The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii

Autores
Huang, Fanny; Fitchett, Noelle; Razo Gutierrez, Chelsea; Le, Casin; Martinez, Jasmine; Ra, Grace; Lopez, Carolina; Gonzalez, Lisandro Javier; Sieira, Rodrigo; Vila, Alejandro Jose; Bonomo, Robert A.; Ramirez, Maria Soledad
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems.
Fil: Huang, Fanny. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Fitchett, Noelle. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Razo Gutierrez, Chelsea. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Le, Casin. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Martinez, Jasmine. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Ra, Grace. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Lopez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gonzalez, Lisandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Va Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University. College Of Natural Science And Mathematics; Estados Unidos
Materia
ACINETOBACTER
ACINETOBACTER BAUMANNII
CARBAPENEMASES
CARBAPENEMS
H-NS
STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184729
Acceder
id CONICETDig_7a74041e34bca76e74c074798d33ff78
oai_identifier_str oai:ri.conicet.gov.ar:11336/184729
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumanniiHuang, FannyFitchett, NoelleRazo Gutierrez, ChelseaLe, CasinMartinez, JasmineRa, GraceLopez, CarolinaGonzalez, Lisandro JavierSieira, RodrigoVila, Alejandro JoseBonomo, Robert A.Ramirez, Maria SoledadACINETOBACTERACINETOBACTER BAUMANNIICARBAPENEMASESCARBAPENEMSH-NSSTRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems.Fil: Huang, Fanny. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: Fitchett, Noelle. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: Razo Gutierrez, Chelsea. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: Le, Casin. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: Martinez, Jasmine. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: Ra, Grace. California State University. College Of Natural Science And Mathematics; Estados UnidosFil: Lopez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gonzalez, Lisandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Bonomo, Robert A.. Louis Stokes Cleveland Va Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados UnidosFil: Ramirez, Maria Soledad. California State University. College Of Natural Science And Mathematics; Estados UnidosAmerican Society for Microbiology2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184729Huang, Fanny; Fitchett, Noelle; Razo Gutierrez, Chelsea; Le, Casin; Martinez, Jasmine; et al.; The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii; American Society for Microbiology; mSphere; 5; 4; 8-2020; 1-62379-5042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://msphere.asm.org/content/5/4/e00793-20info:eu-repo/semantics/altIdentifier/doi/10.1128/mSphere.00793-20info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:45Zoai:ri.conicet.gov.ar:11336/184729instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:45.808CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
title The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
spellingShingle The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
Huang, Fanny
ACINETOBACTER
ACINETOBACTER BAUMANNII
CARBAPENEMASES
CARBAPENEMS
H-NS
STRESS
title_short The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
title_full The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
title_fullStr The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
title_full_unstemmed The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
title_sort The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii
dc.creator.none.fl_str_mv Huang, Fanny
Fitchett, Noelle
Razo Gutierrez, Chelsea
Le, Casin
Martinez, Jasmine
Ra, Grace
Lopez, Carolina
Gonzalez, Lisandro Javier
Sieira, Rodrigo
Vila, Alejandro Jose
Bonomo, Robert A.
Ramirez, Maria Soledad
author Huang, Fanny
author_facet Huang, Fanny
Fitchett, Noelle
Razo Gutierrez, Chelsea
Le, Casin
Martinez, Jasmine
Ra, Grace
Lopez, Carolina
Gonzalez, Lisandro Javier
Sieira, Rodrigo
Vila, Alejandro Jose
Bonomo, Robert A.
Ramirez, Maria Soledad
author_role author
author2 Fitchett, Noelle
Razo Gutierrez, Chelsea
Le, Casin
Martinez, Jasmine
Ra, Grace
Lopez, Carolina
Gonzalez, Lisandro Javier
Sieira, Rodrigo
Vila, Alejandro Jose
Bonomo, Robert A.
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER
ACINETOBACTER BAUMANNII
CARBAPENEMASES
CARBAPENEMS
H-NS
STRESS
topic ACINETOBACTER
ACINETOBACTER BAUMANNII
CARBAPENEMASES
CARBAPENEMS
H-NS
STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems.
Fil: Huang, Fanny. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Fitchett, Noelle. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Razo Gutierrez, Chelsea. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Le, Casin. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Martinez, Jasmine. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Ra, Grace. California State University. College Of Natural Science And Mathematics; Estados Unidos
Fil: Lopez, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gonzalez, Lisandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Va Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University. College Of Natural Science And Mathematics; Estados Unidos
description Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184729
Huang, Fanny; Fitchett, Noelle; Razo Gutierrez, Chelsea; Le, Casin; Martinez, Jasmine; et al.; The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii; American Society for Microbiology; mSphere; 5; 4; 8-2020; 1-6
2379-5042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184729
identifier_str_mv Huang, Fanny; Fitchett, Noelle; Razo Gutierrez, Chelsea; Le, Casin; Martinez, Jasmine; et al.; The H-NS regulator plays a role in the stress induced by carbapenemase expression in acinetobacter baumannii; American Society for Microbiology; mSphere; 5; 4; 8-2020; 1-6
2379-5042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://msphere.asm.org/content/5/4/e00793-20
info:eu-repo/semantics/altIdentifier/doi/10.1128/mSphere.00793-20
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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