Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds

Autores
Cardoso, Maria de Las Mercedes; Manzo, Ricardo Martín; Tonarelli, Georgina Guadalupe; Simonetta, Arturo Carlos
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enterococcus faecalis DBFIQ E24 strain produces antimicrobial substances that inhibit the growth of
food-borne pathogenic bacteria, yeasts and moulds. This broad antimicrobial spectrum was determined by obtaining the cell-free supernatant (CFS) and employing the agar-well-diffusion method. Its antibacterial activity was completely inactivated by the action of proteinase K and not affected by catalase action. Besides, it was stable to thermal treatments, showing maximum antibacterial activity at pH 7.0. Moreover, CFS showed a bactericidal effect against Escherichia coli DBFIQ Ec9 strain. All these interesting physicochemical properties allow that either CFS or E. faecalis DBFIQ E24 strain to be considered as potential food biopreservatives.
Fil: Cardoso, Maria de Las Mercedes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Materia
Bacteriocin
Enterococcus Faecalis Strain
Cell Free Supernatant
Characterisation
Food Biopreservative
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24201
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24201
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and mouldsCardoso, Maria de Las MercedesManzo, Ricardo MartínTonarelli, Georgina GuadalupeSimonetta, Arturo CarlosBacteriocinEnterococcus Faecalis StrainCell Free SupernatantCharacterisationFood Biopreservativehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Enterococcus faecalis DBFIQ E24 strain produces antimicrobial substances that inhibit the growth of<br />food-borne pathogenic bacteria, yeasts and moulds. This broad antimicrobial spectrum was determined by obtaining the cell-free supernatant (CFS) and employing the agar-well-diffusion method. Its antibacterial activity was completely inactivated by the action of proteinase K and not affected by catalase action. Besides, it was stable to thermal treatments, showing maximum antibacterial activity at pH 7.0. Moreover, CFS showed a bactericidal effect against Escherichia coli DBFIQ Ec9 strain. All these interesting physicochemical properties allow that either CFS or E. faecalis DBFIQ E24 strain to be considered as potential food biopreservatives.Fil: Cardoso, Maria de Las Mercedes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaElsevier2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24201Cardoso, Maria de Las Mercedes; Manzo, Ricardo Martín; Tonarelli, Georgina Guadalupe; Simonetta, Arturo Carlos; Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds; Elsevier; International Journal Of Dairy Technology; 65; 4; 11-2012; 568-5771364-727XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.springer.com/chemistry/biotechnology/journal/11274info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-0307.2012.00852.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:35Zoai:ri.conicet.gov.ar:11336/24201instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:36.035CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
title Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
spellingShingle Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
Cardoso, Maria de Las Mercedes
Bacteriocin
Enterococcus Faecalis Strain
Cell Free Supernatant
Characterisation
Food Biopreservative
title_short Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
title_full Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
title_fullStr Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
title_full_unstemmed Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
title_sort Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds
dc.creator.none.fl_str_mv Cardoso, Maria de Las Mercedes
Manzo, Ricardo Martín
Tonarelli, Georgina Guadalupe
Simonetta, Arturo Carlos
author Cardoso, Maria de Las Mercedes
author_facet Cardoso, Maria de Las Mercedes
Manzo, Ricardo Martín
Tonarelli, Georgina Guadalupe
Simonetta, Arturo Carlos
author_role author
author2 Manzo, Ricardo Martín
Tonarelli, Georgina Guadalupe
Simonetta, Arturo Carlos
author2_role author
author
author
dc.subject.none.fl_str_mv Bacteriocin
Enterococcus Faecalis Strain
Cell Free Supernatant
Characterisation
Food Biopreservative
topic Bacteriocin
Enterococcus Faecalis Strain
Cell Free Supernatant
Characterisation
Food Biopreservative
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Enterococcus faecalis DBFIQ E24 strain produces antimicrobial substances that inhibit the growth of<br />food-borne pathogenic bacteria, yeasts and moulds. This broad antimicrobial spectrum was determined by obtaining the cell-free supernatant (CFS) and employing the agar-well-diffusion method. Its antibacterial activity was completely inactivated by the action of proteinase K and not affected by catalase action. Besides, it was stable to thermal treatments, showing maximum antibacterial activity at pH 7.0. Moreover, CFS showed a bactericidal effect against Escherichia coli DBFIQ Ec9 strain. All these interesting physicochemical properties allow that either CFS or E. faecalis DBFIQ E24 strain to be considered as potential food biopreservatives.
Fil: Cardoso, Maria de Las Mercedes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Tonarelli, Georgina Guadalupe. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Simonetta, Arturo Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
description Enterococcus faecalis DBFIQ E24 strain produces antimicrobial substances that inhibit the growth of<br />food-borne pathogenic bacteria, yeasts and moulds. This broad antimicrobial spectrum was determined by obtaining the cell-free supernatant (CFS) and employing the agar-well-diffusion method. Its antibacterial activity was completely inactivated by the action of proteinase K and not affected by catalase action. Besides, it was stable to thermal treatments, showing maximum antibacterial activity at pH 7.0. Moreover, CFS showed a bactericidal effect against Escherichia coli DBFIQ Ec9 strain. All these interesting physicochemical properties allow that either CFS or E. faecalis DBFIQ E24 strain to be considered as potential food biopreservatives.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24201
Cardoso, Maria de Las Mercedes; Manzo, Ricardo Martín; Tonarelli, Georgina Guadalupe; Simonetta, Arturo Carlos; Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds; Elsevier; International Journal Of Dairy Technology; 65; 4; 11-2012; 568-577
1364-727X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24201
identifier_str_mv Cardoso, Maria de Las Mercedes; Manzo, Ricardo Martín; Tonarelli, Georgina Guadalupe; Simonetta, Arturo Carlos; Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds; Elsevier; International Journal Of Dairy Technology; 65; 4; 11-2012; 568-577
1364-727X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.springer.com/chemistry/biotechnology/journal/11274
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-0307.2012.00852.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269235476168704
score 13.13397

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