Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina

Autores
Wassermann, Eliana; Montecchia, Marcela Susana; Correa, Olga Susana; Vega, Damián; Romero, Ana María
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tomato bacterial wilt and canker, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is considered one of the most important bacterial diseases of tomato worldwide. During the last two decades, severe outbreaks have occurred in greenhouses in the horticultural belt of Buenos Aires-La Plata, Argentina. Cmm strains collected in this area over a period of 14 years (2000–2013) were characterized for genetic diversity by rep-PCR genomic fingerprinting and level of virulence in order to have a better understanding of the source of inoculum and virulence variability. Analyses of BOX-, ERIC- and REP-PCR fingerprints revealed that the strains were genetically diverse; the same three fingerprint types were obtained in all three cases. No relationship could be established between rep-PCR clustering and the year, location or greenhouse origin of isolates, which suggests different sources of inoculum. However, in a few cases, bacteria with identical fingerprint types were isolated from the same greenhouse in different years. Despite strains differing in virulence, particularly within BOX-PCR groups, putative virulence genes located in plasmids (celA, pat-1) or in a pathogenicity island in the chromosome (tomA, chpC, chpG and ppaA) were detected in all strains. Our results suggest that new strains introduced every year via seed importation might be coexisting with others persisting locally. This study highlights the importance of preventive measures to manage tomato bacterial wilt and canker.
Fil: Wassermann, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
Fil: Montecchia, Marcela Susana. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Correa, Olga Susana. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Vega, Damián. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Romero, Ana María. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Materia
Epidemiology
Rep-Pcr
Tomato Bacterial Wilt And Canker
Virulence Genes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55784
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in ArgentinaWassermann, ElianaMontecchia, Marcela SusanaCorrea, Olga SusanaVega, DamiánRomero, Ana MaríaEpidemiologyRep-PcrTomato Bacterial Wilt And CankerVirulence GenesTomato bacterial wilt and canker, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is considered one of the most important bacterial diseases of tomato worldwide. During the last two decades, severe outbreaks have occurred in greenhouses in the horticultural belt of Buenos Aires-La Plata, Argentina. Cmm strains collected in this area over a period of 14 years (2000–2013) were characterized for genetic diversity by rep-PCR genomic fingerprinting and level of virulence in order to have a better understanding of the source of inoculum and virulence variability. Analyses of BOX-, ERIC- and REP-PCR fingerprints revealed that the strains were genetically diverse; the same three fingerprint types were obtained in all three cases. No relationship could be established between rep-PCR clustering and the year, location or greenhouse origin of isolates, which suggests different sources of inoculum. However, in a few cases, bacteria with identical fingerprint types were isolated from the same greenhouse in different years. Despite strains differing in virulence, particularly within BOX-PCR groups, putative virulence genes located in plasmids (celA, pat-1) or in a pathogenicity island in the chromosome (tomA, chpC, chpG and ppaA) were detected in all strains. Our results suggest that new strains introduced every year via seed importation might be coexisting with others persisting locally. This study highlights the importance of preventive measures to manage tomato bacterial wilt and canker.Fil: Wassermann, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; ArgentinaFil: Montecchia, Marcela Susana. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Correa, Olga Susana. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Vega, Damián. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; ArgentinaFil: Romero, Ana María. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; ArgentinaSpringer2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55784Wassermann, Eliana; Montecchia, Marcela Susana; Correa, Olga Susana; Vega, Damián; Romero, Ana María; Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina; Springer; European Journal of Plant Pathology; 149; 1; 9-2017; 35-420929-18731573-8469CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-017-1159-zinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10658-017-1159-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:07Zoai:ri.conicet.gov.ar:11336/55784instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:08.199CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
title Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
spellingShingle Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
Wassermann, Eliana
Epidemiology
Rep-Pcr
Tomato Bacterial Wilt And Canker
Virulence Genes
