Bioaugmentation of a biomixture with actinobacteria for atrazine removal

Autores
Ocante, Teresa Ana Lia; Bigliardo, Ana Lucia; Sineli, Pedro Eugenio; Sáez, Juliana María; Benimeli, Claudia Susana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Biopurification systems (BPS) are simple and economical constructions designed to retain and degrade pesticides, in order to reduce or avoid point-source contamination. An organic biomixture (BM) constitutes the most abundant and important component of a BPS. The bioaugmentation of the BM with pesticide-degrading microorganisms is a very interesting approach to optimize its efficiency. Atrazine (ATZ) is a selective herbicide commonly applied to control the appearance of weeds in crops, mainly corn, sorghum, and sugarcane. The aim of this study were to select actinobacteria capable of removing ATZ and to evaluate the effect of the bioaugmentation of a BM with the selected actinobacteria on ATZ dissipation.A qualitative determination of tolerance to ATZ was performed by streaking 14 actinobacteria strains on Petri dishes containing starch casein agar medium with a central channel containing the ATZ solution (1000 mg L-1 and 50000 mg L-1). The tolerant strains were used to perform a quantitative determination of their ability to grow in the presence of ATZ (50 mg L-1) and to remove it from liquid minimal medium (MM). Controls were carried out in MM supplemented with glucose (1 g L-1). Microbial growth (dry weight) and residual concentration of ATZ (gas chromatography, GC) were determined after 96 h. The actinobacterium which presented the highest removal of ATZ and did not show growth inhibition in the presence of ATZ was used to inoculate a BM composed of soil, straw, and agricultural sugarcane crop residue (25:25:50). Periodic samples were collected to determine residual ATZ, total heterotrophic microorganisms (CFU g-1 BM), fluorescein diacetate hydrolysis (FDA) and acid phosphatase (AP) activities.All actinobacteria were highly tolerant to 1000 mg L-1 of ATZ and moderately tolerant to 50000 mg L-1 of ATZ. All of them were able to grow in MM supplemented with ATZ, however most of them reached statistically lower biomass than the obtained with glucose as carbon source, with the exception of Streptomyces sp. A2, A11, and M7. These strains were able to use ATZ as carbon source and to remove it from MM, presenting removal values ranging between 10% and 75%. Based on the microbial growth and ATZ removal, Streptomyces sp. M7 was selected to bioaugment the BM. The inoculation of the BM with Streptomyces sp. M7 improved significantly the ATZ removal (58%) respect to the non-biougmented BM (38%) after 28 days of incubation. The total heterotrophic population in the bioaugmented and contaminated BM (4.55 x 107 CFU g-1) did not present statistical variation respect to non-inoculated control, nor non-contaminated control. Enzymatic activities obtained in these systems ranged between 44.9 ± 0.03 and 87.2 ± 20.3 g fluorescein g-1 h-1 for FDA, and 91.82 ± 5.7 and 159.0 ± 9.0 µg p-nitrophenol g-1 h-1 for AP. These results suggest that the bioaugmentation of BM with Streptomyces sp. M7 represents a promising tool to reduce ATZ concentration in BPS.
Fil: Ocante, Teresa Ana Lia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bigliardo, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina
Fil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
BIOAUGMENTATION
BIOMIXTURE
ATRAZINE
ACTINOBACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/177690

id CONICETDig_8e07dbda1c3d9620781e1e3a3b8484b2
oai_identifier_str oai:ri.conicet.gov.ar:11336/177690
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bioaugmentation of a biomixture with actinobacteria for atrazine removalOcante, Teresa Ana LiaBigliardo, Ana LuciaSineli, Pedro EugenioSáez, Juliana MaríaBenimeli, Claudia SusanaBIOAUGMENTATIONBIOMIXTUREATRAZINEACTINOBACTERIAhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Biopurification systems (BPS) are simple and economical constructions designed to retain and degrade pesticides, in order to reduce or avoid point-source contamination. An organic biomixture (BM) constitutes the most abundant and important component of a BPS. The bioaugmentation of the BM with pesticide-degrading microorganisms is a very interesting approach to optimize its efficiency. Atrazine (ATZ) is a selective herbicide commonly applied to control the appearance of weeds in crops, mainly corn, sorghum, and sugarcane. The aim of this study were to select actinobacteria capable of removing ATZ and to evaluate the effect of the bioaugmentation of a BM with the selected actinobacteria on ATZ dissipation.A qualitative determination of tolerance to ATZ was performed by streaking 14 actinobacteria strains on Petri dishes containing starch casein agar medium with a central channel containing the ATZ solution (1000 mg L-1 and 50000 mg L-1). The tolerant strains were used to perform a quantitative determination of their ability to grow in the presence of ATZ (50 mg L-1) and to remove it from liquid minimal medium (MM). Controls were carried out in MM supplemented with glucose (1 g L-1). Microbial growth (dry weight) and residual concentration of ATZ (gas