Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior

Autores
Fernandez, Jennifer S.; Tuttobene, Marisel Romina; Montaña, Sabrina Daiana; Subils, Tomás; Cantera, Virginia; Iriarte, Andres; Tuchscherr, Lorena; Ramirez, Maria Soledad
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polymicrobial infections are more challenging to treat and are recognized as responsible for significant morbidity and mortality. It has been demonstrated that multiple Gram-negative organisms take advantage of the effects of Staphylococcus aureus α-toxin on mucosal host defense, resulting in proliferation and dissemination of the co-infecting pathogens. Through phenotypic approaches, we observed a decrease in the motility of A. baumannii A118 after exposure to cell-free conditioned media (CFCM) of S. aureus strains, USA300 and LS1. However, the motility of A. baumannii A118 was increased after exposure to the CFCM of S. aureus strains USA300 ∆hla and S. aureus LSI ∆agrA. Hemolytic activity was seen in A118, in the presence of CFCM of S. aureus LS1. Further, A. baumannii A118 showed an increase in biofilm formation and antibiotic resistance to tetracycline, in the presence of CFCM of S. aureus USA300. Transcriptomic analysis of A. baumannii A118, with the addition of CFCM from S. aureus USA300, was carried out to study A. baumannii response to S. aureus’ released molecules. The RNA-seq data analysis showed a total of 463 differentially expressed genes, associated with a wide variety of functions, such as biofilm formation, virulence, and antibiotic susceptibility, among others. The present results showed that A. baumannii can sense and respond to molecules secreted by S. aureus. These findings demonstrate that A. baumannii may perceive and respond to changes in its environment; specifically, when in the presence of CFCM from S. aureus.
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Montaña, Sabrina Daiana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Cantera, Virginia. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; Uruguay
Fil: Iriarte, Andres. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; Uruguay
Fil: Tuchscherr, Lorena. Jena University Hospital; Alemania
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
Materia
ACINETOBACTER BAUMANNII
COMMENSALS
POLYMICROBIAL INFECTIONS
STAPHYLOCOCCUS AUREUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210420
Acceder
id CONICETDig_36d9fce312a361702e9c0d286201460b
oai_identifier_str oai:ri.conicet.gov.ar:11336/210420
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii BehaviorFernandez, Jennifer S.Tuttobene, Marisel RominaMontaña, Sabrina DaianaSubils, TomásCantera, VirginiaIriarte, AndresTuchscherr, LorenaRamirez, Maria SoledadACINETOBACTER BAUMANNIICOMMENSALSPOLYMICROBIAL INFECTIONSSTAPHYLOCOCCUS AUREUShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Polymicrobial infections are more challenging to treat and are recognized as responsible for significant morbidity and mortality. It has been demonstrated that multiple Gram-negative organisms take advantage of the effects of Staphylococcus aureus α-toxin on mucosal host defense, resulting in proliferation and dissemination of the co-infecting pathogens. Through phenotypic approaches, we observed a decrease in the motility of A. baumannii A118 after exposure to cell-free conditioned media (CFCM) of S. aureus strains, USA300 and LS1. However, the motility of A. baumannii A118 was increased after exposure to the CFCM of S. aureus strains USA300 ∆hla and S. aureus LSI ∆agrA. Hemolytic activity was seen in A118, in the presence of CFCM of S. aureus LS1. Further, A. baumannii A118 showed an increase in biofilm formation and antibiotic resistance to tetracycline, in the presence of CFCM of S. aureus USA300. Transcriptomic analysis of A. baumannii A118, with the addition of CFCM from S. aureus USA300, was carried out to study A. baumannii response to S. aureus’ released molecules. The RNA-seq data analysis showed a total of 463 differentially expressed genes, associated with a wide variety of functions, such as biofilm formation, virulence, and antibiotic susceptibility, among others. The present results showed that A. baumannii can sense and respond to molecules secreted by S. aureus. These findings demonstrate that A. baumannii may perceive and respond to changes in its environment; specifically, when in the presence of CFCM from S. aureus.Fil: Fernandez, Jennifer S.. California State University; Estados UnidosFil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Montaña, Sabrina Daiana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Cantera, Virginia. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; UruguayFil: Iriarte, Andres. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; UruguayFil: Tuchscherr, Lorena. Jena University Hospital; AlemaniaFil: Ramirez, Maria Soledad. California State University; Estados UnidosMDPI2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210420Fernandez, Jennifer S. ; Tuttobene, Marisel Romina; Montaña, Sabrina Daiana; Subils, Tomás; Cantera, Virginia; et al.; Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior; MDPI; Biology; 11; 4; 4-2022; 1-132079-7737CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/biology11040570info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:22Zoai:ri.conicet.gov.ar:11336/210420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:23.65CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
title Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
spellingShingle Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
Fernandez, Jennifer S.
ACINETOBACTER BAUMANNII
COMMENSALS
POLYMICROBIAL INFECTIONS
STAPHYLOCOCCUS AUREUS
title_short Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
title_full Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
title_fullStr Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
title_full_unstemmed Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
title_sort Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior
dc.creator.none.fl_str_mv Fernandez, Jennifer S.
