Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin

Autores
Tada, Asuka; Islam, Md Aminul; Kober, Akm Humayun; Fukuyama, Kohtaro; Takagi, Michihiro; Igata, Manami; Albarracín, Leonardo Miguel; Ikeda Ohtsubo, Wakako; Miyazawa, Kenji; Yoda, Kazutoyo; He, Fang; Takahashi, Hideki; Villena, Julio Cesar; Aso, Hisashi; Kitazawa, Haruki
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the ‘PPAR signaling’ and ‘insulin resistance’ pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of ′GPCR ligand-binding′, ‘cell chemotaxis’, ‘blood coagulation and complement’, ‘metabolism of lipid and lipoproteins’, ‘regulation of lipid metabolism by PPARA’, and ‘lipid digestion, mobilization and transport’ pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.
Fil: Tada, Asuka. Tohoku University; Japón
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Kober, Akm Humayun. Tohoku University; Japón
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Igata, Manami. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Miyazawa, Kenji. Takanashi Milk Products Co.; Japón
Fil: Yoda, Kazutoyo. Takanashi Milk Products Co.; Japón
Fil: He, Fang. Takanashi Milk Products Co.; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
ADIPOGENESIS
PORCINE INTRAMUSCULAR PREADIPOCYTE (PIP)
PORCINE MATURE ADIPOCYTE (PMA)
SEROTONIN
SEROTONIN RECEPTOR
TNF-Α
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/127234
Acceder
id CONICETDig_a33171fa4724108b598c4aaaab521a0c
oai_identifier_str oai:ri.conicet.gov.ar:11336/127234
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and SerotoninTada, AsukaIslam, Md AminulKober, Akm HumayunFukuyama, KohtaroTakagi, MichihiroIgata, ManamiAlbarracín, Leonardo MiguelIkeda Ohtsubo, WakakoMiyazawa, KenjiYoda, KazutoyoHe, FangTakahashi, HidekiVillena, Julio CesarAso, HisashiKitazawa, HarukiADIPOGENESISPORCINE INTRAMUSCULAR PREADIPOCYTE (PIP)PORCINE MATURE ADIPOCYTE (PMA)SEROTONINSEROTONIN RECEPTORTNF-ΑTRANSCRIPTOMEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the ‘PPAR signaling’ and ‘insulin resistance’ pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of ′GPCR ligand-binding′, ‘cell chemotaxis’, ‘blood coagulation and complement’, ‘metabolism of lipid and lipoproteins’, ‘regulation of lipid metabolism by PPARA’, and ‘lipid digestion, mobilization and transport’ pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.Fil: Tada, Asuka. Tohoku University; JapónFil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; BangladeshFil: Kober, Akm Humayun. Tohoku University; JapónFil: Fukuyama, Kohtaro. Tohoku University; JapónFil: Takagi, Michihiro. Tohoku University; JapónFil: Igata, Manami. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Miyazawa, Kenji. Takanashi Milk Products Co.; JapónFil: Yoda, Kazutoyo. Takanashi Milk Products Co.; JapónFil: He, Fang. Takanashi Milk Products Co.; JapónFil: Takahashi, Hideki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónMolecular Diversity Preservation International2020-01-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/127234Tada, Asuka; Islam, Md Aminul; Kober, Akm Humayun; Fukuyama, Kohtaro; Takagi, Michihiro; et al.; Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 21; 2; 18-1-2020; 1-20; 6381661-65961422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/ijms21020638info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/21/2/638info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:21Zoai:ri.conicet.gov.ar:11336/127234instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:21.681CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
title Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
spellingShingle Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
Tada, Asuka
ADIPOGENESIS
PORCINE INTRAMUSCULAR PREADIPOCYTE (PIP)
PORCINE MATURE ADIPOCYTE (PMA)
SEROTONIN
SEROTONIN RECEPTOR
TNF-Α
TRANSCRIPTOME
title_short Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
title_full Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
title_fullStr Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
title_full_unstemmed Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
title_sort Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin
dc.creator.none.fl_str_mv Tada, Asuka
Islam, Md Aminul
Kober, Akm Humayun
Fukuyama, Kohtaro
Takagi, Michihiro
Igata, Manami
Albarracín, Leonardo Miguel
Ikeda Ohtsubo, Wakako
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Aso, Hisashi
Kitazawa, Haruki
author Tada, Asuka
author_facet Tada, Asuka
Islam, Md Aminul
Kober, Akm Humayun
Fukuyama, Kohtaro
Takagi, Michihiro
Igata, Manami
Albarracín, Leonardo Miguel
Ikeda Ohtsubo, Wakako
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Aso, Hisashi
Kitazawa, Haruki
author_role author
author2 Islam, Md Aminul
Kober, Akm Humayun
Fukuyama, Kohtaro
Takagi, Michihiro
Igata, Manami
Albarracín, Leonardo Miguel
Ikeda Ohtsubo, Wakako
Miyazawa, Kenji
Yoda, Kazutoyo
He, Fang
Takahashi, Hideki
Villena, Julio Cesar
Aso, Hisashi
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADIPOGENESIS
PORCINE INTRAMUSCULAR PREADIPOCYTE (PIP)
PORCINE MATURE ADIPOCYTE (PMA)
SEROTONIN
SEROTONIN RECEPTOR
TNF-Α
TRANSCRIPTOME
topic ADIPOGENESIS
PORCINE INTRAMUSCULAR PREADIPOCYTE (PIP)
PORCINE MATURE ADIPOCYTE (PMA)
SEROTONIN
SEROTONIN RECEPTOR
TNF-Α
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the ‘PPAR signaling’ and ‘insulin resistance’ pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of ′GPCR ligand-binding′, ‘cell chemotaxis’, ‘blood coagulation and complement’, ‘metabolism of lipid and lipoproteins’, ‘regulation of lipid metabolism by PPARA’, and ‘lipid digestion, mobilization and transport’ pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.
Fil: Tada, Asuka. Tohoku University; Japón
Fil: Islam, Md Aminul. Tohoku University; Japón. Bangladesh Agricultural University; Bangladesh
Fil: Kober, Akm Humayun. Tohoku University; Japón
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Igata, Manami. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Miyazawa, Kenji. Takanashi Milk Products Co.; Japón
Fil: Yoda, Kazutoyo. Takanashi Milk Products Co.; Japón
Fil: He, Fang. Takanashi Milk Products Co.; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the ‘PPAR signaling’ and ‘insulin resistance’ pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of ′GPCR ligand-binding′, ‘cell chemotaxis’, ‘blood coagulation and complement’, ‘metabolism of lipid and lipoproteins’, ‘regulation of lipid metabolism by PPARA’, and ‘lipid digestion, mobilization and transport’ pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/127234
Tada, Asuka; Islam, Md Aminul; Kober, Akm Humayun; Fukuyama, Kohtaro; Takagi, Michihiro; et al.; Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 21; 2; 18-1-2020; 1-20; 638
1661-6596
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/127234
identifier_str_mv Tada, Asuka; Islam, Md Aminul; Kober, Akm Humayun; Fukuyama, Kohtaro; Takagi, Michihiro; et al.; Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 21; 2; 18-1-2020; 1-20; 638
1661-6596
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms21020638
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/21/2/638
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980260892639232
score 12.993085

Publicaciones similares