chromatography, GC) were determined after 96 h. The actinobacterium which presented the highest removal of ATZ and did not show growth inhibition in the presence of ATZ was used to inoculate a BM composed of soil, straw, and agricultural sugarcane crop residue (25:25:50). Periodic samples were collected to determine residual ATZ, total heterotrophic microorganisms (CFU g-1 BM), fluorescein diacetate hydrolysis (FDA) and acid phosphatase (AP) activities.All actinobacteria were highly tolerant to 1000 mg L-1 of ATZ and moderately tolerant to 50000 mg L-1 of ATZ. All of them were able to grow in MM supplemented with ATZ, however most of them reached statistically lower biomass than the obtained with glucose as carbon source, with the exception of Streptomyces sp. A2, A11, and M7. These strains were able to use ATZ as carbon source and to remove it from MM, presenting removal values ranging between 10% and 75%. Based on the microbial growth and ATZ removal, Streptomyces sp. M7 was selected to bioaugment the BM. The inoculation of the BM with Streptomyces sp. M7 improved significantly the ATZ removal (58%) respect to the non-biougmented BM (38%) after 28 days of incubation. The total heterotrophic population in the bioaugmented and contaminated BM (4.55 x 107 CFU g-1) did not present statistical variation respect to non-inoculated control, nor non-contaminated control. Enzymatic activities obtained in these systems ranged between 44.9 ± 0.03 and 87.2 ± 20.3 g fluorescein g-1 h-1 for FDA, and 91.82 ± 5.7 and 159.0 ± 9.0 µg p-nitrophenol g-1 h-1 for AP. These results suggest that the bioaugmentation of BM with Streptomyces sp. M7 represents a promising tool to reduce ATZ concentration in BPS.Fil: Ocante, Teresa Ana Lia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Bigliardo, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Sineli, Pedro Eugenio. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; ArgentinaFil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressSaltaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/177690Bioaugmentation of a biomixture with actinobacteria for atrazine removal; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-91667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:18Zoai:ri.conicet.gov.ar:11336/177690instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:19.194CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bioaugmentation of a biomixture with actinobacteria for atrazine removal
title Bioaugmentation of a biomixture with actinobacteria for atrazine removal
spellingShingle Bioaugmentation of a biomixture with actinobacteria for atrazine removal
Ocante, Teresa Ana Lia
BIOAUGMENTATION
BIOMIXTURE
ATRAZINE
ACTINOBACTERIA
title_short Bioaugmentation of a biomixture with actinobacteria for atrazine removal
title_full Bioaugmentation of a biomixture with actinobacteria for atrazine removal
title_fullStr Bioaugmentation of a biomixture with actinobacteria for atrazine removal
title_full_unstemmed Bioaugmentation of a biomixture with actinobacteria for atrazine removal
title_sort Bioaugmentation of a biomixture with actinobacteria for atrazine removal
dc.creator.none.fl_str_mv Ocante, Teresa Ana Lia
Bigliardo, Ana Lucia
Sineli, Pedro Eugenio
Sáez, Juliana María
Benimeli, Claudia Susana
author Ocante, Teresa Ana Lia
author_facet Ocante, Teresa Ana Lia
Bigliardo, Ana Lucia
Sineli, Pedro Eugenio
Sáez, Juliana María
Benimeli, Claudia Susana
author_role author
author2 Bigliardo, Ana Lucia
Sineli, Pedro Eugenio
Sáez, Juliana María
Benimeli, Claudia Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOAUGMENTATION
BIOMIXTURE
ATRAZINE
ACTINOBACTERIA
topic BIOAUGMENTATION
BIOMIXTURE
ATRAZINE
ACTINOBACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Biopurification systems (BPS) are simple and economical constructions designed to retain and degrade pesticides, in order to reduce or avoid point-source contamination. An organic biomixture (BM) constitutes the most abundant and important component of a BPS. The bioaugmentation of the BM with pesticide-degrading microorganisms is a very interesting approach to optimize its efficiency. Atrazine (ATZ) is a selective herbicide commonly applied to control the appearance of weeds in crops, mainly corn, sorghum, and sugarcane. The aim of this study were to select actinobacteria capable of removing ATZ and to evaluate the effect of the bioaugmentation of a BM with the selected actinobacteria on ATZ dissipation.A qualitative determination of tolerance to ATZ was performed by streaking 14 actinobacteria strains on Petri dishes containing starch casein agar medium with a central channel containing the ATZ solution (1000 mg L-1 and 50000 mg L-1). The tolerant strains were used to perform a quantitative determination of their ability to grow in the presence of ATZ (50 mg L-1) and to remove it from liquid minimal medium (MM). Controls were carried out in MM supplemented with glucose (1 g L-1). Microbial growth (dry weight) and residual concentration of ATZ (gas chromatography, GC) were determined after 96 h. The actinobacterium which presented the highest removal of ATZ and did not show growth inhibition in the presence of ATZ was used to inoculate a BM composed of soil, straw, and