Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set

Autores
Rogberg Muñoz, Andres; Texeira, L. Viana; Villegas Castagnasso, Egle Etel; Teixeira, C. Salviano; Peral Garcia, Pilar; Oliveira, D. A. Andrade De; Giovambattista, Guillermo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Buffaloes (Bubalus bubalis) were introduced to Brazil in 1895. As cross-species utility of microsatellites is one of the preferred marker choice criteria, 19 cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three panels were finally developed with eleven that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. HWE tests results shows a disequilibrium (P < 0.05) in nine of the eleven loci, due to a significant increase of homozygotes in 8 of them. As it was expected, Brazilian Buffalo shows lower variability than Buffaloes from the domestication centres (China and India) but higher than the seriously selected European populations. Calculated overall exclusion power of the set in Brazilian buffalo allows its use in DNA based traceability. Furthermore, five highly informative tested microsatellites could be added to existing panels.
Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Texeira, L. Viana. Universidade Federal de Minas Gerais; Brasil
Fil: Villegas Castagnasso, Egle Etel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Teixeira, C. Salviano. Universidade Federal de Minas Gerais; Brasil
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Oliveira, D. A. Andrade De. Universidade Federal de Minas Gerais; Brasil
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
BUFFALO
MICROSATELLITE
VARIABILITY
DNA MARKERS
TRACEABILITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79628
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite SetRogberg Muñoz, AndresTexeira, L. VianaVillegas Castagnasso, Egle EtelTeixeira, C. SalvianoPeral Garcia, PilarOliveira, D. A. Andrade DeGiovambattista, GuillermoBUFFALOMICROSATELLITEVARIABILITYDNA MARKERSTRACEABILITYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Buffaloes (Bubalus bubalis) were introduced to Brazil in 1895. As cross-species utility of microsatellites is one of the preferred marker choice criteria, 19 cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three panels were finally developed with eleven that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. HWE tests results shows a disequilibrium (P < 0.05) in nine of the eleven loci, due to a significant increase of homozygotes in 8 of them. As it was expected, Brazilian Buffalo shows lower variability than Buffaloes from the domestication centres (China and India) but higher than the seriously selected European populations. Calculated overall exclusion power of the set in Brazilian buffalo allows its use in DNA based traceability. Furthermore, five highly informative tested microsatellites could be added to existing panels.Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Texeira, L. Viana. Universidade Federal de Minas Gerais; BrasilFil: Villegas Castagnasso, Egle Etel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Teixeira, C. Salviano. Universidade Federal de Minas Gerais; BrasilFil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Oliveira, D. A. Andrade De. Universidade Federal de Minas Gerais; BrasilFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaDavid Publishing2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79628Rogberg Muñoz, Andres; Texeira, L. Viana; Villegas Castagnasso, Egle Etel; Teixeira, C. Salviano; Peral Garcia, Pilar; et al.; Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set; David Publishing; Journal of Agricultural Science and Technology B; B1; 11-2011; 1008-10122161-6264CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.davidpublisher.org/index.php/Home/Article/index?id=15917.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.17265/2161-6264/2011.11B.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:28Zoai:ri.conicet.gov.ar:11336/79628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:28.901CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
spellingShingle Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
Rogberg Muñoz, Andres
BUFFALO
MICROSATELLITE
VARIABILITY
DNA MARKERS
TRACEABILITY
title_short Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_full Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_fullStr Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_full_unstemmed Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
title_sort Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set
dc.creator.none.fl_str_mv Rogberg Muñoz, Andres
Texeira, L. Viana
Villegas Castagnasso, Egle Etel
Teixeira, C. Salviano
Peral Garcia, Pilar
Oliveira, D. A. Andrade De
Giovambattista, Guillermo
author Rogberg Muñoz, Andres
author_facet Rogberg Muñoz, Andres
Texeira, L. Viana
Villegas Castagnasso, Egle Etel
Teixeira, C. Salviano
Peral Garcia, Pilar
Oliveira, D. A. Andrade De
Giovambattista, Guillermo
author_role author
author2 Texeira, L. Viana
Villegas Castagnasso, Egle Etel
Teixeira, C. Salviano
Peral Garcia, Pilar
Oliveira, D. A. Andrade De
Giovambattista, Guillermo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BUFFALO
MICROSATELLITE
VARIABILITY
DNA MARKERS
TRACEABILITY
topic BUFFALO
MICROSATELLITE
VARIABILITY
DNA MARKERS
TRACEABILITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Buffaloes (Bubalus bubalis) were introduced to Brazil in 1895. As cross-species utility of microsatellites is one of the preferred marker choice criteria, 19 cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three panels were finally developed with eleven that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. HWE tests results shows a disequilibrium (P < 0.05) in nine of the eleven loci, due to a significant increase of homozygotes in 8 of them. As it was expected, Brazilian Buffalo shows lower variability than Buffaloes from the domestication centres (China and India) but higher than the seriously selected European populations. Calculated overall exclusion power of the set in Brazilian buffalo allows its use in DNA based traceability. Furthermore, five highly informative tested microsatellites could be added to existing panels.
Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Texeira, L. Viana. Universidade Federal de Minas Gerais; Brasil
Fil: Villegas Castagnasso, Egle Etel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Teixeira, C. Salviano. Universidade Federal de Minas Gerais; Brasil
Fil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Oliveira, D. A. Andrade De. Universidade Federal de Minas Gerais; Brasil
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Buffaloes (Bubalus bubalis) were introduced to Brazil in 1895. As cross-species utility of microsatellites is one of the preferred marker choice criteria, 19 cattle microsatellites were tested to develop a multiplexed set of microsatellites and characterize Brazilian buffalo. Three panels were finally developed with eleven that succeed in amplify and were polymorphic (58%). The average number of alleles was 5.42, with an average observed and expected heterocigozity of 0.441 and 0.695, respectively. HWE tests results shows a disequilibrium (P < 0.05) in nine of the eleven loci, due to a significant increase of homozygotes in 8 of them. As it was expected, Brazilian Buffalo shows lower variability than Buffaloes from the domestication centres (China and India) but higher than the seriously selected European populations. Calculated overall exclusion power of the set in Brazilian buffalo allows its use in DNA based traceability. Furthermore, five highly informative tested microsatellites could be added to existing panels.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79628
Rogberg Muñoz, Andres; Texeira, L. Viana; Villegas Castagnasso, Egle Etel; Teixeira, C. Salviano; Peral Garcia, Pilar; et al.; Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set; David Publishing; Journal of Agricultural Science and Technology B; B1; 11-2011; 1008-1012
2161-6264
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79628
identifier_str_mv Rogberg Muñoz, Andres; Texeira, L. Viana; Villegas Castagnasso, Egle Etel; Teixeira, C. Salviano; Peral Garcia, Pilar; et al.; Brazilian Buffalo Genetic Variability by Cross-Specific Microsatellite Set; David Publishing; Journal of Agricultural Science and Technology B; B1; 11-2011; 1008-1012
2161-6264
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.davidpublisher.org/index.php/Home/Article/index?id=15917.html
info:eu-repo/semantics/altIdentifier/doi/10.17265/2161-6264/2011.11B.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv David Publishing
publisher.none.fl_str_mv David Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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