Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests

Autores
Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, Irma Alicia; Ugnia, Laura Inés; Gonzalez Cid, Marcela Beatriz; Larripa, Irene Beatriz; Gorla, Nora Bibiana Maria
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It was evaluated the genotoxicity of glyphosate which up to now has heterogeneous results. The comet assay was performed in Hep-2 cells. The level of DNA damage in the control group (5.42 ± 1.83 arbitrary units) for tail moment (TM) measurements has shown a significant increase (p < 0.01) with glyphosate at a range concentration from 3.00 to 7.50 mM. In the chromosome aberrations (CA) test in human lymphocytes the herbicide (0.20-6.00 mM) showed no significant effects in comparison with the control group. In vivo, the micronucleus test (MNT) was evaluated in mice at three doses rendering statistical significant increases at 400 mg/kg (13.0 ± 3.08 micronucleated erythrocytes/1000 cells, p < 0.01). In the present study glyphosate was genotoxic in the comet assay in Hep-2 cells and in the MNT test at 400 mg/kg in mice. Thiobarbituric acid reactive substances (TBARs) levels, superoxide dismutase (SOD) and catalase (CAT) activities were quantified in their organs. The results showed an increase in these enzyme activities. © 2009 Elsevier B.V. All rights reserved.
Fil: Mañas, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Peralta, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Raviolo, Jose Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Garcia Ovando, Hugo. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Weyers, Irma Alicia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Ugnia, Laura Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Gonzalez Cid, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Materia
Catalase
Chromosome Aberrations
Comet Assay
Glyphosate
Micronucleus
Sod
Tbars
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54803
Acceder
id CONICETDig_6004cd9ffeab7701579d73659900c95a
oai_identifier_str oai:ri.conicet.gov.ar:11336/54803
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotoxicity of glyphosate assessed by the comet assay and cytogenetic testsMañas, Fernando JavierPeralta, LauraRaviolo, Jose MariaGarcia Ovando, HugoWeyers, Irma AliciaUgnia, Laura InésGonzalez Cid, Marcela BeatrizLarripa, Irene BeatrizGorla, Nora Bibiana MariaCatalaseChromosome AberrationsComet AssayGlyphosateMicronucleusSodTbarshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It was evaluated the genotoxicity of glyphosate which up to now has heterogeneous results. The comet assay was performed in Hep-2 cells. The level of DNA damage in the control group (5.42 ± 1.83 arbitrary units) for tail moment (TM) measurements has shown a significant increase (p < 0.01) with glyphosate at a range concentration from 3.00 to 7.50 mM. In the chromosome aberrations (CA) test in human lymphocytes the herbicide (0.20-6.00 mM) showed no significant effects in comparison with the control group. In vivo, the micronucleus test (MNT) was evaluated in mice at three doses rendering statistical significant increases at 400 mg/kg (13.0 ± 3.08 micronucleated erythrocytes/1000 cells, p < 0.01). In the present study glyphosate was genotoxic in the comet assay in Hep-2 cells and in the MNT test at 400 mg/kg in mice. Thiobarbituric acid reactive substances (TBARs) levels, superoxide dismutase (SOD) and catalase (CAT) activities were quantified in their organs. The results showed an increase in these enzyme activities. © 2009 Elsevier B.V. All rights reserved.Fil: Mañas, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Peralta, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Raviolo, Jose Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Garcia Ovando, Hugo. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Weyers, Irma Alicia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Ugnia, Laura Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Gonzalez Cid, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaElsevier Science2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54803Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, Irma Alicia; et al.; Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests; Elsevier Science; Environmental Toxicology and Pharmacology; 28; 1; 7-2009; 37-411382-6689CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.etap.2009.02.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1382668909000258info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:17Zoai:ri.conicet.gov.ar:11336/54803instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:17.693CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
title Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
spellingShingle Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
