Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei

Autores
Gaveglio, Virginia Lucía; Giusto, Norma Maria; Pasquaré, Susana Juana
Año de publicación
2012
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Nuclear lipid metabolism gives rise to several lipid second messengers that seem to be involved in the regulation of nuclear structure and gene expression. The purpose of the present research was therefore to study the metabolic pathways involved in the metabolism of phosphatidic acid (PA) and its regulation in isolated nuclei from the central nervous system. This study was also conducted in isolated nuclei from aged animals allowing us to analyze the effects of neurodegeneration processes on PA metabolism as a result of ageing. Adult (4 mo) and aged (28 mo) rat cerebellums were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using radiolabelled substrates we demonstrated lipid phosphate phosphatases (LPPs), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), lysophosphatidate phosphohydrolase (LPAPase) and phospholipase (LPAase) as well as PA-phospholipase type A (PLA) activities. We further studied their regulation by the nuclear agonist retinoic acid (RA), which was observed to decrease DAGL and MAGL activities. Significant aged-related changes in the above-mentioned enzymatic activities as well as in its regulation by RA were observed. Taken together, our results demonstrate a RA-regulated PA metabolism in rat cerebellum nuclei which could be involved in neurodegeneration processes.
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLVIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Ciudad de Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
RETINOIC ACID
LIPID METABOLISM
NUCLEI
AGEING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247254
Acceder
id CONICETDig_c7f4c4102ae19ac022898167ba7ba58f
oai_identifier_str oai:ri.conicet.gov.ar:11336/247254
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nucleiGaveglio, Virginia LucíaGiusto, Norma MariaPasquaré, Susana JuanaRETINOIC ACIDLIPID METABOLISMNUCLEIAGEINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nuclear lipid metabolism gives rise to several lipid second messengers that seem to be involved in the regulation of nuclear structure and gene expression. The purpose of the present research was therefore to study the metabolic pathways involved in the metabolism of phosphatidic acid (PA) and its regulation in isolated nuclei from the central nervous system. This study was also conducted in isolated nuclei from aged animals allowing us to analyze the effects of neurodegeneration processes on PA metabolism as a result of ageing. Adult (4 mo) and aged (28 mo) rat cerebellums were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using radiolabelled substrates we demonstrated lipid phosphate phosphatases (LPPs), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), lysophosphatidate phosphohydrolase (LPAPase) and phospholipase (LPAase) as well as PA-phospholipase type A (PLA) activities. We further studied their regulation by the nuclear agonist retinoic acid (RA), which was observed to decrease DAGL and MAGL activities. Significant aged-related changes in the above-mentioned enzymatic activities as well as in its regulation by RA were observed. Taken together, our results demonstrate a RA-regulated PA metabolism in rat cerebellum nuclei which could be involved in neurodegeneration processes.Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLVIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularCiudad de MendozaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularInstituto de Histología y Embriología “Dr. Mario H. Burgos”2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247254Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei; XLVIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Ciudad de Mendoza; Argentina; 2012; 109-1090327-95451667-5746CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-36.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:26:07Zoai:ri.conicet.gov.ar:11336/247254instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:26:07.341CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
title Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
spellingShingle Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
Gaveglio, Virginia Lucía
RETINOIC ACID
LIPID METABOLISM
NUCLEI
AGEING
title_short Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
title_full Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
title_fullStr Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
title_full_unstemmed Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
title_sort Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei
dc.creator.none.fl_str_mv Gaveglio, Virginia Lucía
Giusto, Norma Maria
Pasquaré, Susana Juana
author Gaveglio, Virginia Lucía
author_facet Gaveglio, Virginia Lucía
Giusto, Norma Maria
Pasquaré, Susana Juana
author_role author
author2 Giusto, Norma Maria
Pasquaré, Susana Juana
author2_role author
author
dc.subject.none.fl_str_mv RETINOIC ACID
LIPID METABOLISM
NUCLEI
AGEING
topic RETINOIC ACID
LIPID METABOLISM
NUCLEI
AGEING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nuclear lipid metabolism gives rise to several lipid second messengers that seem to be involved in the regulation of nuclear structure and gene expression. The purpose of the present research was therefore to study the metabolic pathways involved in the metabolism of phosphatidic acid (PA) and its regulation in isolated nuclei from the central nervous system. This study was also conducted in isolated nuclei from aged animals allowing us to analyze the effects of neurodegeneration processes on PA metabolism as a result of ageing. Adult (4 mo) and aged (28 mo) rat cerebellums were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using radiolabelled substrates we demonstrated lipid phosphate phosphatases (LPPs), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), lysophosphatidate phosphohydrolase (LPAPase) and phospholipase (LPAase) as well as PA-phospholipase type A (PLA) activities. We further studied their regulation by the nuclear agonist retinoic acid (RA), which was observed to decrease DAGL and MAGL activities. Significant aged-related changes in the above-mentioned enzymatic activities as well as in its regulation by RA were observed. Taken together, our results demonstrate a RA-regulated PA metabolism in rat cerebellum nuclei which could be involved in neurodegeneration processes.
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLVIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Ciudad de Mendoza
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Nuclear lipid metabolism gives rise to several lipid second messengers that seem to be involved in the regulation of nuclear structure and gene expression. The purpose of the present research was therefore to study the metabolic pathways involved in the metabolism of phosphatidic acid (PA) and its regulation in isolated nuclei from the central nervous system. This study was also conducted in isolated nuclei from aged animals allowing us to analyze the effects of neurodegeneration processes on PA metabolism as a result of ageing. Adult (4 mo) and aged (28 mo) rat cerebellums were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using radiolabelled substrates we demonstrated lipid phosphate phosphatases (LPPs), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), lysophosphatidate phosphohydrolase (LPAPase) and phospholipase (LPAase) as well as PA-phospholipase type A (PLA) activities. We further studied their regulation by the nuclear agonist retinoic acid (RA), which was observed to decrease DAGL and MAGL activities. Significant aged-related changes in the above-mentioned enzymatic activities as well as in its regulation by RA were observed. Taken together, our results demonstrate a RA-regulated PA metabolism in rat cerebellum nuclei which could be involved in neurodegeneration processes.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247254
Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei; XLVIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Ciudad de Mendoza; Argentina; 2012; 109-109
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247254
identifier_str_mv Retinoic acid modifies lipid metabolism in isolated adult and aged rat cerebellum nuclei; XLVIII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Ciudad de Mendoza; Argentina; 2012; 109-109
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-36.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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