Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina

Autores
Isaac, Paula; Martínez, Fabiana Lilian; Bourguignon, Natalia; Sanchez, Leandro Arturo; Ferrero, Marcela Alejandra
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Defined mixed cultures consisting of Pseudomonas and actinobacteria strains showed interesting polycyclic aromatic hydrocarbons (PAHs) removal values, improving greatly removal efficiency of strains in pure culture. These bacterial mixed cultures were synthesized with bacteria isolated from contaminated sites, previously evaluated regarding of naphthalene, phenantrene and pyrene removal when operating at flask scale. The results had shown that monocultures of Pseudomonas monteilii P26 and Pseudomonas sp. N3 could degrade efficiently low molecular weight (LMW) PAHs but they did not show interesting high molecular weight (HMW) PAHs removal capabilities. On the other hand, the actinobacteria Rhodococcus sp. P18, Gordonia sp. H19 and Rhodococcus sp. F27 were able to degrade relatively efficiently HMW PAHs but they did not remove LMW PAHs from culture medium. The combination of four of these five strains (called C15 mixed culture) removed 100 % of naphthalene and phenanthrene, and showed the highest pyrene biodegradation activity with removal values close to 42 %, almost 6-times higher than those values recorded with the strains in pure culture. In addition, this defined mixed culture showed also bioemulsifying activities in presence of the tested hydrocarbons, which notably helps to solubilize them during the biodegradation process. We could confirm that highest pyrene degradation rate was observed when bioemulsifying activity was maximum in the mixed culture. Our results reveal that defined mixed culture C15 presents sinergistic activity for effective removal of a mix of PAHs, improving the biodegradation process in a valuable approach.
Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Martínez, Fabiana Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
BACTERIAL MIXED CULTURE
PSEUDOMONAS
ACTINOBACTERIA
PAHs REMOVAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/117972
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/117972
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, ArgentinaIsaac, PaulaMartínez, Fabiana LilianBourguignon, NataliaSanchez, Leandro ArturoFerrero, Marcela AlejandraBACTERIAL MIXED CULTUREPSEUDOMONASACTINOBACTERIAPAHs REMOVALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Defined mixed cultures consisting of Pseudomonas and actinobacteria strains showed interesting polycyclic aromatic hydrocarbons (PAHs) removal values, improving greatly removal efficiency of strains in pure culture. These bacterial mixed cultures were synthesized with bacteria isolated from contaminated sites, previously evaluated regarding of naphthalene, phenantrene and pyrene removal when operating at flask scale. The results had shown that monocultures of Pseudomonas monteilii P26 and Pseudomonas sp. N3 could degrade efficiently low molecular weight (LMW) PAHs but they did not show interesting high molecular weight (HMW) PAHs removal capabilities. On the other hand, the actinobacteria Rhodococcus sp. P18, Gordonia sp. H19 and Rhodococcus sp. F27 were able to degrade relatively efficiently HMW PAHs but they did not remove LMW PAHs from culture medium. The combination of four of these five strains (called C15 mixed culture) removed 100 % of naphthalene and phenanthrene, and showed the highest pyrene biodegradation activity with removal values close to 42 %, almost 6-times higher than those values recorded with the strains in pure culture. In addition, this defined mixed culture showed also bioemulsifying activities in presence of the tested hydrocarbons, which notably helps to solubilize them during the biodegradation process. We could confirm that highest pyrene degradation rate was observed when bioemulsifying activity was maximum in the mixed culture. Our results reveal that defined mixed culture C15 presents sinergistic activity for effective removal of a mix of PAHs, improving the biodegradation process in a valuable approach.Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Martínez, Fabiana Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/117972Isaac, Paula; Martínez, Fabiana Lilian; Bourguignon, Natalia; Sanchez, Leandro Arturo; Ferrero, Marcela Alejandra; Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina; Elsevier; International Biodeterioration and Biodegradation; 101; 7-2015; 23-310964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830515000888#info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.03.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:26Zoai:ri.conicet.gov.ar:11336/117972instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:27.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
title Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
spellingShingle Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
Isaac, Paula
BACTERIAL MIXED CULTURE
PSEUDOMONAS
ACTINOBACTERIA
PAHs REMOVAL
title_short Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
title_full Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
