Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium

Autores
Isaac, Paula; Alessandrello, Mauricio; Macedo, Alexandre José; Estévez, María Cristina; Ferrero, Marcela Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, a biofilm-mediated removal of polyaromatic hydrocarbons (PAHs) was proposed. PAHs are toxic and persistent contaminants from petroleum industry, dispersed on water, soils, air and sediments. The removal capabilities of C15 mixed culture of four indigenous strains of Pseudomonas and actinobacteria were successfully corroborated in a previous work. Results showed an interesting removal behavior of planktonic C15 consortium, with degradation capabilities notably enhanced when comparing with pure cultures. In the work reported in this paper, biofilm formation was evaluated in pure cultures and C15 defined consortium in order to propose a biofilm mediated immobilization strategy for bioremediation. Interestingly, a notable enhancement (approx. 180%) in biofilm formation was observed after mixing the four species of C15 compared with pure cultures. Phenanthrene and pyrene also stimulated biofilm arrangement and biofilm-immobilized microorganisms demonstrated a hydrocarbon removal significantly higher than planktonic cells. Maximal removal values were 100% for phenanthrene and 78% for pyrene, after 7 days incubation. The biodegradation assay was divided into two stages: biofilm formation and hydrocarbon removal. The importance of PAHs presence during biofilm formation was evaluated. Although no differences in biofilm structure were observed, bioemulsifier production was enhanced and removal was accelerated when phenanthrene and pyrene were added during biofilm formation stage. Even though more experiments must be conducted, considering the coexistence of Pseudomonas and actinobacteria groups during biodegradation and the improvement of removal performance, interspecies beneficial interactions could be suspected in this native multi-species biofilm.
Fil: Isaac, Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Alessandrello, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Macedo, Alexandre José. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Estévez, María Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
BIODEGRADATION
BIOFILM
MIXED CULTURE
PAHS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99086
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99086
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortiumIsaac, PaulaAlessandrello, MauricioMacedo, Alexandre JoséEstévez, María CristinaFerrero, Marcela AlejandraBIODEGRADATIONBIOFILMMIXED CULTUREPAHShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this work, a biofilm-mediated removal of polyaromatic hydrocarbons (PAHs) was proposed. PAHs are toxic and persistent contaminants from petroleum industry, dispersed on water, soils, air and sediments. The removal capabilities of C15 mixed culture of four indigenous strains of Pseudomonas and actinobacteria were successfully corroborated in a previous work. Results showed an interesting removal behavior of planktonic C15 consortium, with degradation capabilities notably enhanced when comparing with pure cultures. In the work reported in this paper, biofilm formation was evaluated in pure cultures and C15 defined consortium in order to propose a biofilm mediated immobilization strategy for bioremediation. Interestingly, a notable enhancement (approx. 180%) in biofilm formation was observed after mixing the four species of C15 compared with pure cultures. Phenanthrene and pyrene also stimulated biofilm arrangement and biofilm-immobilized microorganisms demonstrated a hydrocarbon removal significantly higher than planktonic cells. Maximal removal values were 100% for phenanthrene and 78% for pyrene, after 7 days incubation. The biodegradation assay was divided into two stages: biofilm formation and hydrocarbon removal. The importance of PAHs presence during biofilm formation was evaluated. Although no differences in biofilm structure were observed, bioemulsifier production was enhanced and removal was accelerated when phenanthrene and pyrene were added during biofilm formation stage. Even though more experiments must be conducted, considering the coexistence of Pseudomonas and actinobacteria groups during biodegradation and the improvement of removal performance, interspecies beneficial interactions could be suspected in this native multi-species biofilm.Fil: Isaac, Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Alessandrello, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Macedo, Alexandre José. Universidade Federal do Rio Grande do Sul; BrasilFil: Estévez, María Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaElsevier2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99086Isaac, Paula; Alessandrello, Mauricio; Macedo, Alexandre José; Estévez, María Cristina; Ferrero, Marcela Alejandra; Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium; Elsevier; Journal of Environmental Chemical Engineering; 5; 2; 4-2017; 1372-13782213-3437CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jece.2017.02.031info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2213343717300830info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:26Zoai:ri.conicet.gov.ar:11336/99086instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:26.342CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
title Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
spellingShingle Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
Isaac, Paula
BIODEGRADATION
BIOFILM
MIXED CULTURE
PAHS
title_short Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
