Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation

Autores
Le, Casin; Pimentel, Camila; Tuttobene, Marisel Romina; Subils, Tomás; Escalante, Jenny; Nishimura, Brent; Arriaga, Susana; Rodgers, Deja; Bonomo, Robert A.; Sieira, Rodrigo; Tolmasky, Marcelo E.; Ramírez, María Soledad
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Most Acinetobacter baumannii strains are naturally competent. Although some information is available about factors that enhance or reduce the frequency of the transformation of this bacterium, the regulatory elements and mechanisms are barely understood. In this article, we describe studies on the role of the histone-like nucleoid structuring protein, H-NS, in the regulation of the expression of genes related to natural competency and the ability to uptake foreign DNA. The expression levels of the natural transformation-related genes pilA, pilT, pilQ, comEA, comEC, comF, and drpA significantly increased in a ∆hns derivative of A. baumannii A118. The complementation of the mutant with a recombinant plasmid harboring hns restored the expression levels of six of these genes (pilT remained expressed at high levels) to those of the wild-type strain. The transformation frequency of the A. baumannii A118 ∆hns strain was significantly higher than that of the wild-type. Similar, albeit not identical, there were consequences when hns was deleted from the hypervirulent A. baumannii AB5075 strain. In the AB5075 complemented strain, the reduction in gene expression in a few cases was not so pronounced that it reached wild-type levels, and the expression of comEA was enhanced further. In conclusion, the expression of all seven transformation-related genes was enhanced after deleting hns in A. baumannii A118 and AB5075, and these modifications were accompanied by an increase in the cells’ transformability. The results highlight a role of H-NS in A. baumannii’s natural competence.
Fil: Le, Casin. California State University; Estados Unidos
Fil: Pimentel, Camila. California State University; Estados Unidos
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Nishimura, Brent. California State University; Estados Unidos
Fil: Arriaga, Susana. California State University; Estados Unidos
Fil: Rodgers, Deja. California State University; Estados Unidos
Fil: Bonomo, Robert A.. Center for Antimicrobial Resistance and Epidemiology; Estados Unidos. Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Ramírez, María Soledad. California State University; Estados Unidos
Materia
ACINETOBACTER BAUMANNII
DNA ACQUISITION
H-NS
NATURAL TRANSFORMATION
NATURALLY COMPETENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212025
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212025
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformationLe, CasinPimentel, CamilaTuttobene, Marisel RominaSubils, TomásEscalante, JennyNishimura, BrentArriaga, SusanaRodgers, DejaBonomo, Robert A.Sieira, RodrigoTolmasky, Marcelo E.Ramírez, María SoledadACINETOBACTER BAUMANNIIDNA ACQUISITIONH-NSNATURAL TRANSFORMATIONNATURALLY COMPETENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Most Acinetobacter baumannii strains are naturally competent. Although some information is available about factors that enhance or reduce the frequency of the transformation of this bacterium, the regulatory elements and mechanisms are barely understood. In this article, we describe studies on the role of the histone-like nucleoid structuring protein, H-NS, in the regulation of the expression of genes related to natural competency and the ability to uptake foreign DNA. The expression levels of the natural transformation-related genes pilA, pilT, pilQ, comEA, comEC, comF, and drpA significantly increased in a ∆hns derivative of A. baumannii A118. The complementation of the mutant with a recombinant plasmid harboring hns restored the expression levels of six of these genes (pilT remained expressed at high levels) to those of the wild-type strain. The transformation frequency of the A. baumannii A118 ∆hns strain was significantly higher than that of the wild-type. Similar, albeit not identical, there were consequences when hns was deleted from the hypervirulent A. baumannii AB5075 strain. In the AB5075 complemented strain, the reduction in gene expression in a few cases was not so pronounced that it reached wild-type levels, and the expression of comEA was enhanced further. In conclusion, the expression of all seven transformation-related genes was enhanced after deleting hns in A. baumannii A118 and AB5075, and these modifications were accompanied by an increase in the cells’ transformability. The results highlight a role of H-NS in A. baumannii’s natural competence.Fil: Le, Casin. California State University; Estados UnidosFil: Pimentel, Camila. California State University; Estados UnidosFil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Escalante, Jenny. California State University; Estados UnidosFil: Nishimura, Brent. California State University; Estados UnidosFil: Arriaga, Susana. California State University; Estados UnidosFil: Rodgers, Deja. California State University; Estados UnidosFil: Bonomo, Robert A.. Center for Antimicrobial Resistance and Epidemiology; Estados Unidos. Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados UnidosFil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Ramírez, María Soledad. California State University; Estados UnidosMultidisciplinary Digital Publishing Institute2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212025Le, Casin; Pimentel, Camila; Tuttobene, Marisel Romina; Subils, Tomás; Escalante, Jenny; et al.; Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation; Multidisciplinary Digital Publishing Institute; Pathogens; 10; 9; 9-2021; 1-72076-0817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/9/1083info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10091083info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:40Zoai:ri.conicet.gov.ar:11336/212025instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:40.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
title Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
spellingShingle Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
Le, Casin
ACINETOBACTER BAUMANNII
DNA ACQUISITION
H-NS
NATURAL TRANSFORMATION
NATURALLY COMPETENT
title_short Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
title_full Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
title_fullStr Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
title_full_unstemmed Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
title_sort Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation
dc.creator.none.fl_str_mv Le, Casin
Pimentel, Camila
Tuttobene, Marisel Romina
Subils, Tomás
Escalante, Jenny
Nishimura, Brent
Arriaga, Susana
Rodgers, Deja
Bonomo, Robert A.