Tuttobene, Marisel Romina
Montaña, Sabrina Daiana
Subils, Tomás
Cantera, Virginia
Iriarte, Andres
Tuchscherr, Lorena
Ramirez, Maria Soledad
author Fernandez, Jennifer S.
author_facet Fernandez, Jennifer S.
Tuttobene, Marisel Romina
Montaña, Sabrina Daiana
Subils, Tomás
Cantera, Virginia
Iriarte, Andres
Tuchscherr, Lorena
Ramirez, Maria Soledad
author_role author
author2 Tuttobene, Marisel Romina
Montaña, Sabrina Daiana
Subils, Tomás
Cantera, Virginia
Iriarte, Andres
Tuchscherr, Lorena
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
COMMENSALS
POLYMICROBIAL INFECTIONS
STAPHYLOCOCCUS AUREUS
topic ACINETOBACTER BAUMANNII
COMMENSALS
POLYMICROBIAL INFECTIONS
STAPHYLOCOCCUS AUREUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polymicrobial infections are more challenging to treat and are recognized as responsible for significant morbidity and mortality. It has been demonstrated that multiple Gram-negative organisms take advantage of the effects of Staphylococcus aureus α-toxin on mucosal host defense, resulting in proliferation and dissemination of the co-infecting pathogens. Through phenotypic approaches, we observed a decrease in the motility of A. baumannii A118 after exposure to cell-free conditioned media (CFCM) of S. aureus strains, USA300 and LS1. However, the motility of A. baumannii A118 was increased after exposure to the CFCM of S. aureus strains USA300 ∆hla and S. aureus LSI ∆agrA. Hemolytic activity was seen in A118, in the presence of CFCM of S. aureus LS1. Further, A. baumannii A118 showed an increase in biofilm formation and antibiotic resistance to tetracycline, in the presence of CFCM of S. aureus USA300. Transcriptomic analysis of A. baumannii A118, with the addition of CFCM from S. aureus USA300, was carried out to study A. baumannii response to S. aureus’ released molecules. The RNA-seq data analysis showed a total of 463 differentially expressed genes, associated with a wide variety of functions, such as biofilm formation, virulence, and antibiotic susceptibility, among others. The present results showed that A. baumannii can sense and respond to molecules secreted by S. aureus. These findings demonstrate that A. baumannii may perceive and respond to changes in its environment; specifically, when in the presence of CFCM from S. aureus.
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Montaña, Sabrina Daiana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Cantera, Virginia. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; Uruguay
Fil: Iriarte, Andres. Universidad de la Republica. Facultad de Medicina. Instituto de Higiene. Departamento de Desarrollo Biotecnológico.; Uruguay
Fil: Tuchscherr, Lorena. Jena University Hospital; Alemania
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
description Polymicrobial infections are more challenging to treat and are recognized as responsible for significant morbidity and mortality. It has been demonstrated that multiple Gram-negative organisms take advantage of the effects of Staphylococcus aureus α-toxin on mucosal host defense, resulting in proliferation and dissemination of the co-infecting pathogens. Through phenotypic approaches, we observed a decrease in the motility of A. baumannii A118 after exposure to cell-free conditioned media (CFCM) of S. aureus strains, USA300 and LS1. However, the motility of A. baumannii A118 was increased after exposure to the CFCM of S. aureus strains USA300 ∆hla and S. aureus LSI ∆agrA. Hemolytic activity was seen in A118, in the presence of CFCM of S. aureus LS1. Further, A. baumannii A118 showed an increase in biofilm formation and antibiotic resistance to tetracycline, in the presence of CFCM of S. aureus USA300. Transcriptomic analysis of A. baumannii A118, with the addition of CFCM from S. aureus USA300, was carried out to study A. baumannii response to S. aureus’ released molecules. The RNA-seq data analysis showed a total of 463 differentially expressed genes, associated with a wide variety of functions, such as biofilm formation, virulence, and antibiotic susceptibility, among others. The present results showed that A. baumannii can sense and respond to molecules secreted by S. aureus. These findings demonstrate that A. baumannii may perceive and respond to changes in its environment; specifically, when in the presence of CFCM from S. aureus.
publishDate 2022
dc.date.none.fl_str_mv 2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210420
Fernandez, Jennifer S. ; Tuttobene, Marisel Romina; Montaña, Sabrina Daiana; Subils, Tomás; Cantera, Virginia; et al.; Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior; MDPI; Biology; 11; 4; 4-2022; 1-13
2079-7737
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210420
identifier_str_mv Fernandez, Jennifer S. ; Tuttobene, Marisel Romina; Montaña, Sabrina Daiana; Subils, Tomás; Cantera, Virginia; et al.; Staphylococcus aureus α-Toxin Effect on Acinetobacter baumannii Behavior; MDPI; Biology; 11; 4; 4-2022; 1-13
2079-7737
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/biology11040570
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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