Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunob...

Autores
Tada, Asuka; Kober, Akm Humayun; Islam, Md Aminul; Igata, Manami; Takagi, Michihiro; Suzuki, Masahiko; Aso, Hisashi; Ikeda Ohtsubo, Wakako; Yoda, Kazutoyo; Miyazawa, Kenji; He, Fang; Takahashi, Hideki; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The degree of fat accumulation and adipokine production are two major indicators of obesity that are correlated with increased adipose tissue mass and chronic inflammatory responses. Adipocytes have been considered effector cells for the inflammatory responses due to their capacity to express Toll-like receptors (TLRs). In this study, we evaluated the degree of fat accumulation and adipokine production in porcine intramuscular preadipocyte (PIP) cells maintained for in vitro differentiation over a long period without or with stimulation of either TNF-α or TLR2-, TLR3-, or TLR4-ligands. The cytosolic fat accumulation was measured by liquid chromatography and the expression of adipokines (CCL2, IL-6, IL-8 and IL-10) were quantified by RT-qPCR and ELISA at several time points (0 to 20 days) of PIP cells differentiation. Long-term adipogenic differentiation (LTAD) induced a progressive fat accumulation in the adipocytes over time. Activation of TLR3 and TLR4 resulted in an increased rate of fat accumulation into the adipocytes over the LTAD. The production of CCL2, IL-8 and IL-6 were significantly increased in unstimulated adipocytes during the LTAD, while IL-10 expression remained stable over the studied period. An increasing trend of adiponectin and leptin production was also observed during the LTAD. On the other hand, the stimulation of adipocytes with TLRs agonists or TNF-α resulted in an increasing trend of CCL2, IL-6 and IL-8 production while IL-10 remained stable in all four treatments during the LTAD. We also examined the influences of several immunoregulatory probiotic strains (immunobiotics) on the modulation of the fat accumulation and adipokine production using supernatants of immunobiotic-treated intestinal immune cells and the LTAD of PIP cells. Immunobiotics have shown a strain-specific ability to modulate the fat accumulation and adipokine production, and differentiation of adipocytes. Here, we expanded the utility and potential application of our in vitro PIP cells model by evaluating an LTAD period (20 days) in order to elucidate further insights of chronic inflammatory pathobiology of adipocytes associated with obesity as well as to explore the prospects of immunomodulatory intervention for obesity such as immunobiotics.
Fil: Tada, Asuka. Tohoku University; Japón
Fil: Kober, Akm Humayun. Chittagong Veterinary and Animal Sciences University; Bangladesh. Tohoku University; Japón
Fil: Islam, Md Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; Japón
Fil: Igata, Manami. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Suzuki, Masahiko. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Yoda, Kazutoyo. Takanashi Milk Products Co., Ltd.; Japón
Fil: Miyazawa, Kenji. Takanashi Milk Products Co., Ltd.; Japón
Fil: He, Fang. Takanashi Milk Products Co., Ltd.; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
ADIPOKINES
FAT ACCUMULATION
IMMUNOBIOTICS
INFLAMMATION
LONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD)
PORCINE INTRAMUSCULAR ADIPOCYTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/124709
Acceder
id CONICETDig_c9d6822c935987ae7fd4fed610057322
oai_identifier_str oai:ri.conicet.gov.ar:11336/124709
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobioticsTada, AsukaKober, Akm HumayunIslam, Md AminulIgata, ManamiTakagi, MichihiroSuzuki, MasahikoAso, HisashiIkeda Ohtsubo, WakakoYoda, KazutoyoMiyazawa, KenjiHe, FangTakahashi, HidekiVillena, Julio CesarKitazawa, HarukiADIPOKINESFAT ACCUMULATIONIMMUNOBIOTICSINFLAMMATIONLONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD)PORCINE INTRAMUSCULAR ADIPOCYTEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The degree of fat accumulation and adipokine production are two major indicators of obesity that are correlated with increased adipose tissue mass and chronic inflammatory responses. Adipocytes have been considered effector cells for the inflammatory responses due to their capacity to express Toll-like receptors (TLRs). In this study, we evaluated the degree of fat accumulation and adipokine production in porcine intramuscular preadipocyte (PIP) cells maintained for in vitro differentiation over a long period without or with stimulation of either TNF-α or TLR2-, TLR3-, or TLR4-ligands. The cytosolic fat accumulation was measured by liquid chromatography and the expression of adipokines (CCL2, IL-6, IL-8 and IL-10) were quantified by RT-qPCR and ELISA at several time points (0 to 20 days) of PIP cells differentiation. Long-term adipogenic differentiation (LTAD) induced a progressive fat accumulation in the adipocytes over time. Activation of TLR3 and TLR4 resulted in an increased rate of fat accumulation into the adipocytes over the LTAD. The production of CCL2, IL-8 and IL-6 were significantly increased in unstimulated adipocytes during the LTAD, while IL-10 expression remained stable over the studied period. An increasing trend of adiponectin and leptin production was also observed during the LTAD. On the other hand, the stimulation of adipocytes with TLRs agonists or TNF-α resulted in an increasing trend of CCL2, IL-6 and IL-8 production while IL-10 remained stable in all four treatments during the LTAD. We also examined the influences of several immunoregulatory probiotic strains (immunobiotics) on the modulation of the fat accumulation and adipokine production using supernatants of immunobiotic-treated intestinal immune cells and the LTAD of PIP cells. Immunobiotics have shown a strain-specific ability to modulate the fat accumulation and adipokine production, and differentiation of adipocytes. Here, we expanded the utility and potential application of our in vitro PIP cells model by evaluating an LTAD period (20 days) in order to elucidate further insights of chronic inflammatory pathobiology of adipocytes associated with obesity as well as to explore the prospects of immunomodulatory intervention for obesity such as immunobiotics.Fil: Tada, Asuka. Tohoku University; JapónFil: Kober, Akm Humayun. Chittagong Veterinary and Animal Sciences University; Bangladesh. Tohoku University; JapónFil: Islam, Md Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; JapónFil: Igata, Manami. Tohoku University; JapónFil: Takagi, Michihiro. Tohoku University; JapónFil: Suzuki, Masahiko. