Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide

Autores
Leveroni, Flavia Antonela; Caffetti, Jacqueline Diana; Pastori, María Cristina
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of pesticides may affect the aquatic ecosystem. Through soil, these contaminants are drained into rivers. In South America, glyphosate-base herbicides, such as Roundup, are widely used in agricultural activities. In this work, the genotoxic effects of Roundup Full II® were evaluated in blood, gill and liver cells of Piaractus mesopotamicus, species of great ecologicaland commercial value in South America. Previously, the lethal concentration 50 (LC50) for 96 h of exposure was determined, with a theoretical value of 8.92 mg l?1. Genotoxic analysis in cells of the three tissues was performed by the comet, micronuclei (MN) and nuclear abnormalities (NA) assays after 96 h exposing fish to a sub-lethal concentration of Roundup Full II® (2.75 mg l?1).Comet assay results for the three tissues showed a significantly greater damage compared to the negative control and no statistical differences were observed between tissues responses. For MN and NA significantly higher frequencies were also demonstrated, indicating diverse kinds of damage like clastogenic events in the three tissues, with gills being the most sensitive one.
Fil: Leveroni, Flavia Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Caffetti, Jacqueline Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Pastori, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Materia
Genotoxicity
Comet Assay
Micronucleus Test
Lc50
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41128
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41128
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicideLeveroni, Flavia AntonelaCaffetti, Jacqueline DianaPastori, María CristinaGenotoxicityComet AssayMicronucleus TestLc50https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The use of pesticides may affect the aquatic ecosystem. Through soil, these contaminants are drained into rivers. In South America, glyphosate-base herbicides, such as Roundup, are widely used in agricultural activities. In this work, the genotoxic effects of Roundup Full II® were evaluated in blood, gill and liver cells of Piaractus mesopotamicus, species of great ecologicaland commercial value in South America. Previously, the lethal concentration 50 (LC50) for 96 h of exposure was determined, with a theoretical value of 8.92 mg l?1. Genotoxic analysis in cells of the three tissues was performed by the comet, micronuclei (MN) and nuclear abnormalities (NA) assays after 96 h exposing fish to a sub-lethal concentration of Roundup Full II® (2.75 mg l?1).Comet assay results for the three tissues showed a significantly greater damage compared to the negative control and no statistical differences were observed between tissues responses. For MN and NA significantly higher frequencies were also demonstrated, indicating diverse kinds of damage like clastogenic events in the three tissues, with gills being the most sensitive one.Fil: Leveroni, Flavia Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Caffetti, Jacqueline Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Pastori, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaUniv Florence Botany Inst2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41128Leveroni, Flavia Antonela; Caffetti, Jacqueline Diana; Pastori, María Cristina; Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide; Univ Florence Botany Inst; Caryologia; 70; 1; 11-2016; 21-280008-71142165-5391CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1080/00087114.2016.1254454info:eu-repo/semantics/altIdentifier/doi/10.1080/00087114.2016.1254454info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:07Zoai:ri.conicet.gov.ar:11336/41128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:07.36CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
spellingShingle Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
Leveroni, Flavia Antonela
Genotoxicity
Comet Assay
Micronucleus Test
Lc50
title_short Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_full Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_fullStr Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_full_unstemmed Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_sort Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
dc.creator.none.fl_str_mv Leveroni, Flavia Antonela
Caffetti, Jacqueline Diana
Pastori, María Cristina
author Leveroni, Flavia Antonela
author_facet Leveroni, Flavia Antonela
Caffetti, Jacqueline Diana
Pastori, María Cristina
author_role author
author2 Caffetti, Jacqueline Diana
Pastori, María Cristina
author2_role author
author
dc.subject.none.fl_str_mv Genotoxicity
Comet Assay
Micronucleus Test
Lc50
topic Genotoxicity
Comet Assay
Micronucleus Test
Lc50
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of pesticides may affect the aquatic ecosystem. Through soil, these contaminants are drained into rivers. In South America, glyphosate-base herbicides, such as Roundup, are widely used in agricultural activities. In this work, the genotoxic effects of Roundup Full II® were evaluated in blood, gill and liver cells of Piaractus mesopotamicus, species of great ecologicaland commercial value in South America. Previously, the lethal concentration 50 (LC50) for 96 h of exposure was determined, with a theoretical value of 8.92 mg l?1. Genotoxic analysis in cells of the three tissues was performed by the comet, micronuclei (MN) and nuclear abnormalities (NA) assays after 96 h exposing fish to a sub-lethal concentration of Roundup Full II® (2.75 mg l?1).Comet assay results for the three tissues showed a significantly greater damage compared to the negative control and no statistical differences were observed between tissues responses. For MN and NA significantly higher frequencies were also demonstrated, indicating diverse kinds of damage like clastogenic events in the three tissues, with gills being the most sensitive one.
Fil: Leveroni, Flavia Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Caffetti, Jacqueline Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Pastori, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
description The use of pesticides may affect the aquatic ecosystem. Through soil, these contaminants are drained into rivers. In South America, glyphosate-base herbicides, such as Roundup, are widely used in agricultural activities. In this work, the genotoxic effects of Roundup Full II® were evaluated in blood, gill and liver cells of Piaractus mesopotamicus, species of great ecologicaland commercial value in South America. Previously, the lethal concentration 50 (LC50) for 96 h of exposure was determined, with a theoretical value of 8.92 mg l?1. Genotoxic analysis in cells of the three tissues was performed by the comet, micronuclei (MN) and nuclear abnormalities (NA) assays after 96 h exposing fish to a sub-lethal concentration of Roundup Full II® (2.75 mg l?1).Comet assay results for the three tissues showed a significantly greater damage compared to the negative control and no statistical differences were observed between tissues responses. For MN and NA significantly higher frequencies were also demonstrated, indicating diverse kinds of damage like clastogenic events in the three tissues, with gills being the most sensitive one.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41128
Leveroni, Flavia Antonela; Caffetti, Jacqueline Diana; Pastori, María Cristina; Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide; Univ Florence Botany Inst; Caryologia; 70; 1; 11-2016; 21-28
0008-7114
2165-5391
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41128
identifier_str_mv Leveroni, Flavia Antonela; Caffetti, Jacqueline Diana; Pastori, María Cristina; Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide; Univ Florence Botany Inst; Caryologia; 70; 1; 11-2016; 21-28
0008-7114
2165-5391
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.1080/00087114.2016.1254454
info:eu-repo/semantics/altIdentifier/doi/10.1080/00087114.2016.1254454
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Univ Florence Botany Inst
publisher.none.fl_str_mv Univ Florence Botany Inst
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268954509180928
score 13.13397

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