Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and g...

Autores
Montel Mendoza, María Gabriela; Ale, Cesar Emmanuel; Nader, Maria Elena Fatima; Pasteris, Sergio Enrique
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enterococcus gallinarum CRL 1826 isolated from an American bullfrog (Lithobates catesbeianus) skin inhibits the growth of Citrobacter freundii, Pseudomonas aeruginosa (bullfrog pathogens) and Listeria monocytogenes by a synergistic effect between organic acids and a bacteriocin-like molecule. This bacteriocin, named enterocin CRL 1826, showed a proteinaceous nature, heat stability and polar characteristics. Its production followed kinetics of primary metabolites synthesis reaching a maximum of 61,400 AU/mL. The minimum inhibitory and minimumbactericidal concentrations were 2,640 and 5,280 AU/mL, respectively, against L. monocytogenes. The addition of 120,000 AU/mL of enterocin to growing L. monocytogenes and Gram-negative (P. aeruginosa and C. freundii) bacteria showed bactericidal and bacteriostatic effects, respectively. However, enterocin derived-peptides had bactericidal effect only against Gram-negatives.Enterocin produced cell envelope damages and efflux of citosolic content on L. monocytogenes, while enterocin derived-peptides showed granulation and contraction of cytoplasm material on P. aeruginosa and increase in theperiplasmic space and empty cells appearance on C. freundii.Enterocin CRL 1826 is the first bacteriocin described for E. gallinarum from raniculture. It could be used as a biopreservative while the derived-peptides represent an alternative to control multi-drug resistant Gram-negatives.The antimicrobial spectrum and the stability of enterocin and its derived-peptides indicate that they could be applied in different biotechnological areas.
Fil: Montel Mendoza, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Ale, Cesar Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Materia
ENTEROCOCCUS GALLINARUM
RANICULTURE
BACTERIOCIN
PROBIOTICS
BIOPRESERVATIVES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28259
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/28259
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negativesMontel Mendoza, María GabrielaAle, Cesar EmmanuelNader, Maria Elena FatimaPasteris, Sergio EnriqueENTEROCOCCUS GALLINARUMRANICULTUREBACTERIOCINPROBIOTICSBIOPRESERVATIVEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Enterococcus gallinarum CRL 1826 isolated from an American bullfrog (Lithobates catesbeianus) skin inhibits the growth of Citrobacter freundii, Pseudomonas aeruginosa (bullfrog pathogens) and Listeria monocytogenes by a synergistic effect between organic acids and a bacteriocin-like molecule. This bacteriocin, named enterocin CRL 1826, showed a proteinaceous nature, heat stability and polar characteristics. Its production followed kinetics of primary metabolites synthesis reaching a maximum of 61,400 AU/mL. The minimum inhibitory and minimumbactericidal concentrations were 2,640 and 5,280 AU/mL, respectively, against L. monocytogenes. The addition of 120,000 AU/mL of enterocin to growing L. monocytogenes and Gram-negative (P. aeruginosa and C. freundii) bacteria showed bactericidal and bacteriostatic effects, respectively. However, enterocin derived-peptides had bactericidal effect only against Gram-negatives.Enterocin produced cell envelope damages and efflux of citosolic content on L. monocytogenes, while enterocin derived-peptides showed granulation and contraction of cytoplasm material on P. aeruginosa and increase in theperiplasmic space and empty cells appearance on C. freundii.Enterocin CRL 1826 is the first bacteriocin described for E. gallinarum from raniculture. It could be used as a biopreservative while the derived-peptides represent an alternative to control multi-drug resistant Gram-negatives.The antimicrobial spectrum and the stability of enterocin and its derived-peptides indicate that they could be applied in different biotechnological areas.Fil: Montel Mendoza, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Ale, Cesar Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaOmics International2015-08-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28259Montel Mendoza, María Gabriela; Ale, Cesar Emmanuel; Nader, Maria Elena Fatima; Pasteris, Sergio Enrique; Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives; Omics International; Journal of Bioprocessing & Biotechniques; 5; 8; 24-8-2015; 1-9;10002502155-9821CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9821.1000250info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/characterization-of-a-bacteriocin-produced-by-enterococcus-gallinarum-crl-1826-isolated-from-captive-bullfrog-evaluation-of-its-mode-of-action-against-listeria-monocytogenes-and-gramnegatives-2155-9821-1000250.php?aid=58843info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:35Zoai:ri.conicet.gov.ar:11336/28259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:35.593CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
title Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
spellingShingle Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
Montel Mendoza, María Gabriela
ENTEROCOCCUS GALLINARUM
RANICULTURE
BACTERIOCIN
PROBIOTICS
BIOPRESERVATIVES
title_short Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
title_full Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
title_fullStr Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
