Human fluids alter DNA-acquisition in Acinetobacter baumannii

Autores
Martinez, Jasmine; Liu, Christine; Rodman, Nyah; Fernandez, Jennifer S.; Barberis, Claudia; Sieira, Rodrigo; Perez, Federico; Bonomo, Robert A.; Ramirez, Maria Soledad
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Transformation is one of the mechanisms of acquisition of foreign genetic material leading to the emergence of multidrug resistant (MDR) bacteria. Recently, human serum albumin (HSA) was shown to specifically increase transformation frequency in the nosocomial pathogen Acinetobacter baumannii. To further assess the relevance of HSA as a possible modulator of A. baumannii transformation in host-pathogen interactions, in this work we examined the effect of different human fluids. We observed a significant increase in transformation frequencies in the presence of pleural fluid, whole blood cells and liquid ascites, and to a lesser extent with urine. The observed effects correlate with both HSA and bacterial content found in the assayed patient fluids. Taken together, these results are in agreement with our previous findings that highlight HSA as a possible host signal with the ability to trigger natural transformation in A. baumannii.
Fil: Martinez, Jasmine. California State University; Estados Unidos
Fil: Liu, Christine. California State University; Estados Unidos
Fil: Rodman, Nyah. California State University; Estados Unidos
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Barberis, Claudia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
Materia
ACINETOBACTER BAUMANNII
ANTIBIOTIC RESISTANCE
DNA-ACQUISITION
HUMAN SERUM ALBUMIN
PLEURAL FLUID
TRANSFORMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87858
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Human fluids alter DNA-acquisition in Acinetobacter baumanniiMartinez, JasmineLiu, ChristineRodman, NyahFernandez, Jennifer S.Barberis, ClaudiaSieira, RodrigoPerez, FedericoBonomo, Robert A.Ramirez, Maria SoledadACINETOBACTER BAUMANNIIANTIBIOTIC RESISTANCEDNA-ACQUISITIONHUMAN SERUM ALBUMINPLEURAL FLUIDTRANSFORMATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Transformation is one of the mechanisms of acquisition of foreign genetic material leading to the emergence of multidrug resistant (MDR) bacteria. Recently, human serum albumin (HSA) was shown to specifically increase transformation frequency in the nosocomial pathogen Acinetobacter baumannii. To further assess the relevance of HSA as a possible modulator of A. baumannii transformation in host-pathogen interactions, in this work we examined the effect of different human fluids. We observed a significant increase in transformation frequencies in the presence of pleural fluid, whole blood cells and liquid ascites, and to a lesser extent with urine. The observed effects correlate with both HSA and bacterial content found in the assayed patient fluids. Taken together, these results are in agreement with our previous findings that highlight HSA as a possible host signal with the ability to trigger natural transformation in A. baumannii.Fil: Martinez, Jasmine. California State University; Estados UnidosFil: Liu, Christine. California State University; Estados UnidosFil: Rodman, Nyah. California State University; Estados UnidosFil: Fernandez, Jennifer S.. California State University; Estados UnidosFil: Barberis, Claudia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados UnidosFil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados UnidosFil: Ramirez, Maria Soledad. California State University; Estados UnidosElsevier Science Inc2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87858Martinez, Jasmine; Liu, Christine; Rodman, Nyah; Fernandez, Jennifer S.; Barberis, Claudia; et al.; Human fluids alter DNA-acquisition in Acinetobacter baumannii; Elsevier Science Inc; Diagnostic Microbiology and Infectious Diseases; 93; 3; 3-2019; 183-1870732-8893CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.diagmicrobio.2018.10.010info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0732889318304899info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:22Zoai:ri.conicet.gov.ar:11336/87858instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:22.287CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human fluids alter DNA-acquisition in Acinetobacter baumannii
title Human fluids alter DNA-acquisition in Acinetobacter baumannii
spellingShingle Human fluids alter DNA-acquisition in Acinetobacter baumannii
Martinez, Jasmine
ACINETOBACTER BAUMANNII
ANTIBIOTIC RESISTANCE
DNA-ACQUISITION
HUMAN SERUM ALBUMIN
PLEURAL FLUID
TRANSFORMATION
title_short Human fluids alter DNA-acquisition in Acinetobacter baumannii
title_full Human fluids alter DNA-acquisition in Acinetobacter baumannii
title_fullStr Human fluids alter DNA-acquisition in Acinetobacter baumannii
title_full_unstemmed Human fluids alter DNA-acquisition in Acinetobacter baumannii
title_sort Human fluids alter DNA-acquisition in Acinetobacter baumannii
dc.creator.none.fl_str_mv Martinez, Jasmine
Liu, Christine
Rodman, Nyah
Fernandez, Jennifer S.
