Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation

Autores
Oresti, Gerardo Martin; Reyes, J. G.; Aveldaño, Marta Isabel
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this study, pachytene spermatocytes and round spermatids were isolated from rat seminiferous tubules to study how ceramides (Cer) and sphingomyelins (SM) with very long chain polyunsaturated fatty acids (VLCPUFA) change during spermatogenesis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their fatty acids (FA). SM and Cer had 28:4n-6, 30:5n6 and 32:5n-6 as their main FA in spermatocytes, but both lipids contained mostly 28:4n-6 in spermatids. In turn, spermatids, but not spermatocytes, contained an important proportion of 2-hydroxylated versions of the mentioned VLCPUFA (2-OH VLCPUFA). The 2-OH VLCPUFA / non-hydroxy VLCPUFA ratio not only increased markedly in SM and Cer with germ cell differentiation but was even higher in (epididymal) spermatozoa. Although the enzyme that is responsible for the synthesis of the 2-OH VLCPUFA of SM and Cer remains to be investigated, our results suggest that it is expressed after completion of meiosis. Residual bodies, which contain materials -including lipids- that are discarded from late spermatids (elongatingcondensing forms) as they differentiate to spermatozoa, were unusual in that they contained no Cer but plenty of SM with almost exclusively 2-OH VLCPUFA. Taking into account that residual bodies are normally phagocytosed by Sertoli cells, the eventual fate of these SM species is another intriguing question to be solved regarding sphingolipid metabolism during the spermatogenic cycle
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, J. G.. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Eukaryotic Lipids: Treasure of Regulatory Information - Workshop Federation of European Biochemical Societies
Spetses
Grecia
Federation of European Biochemical Societies
Materia
differentiation
spermatogenic cells
sphingomyelins, ceramides
very long chain polyunsaturated fatty acids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232683
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/232683
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiationOresti, Gerardo MartinReyes, J. G.Aveldaño, Marta Isabeldifferentiationspermatogenic cellssphingomyelins, ceramidesvery long chain polyunsaturated fatty acidshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this study, pachytene spermatocytes and round spermatids were isolated from rat seminiferous tubules to study how ceramides (Cer) and sphingomyelins (SM) with very long chain polyunsaturated fatty acids (VLCPUFA) change during spermatogenesis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their fatty acids (FA). SM and Cer had 28:4n-6, 30:5n6 and 32:5n-6 as their main FA in spermatocytes, but both lipids contained mostly 28:4n-6 in spermatids. In turn, spermatids, but not spermatocytes, contained an important proportion of 2-hydroxylated versions of the mentioned VLCPUFA (2-OH VLCPUFA). The 2-OH VLCPUFA / non-hydroxy VLCPUFA ratio not only increased markedly in SM and Cer with germ cell differentiation but was even higher in (epididymal) spermatozoa. Although the enzyme that is responsible for the synthesis of the 2-OH VLCPUFA of SM and Cer remains to be investigated, our results suggest that it is expressed after completion of meiosis. Residual bodies, which contain materials -including lipids- that are discarded from late spermatids (elongatingcondensing forms) as they differentiate to spermatozoa, were unusual in that they contained no Cer but plenty of SM with almost exclusively 2-OH VLCPUFA. Taking into account that residual bodies are normally phagocytosed by Sertoli cells, the eventual fate of these SM species is another intriguing question to be solved regarding sphingolipid metabolism during the spermatogenic cycleFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Reyes, J. G.. Pontificia Universidad Católica de Valparaíso; ChileFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaEukaryotic Lipids: Treasure of Regulatory Information - Workshop Federation of European Biochemical SocietiesSpetsesGreciaFederation of European Biochemical SocietiesFederation of European Biochemical Societies2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectWorkshopBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232683Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation; Eukaryotic Lipids: Treasure of Regulatory Information - Workshop Federation of European Biochemical Societies; Spetses; Grecia; 2010; 45-45CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://web.science.uu.nl/archive/Spetses2010/posters.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:55:43Zoai:ri.conicet.gov.ar:11336/232683instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:55:44.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
title Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
spellingShingle Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
Oresti, Gerardo Martin
differentiation
spermatogenic cells
sphingomyelins, ceramides
very long chain polyunsaturated fatty acids
title_short Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
title_full Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
title_fullStr Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
title_full_unstemmed Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
title_sort Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Reyes, J. G.