title_short Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
title_full Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
title_fullStr Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
title_full_unstemmed Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
title_sort Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina
dc.creator.none.fl_str_mv Wassermann, Eliana
Montecchia, Marcela Susana
Correa, Olga Susana
Vega, Damián
Romero, Ana María
author Wassermann, Eliana
author_facet Wassermann, Eliana
Montecchia, Marcela Susana
Correa, Olga Susana
Vega, Damián
Romero, Ana María
author_role author
author2 Montecchia, Marcela Susana
Correa, Olga Susana
Vega, Damián
Romero, Ana María
author2_role author
author
author
author
dc.subject.none.fl_str_mv Epidemiology
Rep-Pcr
Tomato Bacterial Wilt And Canker
Virulence Genes
topic Epidemiology
Rep-Pcr
Tomato Bacterial Wilt And Canker
Virulence Genes
dc.description.none.fl_txt_mv Tomato bacterial wilt and canker, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is considered one of the most important bacterial diseases of tomato worldwide. During the last two decades, severe outbreaks have occurred in greenhouses in the horticultural belt of Buenos Aires-La Plata, Argentina. Cmm strains collected in this area over a period of 14 years (2000–2013) were characterized for genetic diversity by rep-PCR genomic fingerprinting and level of virulence in order to have a better understanding of the source of inoculum and virulence variability. Analyses of BOX-, ERIC- and REP-PCR fingerprints revealed that the strains were genetically diverse; the same three fingerprint types were obtained in all three cases. No relationship could be established between rep-PCR clustering and the year, location or greenhouse origin of isolates, which suggests different sources of inoculum. However, in a few cases, bacteria with identical fingerprint types were isolated from the same greenhouse in different years. Despite strains differing in virulence, particularly within BOX-PCR groups, putative virulence genes located in plasmids (celA, pat-1) or in a pathogenicity island in the chromosome (tomA, chpC, chpG and ppaA) were detected in all strains. Our results suggest that new strains introduced every year via seed importation might be coexisting with others persisting locally. This study highlights the importance of preventive measures to manage tomato bacterial wilt and canker.
Fil: Wassermann, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
Fil: Montecchia, Marcela Susana. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Correa, Olga Susana. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Vega, Damián. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Romero, Ana María. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
description Tomato bacterial wilt and canker, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is considered one of the most important bacterial diseases of tomato worldwide. During the last two decades, severe outbreaks have occurred in greenhouses in the horticultural belt of Buenos Aires-La Plata, Argentina. Cmm strains collected in this area over a period of 14 years (2000–2013) were characterized for genetic diversity by rep-PCR genomic fingerprinting and level of virulence in order to have a better understanding of the source of inoculum and virulence variability. Analyses of BOX-, ERIC- and REP-PCR fingerprints revealed that the strains were genetically diverse; the same three fingerprint types were obtained in all three cases. No relationship could be established between rep-PCR clustering and the year, location or greenhouse origin of isolates, which suggests different sources of inoculum. However, in a few cases, bacteria with identical fingerprint types were isolated from the same greenhouse in different years. Despite strains differing in virulence, particularly within BOX-PCR groups, putative virulence genes located in plasmids (celA, pat-1) or in a pathogenicity island in the chromosome (tomA, chpC, chpG and ppaA) were detected in all strains. Our results suggest that new strains introduced every year via seed importation might be coexisting with others persisting locally. This study highlights the importance of preventive measures to manage tomato bacterial wilt and canker.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55784
Wassermann, Eliana; Montecchia, Marcela Susana; Correa, Olga Susana; Vega, Damián; Romero, Ana María; Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina; Springer; European Journal of Plant Pathology; 149; 1; 9-2017; 35-42
0929-1873
1573-8469
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55784
identifier_str_mv Wassermann, Eliana; Montecchia, Marcela Susana; Correa, Olga Susana; Vega, Damián; Romero, Ana María; Clavibacter michiganensis subsp. michiganensis strains virulence and genetic diversity. a first study in Argentina; Springer; European Journal of Plant Pathology; 149; 1; 9-2017; 35-42
0929-1873
1573-8469
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-017-1159-z
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10658-017-1159-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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