Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating

Autores
Goutman, Juan Diego; Escobar, Ariel Luis Manuel; Calvo, Daniel Juan
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
1 Lanthanide-induced modulation of GABA(C) receptors expressed in Xenopus oocytes was studied. 2 We obtained two-electrode voltage-clamp recordings of ionic currents mediated by recombinant homomeric GABA ρ1 receptors and performed numerical simulations of kinetic models of the macroscopic ionic currents. 3 GABA-evoked chloride currents were potentiated by La 3+, Lu 3+ and Gd 3+ in the micromolar range. 4 Lanthanide effects were rapid, reversible and voltage independent. 5 The degree of potentiation was reduced by increasing GABA concentration. 6 Lu 3+ also induced receptor desensitization and decreased the deactivation rate of GABA ρ1 currents. 7 In the presence of 300 μM Lu 3+, dose-response curves for GABA-evoked currents showed a significant enhancement of the maximum amplitude and an increase of the apparent affinity. 8 The rate of onset of TPMPA and picrotoxin antagonism of GABA ρ1 receptors was modulated by Lu 3+. 9 These results suggest that the potentiation of the anionic current was the result of a direct lanthanide-receptor interaction at a site capable of allosterically modulating channel properties. 10 Based on kinetic schemes, which included a second open state and a nonconducting desensitized state that closely reproduced the experimental results, two nonexclusive probable models of GABA ρ1 channels gating are proposed.
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Escobar, Ariel Luis Manuel. Texas Tech University Health Sciences Center; Estados Unidos
Fil: Calvo, Daniel Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Materia
Cationic Modulation
Chloride Channels
Gaba
Gaba C Receptors
Human Retina
Kinetic Modeling
Lanthanides
Xenopus Oocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79868
Acceder
id CONICETDig_d468d99c8154945b3af77422d4278822
oai_identifier_str oai:ri.conicet.gov.ar:11336/79868
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gatingGoutman, Juan DiegoEscobar, Ariel Luis ManuelCalvo, Daniel JuanCationic ModulationChloride ChannelsGabaGaba C ReceptorsHuman RetinaKinetic ModelingLanthanidesXenopus Oocyteshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/31 Lanthanide-induced modulation of GABA(C) receptors expressed in Xenopus oocytes was studied. 2 We obtained two-electrode voltage-clamp recordings of ionic currents mediated by recombinant homomeric GABA ρ1 receptors and performed numerical simulations of kinetic models of the macroscopic ionic currents. 3 GABA-evoked chloride currents were potentiated by La 3+, Lu 3+ and Gd 3+ in the micromolar range. 4 Lanthanide effects were rapid, reversible and voltage independent. 5 The degree of potentiation was reduced by increasing GABA concentration. 6 Lu 3+ also induced receptor desensitization and decreased the deactivation rate of GABA ρ1 currents. 7 In the presence of 300 μM Lu 3+, dose-response curves for GABA-evoked currents showed a significant enhancement of the maximum amplitude and an increase of the apparent affinity. 8 The rate of onset of TPMPA and picrotoxin antagonism of GABA ρ1 receptors was modulated by Lu 3+. 9 These results suggest that the potentiation of the anionic current was the result of a direct lanthanide-receptor interaction at a site capable of allosterically modulating channel properties. 10 Based on kinetic schemes, which included a second open state and a nonconducting desensitized state that closely reproduced the experimental results, two nonexclusive probable models of GABA ρ1 channels gating are proposed.Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Escobar, Ariel Luis Manuel. Texas Tech University Health Sciences Center; Estados UnidosFil: Calvo, Daniel Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaWiley Blackwell Publishing, Inc2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79868Goutman, Juan Diego; Escobar, Ariel Luis Manuel; Calvo, Daniel Juan; Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating; Wiley Blackwell Publishing, Inc; British Journal of Pharmacology; 146; 7; 12-2005; 1000-10090007-1188CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1751227/info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.bjp.0706411info:eu-repo/semantics/altIdentifier/url/https://bpspubs.onlinelibrary.wiley.com/doi/full/10.1038/sj.bjp.0706411info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:43:25Zoai:ri.conicet.gov.ar:11336/79868instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:43:25.237CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
title Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
spellingShingle Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
Goutman, Juan Diego
Cationic Modulation
Chloride Channels
Gaba
Gaba C Receptors
Human Retina
Kinetic Modeling
Lanthanides
Xenopus Oocytes