title_fullStr Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
title_full_unstemmed Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
title_sort Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina
dc.creator.none.fl_str_mv Isaac, Paula
Martínez, Fabiana Lilian
Bourguignon, Natalia
Sanchez, Leandro Arturo
Ferrero, Marcela Alejandra
author Isaac, Paula
author_facet Isaac, Paula
Martínez, Fabiana Lilian
Bourguignon, Natalia
Sanchez, Leandro Arturo
Ferrero, Marcela Alejandra
author_role author
author2 Martínez, Fabiana Lilian
Bourguignon, Natalia
Sanchez, Leandro Arturo
Ferrero, Marcela Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv BACTERIAL MIXED CULTURE
PSEUDOMONAS
ACTINOBACTERIA
PAHs REMOVAL
topic BACTERIAL MIXED CULTURE
PSEUDOMONAS
ACTINOBACTERIA
PAHs REMOVAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Defined mixed cultures consisting of Pseudomonas and actinobacteria strains showed interesting polycyclic aromatic hydrocarbons (PAHs) removal values, improving greatly removal efficiency of strains in pure culture. These bacterial mixed cultures were synthesized with bacteria isolated from contaminated sites, previously evaluated regarding of naphthalene, phenantrene and pyrene removal when operating at flask scale. The results had shown that monocultures of Pseudomonas monteilii P26 and Pseudomonas sp. N3 could degrade efficiently low molecular weight (LMW) PAHs but they did not show interesting high molecular weight (HMW) PAHs removal capabilities. On the other hand, the actinobacteria Rhodococcus sp. P18, Gordonia sp. H19 and Rhodococcus sp. F27 were able to degrade relatively efficiently HMW PAHs but they did not remove LMW PAHs from culture medium. The combination of four of these five strains (called C15 mixed culture) removed 100 % of naphthalene and phenanthrene, and showed the highest pyrene biodegradation activity with removal values close to 42 %, almost 6-times higher than those values recorded with the strains in pure culture. In addition, this defined mixed culture showed also bioemulsifying activities in presence of the tested hydrocarbons, which notably helps to solubilize them during the biodegradation process. We could confirm that highest pyrene degradation rate was observed when bioemulsifying activity was maximum in the mixed culture. Our results reveal that defined mixed culture C15 presents sinergistic activity for effective removal of a mix of PAHs, improving the biodegradation process in a valuable approach.
Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Martínez, Fabiana Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Defined mixed cultures consisting of Pseudomonas and actinobacteria strains showed interesting polycyclic aromatic hydrocarbons (PAHs) removal values, improving greatly removal efficiency of strains in pure culture. These bacterial mixed cultures were synthesized with bacteria isolated from contaminated sites, previously evaluated regarding of naphthalene, phenantrene and pyrene removal when operating at flask scale. The results had shown that monocultures of Pseudomonas monteilii P26 and Pseudomonas sp. N3 could degrade efficiently low molecular weight (LMW) PAHs but they did not show interesting high molecular weight (HMW) PAHs removal capabilities. On the other hand, the actinobacteria Rhodococcus sp. P18, Gordonia sp. H19 and Rhodococcus sp. F27 were able to degrade relatively efficiently HMW PAHs but they did not remove LMW PAHs from culture medium. The combination of four of these five strains (called C15 mixed culture) removed 100 % of naphthalene and phenanthrene, and showed the highest pyrene biodegradation activity with removal values close to 42 %, almost 6-times higher than those values recorded with the strains in pure culture. In addition, this defined mixed culture showed also bioemulsifying activities in presence of the tested hydrocarbons, which notably helps to solubilize them during the biodegradation process. We could confirm that highest pyrene degradation rate was observed when bioemulsifying activity was maximum in the mixed culture. Our results reveal that defined mixed culture C15 presents sinergistic activity for effective removal of a mix of PAHs, improving the biodegradation process in a valuable approach.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/117972
Isaac, Paula; Martínez, Fabiana Lilian; Bourguignon, Natalia; Sanchez, Leandro Arturo; Ferrero, Marcela Alejandra; Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina; Elsevier; International Biodeterioration and Biodegradation; 101; 7-2015; 23-31
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/117972
identifier_str_mv Isaac, Paula; Martínez, Fabiana Lilian; Bourguignon, Natalia; Sanchez, Leandro Arturo; Ferrero, Marcela Alejandra; Improved PAHs removal performance by a defined bacterial consortium of indigenous Pseudomonas and actinobacteria from Patagonia, Argentina; Elsevier; International Biodeterioration and Biodegradation; 101; 7-2015; 23-31
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830515000888#
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.03.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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