primary risk factor for long-term effects such as carcinogenic and reproductive toxicology. Results presented here highlight the need for further investigation of the potential health risk of this veterinary medicine.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268850
Padula, Gisel; Ponzinibbio, Maria Virginia; Picco, Sebastian Julio; Seoane, Analia Isabel; Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity; Taylor & Francis; Toxicology Mechanisms and Methods; 22; 9; 11-2012; 657-661
1537-6524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268850
identifier_str_mv Padula, Gisel; Ponzinibbio, Maria Virginia; Picco, Sebastian Julio; Seoane, Analia Isabel; Assessment of the adverse effects of the acaricide amitraz: in vitro evaluation of genotoxicity; Taylor & Francis; Toxicology Mechanisms and Methods; 22; 9; 11-2012; 657-661
1537-6524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/15376516.2012.666683
info:eu-repo/semantics/altIdentifier/doi/10.3109/15376516.2012.666683
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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