Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics

Autores
Suzuki, Masahiko; Tada, Asuka; Kanmani, Paulraj; Watanabe, Hitoshi; Aso, Hisashi; Suda, Yoshihito; Nochi, Tomonori; Miyazawa, Kenji; Yoda, Kazutoyo; He, Fang; Hosoda, Masataka; Saito, Tadao; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functionalmature adipocytes fromPIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer?s patches (PPs) immune-competent cells.We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20,Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered.
Fil: Suzuki, Masahiko . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Tada, Asuka . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Kanmani, Paulraj . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Watanabe, Hitoshi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito . Miyagi University. Department of Food, Agriculture and Environment; Japón
Fil: Nochi, Tomonori . Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Miyazawa, Kenji . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: Yoda, Kazutoyo . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: He, Fang . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: Hosoda, Masataka . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: Saito, Tadao . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Kitazawa, Haruki . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Materia
PORCINE INTRAMUSCULAR ADIPOCYTES
IMMUNOBIOTICS
INFLAMMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7742
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobioticsSuzuki, Masahiko Tada, Asuka Kanmani, Paulraj Watanabe, HitoshiAso, HisashiSuda, Yoshihito Nochi, Tomonori Miyazawa, Kenji Yoda, Kazutoyo He, Fang Hosoda, Masataka Saito, Tadao Villena, Julio CesarKitazawa, Haruki PORCINE INTRAMUSCULAR ADIPOCYTESIMMUNOBIOTICSINFLAMMATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functionalmature adipocytes fromPIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer?s patches (PPs) immune-competent cells.We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20,Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered.Fil: Suzuki, Masahiko . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; JapónFil: Tada, Asuka . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; JapónFil: Kanmani, Paulraj . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; JapónFil: Watanabe, Hitoshi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; JapónFil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; JapónFil: Suda, Yoshihito . Miyagi University. Department of Food, Agriculture and Environment; JapónFil: Nochi, Tomonori . Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; JapónFil: Miyazawa, Kenji . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; JapónFil: Yoda, Kazutoyo . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; JapónFil: He, Fang . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; JapónFil: Hosoda, Masataka . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; JapónFil: Saito, Tadao . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Kitazawa, Haruki . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; JapónPublic Library Of Science2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7742Suzuki, Masahiko ; Tada, Asuka ; Kanmani, Paulraj ; Watanabe, Hitoshi; Aso, Hisashi; et al.; Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics; Public Library Of Science; Plos One; 10; 3; 3-2015; e01196441932-62031932-6203enginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366390/info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0119644info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119644info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:25:55Zoai:ri.conicet.gov.ar:11336/7742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:25:55.672CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
title Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
spellingShingle Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
Suzuki, Masahiko
PORCINE INTRAMUSCULAR ADIPOCYTES
IMMUNOBIOTICS
INFLAMMATION
title_short Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
title_full Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
title_fullStr Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
title_full_unstemmed Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
title_sort Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics
dc.creator.none.fl_str_mv Suzuki, Masahiko
Tada, Asuka
Kanmani, Paulraj
Watanabe, Hitoshi
Aso, Hisashi
Suda, Yoshihito
Nochi, Tomonori
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Hosoda, Masataka
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author Suzuki, Masahiko
author_facet Suzuki, Masahiko
Tada, Asuka
Kanmani, Paulraj
Watanabe, Hitoshi
Aso, Hisashi
Suda, Yoshihito
Nochi, Tomonori
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Hosoda, Masataka
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Tada, Asuka
Kanmani, Paulraj
Watanabe, Hitoshi
Aso, Hisashi
Suda, Yoshihito
Nochi, Tomonori
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Hosoda, Masataka
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PORCINE INTRAMUSCULAR ADIPOCYTES
IMMUNOBIOTICS
INFLAMMATION
topic PORCINE INTRAMUSCULAR ADIPOCYTES
IMMUNOBIOTICS
INFLAMMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functionalmature adipocytes fromPIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer?s patches (PPs) immune-competent cells.We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20,Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered.
Fil: Suzuki, Masahiko . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Tada, Asuka . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Kanmani, Paulraj . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Watanabe, Hitoshi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito . Miyagi University. Department of Food, Agriculture and Environment; Japón
Fil: Nochi, Tomonori . Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Miyazawa, Kenji . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: Yoda, Kazutoyo . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: He, Fang . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: Hosoda, Masataka . Takanashi Milk Products Co., Ltd. Technical Research Laboratory; Japón
Fil: Saito, Tadao . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Kitazawa, Haruki . Tohoku University. Graduate School of Agricultural Science. Food and Feed Immunology Group; Japón
description We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functionalmature adipocytes fromPIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer?s patches (PPs) immune-competent cells.We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20,Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7742
Suzuki, Masahiko ; Tada, Asuka ; Kanmani, Paulraj ; Watanabe, Hitoshi; Aso, Hisashi; et al.; Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics; Public Library Of Science; Plos One; 10; 3; 3-2015; e0119644
1932-6203
1932-6203
url http://hdl.handle.net/11336/7742
identifier_str_mv Suzuki, Masahiko ; Tada, Asuka ; Kanmani, Paulraj ; Watanabe, Hitoshi; Aso, Hisashi; et al.; Advanced application of porcine intramuscular adipocytes for evaluating anti-adipogenic and anti-inflammatory activities of immunobiotics; Public Library Of Science; Plos One; 10; 3; 3-2015; e0119644
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366390/
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0119644
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119644
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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