Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide

Autores
Leveroni, Flavia Antonela; Caffetti, Jacqueline Diana; Pastori, María Cristina
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Leveroni, Flavia Antonela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.
Fil: Leveroni, Flavia Antonela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.
Fil: Leveroni, Flavia Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.
Fil: Caffetti, Jacqueline Diana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.
Fil: Caffetti, Jacqueline Diana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.
Fil: Caffetti, Jacqueline Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.
Fil: Pastori, María Cristina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.
Fil: Pastori, María Cristina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.
Fil: Pastori, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.
The use of pesticides may affect the aquatic ecosystem. Through soil, these contaminants are drained into rivers. In South America, glyphosate-base herbicides, such as Roundup, are widely used in agricultural activities. In this work, the genotoxic effects of Roundup Full II® were evaluated in blood, gill and liver cells of Piaractus mesopotamicus, species of great ecological and commercial value in South America. Previously, the lethal concentration 50 (LC50) for 96 h of exposure was determined, with a theoretical value of 8.92 mg l–1. Genotoxic analysis in cells of the three tissues was performed by the comet, micronuclei (MN) and nuclear abnormalities (NA) assays after 96 h exposing fish to a sub-lethal concentration of Roundup Full II® (2.75 mg l–1). Comet assay results for the three tissues showed a significantly greater damage compared to the negative control and no statistical differences were observed between tissues responses. For MN and NA significantly higher frequencies were also demonstrated, indicating diverse kinds of damage like clastogenic events in the three tissues, with gills being the most sensitive one.
Materia
Genotoxicity
Comet assay
Micronucleus test
Nuclear abnormalities
LC50
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Repositorio
Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
Institución
Universidad Nacional de Misiones
OAI Identificador
oai:rid.unam.edu.ar:20.500.12219/4337
Acceder
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spelling Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicideLeveroni, Flavia AntonelaCaffetti, Jacqueline DianaPastori, María CristinaGenotoxicityComet assayMicronucleus testNuclear abnormalitiesLC50Fil: Leveroni, Flavia Antonela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.Fil: Leveroni, Flavia Antonela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.Fil: Leveroni, Flavia Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.Fil: Caffetti, Jacqueline Diana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.Fil: Caffetti, Jacqueline Diana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.Fil: Caffetti, Jacqueline Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.Fil: Pastori, María Cristina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.Fil: Pastori, María Cristina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.Fil: Pastori, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.The use of pesticides may affect the aquatic ecosystem. Through soil, these contaminants are drained into rivers. In South America, glyphosate-base herbicides, such as Roundup, are widely used in agricultural activities. In this work, the genotoxic effects of Roundup Full II® were evaluated in blood, gill and liver cells of Piaractus mesopotamicus, species of great ecological and commercial value in South America. Previously, the lethal concentration 50 (LC50) for 96 h of exposure was determined, with a theoretical value of 8.92 mg l–1. Genotoxic analysis in cells of the three tissues was performed by the comet, micronuclei (MN) and nuclear abnormalities (NA) assays after 96 h exposing fish to a sub-lethal concentration of Roundup Full II® (2.75 mg l–1). Comet assay results for the three tissues showed a significantly greater damage compared to the negative control and no statistical differences were observed between tissues responses. For MN and NA significantly higher frequencies were also demonstrated, indicating diverse kinds of damage like clastogenic events in the three tissues, with gills being the most sensitive one.Taylor & Francis2016-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdf934.1 KBhttps://hdl.handle.net/20.500.12219/4337enginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/00087114.2016.1254454info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)instname:Universidad Nacional de Misiones2025-09-04T11:43:13Zoai:rid.unam.edu.ar:20.500.12219/4337instacron:UNAMInstitucionalhttps://rid.unam.edu.ar/Universidad públicahttps://www.unam.edu.ar/https://rid.unam.edu.ar/oai/rsnrdArgentinaopendoar:2025-09-04 11:43:13.389Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM) - Universidad Nacional de Misionesfalse
dc.title.none.fl_str_mv Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
spellingShingle Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
Leveroni, Flavia Antonela
Genotoxicity
Comet assay
Micronucleus test
Nuclear abnormalities
LC50
title_short Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_full Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_fullStr Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_full_unstemmed Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
title_sort Genotoxic response of blood, gill and liver cells of Piaractus mesopotamicus after an acute exposure to a glyphosate-based herbicide
dc.creator.none.fl_str_mv Leveroni, Flavia Antonela
Caffetti, Jacqueline Diana
Pastori, María Cristina
author Leveroni, Flavia Antonela
author_facet Leveroni, Flavia Antonela
Caffetti, Jacqueline Diana
Pastori, María Cristina
author_role author
author2 Caffetti, Jacqueline Diana
Pastori, María Cristina
author2_role author
author
dc.subject.none.fl_str_mv Genotoxicity
Comet assay
Micronucleus test
Nuclear abnormalities
LC50
topic Genotoxicity
Comet assay
Micronucleus test
Nuclear abnormalities
LC50
dc.description.none.fl_txt_mv Fil: Leveroni, Flavia Antonela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.
Fil: Leveroni, Flavia Antonela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.
Fil: Leveroni, Flavia Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.
Fil: Caffetti, Jacqueline Diana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.
Fil: Caffetti, Jacqueline Diana. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.
Fil: Caffetti, Jacqueline Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.
Fil: Pastori, María Cristina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.
Fil: Pastori, María Cristina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical; Argentina.
Fil: Pastori, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Nordeste). Instituto de Biología Subtropical; Argentina.
The use of pesticides may affect the aquatic ecosystem. Through soil, these contaminants are drained into rivers. In South America, glyphosate-base herbicides, such as Roundup, are widely used in agricultural activities. In this work, the genotoxic effects of Roundup Full II® were evaluated in blood, gill and liver cells of Piaractus mesopotamicus, species of great ecological and commercial value in South America. Previously, the lethal concentration 50 (LC50) for 96 h of exposure was determined, with a theoretical value of 8.92 mg l–1. Genotoxic analysis in cells of the three tissues was performed by the comet, micronuclei (MN) and nuclear abnormalities (NA) assays after 96 h exposing fish to a sub-lethal concentration of Roundup Full II® (2.75 mg l–1). Comet assay results for the three tissues showed a significantly greater damage compared to the negative control and no statistical differences were observed between tissues responses. For MN and NA significantly higher frequencies were also demonstrated, indicating diverse kinds of damage like clastogenic events in the three tissues, with gills being the most sensitive one.
description Fil: Leveroni, Flavia Antonela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Citogenética General y Monitoreo Ambiental; Argentina.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12219/4337
url https://hdl.handle.net/20.500.12219/4337
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/00087114.2016.1254454
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 4.0 Internacional
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dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
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