Barberis, Claudia
Sieira, Rodrigo
Perez, Federico
Bonomo, Robert A.
Ramirez, Maria Soledad
author Martinez, Jasmine
author_facet Martinez, Jasmine
Liu, Christine
Rodman, Nyah
Fernandez, Jennifer S.
Barberis, Claudia
Sieira, Rodrigo
Perez, Federico
Bonomo, Robert A.
Ramirez, Maria Soledad
author_role author
author2 Liu, Christine
Rodman, Nyah
Fernandez, Jennifer S.
Barberis, Claudia
Sieira, Rodrigo
Perez, Federico
Bonomo, Robert A.
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER BAUMANNII
ANTIBIOTIC RESISTANCE
DNA-ACQUISITION
HUMAN SERUM ALBUMIN
PLEURAL FLUID
TRANSFORMATION
topic ACINETOBACTER BAUMANNII
ANTIBIOTIC RESISTANCE
DNA-ACQUISITION
HUMAN SERUM ALBUMIN
PLEURAL FLUID
TRANSFORMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Transformation is one of the mechanisms of acquisition of foreign genetic material leading to the emergence of multidrug resistant (MDR) bacteria. Recently, human serum albumin (HSA) was shown to specifically increase transformation frequency in the nosocomial pathogen Acinetobacter baumannii. To further assess the relevance of HSA as a possible modulator of A. baumannii transformation in host-pathogen interactions, in this work we examined the effect of different human fluids. We observed a significant increase in transformation frequencies in the presence of pleural fluid, whole blood cells and liquid ascites, and to a lesser extent with urine. The observed effects correlate with both HSA and bacterial content found in the assayed patient fluids. Taken together, these results are in agreement with our previous findings that highlight HSA as a possible host signal with the ability to trigger natural transformation in A. baumannii.
Fil: Martinez, Jasmine. California State University; Estados Unidos
Fil: Liu, Christine. California State University; Estados Unidos
Fil: Rodman, Nyah. California State University; Estados Unidos
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Barberis, Claudia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados Unidos
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
description Transformation is one of the mechanisms of acquisition of foreign genetic material leading to the emergence of multidrug resistant (MDR) bacteria. Recently, human serum albumin (HSA) was shown to specifically increase transformation frequency in the nosocomial pathogen Acinetobacter baumannii. To further assess the relevance of HSA as a possible modulator of A. baumannii transformation in host-pathogen interactions, in this work we examined the effect of different human fluids. We observed a significant increase in transformation frequencies in the presence of pleural fluid, whole blood cells and liquid ascites, and to a lesser extent with urine. The observed effects correlate with both HSA and bacterial content found in the assayed patient fluids. Taken together, these results are in agreement with our previous findings that highlight HSA as a possible host signal with the ability to trigger natural transformation in A. baumannii.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87858
Martinez, Jasmine; Liu, Christine; Rodman, Nyah; Fernandez, Jennifer S.; Barberis, Claudia; et al.; Human fluids alter DNA-acquisition in Acinetobacter baumannii; Elsevier Science Inc; Diagnostic Microbiology and Infectious Diseases; 93; 3; 3-2019; 183-187
0732-8893
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87858
identifier_str_mv Martinez, Jasmine; Liu, Christine; Rodman, Nyah; Fernandez, Jennifer S.; Barberis, Claudia; et al.; Human fluids alter DNA-acquisition in Acinetobacter baumannii; Elsevier Science Inc; Diagnostic Microbiology and Infectious Diseases; 93; 3; 3-2019; 183-187
0732-8893
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diagmicrobio.2018.10.010
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0732889318304899
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270000502538240
score 13.13397

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