Furfural removal from liquid systems by actinobacteria

Autores
Echeverría, M. C.; Benimeli, Claudia Susana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Many industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g l−1. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg l-1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12, A14 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg l-1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg l-1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p < 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.
Fil: Echeverría, M. C.. Universidad Tecnológica Nacional. Facultad Regional Resistencia; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
XVII Congreso Argentino de Microbiología General
Los Cocos
Argentina
Sociedad Argentina de Microbiología General
Materia
FURFURAL
ACTINOBACTERIA
CONSORTIUM
BIOREMEDIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201378

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spelling Furfural removal from liquid systems by actinobacteriaEcheverría, M. C.Benimeli, Claudia SusanaFURFURALACTINOBACTERIACONSORTIUMBIOREMEDIATIONhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Many industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g l−1. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg l-1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12, A14 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg l-1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg l-1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p < 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.Fil: Echeverría, M. C.. Universidad Tecnológica Nacional. Facultad Regional Resistencia; ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; ArgentinaXVII Congreso Argentino de Microbiología GeneralLos CocosArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201378Furfural removal from liquid systems by actinobacteria; XVII Congreso Argentino de Microbiología General; Los Cocos; Argentina; 2022; 70-71CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-de-Resumenes-SAMIGE-2022_final.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:39Zoai:ri.conicet.gov.ar:11336/201378instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:40.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Furfural removal from liquid systems by actinobacteria
title Furfural removal from liquid systems by actinobacteria
spellingShingle Furfural removal from liquid systems by actinobacteria
Echeverría, M. C.
FURFURAL
ACTINOBACTERIA
CONSORTIUM
BIOREMEDIATION
title_short Furfural removal from liquid systems by actinobacteria
title_full Furfural removal from liquid systems by actinobacteria
title_fullStr Furfural removal from liquid systems by actinobacteria
title_full_unstemmed Furfural removal from liquid systems by actinobacteria
title_sort Furfural removal from liquid systems by actinobacteria
dc.creator.none.fl_str_mv Echeverría, M. C.
Benimeli, Claudia Susana
author Echeverría, M. C.
author_facet Echeverría, M. C.
Benimeli, Claudia Susana
author_role author
author2 Benimeli, Claudia Susana
author2_role author
dc.subject.none.fl_str_mv FURFURAL
ACTINOBACTERIA
CONSORTIUM
BIOREMEDIATION
topic FURFURAL
ACTINOBACTERIA
CONSORTIUM
BIOREMEDIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Many industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g l−1. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg l-1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12, A14 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg l-1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg l-1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p < 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.
Fil: Echeverría, M. C.. Universidad Tecnológica Nacional. Facultad Regional Resistencia; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales; Argentina
XVII Congreso Argentino de Microbiología General
Los Cocos
Argentina
Sociedad Argentina de Microbiología General
description Many industries such as petrochemical, pulp and paper, pharmaceutical, and food industries involve processes that use or produce furfural. Furfural is a heterocyclic aldehyde obtained by dehydrating at high temperatures of xylose; therefore, it is a characteristic compound present in acid hydrolyzates in which the furfural concentration can usually reach 2–3 g l−1. In the region Northeast of Argentina (NEA), furfural is produced from detanized quebracho sawdust. In NEA, wastewaters derived from furfural production contain around 800 mg l-1 of this compound, which can cause toxic effects on living systems if they are released into the environment without proper treatment. In the present work, the removal of different concentrations of furfural by actinobacteria from liquid systems was studied. Isolates of actinobacteria called L4, L6, L9 and L13 obtained from sediments of stabilization ponds of a furfural-producing plant in the NEA region, and Streptomyces sp. A5, A6, A12, A14 and M7, obtained from sites contaminated with other xenobiotic compounds, were selected on base of their tolerance to furfural in Starch Casein Agar medium. In order to select the most efficient actinobacteria with respect to their growth and furfural removal ability in liquid medium, Minimal Medium (MM) added with a furfural concentration of 418±1 mg l-1 as the only carbon and energy source was used. This selection was carried out by determining the minimum relationship between the concentration of residual furfural and the microbial growth. Streptomyces sp. A12 and M7 and strain L9 were selected because they showed the minimal relationship. Subsequently, the selected strains, as pure and mixed cultures, were inoculated in MM supplemented with furfural 807±10 mg l-1 as the only carbon and energy source. The results showed that the three pure cultures were able to grow and develop under these conditions; however, the culture for which the relationship mentioned above was minimal, was the consortium formed by the actinobacteria L9, A12 and M7. In order to evaluate the effectiveness of the bioremediation process, ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). The culture supernatants were evaluated before and after its treatment for each condition. In response, inhibition of germination and elongation of the radicle and hypocotyl were determined in the presence of furfural. Significant increases in these bioindicators (p < 0.05) were obtained when the treatment was carried out with the consortium formed by the actinobacteria L9, A12 and M7. The results obtained suggest that the selected actinobacteria consortium represents a promising bioremediation tool for the treatment of effluents containing furfural.
publishDate 2022
dc.date.none.fl_str_mv 2022
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Book
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201378
Furfural removal from liquid systems by actinobacteria; XVII Congreso Argentino de Microbiología General; Los Cocos; Argentina; 2022; 70-71
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201378
identifier_str_mv Furfural removal from liquid systems by actinobacteria; XVII Congreso Argentino de Microbiología General; Los Cocos; Argentina; 2022; 70-71
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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