Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants

Autores
Romero, Ana M.; Vega, Damián; Pizzorno, Romina; Cordon, Gabriela Beatriz; Correa, Olga S.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clavibacter michiganensis subsp. michiganensis causes bacterial wilt and canker in tomato. A detailed account of pathogen effects on growth and water parameters of affected plants is lacking. Besides, the long latent period of the disease represents a limitation for its management. Our objectives were to determine the physiological and morphological changes induced by this pathogen in tomato, in relation to water stress, and to evaluate two well-known leaf spectral reflectance indices for the diagnosis of this vascular disease before symptoms expression. Experiments were conducted in a greenhouse with pot-grown plants inoculated with a bacterial suspension (107 CFU/ml); controls were treated with sterile distilled water. Five weeks later plants were harvested and several growth parameters, the number and size of xylem vessels and the hydraulic conductivity of the stems were determined. Inoculated plants were shorter, with smaller leaf area and lower biomass than those of mock-inoculated plants. While the number and hydraulic radius of xylem vessels were not affected, the stem-specific (Ks) and the leaf-specific (KL) conductivity were significantly reduced by inoculation. Wilting of leaves of diseased plants was associated to the reduction of KL. Inoculated plants showed leaf spectral reflectance changes 1 week after inoculation, and 2 weeks before symptom expression, as shown by the index NDWI, an indicator of the water content of the leaves. We conclude that C. michiganensis subsp. michiganensis reduced the growth and altered plant-water relations of tomato plants by reducing the stem hydraulic conductivity. The NDWI index could be used for the early, non- destructive, diagnosis of bacterial canker.
Fil: Romero, Ana M.. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Vega, Damián. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Pizzorno, Romina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Cordon, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Correa, Olga S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
Materia
Hydraulic conductivity
Xylem vessels
Vascular disease
Bacterial canker and wilt
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93205
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plantsRomero, Ana M.Vega, DamiánPizzorno, RominaCordon, Gabriela BeatrizCorrea, Olga S.Hydraulic conductivityXylem vesselsVascular diseaseBacterial canker and wilthttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Clavibacter michiganensis subsp. michiganensis causes bacterial wilt and canker in tomato. A detailed account of pathogen effects on growth and water parameters of affected plants is lacking. Besides, the long latent period of the disease represents a limitation for its management. Our objectives were to determine the physiological and morphological changes induced by this pathogen in tomato, in relation to water stress, and to evaluate two well-known leaf spectral reflectance indices for the diagnosis of this vascular disease before symptoms expression. Experiments were conducted in a greenhouse with pot-grown plants inoculated with a bacterial suspension (107 CFU/ml); controls were treated with sterile distilled water. Five weeks later plants were harvested and several growth parameters, the number and size of xylem vessels and the hydraulic conductivity of the stems were determined. Inoculated plants were shorter, with smaller leaf area and lower biomass than those of mock-inoculated plants. While the number and hydraulic radius of xylem vessels were not affected, the stem-specific (Ks) and the leaf-specific (KL) conductivity were significantly reduced by inoculation. Wilting of leaves of diseased plants was associated to the reduction of KL. Inoculated plants showed leaf spectral reflectance changes 1 week after inoculation, and 2 weeks before symptom expression, as shown by the index NDWI, an indicator of the water content of the leaves. We conclude that C. michiganensis subsp. michiganensis reduced the growth and altered plant-water relations of tomato plants by reducing the stem hydraulic conductivity. The NDWI index could be used for the early, non- destructive, diagnosis of bacterial canker.Fil: Romero, Ana M.. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; ArgentinaFil: Vega, Damián. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; ArgentinaFil: Pizzorno, Romina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; ArgentinaFil: Cordon, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Correa, Olga S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; ArgentinaSpringer2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93205Romero, Ana M.; Vega, Damián; Pizzorno, Romina; Cordon, Gabriela Beatriz; Correa, Olga S.; Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants; Springer; European Journal of Plant Pathology; 152; 2; 5-2018; 567-5720929-1873CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10658-018-1500-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-018-1500-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:45Zoai:ri.conicet.gov.ar:11336/93205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:45.61CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
title Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
spellingShingle Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
Romero, Ana M.
Hydraulic conductivity
Xylem vessels
Vascular disease
Bacterial canker and wilt
title_short Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
title_full Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
title_fullStr Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
title_full_unstemmed Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
title_sort Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants
dc.creator.none.fl_str_mv Romero, Ana M.
