Silicates characterization as potential bacteriocin-carriers

Autores
Ibarguren, Carolina; Audisio, Marcela Carina; Farfan Torres, Elsa Monica; Apella, Maria Cristina
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two different silicates, zeosil and expanded perlite, were characterized as potential carriers of a bacteriocin with anti-Listeria monocytogenes activity, produced by Enterococcus faecium CRL1385. Specific surface areas showed a value significantly higher for zeosil (146 m2 g- 1) than for perlite (0.65 m2 g- 1). Potential zeta measurements revealed that both silicates had negatively charged surfaces between pH 2 and 11, but zeosil presented zero charge near pH 2. Fourier Transform Infrared (FTIR) spectra proved that zeosil presented more silanol groups available for bacteriocin interaction than perlite. Bacteriocins present in the cell-free supernatant (CFS) were adsorbed by both silicates. Adsorption was highest from pH 4 to 8 and, regardless of exposure time (0.5 or 4 h) and silicate concentration (1 or 4% w/v) at 25 °C. Bacteriocin adsorption onto zeosil (ca. 99%) was higher than onto expanded perlite (ca 80%). However, antimicrobial activity of bacteriocins adsorbed onto perlite was higher than onto zeosil. After 2 h contact between L. monocytogenes 01/155 cells and each silicate plus the bacteriocin sample, the number of Listeria viable cells decreased close to 2 and 6 log orders for zeosil and expanded perlite, respectively. Industrial relevance: One of the crucial problems in the use of bacteriocins as food biopreservatives is obtaining and purifying these antimicrobials. The process generally has a poor yield and is industrially expensive. Hence, alternative techniques to deliver bacteriocins may be a likely option to encourage their use as bioprotectors. Silicates, inert compounds of large surface area, are suggested in this work as peptide immobilizers so that they may later be used in food. These inorganic compounds have already been authorized as food-grade anticaking, clarifying or filtering agents. The results achieved so far with adsorption and anti-Listeria activity preservation of bacteriocin, once they have been immobilized onto silicates, offer a promising and simple alternative to incorporate this compound into food.
Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Farfan Torres, Elsa Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Materia
Bacteriocin
Food Biopreservatives
Listeria Monocytogenes
Silicates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78258
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78258
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Silicates characterization as potential bacteriocin-carriersIbarguren, CarolinaAudisio, Marcela CarinaFarfan Torres, Elsa MonicaApella, Maria CristinaBacteriocinFood BiopreservativesListeria MonocytogenesSilicateshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Two different silicates, zeosil and expanded perlite, were characterized as potential carriers of a bacteriocin with anti-Listeria monocytogenes activity, produced by Enterococcus faecium CRL1385. Specific surface areas showed a value significantly higher for zeosil (146 m2 g- 1) than for perlite (0.65 m2 g- 1). Potential zeta measurements revealed that both silicates had negatively charged surfaces between pH 2 and 11, but zeosil presented zero charge near pH 2. Fourier Transform Infrared (FTIR) spectra proved that zeosil presented more silanol groups available for bacteriocin interaction than perlite. Bacteriocins present in the cell-free supernatant (CFS) were adsorbed by both silicates. Adsorption was highest from pH 4 to 8 and, regardless of exposure time (0.5 or 4 h) and silicate concentration (1 or 4% w/v) at 25 °C. Bacteriocin adsorption onto zeosil (ca. 99%) was higher than onto expanded perlite (ca 80%). However, antimicrobial activity of bacteriocins adsorbed onto perlite was higher than onto zeosil. After 2 h contact between L. monocytogenes 01/155 cells and each silicate plus the bacteriocin sample, the number of Listeria viable cells decreased close to 2 and 6 log orders for zeosil and expanded perlite, respectively. Industrial relevance: One of the crucial problems in the use of bacteriocins as food biopreservatives is obtaining and purifying these antimicrobials. The process generally has a poor yield and is industrially expensive. Hence, alternative techniques to deliver bacteriocins may be a likely option to encourage their use as bioprotectors. Silicates, inert compounds of large surface area, are suggested in this work as peptide immobilizers so that they may later be used in food. These inorganic compounds have already been authorized as food-grade anticaking, clarifying or filtering agents. The results achieved so far with adsorption and anti-Listeria activity preservation of bacteriocin, once they have been immobilized onto silicates, offer a promising and simple alternative to incorporate this compound into food.Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Farfan Torres, Elsa Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaElsevier2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78258Ibarguren, Carolina; Audisio, Marcela Carina; Farfan Torres, Elsa Monica; Apella, Maria Cristina; Silicates characterization as potential bacteriocin-carriers; Elsevier; Innovative Food Science & Emerging Technologies; 11; 1; 1-2010; 197-2021466-85641878-5522CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ifset.2009.10.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1466856409001179info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:46Zoai:ri.conicet.gov.ar:11336/78258instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:46.636CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Silicates characterization as potential bacteriocin-carriers
title Silicates characterization as potential bacteriocin-carriers
spellingShingle Silicates characterization as potential bacteriocin-carriers
Ibarguren, Carolina
Bacteriocin
Food Biopreservatives
Listeria Monocytogenes
Silicates
