Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii

Autores
Labrador Herrera, Gema; Pérez Pulido, Antonio J.; Álvarez Marín, Rocío; Casimiro Soriguer, Carlos S.; Cebrero Cangueiro, Tania; Moran Barrio, Jorgelina; Pachón, Jerónimo; Viale, Alejandro Miguel; Pachón Ibáñez, María Eugenia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.
Fil: Labrador Herrera, Gema. Universidad de Sevilla; España. Hospital Universitario Virgen del Rocío; España
Fil: Pérez Pulido, Antonio J.. Universidad Pablo de Olavide; España
Fil: Álvarez Marín, Rocío. Hospital Universitario Virgen del Rocío; España. Universidad de Sevilla; España
Fil: Casimiro Soriguer, Carlos S.. Universidad Pablo de Olavide; España
Fil: Cebrero Cangueiro, Tania. Universidad de Sevilla; España. Hospital Universitario Virgen del Rocío; España
Fil: Moran Barrio, Jorgelina. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Pachón, Jerónimo. Universidad de Sevilla; España
Fil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Pachón Ibáñez, María Eugenia. Hospital Universitario Virgen del Rocío; España. Universidad de Sevilla; España
Materia
ACINETOBACTER BAUMANNII
BLOODSTREAM INFECTIONS
CARBAPENEM-RESISTANCE
CARO
VIRULENCE FACTOR
WHOLE-GENOME SEQUENCING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184733
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184733
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumanniiLabrador Herrera, GemaPérez Pulido, Antonio J.Álvarez Marín, RocíoCasimiro Soriguer, Carlos S.Cebrero Cangueiro, TaniaMoran Barrio, JorgelinaPachón, JerónimoViale, Alejandro MiguelPachón Ibáñez, María EugeniaACINETOBACTER BAUMANNIIBLOODSTREAM INFECTIONSCARBAPENEM-RESISTANCECAROVIRULENCE FACTORWHOLE-GENOME SEQUENCINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.Fil: Labrador Herrera, Gema. Universidad de Sevilla; España. Hospital Universitario Virgen del Rocío; EspañaFil: Pérez Pulido, Antonio J.. Universidad Pablo de Olavide; EspañaFil: Álvarez Marín, Rocío. Hospital Universitario Virgen del Rocío; España. Universidad de Sevilla; EspañaFil: Casimiro Soriguer, Carlos S.. Universidad Pablo de Olavide; EspañaFil: Cebrero Cangueiro, Tania. Universidad de Sevilla; España. Hospital Universitario Virgen del Rocío; EspañaFil: Moran Barrio, Jorgelina. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Pachón, Jerónimo. Universidad de Sevilla; EspañaFil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Pachón Ibáñez, María Eugenia. Hospital Universitario Virgen del Rocío; España. Universidad de Sevilla; EspañaBellwether Publishing Ltd2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184733Labrador Herrera, Gema; Pérez Pulido, Antonio J.; Álvarez Marín, Rocío; Casimiro Soriguer, Carlos S.; Cebrero Cangueiro, Tania; et al.; Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii; Bellwether Publishing Ltd; Virulence; 11; 1; 12-2020; 1727-17372150-55942150-5608CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/21505594.2020.1855912info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/21505594.2020.1855912info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:08Zoai:ri.conicet.gov.ar:11336/184733instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:08.618CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
spellingShingle Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
Labrador Herrera, Gema
ACINETOBACTER BAUMANNII
BLOODSTREAM INFECTIONS
CARBAPENEM-RESISTANCE
CARO
VIRULENCE FACTOR
WHOLE-GENOME SEQUENCING
title_short Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_full Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_fullStr Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_full_unstemmed Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
title_sort Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
dc.creator.none.fl_str_mv Labrador Herrera, Gema
Pérez Pulido, Antonio J.
Álvarez Marín, Rocío
Casimiro Soriguer, Carlos S.
Cebrero Cangueiro, Tania
Moran Barrio, Jorgelina
Pachón, Jerónimo
Viale, Alejandro Miguel
Pachón Ibáñez, María Eugenia
author Labrador Herrera, Gema
author_facet Labrador Herrera, Gema
Pérez Pulido, Antonio J.
Álvarez Marín, Rocío
Casimiro Soriguer, Carlos S.
Cebrero Cangueiro, Tania
Moran Barrio, Jorgelina
Pachón, Jerónimo
Viale, Alejandro Miguel
Pachón Ibáñez, María Eugenia
author_role author
author2 Pérez Pulido, Antonio J.
Álvarez Marín, Rocío
Casimiro Soriguer, Carlos S.
Cebrero Cangueiro, Tania
Moran Barrio, Jorgelina
Pachón, Jerónimo
Viale, Alejandro Miguel
Pachón Ibáñez, María Eugenia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
BLOODSTREAM INFECTIONS
CARBAPENEM-RESISTANCE
CARO
VIRULENCE FACTOR
WHOLE-GENOME SEQUENCING
topic ACINETOBACTER BAUMANNII
BLOODSTREAM INFECTIONS
CARBAPENEM-RESISTANCE
CARO
VIRULENCE FACTOR
WHOLE-GENOME SEQUENCING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.
Fil: Labrador Herrera, Gema. Universidad de Sevilla; España. Hospital Universitario Virgen del Rocío; España
Fil: Pérez Pulido, Antonio J.. Universidad Pablo de Olavide; España
Fil: Álvarez Marín, Rocío. Hospital Universitario Virgen del Rocío; España. Universidad de Sevilla; España
Fil: Casimiro Soriguer, Carlos S.. Universidad Pablo de Olavide; España
Fil: Cebrero Cangueiro, Tania. Universidad de Sevilla; España. Hospital Universitario Virgen del Rocío; España
Fil: Moran Barrio, Jorgelina. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Pachón, Jerónimo. Universidad de Sevilla; España
Fil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Pachón Ibáñez, María Eugenia. Hospital Universitario Virgen del Rocío; España. Universidad de Sevilla; España
description Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184733
Labrador Herrera, Gema; Pérez Pulido, Antonio J.; Álvarez Marín, Rocío; Casimiro Soriguer, Carlos S.; Cebrero Cangueiro, Tania; et al.; Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii; Bellwether Publishing Ltd; Virulence; 11; 1; 12-2020; 1727-1737
2150-5594
2150-5608
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184733
identifier_str_mv Labrador Herrera, Gema; Pérez Pulido, Antonio J.; Álvarez Marín, Rocío; Casimiro Soriguer, Carlos S.; Cebrero Cangueiro, Tania; et al.; Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii; Bellwether Publishing Ltd; Virulence; 11; 1; 12-2020; 1727-1737
2150-5594
2150-5608
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/21505594.2020.1855912
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/21505594.2020.1855912
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bellwether Publishing Ltd
publisher.none.fl_str_mv Bellwether Publishing Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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