A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis
- Autores
- Yañez Olvera, Alan Guillermo; Gómez Díaz, Ambar Grissel; Sélem Mojica, Nelly; Rodríguez Orduña, Lorena; Lara Ávila, José Pablo; Varni, Vanina Delia; Alcoba, Florencia; Croce, Valentina; Legros, Thierry; Torres, Alberto; Torres Ruíz, Alfonso; Tarrats, Félix; Vermunt, Adriaan; Looije, Thorben; Cibrian Jaramillo, Angélica; Valenzuela, Miryam; Siri, María Inés; Barona Gomez, Francisco
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Actinomycetota (formerly Actinobacteria) genus Clavibacter includes phytopathogens with devasting effects in several crops. Clavibacter michiganensis, the causal agent of tomato bacterial canker, is the most notorious species of the genus. Yet, its origin and natural reservoirs remain elusive, and its populations show pathogenicity profiles with unpredictable plant disease outcomes. Here, we generate and analyse a decade-long genomic dataset of Clavibacter from wild and commercial tomato cultivars, providing evolutionary insights that directed phenotypic characterization. Our phylogeny situates the last common ancestor of C. michiganensis next to Clavibacter isolates from grasses rather than to the sole strain we could isolate from wild tomatoes. Pathogenicity profiling of C. michiganensis isolates, together with C. phaseoli and C. californiensis as sister taxa and the wild tomato strain, was found to be congruent with the proposed phylogenetic relationships. We then identified gene enrichment after the evolutionary event, leading to the appearance of the C. michiganesis clade, including known pathogenicity factors but also hitherto unnoticed genes with the ability to encode adaptive traits for a pathogenic lifestyle. The holistic perspective provided by our evolutionary analyses hints towards a host shift event as the origin of C. michiganensis as a tomato pathogen and the existence of pathogenic genes that remain to be characterized.
Fil: Yañez Olvera, Alan Guillermo. Leiden University. Institute Biology Leiden; Países Bajos. Instituto Politécnico Nacional; México
Fil: Gómez Díaz, Ambar Grissel. Instituto Politécnico Nacional; México
Fil: Sélem Mojica, Nelly. Instituto Politécnico Nacional; México
Fil: Rodríguez Orduña, Lorena. Instituto Politécnico Nacional; México
Fil: Lara Ávila, José Pablo. Instituto Politécnico Nacional; México
Fil: Varni, Vanina Delia. Instituto Politécnico Nacional; México. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alcoba, Florencia. Universidad de la República; Uruguay
Fil: Croce, Valentina. Universidad de la República; Uruguay
Fil: Legros, Thierry. Red Sun Farms; México
Fil: Torres, Alberto. Red Sun Farms; México
Fil: Torres Ruíz, Alfonso. Koppert; México
Fil: Tarrats, Félix. Centro De Estudios Universitarios; Argentina
Fil: Vermunt, Adriaan. Normec Groen Agro Control; Países Bajos
Fil: Looije, Thorben. Valto B.V; Países Bajos
Fil: Cibrian Jaramillo, Angélica. Naturalis Biodiversity Center; Países Bajos
Fil: Valenzuela, Miryam. Universidad Técnica Federico Santa María; Chile
Fil: Siri, María Inés. Universidad de la República; Uruguay
Fil: Barona Gomez, Francisco. Leiden University. Institute Biology Leiden; Países Bajos. Instituto Politécnico Nacional; México - Materia
-
bacterial canker
Clavibacter michiganensis
host shift
pangenomics - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/258192
Ver los metadatos del registro completo
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A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensisYañez Olvera, Alan GuillermoGómez Díaz, Ambar GrisselSélem Mojica, NellyRodríguez Orduña, LorenaLara Ávila, José PabloVarni, Vanina DeliaAlcoba, FlorenciaCroce, ValentinaLegros, ThierryTorres, AlbertoTorres Ruíz, AlfonsoTarrats, FélixVermunt, AdriaanLooije, ThorbenCibrian Jaramillo, AngélicaValenzuela, MiryamSiri, María InésBarona Gomez, Franciscobacterial cankerClavibacter michiganensishost shiftpangenomicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Actinomycetota (formerly Actinobacteria) genus Clavibacter includes phytopathogens with devasting effects in several crops. Clavibacter michiganensis, the causal agent of tomato bacterial canker, is the most notorious species of the genus. Yet, its origin and natural reservoirs remain elusive, and its populations show pathogenicity profiles with unpredictable plant disease outcomes. Here, we generate and analyse a decade-long genomic dataset of Clavibacter from wild and commercial tomato cultivars, providing evolutionary insights that directed phenotypic characterization. Our phylogeny situates the last common ancestor of C. michiganensis next to Clavibacter isolates from grasses rather than to the sole strain we could isolate from wild tomatoes. Pathogenicity profiling of C. michiganensis isolates, together with C. phaseoli and C. californiensis as sister taxa and the wild tomato strain, was found to be congruent with the proposed phylogenetic relationships. We