Aveldaño, Marta Isabel
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Reyes, J. G.
Aveldaño, Marta Isabel
author_role author
author2 Reyes, J. G.
Aveldaño, Marta Isabel
author2_role author
author
dc.subject.none.fl_str_mv differentiation
spermatogenic cells
sphingomyelins, ceramides
very long chain polyunsaturated fatty acids
topic differentiation
spermatogenic cells
sphingomyelins, ceramides
very long chain polyunsaturated fatty acids
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this study, pachytene spermatocytes and round spermatids were isolated from rat seminiferous tubules to study how ceramides (Cer) and sphingomyelins (SM) with very long chain polyunsaturated fatty acids (VLCPUFA) change during spermatogenesis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their fatty acids (FA). SM and Cer had 28:4n-6, 30:5n6 and 32:5n-6 as their main FA in spermatocytes, but both lipids contained mostly 28:4n-6 in spermatids. In turn, spermatids, but not spermatocytes, contained an important proportion of 2-hydroxylated versions of the mentioned VLCPUFA (2-OH VLCPUFA). The 2-OH VLCPUFA / non-hydroxy VLCPUFA ratio not only increased markedly in SM and Cer with germ cell differentiation but was even higher in (epididymal) spermatozoa. Although the enzyme that is responsible for the synthesis of the 2-OH VLCPUFA of SM and Cer remains to be investigated, our results suggest that it is expressed after completion of meiosis. Residual bodies, which contain materials -including lipids- that are discarded from late spermatids (elongatingcondensing forms) as they differentiate to spermatozoa, were unusual in that they contained no Cer but plenty of SM with almost exclusively 2-OH VLCPUFA. Taking into account that residual bodies are normally phagocytosed by Sertoli cells, the eventual fate of these SM species is another intriguing question to be solved regarding sphingolipid metabolism during the spermatogenic cycle
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, J. G.. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Eukaryotic Lipids: Treasure of Regulatory Information - Workshop Federation of European Biochemical Societies
Spetses
Grecia
Federation of European Biochemical Societies
description In seminiferous tubules, Sertoli cells and spermatogenic cells at different stages of differentiation coexist. In this study, pachytene spermatocytes and round spermatids were isolated from rat seminiferous tubules to study how ceramides (Cer) and sphingomyelins (SM) with very long chain polyunsaturated fatty acids (VLCPUFA) change during spermatogenesis. Although the concentration of phospholipids including SM was similar in both cell types, there were many differences in their fatty acids (FA). SM and Cer had 28:4n-6, 30:5n6 and 32:5n-6 as their main FA in spermatocytes, but both lipids contained mostly 28:4n-6 in spermatids. In turn, spermatids, but not spermatocytes, contained an important proportion of 2-hydroxylated versions of the mentioned VLCPUFA (2-OH VLCPUFA). The 2-OH VLCPUFA / non-hydroxy VLCPUFA ratio not only increased markedly in SM and Cer with germ cell differentiation but was even higher in (epididymal) spermatozoa. Although the enzyme that is responsible for the synthesis of the 2-OH VLCPUFA of SM and Cer remains to be investigated, our results suggest that it is expressed after completion of meiosis. Residual bodies, which contain materials -including lipids- that are discarded from late spermatids (elongatingcondensing forms) as they differentiate to spermatozoa, were unusual in that they contained no Cer but plenty of SM with almost exclusively 2-OH VLCPUFA. Taking into account that residual bodies are normally phagocytosed by Sertoli cells, the eventual fate of these SM species is another intriguing question to be solved regarding sphingolipid metabolism during the spermatogenic cycle
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Workshop
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232683
Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation; Eukaryotic Lipids: Treasure of Regulatory Information - Workshop Federation of European Biochemical Societies; Spetses; Grecia; 2010; 45-45
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232683
identifier_str_mv Changes in sphingomyelins and ceramides with very long-chain polyunsaturated fatty acids during spermatogenic cell differentiation; Eukaryotic Lipids: Treasure of Regulatory Information - Workshop Federation of European Biochemical Societies; Spetses; Grecia; 2010; 45-45
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://web.science.uu.nl/archive/Spetses2010/posters.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Federation of European Biochemical Societies
publisher.none.fl_str_mv Federation of European Biochemical Societies
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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