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Yoda, Kazutoyo. Takanashi Milk Products Co., Ltd.; JapónFil: Miyazawa, Kenji. Takanashi Milk Products Co., Ltd.; JapónFil: He, Fang. Takanashi Milk Products Co., Ltd.; JapónFil: Takahashi, Hideki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónMolecular Diversity Preservation International2020-07-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124709Tada, Asuka; Kober, Akm Humayun; Islam, Md Aminul; Igata, Manami; Takagi, Michihiro; et al.; Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics; Molecular Diversity Preservation International; Cells; 9; 7; 17-7-2020; 1-22;17152073-44092073-4409CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/cells9071715info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4409/9/7/1715info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:43:28Zoai:ri.conicet.gov.ar:11336/124709instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:43:29.257CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
title Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
spellingShingle Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
Tada, Asuka
ADIPOKINES
FAT ACCUMULATION
IMMUNOBIOTICS
INFLAMMATION
LONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD)
PORCINE INTRAMUSCULAR ADIPOCYTE
title_short Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
title_full Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
title_fullStr Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
title_full_unstemmed Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
title_sort Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics
dc.creator.none.fl_str_mv Tada, Asuka
Kober, Akm Humayun
Islam, Md Aminul
Igata, Manami
Takagi, Michihiro
Suzuki, Masahiko
Aso, Hisashi
Ikeda Ohtsubo, Wakako
Yoda, Kazutoyo
Miyazawa, Kenji
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Kitazawa, Haruki
author Tada, Asuka
author_facet Tada, Asuka
Kober, Akm Humayun
Islam, Md Aminul
Igata, Manami
Takagi, Michihiro
Suzuki, Masahiko
Aso, Hisashi
Ikeda Ohtsubo, Wakako
Yoda, Kazutoyo
Miyazawa, Kenji
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Kober, Akm Humayun
Islam, Md Aminul
Igata, Manami
Takagi, Michihiro
Suzuki, Masahiko
Aso, Hisashi
Ikeda Ohtsubo, Wakako
Yoda, Kazutoyo
Miyazawa, Kenji
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADIPOKINES
FAT ACCUMULATION
IMMUNOBIOTICS
INFLAMMATION
LONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD)
PORCINE INTRAMUSCULAR ADIPOCYTE
topic ADIPOKINES
FAT ACCUMULATION
IMMUNOBIOTICS
INFLAMMATION
LONG-TERM ADIPOGENIC DIFFERENTIATION (LTAD)
PORCINE INTRAMUSCULAR ADIPOCYTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The degree of fat accumulation and adipokine production are two major indicators of obesity that are correlated with increased adipose tissue mass and chronic inflammatory responses. Adipocytes have been considered effector cells for the inflammatory responses due to their capacity to express Toll-like receptors (TLRs). In this study, we evaluated the degree of fat accumulation and adipokine production in porcine intramuscular preadipocyte (PIP) cells maintained for in vitro differentiation over a long period without or with stimulation of either TNF-α or TLR2-, TLR3-, or TLR4-ligands. The cytosolic fat accumulation was measured by liquid chromatography and the expression of adipokines (CCL2, IL-6, IL-8 and IL-10) were quantified by RT-qPCR and ELISA at several time points (0 to 20 days) of PIP cells differentiation. Long-term adipogenic differentiation (LTAD) induced a progressive fat accumulation in the adipocytes over time. Activation of TLR3 and TLR4 resulted in an increased rate of fat accumulation into the adipocytes over the LTAD. The production of CCL2, IL-8 and IL-6 were significantly increased in unstimulated adipocytes during the LTAD, while IL-10 expression remained stable over the studied period. An increasing trend of adiponectin and leptin production was also observed during the LTAD. On the other hand, the stimulation of adipocytes with TLRs agonists or TNF-α resulted in an increasing trend of CCL2, IL-6 and IL-8 production while IL-10 remained stable in all four treatments during the LTAD. We also examined the influences of several immunoregulatory probiotic strains (immunobiotics) on the modulation of the fat accumulation and adipokine production using supernatants of immunobiotic-treated intestinal immune cells and the LTAD of PIP cells. Immunobiotics have shown a strain-specific ability to modulate the fat accumulation and adipokine production, and differentiation of adipocytes. Here, we expanded the utility and potential application of our in vitro PIP cells model by evaluating an LTAD period (20 days) in order to elucidate further insights of chronic inflammatory pathobiology of adipocytes associated with obesity as well as to explore the prospects of immunomodulatory intervention for obesity such as immunobiotics.
