Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies

Autores
Oresti, Gerardo Martin; Reyes, Juan G.; Luquez, Jessica Mariela; Pediconi, Maria Filomena; Aveldaño, Marta Isabel
Año de publicación
2008
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this work pachytene spermatocytes (PS) and round spermatids (RS) were isolated to study how ceramides (Cer) and sphingomyelin (SM) with VLCPUFA are formed in the testis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their polyenoic fatty acids (FA). In glycerophopholipids, the 20:4n-6/22:5n-6 ratio was significantly higher in PS than in RS. In SM (and Cer), 28:4n-6, 30:5n-6, 32:5n-6 were the main FA in PS, whereas 28:4n-6 predominated in RS. By contrast, in the latter, an important proportion of SM (and Cer) FA were recovered as a-hydroxylated versions of VLCPUFA (2-OH 28:4 to 32:5). The residual bodies (RB) contain materials –including lipids– that are discarded from late spermatids (elongated forms) as they differentiate to spermatozoa, to be phagocytized and “recycled” in Sertoli cells. The RB were very rich in glycerophopholipids and triglycerides with 22:5n-6. The SM of RB contained virtually no VLCPUFA but was extremely rich in 2-OH VLCPUFA. The results point to fatty acid desaturase, elongase and hydroxylase activities that are highly specific for long-chain and very long-chain polyenoic fatty acids encoded by genes that are expressed sequentially as cell- differentiation proceeds
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Juan G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pediconi, Maria Filomena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
44th Annual Meeting Argentine Society for Biochemistry and Molecular Biology Research; XLIV Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
SPERMATOGENIC CELLS
CERAMIDES
SPHINGOMYELIN
VERY LONG-CHAIN POLYENOIC FATTY ACIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247013
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/247013
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodiesOresti, Gerardo MartinReyes, Juan G.Luquez, Jessica MarielaPediconi, Maria FilomenaAveldaño, Marta IsabelSPERMATOGENIC CELLSCERAMIDESSPHINGOMYELINVERY LONG-CHAIN POLYENOIC FATTY ACIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this work pachytene spermatocytes (PS) and round spermatids (RS) were isolated to study how ceramides (Cer) and sphingomyelin (SM) with VLCPUFA are formed in the testis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their polyenoic fatty acids (FA). In glycerophopholipids, the 20:4n-6/22:5n-6 ratio was significantly higher in PS than in RS. In SM (and Cer), 28:4n-6, 30:5n-6, 32:5n-6 were the main FA in PS, whereas 28:4n-6 predominated in RS. By contrast, in the latter, an important proportion of SM (and Cer) FA were recovered as a-hydroxylated versions of VLCPUFA (2-OH 28:4 to 32:5). The residual bodies (RB) contain materials –including lipids– that are discarded from late spermatids (elongated forms) as they differentiate to spermatozoa, to be phagocytized and “recycled” in Sertoli cells. The RB were very rich in glycerophopholipids and triglycerides with 22:5n-6. The SM of RB contained virtually no VLCPUFA but was extremely rich in 2-OH VLCPUFA. The results point to fatty acid desaturase, elongase and hydroxylase activities that are highly specific for long-chain and very long-chain polyenoic fatty acids encoded by genes that are expressed sequentially as cell- differentiation proceedsFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Reyes, Juan G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Pontificia Universidad Católica de Valparaíso; ChileFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pediconi, Maria Filomena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina44th Annual Meeting Argentine Society for Biochemistry and Molecular Biology Research; XLIV Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularVilla Carlos PazArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología “Dr. Mario H. Burgos”2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247013Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies; 44th Annual Meeting Argentine Society for Biochemistry and Molecular Biology Research; XLIV Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Villa Carlos Paz; Argentina; 2008; 92-920327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-32.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:56:59Zoai:ri.conicet.gov.ar:11336/247013instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:56:59.379CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
title Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
spellingShingle Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
Oresti, Gerardo Martin
SPERMATOGENIC CELLS
CERAMIDES
SPHINGOMYELIN
VERY LONG-CHAIN POLYENOIC FATTY ACIDS
title_short Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
title_full Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
title_fullStr Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
title_full_unstemmed Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
title_sort Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Reyes, Juan G.