title_full_unstemmed Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
title_sort Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives
dc.creator.none.fl_str_mv Montel Mendoza, María Gabriela
Ale, Cesar Emmanuel
Nader, Maria Elena Fatima
Pasteris, Sergio Enrique
author Montel Mendoza, María Gabriela
author_facet Montel Mendoza, María Gabriela
Ale, Cesar Emmanuel
Nader, Maria Elena Fatima
Pasteris, Sergio Enrique
author_role author
author2 Ale, Cesar Emmanuel
Nader, Maria Elena Fatima
Pasteris, Sergio Enrique
author2_role author
author
author
dc.subject.none.fl_str_mv ENTEROCOCCUS GALLINARUM
RANICULTURE
BACTERIOCIN
PROBIOTICS
BIOPRESERVATIVES
topic ENTEROCOCCUS GALLINARUM
RANICULTURE
BACTERIOCIN
PROBIOTICS
BIOPRESERVATIVES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Enterococcus gallinarum CRL 1826 isolated from an American bullfrog (Lithobates catesbeianus) skin inhibits the growth of Citrobacter freundii, Pseudomonas aeruginosa (bullfrog pathogens) and Listeria monocytogenes by a synergistic effect between organic acids and a bacteriocin-like molecule. This bacteriocin, named enterocin CRL 1826, showed a proteinaceous nature, heat stability and polar characteristics. Its production followed kinetics of primary metabolites synthesis reaching a maximum of 61,400 AU/mL. The minimum inhibitory and minimumbactericidal concentrations were 2,640 and 5,280 AU/mL, respectively, against L. monocytogenes. The addition of 120,000 AU/mL of enterocin to growing L. monocytogenes and Gram-negative (P. aeruginosa and C. freundii) bacteria showed bactericidal and bacteriostatic effects, respectively. However, enterocin derived-peptides had bactericidal effect only against Gram-negatives.Enterocin produced cell envelope damages and efflux of citosolic content on L. monocytogenes, while enterocin derived-peptides showed granulation and contraction of cytoplasm material on P. aeruginosa and increase in theperiplasmic space and empty cells appearance on C. freundii.Enterocin CRL 1826 is the first bacteriocin described for E. gallinarum from raniculture. It could be used as a biopreservative while the derived-peptides represent an alternative to control multi-drug resistant Gram-negatives.The antimicrobial spectrum and the stability of enterocin and its derived-peptides indicate that they could be applied in different biotechnological areas.
Fil: Montel Mendoza, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Ale, Cesar Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
description Enterococcus gallinarum CRL 1826 isolated from an American bullfrog (Lithobates catesbeianus) skin inhibits the growth of Citrobacter freundii, Pseudomonas aeruginosa (bullfrog pathogens) and Listeria monocytogenes by a synergistic effect between organic acids and a bacteriocin-like molecule. This bacteriocin, named enterocin CRL 1826, showed a proteinaceous nature, heat stability and polar characteristics. Its production followed kinetics of primary metabolites synthesis reaching a maximum of 61,400 AU/mL. The minimum inhibitory and minimumbactericidal concentrations were 2,640 and 5,280 AU/mL, respectively, against L. monocytogenes. The addition of 120,000 AU/mL of enterocin to growing L. monocytogenes and Gram-negative (P. aeruginosa and C. freundii) bacteria showed bactericidal and bacteriostatic effects, respectively. However, enterocin derived-peptides had bactericidal effect only against Gram-negatives.Enterocin produced cell envelope damages and efflux of citosolic content on L. monocytogenes, while enterocin derived-peptides showed granulation and contraction of cytoplasm material on P. aeruginosa and increase in theperiplasmic space and empty cells appearance on C. freundii.Enterocin CRL 1826 is the first bacteriocin described for E. gallinarum from raniculture. It could be used as a biopreservative while the derived-peptides represent an alternative to control multi-drug resistant Gram-negatives.The antimicrobial spectrum and the stability of enterocin and its derived-peptides indicate that they could be applied in different biotechnological areas.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28259
Montel Mendoza, María Gabriela; Ale, Cesar Emmanuel; Nader, Maria Elena Fatima; Pasteris, Sergio Enrique; Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives; Omics International; Journal of Bioprocessing & Biotechniques; 5; 8; 24-8-2015; 1-9;1000250
2155-9821
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28259
identifier_str_mv Montel Mendoza, María Gabriela; Ale, Cesar Emmanuel; Nader, Maria Elena Fatima; Pasteris, Sergio Enrique; Characterization of a bacteriocin produced by enterococcus gallinarum CRL 1826 Isolated from captive bullfrog: evaluation of its mode of action against Listeria monocytogenes and gram-negatives; Omics International; Journal of Bioprocessing & Biotechniques; 5; 8; 24-8-2015; 1-9;1000250
2155-9821
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9821.1000250
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/characterization-of-a-bacteriocin-produced-by-enterococcus-gallinarum-crl-1826-isolated-from-captive-bullfrog-evaluation-of-its-mode-of-action-against-listeria-monocytogenes-and-gramnegatives-2155-9821-1000250.php?aid=58843
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Omics International
publisher.none.fl_str_mv Omics International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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