Vega, Damián
Pizzorno, Romina
Cordon, Gabriela Beatriz
Correa, Olga S.
author Romero, Ana M.
author_facet Romero, Ana M.
Vega, Damián
Pizzorno, Romina
Cordon, Gabriela Beatriz
Correa, Olga S.
author_role author
author2 Vega, Damián
Pizzorno, Romina
Cordon, Gabriela Beatriz
Correa, Olga S.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hydraulic conductivity
Xylem vessels
Vascular disease
Bacterial canker and wilt
topic Hydraulic conductivity
Xylem vessels
Vascular disease
Bacterial canker and wilt
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Clavibacter michiganensis subsp. michiganensis causes bacterial wilt and canker in tomato. A detailed account of pathogen effects on growth and water parameters of affected plants is lacking. Besides, the long latent period of the disease represents a limitation for its management. Our objectives were to determine the physiological and morphological changes induced by this pathogen in tomato, in relation to water stress, and to evaluate two well-known leaf spectral reflectance indices for the diagnosis of this vascular disease before symptoms expression. Experiments were conducted in a greenhouse with pot-grown plants inoculated with a bacterial suspension (107 CFU/ml); controls were treated with sterile distilled water. Five weeks later plants were harvested and several growth parameters, the number and size of xylem vessels and the hydraulic conductivity of the stems were determined. Inoculated plants were shorter, with smaller leaf area and lower biomass than those of mock-inoculated plants. While the number and hydraulic radius of xylem vessels were not affected, the stem-specific (Ks) and the leaf-specific (KL) conductivity were significantly reduced by inoculation. Wilting of leaves of diseased plants was associated to the reduction of KL. Inoculated plants showed leaf spectral reflectance changes 1 week after inoculation, and 2 weeks before symptom expression, as shown by the index NDWI, an indicator of the water content of the leaves. We conclude that C. michiganensis subsp. michiganensis reduced the growth and altered plant-water relations of tomato plants by reducing the stem hydraulic conductivity. The NDWI index could be used for the early, non- destructive, diagnosis of bacterial canker.
Fil: Romero, Ana M.. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Vega, Damián. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Pizzorno, Romina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina
Fil: Cordon, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Correa, Olga S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina
description Clavibacter michiganensis subsp. michiganensis causes bacterial wilt and canker in tomato. A detailed account of pathogen effects on growth and water parameters of affected plants is lacking. Besides, the long latent period of the disease represents a limitation for its management. Our objectives were to determine the physiological and morphological changes induced by this pathogen in tomato, in relation to water stress, and to evaluate two well-known leaf spectral reflectance indices for the diagnosis of this vascular disease before symptoms expression. Experiments were conducted in a greenhouse with pot-grown plants inoculated with a bacterial suspension (107 CFU/ml); controls were treated with sterile distilled water. Five weeks later plants were harvested and several growth parameters, the number and size of xylem vessels and the hydraulic conductivity of the stems were determined. Inoculated plants were shorter, with smaller leaf area and lower biomass than those of mock-inoculated plants. While the number and hydraulic radius of xylem vessels were not affected, the stem-specific (Ks) and the leaf-specific (KL) conductivity were significantly reduced by inoculation. Wilting of leaves of diseased plants was associated to the reduction of KL. Inoculated plants showed leaf spectral reflectance changes 1 week after inoculation, and 2 weeks before symptom expression, as shown by the index NDWI, an indicator of the water content of the leaves. We conclude that C. michiganensis subsp. michiganensis reduced the growth and altered plant-water relations of tomato plants by reducing the stem hydraulic conductivity. The NDWI index could be used for the early, non- destructive, diagnosis of bacterial canker.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93205
Romero, Ana M.; Vega, Damián; Pizzorno, Romina; Cordon, Gabriela Beatriz; Correa, Olga S.; Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants; Springer; European Journal of Plant Pathology; 152; 2; 5-2018; 567-572
0929-1873
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93205
identifier_str_mv Romero, Ana M.; Vega, Damián; Pizzorno, Romina; Cordon, Gabriela Beatriz; Correa, Olga S.; Hydraulic and leaf reflectance alterations induced by Clavibacter michiganensis subsp. michiganensis on tomato plants; Springer; European Journal of Plant Pathology; 152; 2; 5-2018; 567-572
0929-1873
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s10658-018-1500-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-018-1500-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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