title_short Silicates characterization as potential bacteriocin-carriers
title_full Silicates characterization as potential bacteriocin-carriers
title_fullStr Silicates characterization as potential bacteriocin-carriers
title_full_unstemmed Silicates characterization as potential bacteriocin-carriers
title_sort Silicates characterization as potential bacteriocin-carriers
dc.creator.none.fl_str_mv Ibarguren, Carolina
Audisio, Marcela Carina
Farfan Torres, Elsa Monica
Apella, Maria Cristina
author Ibarguren, Carolina
author_facet Ibarguren, Carolina
Audisio, Marcela Carina
Farfan Torres, Elsa Monica
Apella, Maria Cristina
author_role author
author2 Audisio, Marcela Carina
Farfan Torres, Elsa Monica
Apella, Maria Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv Bacteriocin
Food Biopreservatives
Listeria Monocytogenes
Silicates
topic Bacteriocin
Food Biopreservatives
Listeria Monocytogenes
Silicates
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Two different silicates, zeosil and expanded perlite, were characterized as potential carriers of a bacteriocin with anti-Listeria monocytogenes activity, produced by Enterococcus faecium CRL1385. Specific surface areas showed a value significantly higher for zeosil (146 m2 g- 1) than for perlite (0.65 m2 g- 1). Potential zeta measurements revealed that both silicates had negatively charged surfaces between pH 2 and 11, but zeosil presented zero charge near pH 2. Fourier Transform Infrared (FTIR) spectra proved that zeosil presented more silanol groups available for bacteriocin interaction than perlite. Bacteriocins present in the cell-free supernatant (CFS) were adsorbed by both silicates. Adsorption was highest from pH 4 to 8 and, regardless of exposure time (0.5 or 4 h) and silicate concentration (1 or 4% w/v) at 25 °C. Bacteriocin adsorption onto zeosil (ca. 99%) was higher than onto expanded perlite (ca 80%). However, antimicrobial activity of bacteriocins adsorbed onto perlite was higher than onto zeosil. After 2 h contact between L. monocytogenes 01/155 cells and each silicate plus the bacteriocin sample, the number of Listeria viable cells decreased close to 2 and 6 log orders for zeosil and expanded perlite, respectively. Industrial relevance: One of the crucial problems in the use of bacteriocins as food biopreservatives is obtaining and purifying these antimicrobials. The process generally has a poor yield and is industrially expensive. Hence, alternative techniques to deliver bacteriocins may be a likely option to encourage their use as bioprotectors. Silicates, inert compounds of large surface area, are suggested in this work as peptide immobilizers so that they may later be used in food. These inorganic compounds have already been authorized as food-grade anticaking, clarifying or filtering agents. The results achieved so far with adsorption and anti-Listeria activity preservation of bacteriocin, once they have been immobilized onto silicates, offer a promising and simple alternative to incorporate this compound into food.
Fil: Ibarguren, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Farfan Torres, Elsa Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
description Two different silicates, zeosil and expanded perlite, were characterized as potential carriers of a bacteriocin with anti-Listeria monocytogenes activity, produced by Enterococcus faecium CRL1385. Specific surface areas showed a value significantly higher for zeosil (146 m2 g- 1) than for perlite (0.65 m2 g- 1). Potential zeta measurements revealed that both silicates had negatively charged surfaces between pH 2 and 11, but zeosil presented zero charge near pH 2. Fourier Transform Infrared (FTIR) spectra proved that zeosil presented more silanol groups available for bacteriocin interaction than perlite. Bacteriocins present in the cell-free supernatant (CFS) were adsorbed by both silicates. Adsorption was highest from pH 4 to 8 and, regardless of exposure time (0.5 or 4 h) and silicate concentration (1 or 4% w/v) at 25 °C. Bacteriocin adsorption onto zeosil (ca. 99%) was higher than onto expanded perlite (ca 80%). However, antimicrobial activity of bacteriocins adsorbed onto perlite was higher than onto zeosil. After 2 h contact between L. monocytogenes 01/155 cells and each silicate plus the bacteriocin sample, the number of Listeria viable cells decreased close to 2 and 6 log orders for zeosil and expanded perlite, respectively. Industrial relevance: One of the crucial problems in the use of bacteriocins as food biopreservatives is obtaining and purifying these antimicrobials. The process generally has a poor yield and is industrially expensive. Hence, alternative techniques to deliver bacteriocins may be a likely option to encourage their use as bioprotectors. Silicates, inert compounds of large surface area, are suggested in this work as peptide immobilizers so that they may later be used in food. These inorganic compounds have already been authorized as food-grade anticaking, clarifying or filtering agents. The results achieved so far with adsorption and anti-Listeria activity preservation of bacteriocin, once they have been immobilized onto silicates, offer a promising and simple alternative to incorporate this compound into food.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78258
Ibarguren, Carolina; Audisio, Marcela Carina; Farfan Torres, Elsa Monica; Apella, Maria Cristina; Silicates characterization as potential bacteriocin-carriers; Elsevier; Innovative Food Science & Emerging Technologies; 11; 1; 1-2010; 197-202
1466-8564
1878-5522
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78258
identifier_str_mv Ibarguren, Carolina; Audisio, Marcela Carina; Farfan Torres, Elsa Monica; Apella, Maria Cristina; Silicates characterization as potential bacteriocin-carriers; Elsevier; Innovative Food Science & Emerging Technologies; 11; 1; 1-2010; 197-202
1466-8564
1878-5522
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ifset.2009.10.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1466856409001179
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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