Fil: Tada, Asuka. Tohoku University; Japón
Fil: Kober, Akm Humayun. Chittagong Veterinary and Animal Sciences University; Bangladesh. Tohoku University; Japón
Fil: Islam, Md Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; Japón
Fil: Igata, Manami. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Suzuki, Masahiko. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Yoda, Kazutoyo. Takanashi Milk Products Co., Ltd.; Japón
Fil: Miyazawa, Kenji. Takanashi Milk Products Co., Ltd.; Japón
Fil: He, Fang. Takanashi Milk Products Co., Ltd.; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description The degree of fat accumulation and adipokine production are two major indicators of obesity that are correlated with increased adipose tissue mass and chronic inflammatory responses. Adipocytes have been considered effector cells for the inflammatory responses due to their capacity to express Toll-like receptors (TLRs). In this study, we evaluated the degree of fat accumulation and adipokine production in porcine intramuscular preadipocyte (PIP) cells maintained for in vitro differentiation over a long period without or with stimulation of either TNF-α or TLR2-, TLR3-, or TLR4-ligands. The cytosolic fat accumulation was measured by liquid chromatography and the expression of adipokines (CCL2, IL-6, IL-8 and IL-10) were quantified by RT-qPCR and ELISA at several time points (0 to 20 days) of PIP cells differentiation. Long-term adipogenic differentiation (LTAD) induced a progressive fat accumulation in the adipocytes over time. Activation of TLR3 and TLR4 resulted in an increased rate of fat accumulation into the adipocytes over the LTAD. The production of CCL2, IL-8 and IL-6 were significantly increased in unstimulated adipocytes during the LTAD, while IL-10 expression remained stable over the studied period. An increasing trend of adiponectin and leptin production was also observed during the LTAD. On the other hand, the stimulation of adipocytes with TLRs agonists or TNF-α resulted in an increasing trend of CCL2, IL-6 and IL-8 production while IL-10 remained stable in all four treatments during the LTAD. We also examined the influences of several immunoregulatory probiotic strains (immunobiotics) on the modulation of the fat accumulation and adipokine production using supernatants of immunobiotic-treated intestinal immune cells and the LTAD of PIP cells. Immunobiotics have shown a strain-specific ability to modulate the fat accumulation and adipokine production, and differentiation of adipocytes. Here, we expanded the utility and potential application of our in vitro PIP cells model by evaluating an LTAD period (20 days) in order to elucidate further insights of chronic inflammatory pathobiology of adipocytes associated with obesity as well as to explore the prospects of immunomodulatory intervention for obesity such as immunobiotics.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/124709
Tada, Asuka; Kober, Akm Humayun; Islam, Md Aminul; Igata, Manami; Takagi, Michihiro; et al.; Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics; Molecular Diversity Preservation International; Cells; 9; 7; 17-7-2020; 1-22;1715
2073-4409
2073-4409
CONICET Digital
CONICET
url http://hdl.handle.net/11336/124709
identifier_str_mv Tada, Asuka; Kober, Akm Humayun; Islam, Md Aminul; Igata, Manami; Takagi, Michihiro; et al.; Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics; Molecular Diversity Preservation International; Cells; 9; 7; 17-7-2020; 1-22;1715
2073-4409
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/cells9071715
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4409/9/7/1715
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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