Luquez, Jessica Mariela
Pediconi, Maria Filomena
Aveldaño, Marta Isabel
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Reyes, Juan G.
Luquez, Jessica Mariela
Pediconi, Maria Filomena
Aveldaño, Marta Isabel
author_role author
author2 Reyes, Juan G.
Luquez, Jessica Mariela
Pediconi, Maria Filomena
Aveldaño, Marta Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv SPERMATOGENIC CELLS
CERAMIDES
SPHINGOMYELIN
VERY LONG-CHAIN POLYENOIC FATTY ACIDS
topic SPERMATOGENIC CELLS
CERAMIDES
SPHINGOMYELIN
VERY LONG-CHAIN POLYENOIC FATTY ACIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this work pachytene spermatocytes (PS) and round spermatids (RS) were isolated to study how ceramides (Cer) and sphingomyelin (SM) with VLCPUFA are formed in the testis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their polyenoic fatty acids (FA). In glycerophopholipids, the 20:4n-6/22:5n-6 ratio was significantly higher in PS than in RS. In SM (and Cer), 28:4n-6, 30:5n-6, 32:5n-6 were the main FA in PS, whereas 28:4n-6 predominated in RS. By contrast, in the latter, an important proportion of SM (and Cer) FA were recovered as a-hydroxylated versions of VLCPUFA (2-OH 28:4 to 32:5). The residual bodies (RB) contain materials –including lipids– that are discarded from late spermatids (elongated forms) as they differentiate to spermatozoa, to be phagocytized and “recycled” in Sertoli cells. The RB were very rich in glycerophopholipids and triglycerides with 22:5n-6. The SM of RB contained virtually no VLCPUFA but was extremely rich in 2-OH VLCPUFA. The results point to fatty acid desaturase, elongase and hydroxylase activities that are highly specific for long-chain and very long-chain polyenoic fatty acids encoded by genes that are expressed sequentially as cell- differentiation proceeds
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Juan G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pediconi, Maria Filomena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
44th Annual Meeting Argentine Society for Biochemistry and Molecular Biology Research; XLIV Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this work pachytene spermatocytes (PS) and round spermatids (RS) were isolated to study how ceramides (Cer) and sphingomyelin (SM) with VLCPUFA are formed in the testis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their polyenoic fatty acids (FA). In glycerophopholipids, the 20:4n-6/22:5n-6 ratio was significantly higher in PS than in RS. In SM (and Cer), 28:4n-6, 30:5n-6, 32:5n-6 were the main FA in PS, whereas 28:4n-6 predominated in RS. By contrast, in the latter, an important proportion of SM (and Cer) FA were recovered as a-hydroxylated versions of VLCPUFA (2-OH 28:4 to 32:5). The residual bodies (RB) contain materials –including lipids– that are discarded from late spermatids (elongated forms) as they differentiate to spermatozoa, to be phagocytized and “recycled” in Sertoli cells. The RB were very rich in glycerophopholipids and triglycerides with 22:5n-6. The SM of RB contained virtually no VLCPUFA but was extremely rich in 2-OH VLCPUFA. The results point to fatty acid desaturase, elongase and hydroxylase activities that are highly specific for long-chain and very long-chain polyenoic fatty acids encoded by genes that are expressed sequentially as cell- differentiation proceeds
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247013
Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies; 44th Annual Meeting Argentine Society for Biochemistry and Molecular Biology Research; XLIV Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Villa Carlos Paz; Argentina; 2008; 92-92
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247013
identifier_str_mv Lipids with very long chain fatty acids (VLCPUFA) in germ cells and residual bodies; 44th Annual Meeting Argentine Society for Biochemistry and Molecular Biology Research; XLIV Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Villa Carlos Paz; Argentina; 2008; 92-92
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-32.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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