then identified gene enrichment after the evolutionary event, leading to the appearance of the C. michiganesis clade, including known pathogenicity factors but also hitherto unnoticed genes with the ability to encode adaptive traits for a pathogenic lifestyle. The holistic perspective provided by our evolutionary analyses hints towards a host shift event as the origin of C. michiganensis as a tomato pathogen and the existence of pathogenic genes that remain to be characterized.Fil: Yañez Olvera, Alan Guillermo. Leiden University. Institute Biology Leiden; Países Bajos. Instituto Politécnico Nacional; MéxicoFil: Gómez Díaz, Ambar Grissel. Instituto Politécnico Nacional; MéxicoFil: Sélem Mojica, Nelly. Instituto Politécnico Nacional; MéxicoFil: Rodríguez Orduña, Lorena. Instituto Politécnico Nacional; MéxicoFil: Lara Ávila, José Pablo. Instituto Politécnico Nacional; MéxicoFil: Varni, Vanina Delia. Instituto Politécnico Nacional; México. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alcoba, Florencia. Universidad de la República; UruguayFil: Croce, Valentina. Universidad de la República; UruguayFil: Legros, Thierry. Red Sun Farms; MéxicoFil: Torres, Alberto. Red Sun Farms; MéxicoFil: Torres Ruíz, Alfonso. Koppert; MéxicoFil: Tarrats, Félix. Centro De Estudios Universitarios; ArgentinaFil: Vermunt, Adriaan. Normec Groen Agro Control; Países BajosFil: Looije, Thorben. Valto B.V; Países BajosFil: Cibrian Jaramillo, Angélica. Naturalis Biodiversity Center; Países BajosFil: Valenzuela, Miryam. Universidad Técnica Federico Santa María; ChileFil: Siri, María Inés. Universidad de la República; UruguayFil: Barona Gomez, Francisco. Leiden University. Institute Biology Leiden; Países Bajos. Instituto Politécnico Nacional; MéxicoMicrobiology Society2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258192Yañez Olvera, Alan Guillermo; Gómez Díaz, Ambar Grissel; Sélem Mojica, Nelly; Rodríguez Orduña, Lorena; Lara Ávila, José Pablo; et al.; A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis; Microbiology Society; Microbial Genomics; 10; 10; 10-2024; 1-182057-5858CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.001309info:eu-repo/semantics/altIdentifier/doi/10.1099/mgen.0.001309info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:00Zoai:ri.conicet.gov.ar:11336/258192instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:01.144CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis |
| title |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis |
| spellingShingle |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis Yañez Olvera, Alan Guillermo bacterial canker Clavibacter michiganensis host shift pangenomics |
| title_short |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis |
| title_full |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis |
| title_fullStr |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis |
| title_full_unstemmed |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis |
| title_sort |
A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis |
| dc.creator.none.fl_str_mv |
Yañez Olvera, Alan Guillermo Gómez Díaz, Ambar Grissel Sélem Mojica, Nelly Rodríguez Orduña, Lorena Lara Ávila, José Pablo Varni, Vanina Delia Alcoba, Florencia Croce, Valentina Legros, Thierry Torres, Alberto Torres Ruíz, Alfonso Tarrats, Félix Vermunt, Adriaan Looije, Thorben Cibrian Jaramillo, Angélica Valenzuela, Miryam Siri, María Inés Barona Gomez, Francisco |
| author |
Yañez Olvera, Alan Guillermo |
| author_facet |
Yañez Olvera, Alan Guillermo Gómez Díaz, Ambar Grissel Sélem Mojica, Nelly Rodríguez Orduña, Lorena Lara Ávila, José Pablo Varni, Vanina Delia Alcoba, Florencia Croce, Valentina Legros, Thierry Torres, Alberto Torres Ruíz, Alfonso Tarrats, Félix Vermunt, Adriaan Looije, Thorben Cibrian Jaramillo, Angélica Valenzuela, Miryam Siri, María Inés Barona Gomez, Francisco |
| author_role |
author |
| author2 |
Gómez Díaz, Ambar Grissel Sélem Mojica, Nelly Rodríguez Orduña, Lorena Lara Ávila, José Pablo Varni, Vanina Delia Alcoba, Florencia Croce, Valentina Legros, Thierry Torres, Alberto Torres Ruíz, Alfonso Tarrats, Félix Vermunt, Adriaan Looije, Thorben Cibrian Jaramillo, Angélica Valenzuela, Miryam Siri, María Inés Barona Gomez, Francisco |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
bacterial canker Clavibacter michiganensis host shift pangenomics |
| topic |