agricultural sugarcane crop residue (25:25:50). Periodic samples were collected to determine residual ATZ, total heterotrophic microorganisms (CFU g-1 BM), fluorescein diacetate hydrolysis (FDA) and acid phosphatase (AP) activities.All actinobacteria were highly tolerant to 1000 mg L-1 of ATZ and moderately tolerant to 50000 mg L-1 of ATZ. All of them were able to grow in MM supplemented with ATZ, however most of them reached statistically lower biomass than the obtained with glucose as carbon source, with the exception of Streptomyces sp. A2, A11, and M7. These strains were able to use ATZ as carbon source and to remove it from MM, presenting removal values ranging between 10% and 75%. Based on the microbial growth and ATZ removal, Streptomyces sp. M7 was selected to bioaugment the BM. The inoculation of the BM with Streptomyces sp. M7 improved significantly the ATZ removal (58%) respect to the non-biougmented BM (38%) after 28 days of incubation. The total heterotrophic population in the bioaugmented and contaminated BM (4.55 x 107 CFU g-1) did not present statistical variation respect to non-inoculated control, nor non-contaminated control. Enzymatic activities obtained in these systems ranged between 44.9 ± 0.03 and 87.2 ± 20.3 g fluorescein g-1 h-1 for FDA, and 91.82 ± 5.7 and 159.0 ± 9.0 µg p-nitrophenol g-1 h-1 for AP. These results suggest that the bioaugmentation of BM with Streptomyces sp. M7 represents a promising tool to reduce ATZ concentration in BPS.
Fil: Ocante, Teresa Ana Lia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Bigliardo, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica; Argentina
Fil: Sáez, Juliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Biopurification systems (BPS) are simple and economical constructions designed to retain and degrade pesticides, in order to reduce or avoid point-source contamination. An organic biomixture (BM) constitutes the most abundant and important component of a BPS. The bioaugmentation of the BM with pesticide-degrading microorganisms is a very interesting approach to optimize its efficiency. Atrazine (ATZ) is a selective herbicide commonly applied to control the appearance of weeds in crops, mainly corn, sorghum, and sugarcane. The aim of this study were to select actinobacteria capable of removing ATZ and to evaluate the effect of the bioaugmentation of a BM with the selected actinobacteria on ATZ dissipation.A qualitative determination of tolerance to ATZ was performed by streaking 14 actinobacteria strains on Petri dishes containing starch casein agar medium with a central channel containing the ATZ solution (1000 mg L-1 and 50000 mg L-1). The tolerant strains were used to perform a quantitative determination of their ability to grow in the presence of ATZ (50 mg L-1) and to remove it from liquid minimal medium (MM). Controls were carried out in MM supplemented with glucose (1 g L-1). Microbial growth (dry weight) and residual concentration of ATZ (gas chromatography, GC) were determined after 96 h. The actinobacterium which presented the highest removal of ATZ and did not show growth inhibition in the presence of ATZ was used to inoculate a BM composed of soil, straw, and agricultural sugarcane crop residue (25:25:50). Periodic samples were collected to determine residual ATZ, total heterotrophic microorganisms (CFU g-1 BM), fluorescein diacetate hydrolysis (FDA) and acid phosphatase (AP) activities.All actinobacteria were highly tolerant to 1000 mg L-1 of ATZ and moderately tolerant to 50000 mg L-1 of ATZ. All of them were able to grow in MM supplemented with ATZ, however most of them reached statistically lower biomass than the obtained with glucose as carbon source, with the exception of Streptomyces sp. A2, A11, and M7. These strains were able to use ATZ as carbon source and to remove it from MM, presenting removal values ranging between 10% and 75%. Based on the microbial growth and ATZ removal, Streptomyces sp. M7 was selected to bioaugment the BM. The inoculation of the BM with Streptomyces sp. M7 improved significantly the ATZ removal (58%) respect to the non-biougmented BM (38%) after 28 days of incubation. The total heterotrophic population in the bioaugmented and contaminated BM (4.55 x 107 CFU g-1) did not present statistical variation respect to non-inoculated control, nor non-contaminated control. Enzymatic activities obtained in these systems ranged between 44.9 ± 0.03 and 87.2 ± 20.3 g fluorescein g-1 h-1 for FDA, and 91.82 ± 5.7 and 159.0 ± 9.0 µg p-nitrophenol g-1 h-1 for AP. These results suggest that the bioaugmentation of BM with Streptomyces sp. M7 represents a promising tool to reduce ATZ concentration in BPS.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/177690
Bioaugmentation of a biomixture with actinobacteria for atrazine removal; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-9
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/177690
identifier_str_mv Bioaugmentation of a biomixture with actinobacteria for atrazine removal; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-9
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019-version-final.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269023855706112
score 13.13397