title_full Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
title_fullStr Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
title_full_unstemmed Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
title_sort Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium
dc.creator.none.fl_str_mv Isaac, Paula
Alessandrello, Mauricio
Macedo, Alexandre José
Estévez, María Cristina
Ferrero, Marcela Alejandra
author Isaac, Paula
author_facet Isaac, Paula
Alessandrello, Mauricio
Macedo, Alexandre José
Estévez, María Cristina
Ferrero, Marcela Alejandra
author_role author
author2 Alessandrello, Mauricio
Macedo, Alexandre José
Estévez, María Cristina
Ferrero, Marcela Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIODEGRADATION
BIOFILM
MIXED CULTURE
PAHS
topic BIODEGRADATION
BIOFILM
MIXED CULTURE
PAHS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, a biofilm-mediated removal of polyaromatic hydrocarbons (PAHs) was proposed. PAHs are toxic and persistent contaminants from petroleum industry, dispersed on water, soils, air and sediments. The removal capabilities of C15 mixed culture of four indigenous strains of Pseudomonas and actinobacteria were successfully corroborated in a previous work. Results showed an interesting removal behavior of planktonic C15 consortium, with degradation capabilities notably enhanced when comparing with pure cultures. In the work reported in this paper, biofilm formation was evaluated in pure cultures and C15 defined consortium in order to propose a biofilm mediated immobilization strategy for bioremediation. Interestingly, a notable enhancement (approx. 180%) in biofilm formation was observed after mixing the four species of C15 compared with pure cultures. Phenanthrene and pyrene also stimulated biofilm arrangement and biofilm-immobilized microorganisms demonstrated a hydrocarbon removal significantly higher than planktonic cells. Maximal removal values were 100% for phenanthrene and 78% for pyrene, after 7 days incubation. The biodegradation assay was divided into two stages: biofilm formation and hydrocarbon removal. The importance of PAHs presence during biofilm formation was evaluated. Although no differences in biofilm structure were observed, bioemulsifier production was enhanced and removal was accelerated when phenanthrene and pyrene were added during biofilm formation stage. Even though more experiments must be conducted, considering the coexistence of Pseudomonas and actinobacteria groups during biodegradation and the improvement of removal performance, interspecies beneficial interactions could be suspected in this native multi-species biofilm.
Fil: Isaac, Paula. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Alessandrello, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Macedo, Alexandre José. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Estévez, María Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description In this work, a biofilm-mediated removal of polyaromatic hydrocarbons (PAHs) was proposed. PAHs are toxic and persistent contaminants from petroleum industry, dispersed on water, soils, air and sediments. The removal capabilities of C15 mixed culture of four indigenous strains of Pseudomonas and actinobacteria were successfully corroborated in a previous work. Results showed an interesting removal behavior of planktonic C15 consortium, with degradation capabilities notably enhanced when comparing with pure cultures. In the work reported in this paper, biofilm formation was evaluated in pure cultures and C15 defined consortium in order to propose a biofilm mediated immobilization strategy for bioremediation. Interestingly, a notable enhancement (approx. 180%) in biofilm formation was observed after mixing the four species of C15 compared with pure cultures. Phenanthrene and pyrene also stimulated biofilm arrangement and biofilm-immobilized microorganisms demonstrated a hydrocarbon removal significantly higher than planktonic cells. Maximal removal values were 100% for phenanthrene and 78% for pyrene, after 7 days incubation. The biodegradation assay was divided into two stages: biofilm formation and hydrocarbon removal. The importance of PAHs presence during biofilm formation was evaluated. Although no differences in biofilm structure were observed, bioemulsifier production was enhanced and removal was accelerated when phenanthrene and pyrene were added during biofilm formation stage. Even though more experiments must be conducted, considering the coexistence of Pseudomonas and actinobacteria groups during biodegradation and the improvement of removal performance, interspecies beneficial interactions could be suspected in this native multi-species biofilm.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99086
Isaac, Paula; Alessandrello, Mauricio; Macedo, Alexandre José; Estévez, María Cristina; Ferrero, Marcela Alejandra; Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium; Elsevier; Journal of Environmental Chemical Engineering; 5; 2; 4-2017; 1372-1378
2213-3437
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99086
identifier_str_mv Isaac, Paula; Alessandrello, Mauricio; Macedo, Alexandre José; Estévez, María Cristina; Ferrero, Marcela Alejandra; Pre-exposition to polycyclic aromatic hydrocarbons (PAHs) enhance biofilm formation and hydrocarbon removal by native multi-species consortium; Elsevier; Journal of Environmental Chemical Engineering; 5; 2; 4-2017; 1372-1378
2213-3437
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jece.2017.02.031
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2213343717300830
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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