bacterial canker Clavibacter michiganensis host shift pangenomics |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The Actinomycetota (formerly Actinobacteria) genus Clavibacter includes phytopathogens with devasting effects in several crops. Clavibacter michiganensis, the causal agent of tomato bacterial canker, is the most notorious species of the genus. Yet, its origin and natural reservoirs remain elusive, and its populations show pathogenicity profiles with unpredictable plant disease outcomes. Here, we generate and analyse a decade-long genomic dataset of Clavibacter from wild and commercial tomato cultivars, providing evolutionary insights that directed phenotypic characterization. Our phylogeny situates the last common ancestor of C. michiganensis next to Clavibacter isolates from grasses rather than to the sole strain we could isolate from wild tomatoes. Pathogenicity profiling of C. michiganensis isolates, together with C. phaseoli and C. californiensis as sister taxa and the wild tomato strain, was found to be congruent with the proposed phylogenetic relationships. We then identified gene enrichment after the evolutionary event, leading to the appearance of the C. michiganesis clade, including known pathogenicity factors but also hitherto unnoticed genes with the ability to encode adaptive traits for a pathogenic lifestyle. The holistic perspective provided by our evolutionary analyses hints towards a host shift event as the origin of C. michiganensis as a tomato pathogen and the existence of pathogenic genes that remain to be characterized. Fil: Yañez Olvera, Alan Guillermo. Leiden University. Institute Biology Leiden; Países Bajos. Instituto Politécnico Nacional; México Fil: Gómez Díaz, Ambar Grissel. Instituto Politécnico Nacional; México Fil: Sélem Mojica, Nelly. Instituto Politécnico Nacional; México Fil: Rodríguez Orduña, Lorena. Instituto Politécnico Nacional; México Fil: Lara Ávila, José Pablo. Instituto Politécnico Nacional; México Fil: Varni, Vanina Delia. Instituto Politécnico Nacional; México. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alcoba, Florencia. Universidad de la República; Uruguay Fil: Croce, Valentina. Universidad de la República; Uruguay Fil: Legros, Thierry. Red Sun Farms; México Fil: Torres, Alberto. Red Sun Farms; México Fil: Torres Ruíz, Alfonso. Koppert; México Fil: Tarrats, Félix. Centro De Estudios Universitarios; Argentina Fil: Vermunt, Adriaan. Normec Groen Agro Control; Países Bajos Fil: Looije, Thorben. Valto B.V; Países Bajos Fil: Cibrian Jaramillo, Angélica. Naturalis Biodiversity Center; Países Bajos Fil: Valenzuela, Miryam. Universidad Técnica Federico Santa María; Chile Fil: Siri, María Inés. Universidad de la República; Uruguay Fil: Barona Gomez, Francisco. Leiden University. Institute Biology Leiden; Países Bajos. Instituto Politécnico Nacional; México |
| description |
The Actinomycetota (formerly Actinobacteria) genus Clavibacter includes phytopathogens with devasting effects in several crops. Clavibacter michiganensis, the causal agent of tomato bacterial canker, is the most notorious species of the genus. Yet, its origin and natural reservoirs remain elusive, and its populations show pathogenicity profiles with unpredictable plant disease outcomes. Here, we generate and analyse a decade-long genomic dataset of Clavibacter from wild and commercial tomato cultivars, providing evolutionary insights that directed phenotypic characterization. Our phylogeny situates the last common ancestor of C. michiganensis next to Clavibacter isolates from grasses rather than to the sole strain we could isolate from wild tomatoes. Pathogenicity profiling of C. michiganensis isolates, together with C. phaseoli and C. californiensis as sister taxa and the wild tomato strain, was found to be congruent with the proposed phylogenetic relationships. We then identified gene enrichment after the evolutionary event, leading to the appearance of the C. michiganesis clade, including known pathogenicity factors but also hitherto unnoticed genes with the ability to encode adaptive traits for a pathogenic lifestyle. The holistic perspective provided by our evolutionary analyses hints towards a host shift event as the origin of C. michiganensis as a tomato pathogen and the existence of pathogenic genes that remain to be characterized. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-10 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/258192 Yañez Olvera, Alan Guillermo; Gómez Díaz, Ambar Grissel; Sélem Mojica, Nelly; Rodríguez Orduña, Lorena; Lara Ávila, José Pablo; et al.; A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis; Microbiology Society; Microbial Genomics; 10; 10; 10-2024; 1-18 2057-5858 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/258192 |
| identifier_str_mv |
Yañez Olvera, Alan Guillermo; Gómez Díaz, Ambar Grissel; Sélem Mojica, Nelly; Rodríguez Orduña, Lorena; Lara Ávila, José Pablo; et al.; A host shift as the origin of tomato bacterial canker caused by Clavibacter michiganensis; Microbiology Society; Microbial Genomics; 10; 10; 10-2024; 1-18 2057-5858 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.001309 info:eu-repo/semantics/altIdentifier/doi/10.1099/mgen.0.001309 |
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openAccess |
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Microbiology Society |
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Microbiology Society |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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