title_short Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
title_full Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
title_fullStr Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
title_full_unstemmed Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
title_sort Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating
dc.creator.none.fl_str_mv Goutman, Juan Diego
Escobar, Ariel Luis Manuel
Calvo, Daniel Juan
author Goutman, Juan Diego
author_facet Goutman, Juan Diego
Escobar, Ariel Luis Manuel
Calvo, Daniel Juan
author_role author
author2 Escobar, Ariel Luis Manuel
Calvo, Daniel Juan
author2_role author
author
dc.subject.none.fl_str_mv Cationic Modulation
Chloride Channels
Gaba
Gaba C Receptors
Human Retina
Kinetic Modeling
Lanthanides
Xenopus Oocytes
topic Cationic Modulation
Chloride Channels
Gaba
Gaba C Receptors
Human Retina
Kinetic Modeling
Lanthanides
Xenopus Oocytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv 1 Lanthanide-induced modulation of GABA(C) receptors expressed in Xenopus oocytes was studied. 2 We obtained two-electrode voltage-clamp recordings of ionic currents mediated by recombinant homomeric GABA ρ1 receptors and performed numerical simulations of kinetic models of the macroscopic ionic currents. 3 GABA-evoked chloride currents were potentiated by La 3+, Lu 3+ and Gd 3+ in the micromolar range. 4 Lanthanide effects were rapid, reversible and voltage independent. 5 The degree of potentiation was reduced by increasing GABA concentration. 6 Lu 3+ also induced receptor desensitization and decreased the deactivation rate of GABA ρ1 currents. 7 In the presence of 300 μM Lu 3+, dose-response curves for GABA-evoked currents showed a significant enhancement of the maximum amplitude and an increase of the apparent affinity. 8 The rate of onset of TPMPA and picrotoxin antagonism of GABA ρ1 receptors was modulated by Lu 3+. 9 These results suggest that the potentiation of the anionic current was the result of a direct lanthanide-receptor interaction at a site capable of allosterically modulating channel properties. 10 Based on kinetic schemes, which included a second open state and a nonconducting desensitized state that closely reproduced the experimental results, two nonexclusive probable models of GABA ρ1 channels gating are proposed.
Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Escobar, Ariel Luis Manuel. Texas Tech University Health Sciences Center; Estados Unidos
Fil: Calvo, Daniel Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
description 1 Lanthanide-induced modulation of GABA(C) receptors expressed in Xenopus oocytes was studied. 2 We obtained two-electrode voltage-clamp recordings of ionic currents mediated by recombinant homomeric GABA ρ1 receptors and performed numerical simulations of kinetic models of the macroscopic ionic currents. 3 GABA-evoked chloride currents were potentiated by La 3+, Lu 3+ and Gd 3+ in the micromolar range. 4 Lanthanide effects were rapid, reversible and voltage independent. 5 The degree of potentiation was reduced by increasing GABA concentration. 6 Lu 3+ also induced receptor desensitization and decreased the deactivation rate of GABA ρ1 currents. 7 In the presence of 300 μM Lu 3+, dose-response curves for GABA-evoked currents showed a significant enhancement of the maximum amplitude and an increase of the apparent affinity. 8 The rate of onset of TPMPA and picrotoxin antagonism of GABA ρ1 receptors was modulated by Lu 3+. 9 These results suggest that the potentiation of the anionic current was the result of a direct lanthanide-receptor interaction at a site capable of allosterically modulating channel properties. 10 Based on kinetic schemes, which included a second open state and a nonconducting desensitized state that closely reproduced the experimental results, two nonexclusive probable models of GABA ρ1 channels gating are proposed.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79868
Goutman, Juan Diego; Escobar, Ariel Luis Manuel; Calvo, Daniel Juan; Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating; Wiley Blackwell Publishing, Inc; British Journal of Pharmacology; 146; 7; 12-2005; 1000-1009
0007-1188
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79868
identifier_str_mv Goutman, Juan Diego; Escobar, Ariel Luis Manuel; Calvo, Daniel Juan; Analysis of macroscopic ionic currents mediated by GABA ρ1 receptors during lanthanide modulation predicts novel states controlling channel gating; Wiley Blackwell Publishing, Inc; British Journal of Pharmacology; 146; 7; 12-2005; 1000-1009
0007-1188
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1751227/
info:eu-repo/semantics/altIdentifier/doi/10.1038/sj.bjp.0706411
info:eu-repo/semantics/altIdentifier/url/https://bpspubs.onlinelibrary.wiley.com/doi/full/10.1038/sj.bjp.0706411
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847427461020647424
score 13.10058

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