Mañas, Fernando Javier
Catalase
Chromosome Aberrations
Comet Assay
Glyphosate
Micronucleus
Sod
Tbars
title_short Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
title_full Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
title_fullStr Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
title_full_unstemmed Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
title_sort Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
dc.creator.none.fl_str_mv Mañas, Fernando Javier
Peralta, Laura
Raviolo, Jose Maria
Garcia Ovando, Hugo
Weyers, Irma Alicia
Ugnia, Laura Inés
Gonzalez Cid, Marcela Beatriz
Larripa, Irene Beatriz
Gorla, Nora Bibiana Maria
author Mañas, Fernando Javier
author_facet Mañas, Fernando Javier
Peralta, Laura
Raviolo, Jose Maria
Garcia Ovando, Hugo
Weyers, Irma Alicia
Ugnia, Laura Inés
Gonzalez Cid, Marcela Beatriz
Larripa, Irene Beatriz
Gorla, Nora Bibiana Maria
author_role author
author2 Peralta, Laura
Raviolo, Jose Maria
Garcia Ovando, Hugo
Weyers, Irma Alicia
Ugnia, Laura Inés
Gonzalez Cid, Marcela Beatriz
Larripa, Irene Beatriz
Gorla, Nora Bibiana Maria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Catalase
Chromosome Aberrations
Comet Assay
Glyphosate
Micronucleus
Sod
Tbars
topic Catalase
Chromosome Aberrations
Comet Assay
Glyphosate
Micronucleus
Sod
Tbars
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It was evaluated the genotoxicity of glyphosate which up to now has heterogeneous results. The comet assay was performed in Hep-2 cells. The level of DNA damage in the control group (5.42 ± 1.83 arbitrary units) for tail moment (TM) measurements has shown a significant increase (p < 0.01) with glyphosate at a range concentration from 3.00 to 7.50 mM. In the chromosome aberrations (CA) test in human lymphocytes the herbicide (0.20-6.00 mM) showed no significant effects in comparison with the control group. In vivo, the micronucleus test (MNT) was evaluated in mice at three doses rendering statistical significant increases at 400 mg/kg (13.0 ± 3.08 micronucleated erythrocytes/1000 cells, p < 0.01). In the present study glyphosate was genotoxic in the comet assay in Hep-2 cells and in the MNT test at 400 mg/kg in mice. Thiobarbituric acid reactive substances (TBARs) levels, superoxide dismutase (SOD) and catalase (CAT) activities were quantified in their organs. The results showed an increase in these enzyme activities. © 2009 Elsevier B.V. All rights reserved.
Fil: Mañas, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Peralta, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Raviolo, Jose Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Garcia Ovando, Hugo. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Weyers, Irma Alicia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Ugnia, Laura Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Gonzalez Cid, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
description It was evaluated the genotoxicity of glyphosate which up to now has heterogeneous results. The comet assay was performed in Hep-2 cells. The level of DNA damage in the control group (5.42 ± 1.83 arbitrary units) for tail moment (TM) measurements has shown a significant increase (p < 0.01) with glyphosate at a range concentration from 3.00 to 7.50 mM. In the chromosome aberrations (CA) test in human lymphocytes the herbicide (0.20-6.00 mM) showed no significant effects in comparison with the control group. In vivo, the micronucleus test (MNT) was evaluated in mice at three doses rendering statistical significant increases at 400 mg/kg (13.0 ± 3.08 micronucleated erythrocytes/1000 cells, p < 0.01). In the present study glyphosate was genotoxic in the comet assay in Hep-2 cells and in the MNT test at 400 mg/kg in mice. Thiobarbituric acid reactive substances (TBARs) levels, superoxide dismutase (SOD) and catalase (CAT) activities were quantified in their organs. The results showed an increase in these enzyme activities. © 2009 Elsevier B.V. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54803
Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, Irma Alicia; et al.; Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests; Elsevier Science; Environmental Toxicology and Pharmacology; 28; 1; 7-2009; 37-41
1382-6689
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54803
identifier_str_mv Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, Irma Alicia; et al.; Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests; Elsevier Science; Environmental Toxicology and Pharmacology; 28; 1; 7-2009; 37-41
1382-6689
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.etap.2009.02.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1382668909000258
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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