Sieira, Rodrigo
Tolmasky, Marcelo E.
Ramírez, María Soledad
author Le, Casin
author_facet Le, Casin
Pimentel, Camila
Tuttobene, Marisel Romina
Subils, Tomás
Escalante, Jenny
Nishimura, Brent
Arriaga, Susana
Rodgers, Deja
Bonomo, Robert A.
Sieira, Rodrigo
Tolmasky, Marcelo E.
Ramírez, María Soledad
author_role author
author2 Pimentel, Camila
Tuttobene, Marisel Romina
Subils, Tomás
Escalante, Jenny
Nishimura, Brent
Arriaga, Susana
Rodgers, Deja
Bonomo, Robert A.
Sieira, Rodrigo
Tolmasky, Marcelo E.
Ramírez, María Soledad
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
DNA ACQUISITION
H-NS
NATURAL TRANSFORMATION
NATURALLY COMPETENT
topic ACINETOBACTER BAUMANNII
DNA ACQUISITION
H-NS
NATURAL TRANSFORMATION
NATURALLY COMPETENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Most Acinetobacter baumannii strains are naturally competent. Although some information is available about factors that enhance or reduce the frequency of the transformation of this bacterium, the regulatory elements and mechanisms are barely understood. In this article, we describe studies on the role of the histone-like nucleoid structuring protein, H-NS, in the regulation of the expression of genes related to natural competency and the ability to uptake foreign DNA. The expression levels of the natural transformation-related genes pilA, pilT, pilQ, comEA, comEC, comF, and drpA significantly increased in a ∆hns derivative of A. baumannii A118. The complementation of the mutant with a recombinant plasmid harboring hns restored the expression levels of six of these genes (pilT remained expressed at high levels) to those of the wild-type strain. The transformation frequency of the A. baumannii A118 ∆hns strain was significantly higher than that of the wild-type. Similar, albeit not identical, there were consequences when hns was deleted from the hypervirulent A. baumannii AB5075 strain. In the AB5075 complemented strain, the reduction in gene expression in a few cases was not so pronounced that it reached wild-type levels, and the expression of comEA was enhanced further. In conclusion, the expression of all seven transformation-related genes was enhanced after deleting hns in A. baumannii A118 and AB5075, and these modifications were accompanied by an increase in the cells’ transformability. The results highlight a role of H-NS in A. baumannii’s natural competence.
Fil: Le, Casin. California State University; Estados Unidos
Fil: Pimentel, Camila. California State University; Estados Unidos
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Escalante, Jenny. California State University; Estados Unidos
Fil: Nishimura, Brent. California State University; Estados Unidos
Fil: Arriaga, Susana. California State University; Estados Unidos
Fil: Rodgers, Deja. California State University; Estados Unidos
Fil: Bonomo, Robert A.. Center for Antimicrobial Resistance and Epidemiology; Estados Unidos. Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Tolmasky, Marcelo E.. California State University; Estados Unidos
Fil: Ramírez, María Soledad. California State University; Estados Unidos
description Most Acinetobacter baumannii strains are naturally competent. Although some information is available about factors that enhance or reduce the frequency of the transformation of this bacterium, the regulatory elements and mechanisms are barely understood. In this article, we describe studies on the role of the histone-like nucleoid structuring protein, H-NS, in the regulation of the expression of genes related to natural competency and the ability to uptake foreign DNA. The expression levels of the natural transformation-related genes pilA, pilT, pilQ, comEA, comEC, comF, and drpA significantly increased in a ∆hns derivative of A. baumannii A118. The complementation of the mutant with a recombinant plasmid harboring hns restored the expression levels of six of these genes (pilT remained expressed at high levels) to those of the wild-type strain. The transformation frequency of the A. baumannii A118 ∆hns strain was significantly higher than that of the wild-type. Similar, albeit not identical, there were consequences when hns was deleted from the hypervirulent A. baumannii AB5075 strain. In the AB5075 complemented strain, the reduction in gene expression in a few cases was not so pronounced that it reached wild-type levels, and the expression of comEA was enhanced further. In conclusion, the expression of all seven transformation-related genes was enhanced after deleting hns in A. baumannii A118 and AB5075, and these modifications were accompanied by an increase in the cells’ transformability. The results highlight a role of H-NS in A. baumannii’s natural competence.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212025
Le, Casin; Pimentel, Camila; Tuttobene, Marisel Romina; Subils, Tomás; Escalante, Jenny; et al.; Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation; Multidisciplinary Digital Publishing Institute; Pathogens; 10; 9; 9-2021; 1-7
2076-0817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212025
identifier_str_mv Le, Casin; Pimentel, Camila; Tuttobene, Marisel Romina; Subils, Tomás; Escalante, Jenny; et al.; Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation; Multidisciplinary Digital Publishing Institute; Pathogens; 10; 9; 9-2021; 1-7
2076-0817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/10/9/1083
info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens10091083
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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